会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 3. 发明授权
    • Method of multiplex microorganism detection
    • 多重微生物检测方法
    • US08298758B2
    • 2012-10-30
    • US10584393
    • 2004-12-24
    • Naoko HorikoshiSusumu KawasakiYukio OkadaKazuko TakeshitaTakashi SameshimaShinichi KawamotoKenji Isshiki
    • Naoko HorikoshiSusumu KawasakiYukio OkadaKazuko TakeshitaTakashi SameshimaShinichi KawamotoKenji Isshiki
    • C12Q1/68
    • C12Q1/686C12Q1/689Y02A50/451
    • The present invention is to provide a multiple detection method that can detect contaminating microorganisms existing in foods, including pathogenic Escherichia coli O157, Listeria monocytogenes and Salmonella spp., with high sensitivity comparable or even superior to official methods, comprising the steps of amplifying a plural number of target genes with a single PCR reaction tube and analyzing the same. The following steps are performed consecutively: (A) a step of extracting DNA of the target microorganisms to be detected by treating with at least a lytic enzyme such as Achromopepidase and Lysozyme and/or bacteriocin having lytic activity such as Enterolysine, a surfactant and a protein denaturing agent; and (B) a step of mixing a specific primer to the target microorganisms to be detected to perform multiplex PCR. Further, it is preferable to add a step of culturing with a culture condition where 1 CFU/100 g microorganisms becomes 10.sup.3 CFU/ml or more after 18 to 48 h of culture, for example that the pH after culture becomes 5.1 or more, before the step of extracting DNA of the target microorganisms to be detected.
    • 本发明提供一种可以检测存在于食品中的污染微生物的多重检测方法,包括致病性大肠杆菌O157,单核细胞增生李斯特氏菌和沙门氏菌,具有与官方方法相当或甚至优于其他方法的高灵敏度,包括以下步骤: 使用单个PCR反应管的目标基因数目并进行分析。 连续进行以下步骤:(A)通过用至少一种裂解酶如溶酶酶和溶菌酶和/或具有溶解活性的细菌素(例如肠溶素,表面活性剂和表面活性剂)进行处理来提取要检测的目标微生物的DNA的步骤 蛋白变性剂; 和(B)将特异性引物与要检测的目标微生物混合以进行多重PCR的步骤。 此外,优选在培养18〜48小时后,添加培养条件,其中1CFU / 100g微生物变成10μFCFU / ml以上,例如培养后的pH变为5.1 或更多,在提取要检测的目标微生物的DNA的步骤之前。
    • 4. 发明授权
    • Video and audio editing system
    • 视频和音频编辑系统
    • US5911030A
    • 1999-06-08
    • US685559
    • 1996-07-24
    • Tsuneyuki KikuchiTakashi Sameshima
    • Tsuneyuki KikuchiTakashi Sameshima
    • H04N5/91G06F17/30G11B27/02G11B27/036G11B27/10H04N9/79
    • G06F17/30017
    • A video and audio editing system which eliminates failures in the fetching of video or audio data. The video and audio editing system also accounts for the silence which occurs at the top of audio data and the problems which arise when compressed video and audio data are reproduced simultaneously but not synchronously. The system includes a start time table and an end time table for storing a processing start time and a processing end time, respectively, for each scene to be fetched, a time lag inputting apparatus for inputting time lags in fetching of an external apparatus and an audio board, a table updating apparatus for updating contents of the start and end time tables based on the inputted time lags, and a controller for referring to the contents of the start and end time tables to control fetching start and end times of video data and audio data or control reading start and end times from the storage medium and fetching or reading out the audio data skipping silence data corresponding to the time lags.
    • 视频和音频编辑系统消除了获取视频或音频数据的失败。 视频和音频编辑系统还解决了在音频数据的顶部发生的静音以及当压缩的视频和音频数据被同时再现而不是同步地再现时出现的问题。 该系统包括分别用于存储要获取的每个场景的处理开始时间和处理结束时间的开始时间表和结束时间表,用于在取出外部设备时输入时间滞后的时间延迟输入装置和 音频板,用于基于输入的时间滞后来更新开始和结束时间表的内容的表格更新装置,以及用于参考开始和结束时间表的内容以控制获取视频数据的开始和结束时间的控制器, 音频数据或控制从存储介质读取开始和结束时间,并且获取或读出跳过与时间滞后相对应的静音数据的音频数据。
    • 5. 发明申请
    • Method of Multiplex Microorganism Detection
    • 多重微生物检测方法
    • US20080014578A1
    • 2008-01-17
    • US10584393
    • 2004-12-24
    • Naoko HorikoshiSusumu KawasakiYukio OkadaKazuko TakeshitaTakashi SameshimaShinichi KawamotoKenji Isshiki
    • Naoko HorikoshiSusumu KawasakiYukio OkadaKazuko TakeshitaTakashi SameshimaShinichi KawamotoKenji Isshiki
    • C12Q1/68
    • C12Q1/686C12Q1/689Y02A50/451
    • The present invention is to provide a multiple detection method that can detect contaminating microorganisms existing in foods, including pathogenic Escherichia coli O157, Listeria monocytogenes and Salmonella spp., with high sensitivity comparable or even superior to official methods, comprising the steps of amplifying a plural number of target genes with a single PCR reaction tube and analyzing the same. The following steps are performed consecutively: (A) a step of extracting DNA of the target microorganisms to be detected by treating with at least a lytic enzyme such as Achromopepidase and Lyzocyme and/or bacteriocin having lytic activity such as Enterolysine, a surfactant and a protein denaturing agent; and (B) a step of mixing a specific primer to the target microorganisms to be detected to perform multiplex PCR. Further, it is preferable to add a step of culturing with a culture condition where 1 CFU/100 g microorganisms becomes 103 CFU/ml or more after 18 to 48 h of culture, for example that the pH after culture becomes 5.1 or more, before the step of extracting DNA of the target microorganisms to be detected.
    • 本发明提供一种可以检测存在于食品中的污染微生物的多重检测方法,包括致病性大肠杆菌O157,单核细胞增生李斯特氏菌和沙门氏菌,具有与官方方法相当或甚至优于其他方法的高灵敏度,包括以下步骤: 使用单个PCR反应管的目标基因数目并进行分析。 连续进行以下步骤:(A)通过用至少溶解酶如具有溶解活性的溶色酶和溶菌酶等溶菌酶和表面活性剂和表面活性剂的溶解活性的溶菌酶和/或细菌素进行处理来提取要检测的目标微生物的DNA的步骤 蛋白变性剂; 和(B)将特异性引物与要检测的目标微生物混合以进行多重PCR的步骤。 此外,优选在培养18〜48小时后,加入培养条件,其中1CFU / 100g微生物变为10 3 CFU / ml以上,例如pH 在提取要检测的目标微生物的DNA的步骤之前,培养变为5.1以上。