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1. 发明授权

US08298758B2 Method of multiplex microorganism detection 有权
标题翻译：多重微生物检测方法
公开(公告)号：US08298758B2
公开(公告)日：2012-10-30
申请号：US10584393
申请日：2004-12-24
申请人： Naoko Horikoshi , Susumu Kawasaki , Yukio Okada , Kazuko Takeshita , Takashi Sameshima , Shinichi Kawamoto , Kenji Isshiki
发明人： Naoko Horikoshi , Susumu Kawasaki , Yukio Okada , Kazuko Takeshita , Takashi Sameshima , Shinichi Kawamoto , Kenji Isshiki
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/689 , Y02A50/451
摘要： The present invention is to provide a multiple detection method that can detect contaminating microorganisms existing in foods, including pathogenic Escherichia coli O157, Listeria monocytogenes and Salmonella spp., with high sensitivity comparable or even superior to official methods, comprising the steps of amplifying a plural number of target genes with a single PCR reaction tube and analyzing the same. The following steps are performed consecutively: (A) a step of extracting DNA of the target microorganisms to be detected by treating with at least a lytic enzyme such as Achromopepidase and Lysozyme and/or bacteriocin having lytic activity such as Enterolysine, a surfactant and a protein denaturing agent; and (B) a step of mixing a specific primer to the target microorganisms to be detected to perform multiplex PCR. Further, it is preferable to add a step of culturing with a culture condition where 1 CFU/100 g microorganisms becomes 10.sup.3 CFU/ml or more after 18 to 48 h of culture, for example that the pH after culture becomes 5.1 or more, before the step of extracting DNA of the target microorganisms to be detected.
摘要翻译：本发明提供一种可以检测存在于食品中的污染微生物的多重检测方法，包括致病性大肠杆菌O157，单核细胞增生李斯特氏菌和沙门氏菌，具有与官方方法相当或甚至优于其他方法的高灵敏度，包括以下步骤：使用单个PCR反应管的目标基因数目并进行分析。连续进行以下步骤：（A）通过用至少一种裂解酶如溶酶酶和溶菌酶和/或具有溶解活性的细菌素（例如肠溶素，表面活性剂和表面活性剂）进行处理来提取要检测的目标微生物的DNA的步骤蛋白变性剂; 和（B）将特异性引物与要检测的目标微生物混合以进行多重PCR的步骤。此外，优选在培养18〜48小时后，添加培养条件，其中1CFU / 100g微生物变成10μFCFU / ml以上，例如培养后的pH变为5.1 或更多，在提取要检测的目标微生物的DNA的步骤之前。

2. 发明授权

US6163646A Apparatus for a synchronized playback of audio-video signals 失效
标题翻译：用于音频 - 视频信号的同步回放的装置
公开(公告)号：US6163646A
公开(公告)日：2000-12-19
申请号：US959196
申请日：1997-10-28
申请人： Mitsumasa Tanaka , Takashi Sameshima , Masaru Terashima , Hideki Sawada
发明人： Mitsumasa Tanaka , Takashi Sameshima , Masaru Terashima , Hideki Sawada
IPC分类号： H04N5/92 , H04N5/781 , H04N5/85 , H04N5/93 , H04N9/804 , H04N9/806 , H04N9/877 , H04N5/76
CPC分类号： H04N21/4307 , H04N5/781 , H04N5/85 , H04N9/8047 , H04N9/8063 , H04N9/877
摘要： To provide an apparatus for a synchronized playback of compressed digital data of audio-video signals with a simple configuration to be applied to a system having comparatively low data processing ability, independent of resolution of a software timer of the system, the apparatus of the invention obtains a reference time indicating reproduction time passage of the audio signal component from reproduced data amount of the audio signal component, and detects progress of the video signal component in reference to the reference time.
摘要翻译：为了提供用于以简单的配置来同步回放音频 - 视频信号的压缩数字数据的装置，以适用于具有相对低的数据处理能力的系统，与系统的软件定时器的分辨率无关，本发明的装置从音频信号分量的再现数据量获得指示音频信号分量的再现时间通过的参考时间，并且参考参考时间检测视频信号分量的进度。

3. 发明申请

US20080014578A1 Method of Multiplex Microorganism Detection 有权
标题翻译：多重微生物检测方法
公开(公告)号：US20080014578A1
公开(公告)日：2008-01-17
申请号：US10584393
申请日：2004-12-24
申请人： Naoko Horikoshi , Susumu Kawasaki , Yukio Okada , Kazuko Takeshita , Takashi Sameshima , Shinichi Kawamoto , Kenji Isshiki
发明人： Naoko Horikoshi , Susumu Kawasaki , Yukio Okada , Kazuko Takeshita , Takashi Sameshima , Shinichi Kawamoto , Kenji Isshiki
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/689 , Y02A50/451
摘要： The present invention is to provide a multiple detection method that can detect contaminating microorganisms existing in foods, including pathogenic Escherichia coli O157, Listeria monocytogenes and Salmonella spp., with high sensitivity comparable or even superior to official methods, comprising the steps of amplifying a plural number of target genes with a single PCR reaction tube and analyzing the same. The following steps are performed consecutively: (A) a step of extracting DNA of the target microorganisms to be detected by treating with at least a lytic enzyme such as Achromopepidase and Lyzocyme and/or bacteriocin having lytic activity such as Enterolysine, a surfactant and a protein denaturing agent; and (B) a step of mixing a specific primer to the target microorganisms to be detected to perform multiplex PCR. Further, it is preferable to add a step of culturing with a culture condition where 1 CFU/100 g microorganisms becomes 103 CFU/ml or more after 18 to 48 h of culture, for example that the pH after culture becomes 5.1 or more, before the step of extracting DNA of the target microorganisms to be detected.
摘要翻译：本发明提供一种可以检测存在于食品中的污染微生物的多重检测方法，包括致病性大肠杆菌O157，单核细胞增生李斯特氏菌和沙门氏菌，具有与官方方法相当或甚至优于其他方法的高灵敏度，包括以下步骤：使用单个PCR反应管的目标基因数目并进行分析。连续进行以下步骤：（A）通过用至少溶解酶如具有溶解活性的溶色酶和溶菌酶等溶菌酶和表面活性剂和表面活性剂的溶解活性的溶菌酶和/或细菌素进行处理来提取要检测的目标微生物的DNA的步骤蛋白变性剂; 和（B）将特异性引物与要检测的目标微生物混合以进行多重PCR的步骤。此外，优选在培养18〜48小时后，加入培养条件，其中1CFU / 100g微生物变为10 3 CFU / ml以上，例如pH 在提取要检测的目标微生物的DNA的步骤之前，培养变为5.1以上。

4. 发明授权

US6043851A Image and sound synchronizing reproduction apparatus and method of the same 失效
标题翻译：图像和声音同步再现装置及其方法
公开(公告)号：US6043851A
公开(公告)日：2000-03-28
申请号：US6658
申请日：1998-01-13
申请人： Hideki Sawada , Takashi Sameshima , Masaru Terashima , Mitsumasa Tanaka
发明人： Hideki Sawada , Takashi Sameshima , Masaru Terashima , Mitsumasa Tanaka
IPC分类号： H04N5/92 , G11B27/10 , H04N5/04 , H04N5/60 , H04N5/93 , H04N7/52 , H04N19/00 , H04N19/102 , H04N19/132 , H04N19/196 , H04N19/423 , H04N19/44 , H04N19/503 , H04N19/577 , H04N19/587 , H04N19/70 , H04N19/85 , H04N9/475
CPC分类号： H04N21/4307 , H04N5/04 , H04N5/602 , H04N5/4401
摘要： An image and sound synchronizing reproduction apparatus includes a reference time calculation circuit for calculating a reference time in response to a data amount of decoded sound data, a delay detecting circuit calculating number of image frames to be processed practically in the decoding process in response to the reference time and comparing the number of image frames with number of frames practically processed in the decoding process, for detecting a delay of image decoding process, a frame-removing control circuit for performing a discriminating process of frames to be omitted the decoding process in response to the number of delay frames detected by the delay detecting circuit, and an image data input control circuit for performing an omission to read compressed image data corresponding to the frames discriminated by the frame-removing control circuit, controlling finely the image and sound synchronizing reproduction regardless of the structure of compressing coded data.
摘要翻译：图像和声音同步再现装置包括：基准时间计算电路，用于响应于解码声音数据的数据量来计算基准时间;延迟检测电路，响应于所述解码处理实际上计算待处理的图像帧数; 参考时间，并且将图像帧的数量与在解码处理中实际处理的帧的数目进行比较，以检测图像解码处理的延迟;帧去除控制电路，用于响应于解码处理执行帧的识别处理延迟检测电路检测的延迟帧的数量，以及图像数据输入控制电路，用于执行省略以读取与由帧除去控制电路鉴别的帧相对应的压缩图像数据，精细地控制图像和声音同步再现而不管压缩编码数据的结构如何。

5. 发明授权

US5911030A Video and audio editing system 失效
标题翻译：视频和音频编辑系统
公开(公告)号：US5911030A
公开(公告)日：1999-06-08
申请号：US685559
申请日：1996-07-24
申请人： Tsuneyuki Kikuchi , Takashi Sameshima
发明人： Tsuneyuki Kikuchi , Takashi Sameshima
IPC分类号： H04N5/91 , G06F17/30 , G11B27/02 , G11B27/036 , G11B27/10 , H04N9/79
CPC分类号： G06F17/30017
摘要： A video and audio editing system which eliminates failures in the fetching of video or audio data. The video and audio editing system also accounts for the silence which occurs at the top of audio data and the problems which arise when compressed video and audio data are reproduced simultaneously but not synchronously. The system includes a start time table and an end time table for storing a processing start time and a processing end time, respectively, for each scene to be fetched, a time lag inputting apparatus for inputting time lags in fetching of an external apparatus and an audio board, a table updating apparatus for updating contents of the start and end time tables based on the inputted time lags, and a controller for referring to the contents of the start and end time tables to control fetching start and end times of video data and audio data or control reading start and end times from the storage medium and fetching or reading out the audio data skipping silence data corresponding to the time lags.
摘要翻译：视频和音频编辑系统消除了获取视频或音频数据的失败。视频和音频编辑系统还解决了在音频数据的顶部发生的静音以及当压缩的视频和音频数据被同时再现而不是同步地再现时出现的问题。该系统包括分别用于存储要获取的每个场景的处理开始时间和处理结束时间的开始时间表和结束时间表，用于在取出外部设备时输入时间滞后的时间延迟输入装置和音频板，用于基于输入的时间滞后来更新开始和结束时间表的内容的表格更新装置，以及用于参考开始和结束时间表的内容以控制获取视频数据的开始和结束时间的控制器，音频数据或控制从存储介质读取开始和结束时间，并且获取或读出跳过与时间滞后相对应的静音数据的音频数据。

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