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1. 发明申请

US20060240445A1 Multiplex SNP typing by bioluminometric assay coupled with terminator incorporation 审中-公开
标题翻译：通过生物光谱测定与终止子掺入进行复制SNP分型
公开(公告)号：US20060240445A1
公开(公告)日：2006-10-26
申请号：US11209652
申请日：2005-08-24
申请人： Hideki Kambara , Guohua Zhou , Tomoharu Kajiyama
发明人： Hideki Kambara , Guohua Zhou , Tomoharu Kajiyama
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6823 , C12Q1/6869 , C12Q2565/301 , C12Q2563/103 , C12Q2525/186
摘要： The present invention provides a method for analyzing a nucleotide sequence comprising the steps of: carrying out complementary strand synthesis by adding at least one of four kinds of ddNTP corresponding to nucleotides A, G, T, and C, or derivatives thereof to a reaction vessel containing a nucleic acid sample to extend one nucleotide at a target site; performing a bioluminescent reaction with the use of ATP formed from released pyrophosphate as a reaction substrate; and typing the target site by determining the presence or absence of the complementary strand synthesis based on a result of the bioluminescent reaction. The method of the present invention allows multiplex SNPs to be typed in one reaction vessel
摘要翻译：本发明提供了分析核苷酸序列的方法，包括以下步骤：通过将对应于核苷酸A，G，T和C的四种ddNTP或其衍生物中的至少一种或其衍生物加入到反应容器中来进行互补链合成含有核酸样品以在靶位点延伸一个核苷酸; 使用由释放的焦磷酸盐形成的ATP作为反应底物进行生物发光反应; 并通过基于生物发光反应的结果确定互补链合成的存在或不存在来分类靶位点。本发明的方法允许在一个反应容器中输入多重SNP

2. 发明申请

US20070166729A1 Method and reagent for sequencing 审中-公开
标题翻译：测序方法和试剂
公开(公告)号：US20070166729A1
公开(公告)日：2007-07-19
申请号：US11542246
申请日：2006-10-04
申请人： Hideki Kambara , Guohua Zhou , Tomoharu Kajiyama , Shigeya Suzuki
发明人： Hideki Kambara , Guohua Zhou , Tomoharu Kajiyama , Shigeya Suzuki
IPC分类号： C12Q1/68 , C12Q1/66
CPC分类号： C12Q1/66 , C12Q1/6869 , C12Q2565/301
摘要： The present invention provides: a method for nucleic acid analysis including the steps of subjecting a reaction solution containing a sample nucleic acid to complementary strand synthesis with the sample nucleic acid as a template, reacting pyrophosphate produced in the complementary strand synthesis with 30 to 800 μM AMP in the coexistence of pyruvate phosphate dikinase to produce ATP, performing luciferase reaction with the ATP as a reaction substrate, and detecting chemiluminescence generated in the luciferase reaction to determine the presence or absence of the complementary strand synthesis; and a kit therefor.
摘要翻译：本发明提供了一种核酸分析方法，包括以下步骤：将含有样品核酸的反应溶液与样品核酸作为模板进行互补链合成，使互补链合成中产生的焦磷酸与30〜800μm AMP在丙二酸磷酸二激酶共存产生ATP，与ATP作为反应底物进行荧光素酶反应，并检测在荧光素酶反应中产生的化学发光，以确定互补链合成的存在或不存在; 和一个工具包。

3. 发明申请

US20070054283A1 DNA sequencing method using step by step reaction 审中-公开
标题翻译： DNA测序方法采用逐步反应
公开(公告)号：US20070054283A1
公开(公告)日：2007-03-08
申请号：US11356187
申请日：2006-02-17
申请人： Akihiko Kishimoto , Hideki Kambara , Tomoharu Kajiyama , Guohua Zhou
发明人： Akihiko Kishimoto , Hideki Kambara , Tomoharu Kajiyama , Guohua Zhou
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q2537/149
摘要： The present invention provides an inexpensive DNA sequencing method with high-sensitivity. The method of the present invention comprising the steps of, adding an given amount of dATP for step by step complementary strand synthesis and subtracting the background luminescence intensity caused by dATP from the measured luminescence intensity to obtain the luminescence intensity involved in complementary strand synthesis.
摘要翻译：本发明提供了具有高灵敏度的便宜的DNA测序方法。本发明的方法包括以下步骤：加入给定量的dATP进行逐步互补链合成，并从测量的发光强度减去由dATP引起的背景发光强度，以获得互补链合成中所涉及的发光强度。

4. 发明授权

US08802367B2 Methods for quantitative cDNA analysis in single-cell 有权
标题翻译：单细胞定量cDNA分析方法
公开(公告)号：US08802367B2
公开(公告)日：2014-08-12
申请号：US11783575
申请日：2007-04-10
申请人： Kiyomi Taniguchi , Hideki Kambara , Tomoharu Kajiyama
发明人： Kiyomi Taniguchi , Hideki Kambara , Tomoharu Kajiyama
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6811 , C12N15/1096 , C12Q1/6809 , C12Q1/6834 , C12Q2565/537 , C12Q2525/173 , C12Q2521/301 , C12Q2561/113
摘要： It is an object to provide a method of suitably analyzing the amount of gene expression of a single-cell.A method of detecting a nucleic acid comprising a step of sampling a single-cell from a sample containing at least a single-cell, a cell lysis step of lysing cell membrane of the sampled single-cell and extracting nucleic acids from the cell, a DNase treatment step of degrading DNA of the extracted nucleic acids with DNase, a step of hybridizing mRNA of the total RNA contained in the single-cell with oligo (dT) fixed onto a carrier, a step of performing reverse transcription of the mRNA hybridized with the oligo (dT) to fix cDNA derived from the single-cell onto the carrier, thereby preparing a single-cell derived cDNA library fixed onto a carrier, and a step of amplifying cDNA fixed onto the carrier and simultaneously detecting an amplification amount of the cDNA.
摘要翻译：本发明的目的是提供适当分析单细胞的基因表达量的方法。一种检测核酸的方法，包括从含有至少单细胞的样品中取样单细胞的步骤，裂解采样的单细胞的细胞膜并从细胞中提取核酸的细胞裂解步骤用DNase降解提取的核酸的DNA的DNA酶处理步骤，将单细胞中含有的总RNA的mRNA与固定在载体上的寡聚（dT）杂交的步骤，将与mRNA杂交的mRNA进行逆转录的步骤将来自单细胞的cDNA固定在载体上的寡核苷酸（dT），从而制备固定在载体上的单细胞衍生的cDNA文库，以及扩增固定在载体上的cDNA并同时检测扩增量的步骤 cDNA。

5. 发明申请

US20060141494A1 Luminescence detection apparatus 审中-公开
标题翻译：发光检测装置
公开(公告)号：US20060141494A1
公开(公告)日：2006-06-29
申请号：US11209702
申请日：2005-08-24
申请人： Hideki Kambara , Tomoharu Kajiyama , Kunio Harada , Masao Kamahori
发明人： Hideki Kambara , Tomoharu Kajiyama , Kunio Harada , Masao Kamahori
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： G01N21/03 , G01N21/6428 , G01N21/6452 , G01N21/76
摘要： An object of the present invention is to provide a luminescence detection apparatus compact in size which is capable of conveniently determining DNA base sequences at a low cost. According to the present invention, a luminescence detection apparatus 1 is provided comprising: a plurality of reaction cells 6 each having a transparent bottom portion; a solution-dispensing portion 19 equipped with capillaries 18 positioned above the reaction cells 6 and put into a one-to-one correspondence with the reaction cells 6; and a light-detecting portion 29 having a plurality of light-sensing elements 24 put into a one-to-one correspondence with the reaction cells 6 and arranged in proximity to the bottom surfaces of the reaction cells 6, wherein the a plurality of light-sensing elements 24 of the light-detecting portion 29 detect respective luminescences in the reaction cells 6 generated by injecting reagent solutions from the solution-dispensing portion 19 to the reaction cells 6.
摘要翻译：本发明的目的是提供一种尺寸紧凑的发光检测装置，其能够以低成本方便地确定DNA碱基序列。根据本发明，提供一种发光检测装置1，其包括：多个反应池6，每个反应池6具有透明底部; 溶液分配部分19，其配备有位于反应单元6上方并与反应单元6一一对应的毛细管18; 以及具有多个光检测元件24的光检测部分29，其与反应单元6一一对应地布置在反应单元6的底表面附近，其中多个光光检测部分29的光敏元件24检测通过从溶液分配部分19将反应池6注入试剂溶液而产生的反应池6中的相应发光。

6. 发明申请

US20090215162A1 Nucleic acid amplification device 有权
标题翻译：核酸扩增装置
公开(公告)号：US20090215162A1
公开(公告)日：2009-08-27
申请号：US12292167
申请日：2008-11-13
申请人： Tomoharu Kajiyama , Masataka Shirai , Hideki Kambara
发明人： Tomoharu Kajiyama , Masataka Shirai , Hideki Kambara
IPC分类号： C12M1/40
CPC分类号： B01L3/50851 , B01L3/5025 , B01L3/50857 , B01L2200/0668 , B01L2300/0819
摘要： This invention provides a nucleic acid amplification device whereby the abundance of a target molecule can be maintained before and after a step of separately amplifying a sample such that highly accurate analysis results that can be applied to gene expression analysis can be obtained. Also, a nucleic acid amplification device having a structure in which a plurality of minute reaction cells each comprising a set of a bead-retaining space capable of retaining a single analysis bead and a reagent reaction space retaining no bead but having a volume that is large enough to cause a reagent reaction therein are positioned so as to form a planar face is provided.
摘要翻译：本发明提供了一种核酸扩增装置，其可以在分别扩增样品的步骤之前和之后维持目标分子的丰度，使得可以获得可应用于基因表达分析的高精度分析结果。另外，具有这样的结构的核酸扩增装置，其中多个微小反应单元各自包含一组能够保留单个分析珠的珠保留空间和不保留珠而具有大体积的试剂反应空间足以使其中的试剂反应定位成形成平面。

7. 发明申请

US20080318244A1 Large-scale parallel nucleic acid analysis method 审中-公开
标题翻译：大规模并行核酸分析方法
公开(公告)号：US20080318244A1
公开(公告)日：2008-12-25
申请号：US12213448
申请日：2008-06-19
申请人： Hiroko Matsunaga , Hideki Kambara , Tomoharu Kajiyama
发明人： Hiroko Matsunaga , Hideki Kambara , Tomoharu Kajiyama
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6834 , C12Q1/6844 , C12Q2537/143 , C12Q2525/191 , C12Q2533/101 , C12Q2565/537
摘要： It is intended to provide a technique for amplifying, individually and in parallel, nucleic acids contained in a mixture of plural kinds of nucleic acid samples. The present invention provides a nucleic acid analysis method comprising amplification means, whereby amplification reaction is performed in a reaction solution comprising a homogeneous solvent and comprising at least plural template nucleic acids and solid phase carriers comprising one or more kinds of amplification probes immobilized on the surface, to prevent amplified products attributed to two or more template nucleic acids from being replicated in one solid phase carrier. According to the present invention, plural kinds of analyte nucleic acid samples in a mixed state can be amplified individually and in parallel. This method achieves one solid phase carrier-one nucleic acid. Therefore, a higher density of solid phase carriers with obtained amplified products is easily achieved, leading to improved throughput of amplified product analysis. Reactions in all the amplification reaction steps are performed under homogeneous solvent conditions. Therefore, the method of the present invention is performed by convenient procedures and as such, is suitable to automation.
摘要翻译：旨在提供用于扩增多种核酸样品的混合物中包含的核酸的单独和并行扩增的技术。本发明提供了包含扩增方法的核酸分析方法，其中扩增反应在包含均相溶剂并包含至少多个模板核酸的反应溶液中进行，并且包含固定在表面上的一种或多种扩增探针的固相载体，以防止归因于两种或更多种模板核酸的扩增产物被复制在一种固相载体中。根据本发明，可以单独并行地扩增处于混合状态的多种分析物核酸样品。该方法实现了一种固相载体一核酸。因此，容易获得具有获得的扩增产物的较高密度的固相载体，从而提高扩增产物分析的产量。所有扩增反应步骤中的反应在均相溶剂条件下进行。因此，本发明的方法通过方便的程序进行，因此适用于自动化。

8. 外观设计

USD546966S1 Reagent container 有权
标题翻译：试剂容器
公开(公告)号：USD546966S1
公开(公告)日：2007-07-17
申请号：US29255840
申请日：2006-03-14
申请人： Tomoharu Kajiyama , Hideki Kambara , Kunio Harada
设计人： Tomoharu Kajiyama , Hideki Kambara , Kunio Harada

9. 发明申请

US20070122310A1 Small size gene analysis apparatus 有权
标题翻译：小尺寸基因分析仪
公开(公告)号：US20070122310A1
公开(公告)日：2007-05-31
申请号：US11601725
申请日：2006-11-20
申请人： Tomoharu Kajiyama , Hideki Kambara , Kunio Harada
发明人： Tomoharu Kajiyama , Hideki Kambara , Kunio Harada
IPC分类号： G01N21/00
CPC分类号： B01L3/0241 , B01J2219/00376 , B01J2219/00378 , B01J2219/00416 , B01J2219/00418 , B01J2219/00484 , B01L2200/0621 , B01L2200/16 , B01L2300/0838 , B01L2400/0487 , B01L2400/049 , G01N35/1072 , Y10T436/2575
摘要： By the conventional technique for dispensing more than one reagents accurately, the system is complicated and thus a compact and inexpensive system is difficult to realize. In the present invention, the pressurized dispensing system utilizing a capillary is realized, and in addition, in order to reduce the leakage of reagents different from the reagent dispensed, by forming air layers at the tips of the capillaries after dispensing, a compact, simple, inexpensive analysis apparatus is realized.
摘要翻译：通过用于准确地分配多种试剂的常规技术，系统复杂，因此难以实现紧凑且廉价的系统。在本发明中，实现了利用毛细管的加压分配系统，此外，为了减少与分配的试剂不同的试剂的泄漏，通过在分配后在毛细管的末端形成空气层，结构紧凑，简单，实现了廉价的分析装置。

10. 发明申请

US20110244448A1 DNA DETECTING APPARATUS, DNA DETECTING DEVICE AND DNA DETECTING METHOD 审中-公开
标题翻译： DNA检测装置，DNA检测装置和DNA检测方法
公开(公告)号：US20110244448A1
公开(公告)日：2011-10-06
申请号：US13058934
申请日：2009-08-31
申请人： Masataka Shirai , Tomoharu Kajiyama , Hideki Kambara
发明人： Masataka Shirai , Tomoharu Kajiyama , Hideki Kambara
IPC分类号： C12Q1/68 , C12M1/40
CPC分类号： G01N21/76 , B01L3/502707 , B01L2200/0668 , B01L2300/0636 , B01L2300/0816 , B01L2300/0877 , C09B23/0025 , C09B23/0066 , C12Q1/66 , C12Q1/6825 , G01N33/5432 , G01N33/582
摘要： A chemiluminescence detecting apparatus includes a thing called a plate where many reaction chambers are one-dimensionally or two-dimensionally arranged, and enzymes packed in a gel and DNA-immobilized beads are simultaneously charged into the reaction chambers, thereby trapping a large amount of enzymes in the reaction chambers, improving enzyme activity and achieving high sensitive pyrosequencing.
摘要翻译：化学发光检测装置包括称为板的物体，其中许多反应室是一维或二维排列的，并且装入凝胶中的酶和固定有DNA的珠粒同时装入反应室中，从而捕获大量的酶在反应室中，提高酶活性并实现高灵敏度的焦磷酸测序。

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