专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US08759506B2 Expression enhancing intron sequences 失效
标题翻译：表达增强内含子序列
公开(公告)号：US08759506B2
公开(公告)日：2014-06-24
申请号：US13241493
申请日：2011-09-23
申请人： Hee-Sook Song , Christian Dammann , Marc Morra , Jeffrey A. Brown , Liqun Xing , Hongmei Jia
发明人： Hee-Sook Song , Christian Dammann , Marc Morra , Jeffrey A. Brown , Liqun Xing , Hongmei Jia
IPC分类号： A01H5/00 , C07H21/04 , C12N15/113 , C12N15/63 , C12N15/82 , C12N1/21
CPC分类号： C12N15/8241 , C12N15/8216 , C12N15/8225 , C12N15/8227 , C12N15/8234
摘要： The invention relates to methods for the identification and use of introns with gene expression enhancing properties. The teaching of this invention enables the identification of introns causing intron-mediated enhancement (IME) of gene expression. The invention furthermore relates to recombinant expression construct and vectors comprising said IME-introns operably linked with a promoter sequence and a nucleic acid sequence. The present invention also relates to transgenic plants and plant cells transformed with these recombinant expression constructs or vectors, to cultures, parts or propagation material derived there from, and to the use of same for the preparation of foodstuffs, animal feeds, seed, pharmaceuticals or fine chemicals, to improve plant biomass, yield, or provide desirable phenotypes.
摘要翻译：本发明涉及鉴定和使用具有基因表达增强特性的内含子的方法。本发明的教导能够鉴定引起基因表达的内含子介导的增强（IME）的内含子。本发明还涉及重组表达构建体和包含与启动子序列和核酸序列可操作地连接的所述IME-内含子的载体。本发明还涉及用这些重组表达构建体或载体转化的转基因植物和植物细胞，从其衍生的培养物，部分或繁殖材料，以及其用于制备食品，动物饲料，种子，药物或精细化学品，以改善植物生物量，产量或提供期望的表型。

2. 发明申请

US20090144863A1 Expression Enhancing Intron Sequences 有权
标题翻译：表达增强内含子序列
公开(公告)号：US20090144863A1
公开(公告)日：2009-06-04
申请号：US11885988
申请日：2006-03-07
申请人： Hee-Sook Song , Christian Dammann , Marc Morra , Jeffrey A. Brown , Liqun Xing , Hongmei Jia
发明人： Hee-Sook Song , Christian Dammann , Marc Morra , Jeffrey A. Brown , Liqun Xing , Hongmei Jia
IPC分类号： A01H5/00 , C12Q1/68 , C40B30/00 , C12N15/00 , C12N15/11 , C12N5/04
CPC分类号： C12N15/8241 , C12N15/8216 , C12N15/8225 , C12N15/8227 , C12N15/8234
摘要： The invention relates to methods for the identification and use of introns with gene expression enhancing properties. The teaching of this invention enables the identification of introns causing intron-mediated enhancement (IME) of gene expression. The invention furthermore relates to recombinant expression construct and vectors comprising said IME-introns operably linked with a promoter sequence and a nucleic acid sequence. The present invention also relates to transgenic plants and plant cells transformed with these recombinant expression constructs or vectors, to cultures, parts or propagation material derived there from, and to the use of same for the preparation of foodstuffs, animal feeds, seed, pharmaceuticals or fine chemicals, to improve plant biomass, yield, or provide desirable phenotypes.
摘要翻译：本发明涉及鉴定和使用具有基因表达增强特性的内含子的方法。本发明的教导能够鉴定引起基因表达的内含子介导的增强（IME）的内含子。本发明还涉及重组表达构建体和包含与启动子序列和核酸序列可操作地连接的所述IME-内含子的载体。本发明还涉及用这些重组表达构建体或载体转化的转基因植物和植物细胞，从其衍生的培养物，部分或繁殖材料，以及其用于制备食品，动物饲料，种子，药物或精细化学品，以改善植物生物量，产量或提供期望的表型。

3. 发明申请

US20120054922A1 EXPRESSION ENHANCING INTRON SEQUENCES 失效
标题翻译：表达增强内容序列
公开(公告)号：US20120054922A1
公开(公告)日：2012-03-01
申请号：US13241493
申请日：2011-09-23
申请人： Hee-Sook Song , Christian Dammann , Marc Morra , Jeffrey A. Brown , Liqun Xing , Hongmei Jia
发明人： Hee-Sook Song , Christian Dammann , Marc Morra , Jeffrey A. Brown , Liqun Xing , Hongmei Jia
IPC分类号： C40B30/04 , C12N1/21 , C40B30/02 , C12N1/19 , C12N5/10 , A01H5/00 , C12N15/63 , C12N1/15
CPC分类号： C12N15/8241 , C12N15/8216 , C12N15/8225 , C12N15/8227 , C12N15/8234
摘要： The invention relates to methods for the identification and use of introns with gene expression enhancing properties. The teaching of this invention enables the identification of introns causing intron-mediated enhancement (IME) of gene expression. The invention furthermore relates to recombinant expression construct and vectors comprising said IME-introns operably linked with a promoter sequence and a nucleic acid sequence. The present invention also relates to transgenic plants and plant cells transformed with these recombinant expression constructs or vectors, to cultures, parts or propagation material derived there from, and to the use of same for the preparation of foodstuffs, animal feeds, seed, pharmaceuticals or fine chemicals, to improve plant biomass, yield, or provide desirable phenotypes.
摘要翻译：本发明涉及鉴定和使用具有基因表达增强特性的内含子的方法。本发明的教导能够鉴定引起基因表达的内含子介导的增强（IME）的内含子。本发明还涉及重组表达构建体和包含与启动子序列和核酸序列可操作地连接的所述IME-内含子的载体。本发明还涉及用这些重组表达构建体或载体转化的转基因植物和植物细胞，从其衍生的培养物，部分或繁殖材料，以及其用于制备食品，动物饲料，种子，药物或精细化学品，以改善植物生物量，产量或提供期望的表型。

4. 发明授权

US07989678B2 Disarmed agrobacterium strains, Ri-plasmids, and methods of transformation based thereon 有权
标题翻译：解除农杆菌菌株，Ri质粒和其上的转化方法
公开(公告)号：US07989678B2
公开(公告)日：2011-08-02
申请号：US11661703
申请日：2005-08-31
申请人： Luke Mankin , Dwight-Steven Hill , Paula Olhoft , Effie Toren , Jeffrey A. Brown , Liqun Xing , Huihua Fu , Lesley Ireland , Hongmei Jia , Hee-Sook Song , Allan Richard Wenck , Larry Nea
发明人： Luke Mankin , Dwight-Steven Hill , Paula Olhoft , Effie Toren , Jeffrey A. Brown , Liqun Xing , Huihua Fu , Lesley Ireland , Hongmei Jia , Hee-Sook Song , Allan Richard Wenck , Larry Nea
IPC分类号： C12N15/84 , C12N1/20 , C12N1/21 , C12N15/31
CPC分类号： C07K14/195 , C12N15/8205
摘要： The present invention relates “disarmed” strain variants of Agrobacterium strain K599 (NCPPB 2659), “disarmed” plasmid variants of the Ri-plasmid pRi2659, and derivatives thereof, and methods employing these strains and plasmids in plant transformation.
摘要翻译：本发明涉及农杆菌菌株K599（NCPPB2659）的“撤防”菌株变体，Ri质粒pRi2659的“解除武装”质粒变体及其衍生物，以及在植物转化中使用这些菌株和质粒的方法。

5. 发明授权

US08465915B2 Method for isolation of transcription termination sequences 失效
标题翻译：分离转录终止序列的方法
公开(公告)号：US08465915B2
公开(公告)日：2013-06-18
申请号：US11659126
申请日：2005-07-30
申请人： Hee-Sook Song , Michael Kock , Jeffrey A. Brown , Linda Patricia Loyall , Liqun Xing , Hongmei Jia , John McMillan , Lesley Ireland
发明人： Hee-Sook Song , Michael Kock , Jeffrey A. Brown , Linda Patricia Loyall , Liqun Xing , Hongmei Jia , John McMillan , Lesley Ireland
IPC分类号： C12Q1/68
CPC分类号： C12N15/8261 , C12N15/1051 , C12N15/82 , C12N15/8216
摘要： The invention relates to efficient, high-throughput methods, systems, and DNA constructs for identification and isolation of transcription termination sequences. The invention relates further to specific terminator sequences identified by the methods isolated from rice.
摘要翻译：本发明涉及用于鉴定和分离转录终止序列的有效的高通量方法，系统和DNA构建体。本发明进一步涉及通过从水稻中分离的方法鉴定的具体终止子序列。

6. 再颁专利

USRE44750E1 Disarmed agrobacterium strains, ri-plasmids, and methods of transformation based thereon 有权
标题翻译：解除农杆菌菌株，ri质粒和其上的转化方法
公开(公告)号：USRE44750E1
公开(公告)日：2014-02-04
申请号：US13608451
申请日：2012-09-10
申请人： Luke Mankin , Dwight-Steven Hill , Paula Olhoft , Effie Toren , Jeffrey A. Brown , Liqun Xing , Huihua Fu , Lesley Ireland , Hongmei Jia , Hee-Sook Song , Allan R. Wenck , Larry Nea
发明人： Luke Mankin , Dwight-Steven Hill , Paula Olhoft , Effie Toren , Jeffrey A. Brown , Liqun Xing , Huihua Fu , Lesley Ireland , Hongmei Jia , Hee-Sook Song , Allan R. Wenck , Larry Nea
IPC分类号： C12N15/84 , C12N1/20 , C12N1/21 , A01H1/04 , C12N15/31
CPC分类号： C07K14/195 , C12N15/8205
摘要： The present invention relates “disarmed” strain variants of Agrobacterium strain K599 (NCPPB 2659), “disarmed” plasmid variants of the Ri-plasmid pRi2659, and derivatives thereof, and methods employing these strains and plasmids in plant transformation.
摘要翻译：本发明涉及农杆菌菌株K599（NCPPB2659）的“撤防”菌株变体，Ri质粒pRi2659的“解除武装”质粒变体及其衍生物，以及在植物转化中使用这些菌株和质粒的方法。

7. 发明授权

US10519458B2 Whole seed specific promoter 有权
公开(公告)号：US10519458B2
公开(公告)日：2019-12-31
申请号：US13265205
申请日：2010-04-22
申请人： Huihua Fu , Jeffrey A. Brown , Kirk Francis , Hee-Sook Song
发明人： Huihua Fu , Jeffrey A. Brown , Kirk Francis , Hee-Sook Song
IPC分类号： C12N15/82 , C12N15/113
摘要： The present invention is concerned with the provision of means and methods for gene expression. Specifically, it relates to a polynucleotide comprising an expression control sequence which allows for seed specific of a nucleic acid of interest being operatively linked thereto in plants. Furthermore, vectors, host cells, transgenic plants and methods for expressing nucleic acids of interest are provided which are based on the said polynucleotide.

8. 发明申请

US20100162428A1 Method Of Excising A Nucleic Acid Sequence From A Plant Genome 审中-公开
标题翻译：从植物基因组中切除核酸序列的方法
公开(公告)号：US20100162428A1
公开(公告)日：2010-06-24
申请号：US12600902
申请日：2008-05-30
申请人： Jeffrey A. Brown , Fang-Ming Lai , Christina E. Roche , Hee-Sook Song
发明人： Jeffrey A. Brown , Fang-Ming Lai , Christina E. Roche , Hee-Sook Song
IPC分类号： A01H1/00 , A01H5/00
CPC分类号： C12N15/8201 , C12N9/22
摘要： The present invention relates to a method for excising a nucleic acid sequence from the genome of a plant or a plant cell. This method is based on the steps of transforming a plant cell with a construct encoding a DNA double strand break inducing enzyme (DSBI), generating a transgenic plant line, performing a transient assay to analyze the functionality of the transgenic enzyme, crossing the plant line with a line containing a nucleic acid sequence to be excised and performing an immature embryo conversion or a tissue culture regeneration through callus formation. The method can also be reversed, which means that a plant cell is transformed with a construct encoding a nucleic acid sequence to be excised, and the crossing is performed with a plant line containing a DSBI. As an alternative to the crossing step, a re-transformation of a transgenic plant line with a second construct can also be performed. The invention is also directed to a plant obtained by this method, or progeny, propagation material, part, tissue, cell or cell culture, derived from such a plant. Finally, the invention relates to the use of a plant or progeny, propagation material, part, tissue, cell or cell culture, derived from this method, as aliment, fodder or seeds or for the production of pharmaceuticals or chemicals.
摘要翻译：本发明涉及从植物或植物细胞的基因组中切除核酸序列的方法。该方法基于用编码DNA双链断裂诱导酶（DSBI）的构建体转化植物细胞的步骤，产生转基因植物系，进行瞬时测定以分析转基因酶的功能，穿过植物系含有要切除的核酸序列的线，并通过愈伤组织形成进行未成熟胚转化或组织培养再生。该方法也可以相反，这意味着用编码待切除的核酸序列的构建体转化植物细胞，并且用含有DSBI的植物系进行杂交。作为交叉步骤的替代方案，也可以进行具有第二构建体的转基因植物系的再转化。本发明还涉及通过该方法获得的植物，或从这种植物衍生的后代，繁殖材料，部分，组织，细胞或细胞培养物。最后，本发明涉及来源于该方法的植物或后代，繁殖材料，部分，组织，细胞或细胞培养物作为营养物，饲料或种子或用于生产药物或化学品的用途。

9. 发明申请

US20120117688A1 Expression Cassettes for Endosperm-Specific Expression in Plants 有权
标题翻译：用于植物中胚乳特异性表达的表达盒
公开(公告)号：US20120117688A1
公开(公告)日：2012-05-10
申请号：US13383112
申请日：2010-07-06
申请人： Huihua Fu , Jeffrey A. Brown , Kirk Francis , Hee-Sook Song
发明人： Huihua Fu , Jeffrey A. Brown , Kirk Francis , Hee-Sook Song
IPC分类号： A01H5/00 , A01H1/06 , A01H5/10 , C12N15/82 , C12N5/10
CPC分类号： C07K14/415 , C12N15/8234
摘要： The present invention relates to an expression cassette for regulating seed-specific expression of a polynucleotide of interest, said expression cassette comprising a transcription regulating nucleotide sequence, to a vector comprising said expression cassette, host cells and transgenic plants comprising the expression cassette, and methods of producing said transgenic plants.
摘要翻译：本发明涉及一种表达盒，其用于将包含转录调节核苷酸序列的所述目的多核苷酸的种子特异性表达调控到包含所述表达盒的载体，宿主细胞和包含表达盒的转基因植物和方法生产所述转基因植物。

10. 发明申请

US20120036595A1 Whole Seed Specific Promoter 审中-公开
标题翻译：全种子特异性启动子
公开(公告)号：US20120036595A1
公开(公告)日：2012-02-09
申请号：US13265205
申请日：2010-04-22
申请人： Huihua Fu , Jeffrey A. Brown , Kirk Francis , Hee-Sook Song
发明人： Huihua Fu , Jeffrey A. Brown , Kirk Francis , Hee-Sook Song
IPC分类号： A01H1/06 , C12N15/82 , C12N5/04 , A01H5/00 , A01H5/10 , C12N15/113 , C12N5/10
CPC分类号： C12N15/8234 , C12N15/113
摘要： The present invention is concerned with the provision of means and methods for gene expression. Specifically, it relates to a polynucleotide comprising an expression control sequence which allows for seed specific of a nucleic acid of interest being operatively linked thereto in plants. Furthermore, vectors, host cells, transgenic plants and methods for expressing nucleic acids of interest are provided which are based on the said polynucleotide.
摘要翻译：本发明涉及提供基因表达的方法和方法。具体地，本发明涉及包含允许在植物中可操作地连接到其上的感兴趣的核酸的种子特异性的表达控制序列的多核苷酸。此外，提供了基于所述多核苷酸的载体，宿主细胞，转基因植物和表达目的核酸的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式