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1. 发明申请

US20070292953A1 Disarmed Agrobacterium Strains, Ri-Plasmids, and Methods of Transformation Based Thereon 有权
标题翻译：解除农杆菌菌株，Ri质粒及其转化方法
公开(公告)号：US20070292953A1
公开(公告)日：2007-12-20
申请号：US11661703
申请日：2005-08-31
申请人： Luke Mankin , Dwight-Steven Hill , Paula Olhoft , Effie Toren , Jeffrey Brown , Liqun Xing , Huihua Fu , Lesley Ireland , Hongmei Jia , Hee-Sook Song , Allan Wenck , Larry Nea
发明人： Luke Mankin , Dwight-Steven Hill , Paula Olhoft , Effie Toren , Jeffrey Brown , Liqun Xing , Huihua Fu , Lesley Ireland , Hongmei Jia , Hee-Sook Song , Allan Wenck , Larry Nea
IPC分类号： C12N15/82 , A01K67/00 , C07K14/00 , C12N1/00 , C12N5/04 , C12N5/00 , C12N1/21 , C12N15/11
CPC分类号： C07K14/195 , C12N15/8205
摘要： The present invention relates “disarmed” strain variants of Agrobacterium strain K599 (NCPPB 2659), “disarmed” plasmid variants of the Ri-plasmid pRi2659, and derivatives thereof, and methods employing these strains and plasmids in plant transformation.
摘要翻译：本发明涉及农杆菌菌株K599（NCPPB2659）的“撤防”菌株变体，Ri质粒pRi2659的“解除武装”质粒变体及其衍生物，以及在植物转化中使用这些菌株和质粒的方法。

2. 发明申请

US20070243543A1 Method for Isolation of Transcription Termination Sequences 失效
标题翻译：转录终止序列的分离方法
公开(公告)号：US20070243543A1
公开(公告)日：2007-10-18
申请号：US11659126
申请日：2005-07-30
申请人： Hee-Sook Song , Michael Kock , Jeffrey Brown , Linda Loyall , Liqun Xing , Hongmei Jia , John McMillan , Lesley Ireland
发明人： Hee-Sook Song , Michael Kock , Jeffrey Brown , Linda Loyall , Liqun Xing , Hongmei Jia , John McMillan , Lesley Ireland
IPC分类号： C12Q1/68 , C07H21/04 , C12N15/82
CPC分类号： C12N15/8261 , C12N15/1051 , C12N15/82 , C12N15/8216
摘要： The invention relates to efficient, high-throughput methods, systems, and DNA constructs for identification and isolation of transcription termination sequences. The invention relates further to specific terminator sequences identified by said methods isolated from rice.
摘要翻译：本发明涉及用于鉴定和分离转录终止序列的有效的高通量方法，系统和DNA构建体。本发明进一步涉及通过从水稻中分离的所述方法鉴定的特异性终止子序列。

3. 发明授权

US08465915B2 Method for isolation of transcription termination sequences 失效
标题翻译：分离转录终止序列的方法
公开(公告)号：US08465915B2
公开(公告)日：2013-06-18
申请号：US11659126
申请日：2005-07-30
申请人： Hee-Sook Song , Michael Kock , Jeffrey A. Brown , Linda Patricia Loyall , Liqun Xing , Hongmei Jia , John McMillan , Lesley Ireland
发明人： Hee-Sook Song , Michael Kock , Jeffrey A. Brown , Linda Patricia Loyall , Liqun Xing , Hongmei Jia , John McMillan , Lesley Ireland
IPC分类号： C12Q1/68
CPC分类号： C12N15/8261 , C12N15/1051 , C12N15/82 , C12N15/8216
摘要： The invention relates to efficient, high-throughput methods, systems, and DNA constructs for identification and isolation of transcription termination sequences. The invention relates further to specific terminator sequences identified by the methods isolated from rice.
摘要翻译：本发明涉及用于鉴定和分离转录终止序列的有效的高通量方法，系统和DNA构建体。本发明进一步涉及通过从水稻中分离的方法鉴定的具体终止子序列。

4. 发明申请

US20120054922A1 EXPRESSION ENHANCING INTRON SEQUENCES 失效
标题翻译：表达增强内容序列
公开(公告)号：US20120054922A1
公开(公告)日：2012-03-01
申请号：US13241493
申请日：2011-09-23
申请人： Hee-Sook Song , Christian Dammann , Marc Morra , Jeffrey A. Brown , Liqun Xing , Hongmei Jia
发明人： Hee-Sook Song , Christian Dammann , Marc Morra , Jeffrey A. Brown , Liqun Xing , Hongmei Jia
IPC分类号： C40B30/04 , C12N1/21 , C40B30/02 , C12N1/19 , C12N5/10 , A01H5/00 , C12N15/63 , C12N1/15
CPC分类号： C12N15/8241 , C12N15/8216 , C12N15/8225 , C12N15/8227 , C12N15/8234
摘要： The invention relates to methods for the identification and use of introns with gene expression enhancing properties. The teaching of this invention enables the identification of introns causing intron-mediated enhancement (IME) of gene expression. The invention furthermore relates to recombinant expression construct and vectors comprising said IME-introns operably linked with a promoter sequence and a nucleic acid sequence. The present invention also relates to transgenic plants and plant cells transformed with these recombinant expression constructs or vectors, to cultures, parts or propagation material derived there from, and to the use of same for the preparation of foodstuffs, animal feeds, seed, pharmaceuticals or fine chemicals, to improve plant biomass, yield, or provide desirable phenotypes.
摘要翻译：本发明涉及鉴定和使用具有基因表达增强特性的内含子的方法。本发明的教导能够鉴定引起基因表达的内含子介导的增强（IME）的内含子。本发明还涉及重组表达构建体和包含与启动子序列和核酸序列可操作地连接的所述IME-内含子的载体。本发明还涉及用这些重组表达构建体或载体转化的转基因植物和植物细胞，从其衍生的培养物，部分或繁殖材料，以及其用于制备食品，动物饲料，种子，药物或精细化学品，以改善植物生物量，产量或提供期望的表型。

5. 发明授权

US08088971B2 Expression enhancing intron sequences 有权
标题翻译：表达增强内含子序列
公开(公告)号：US08088971B2
公开(公告)日：2012-01-03
申请号：US11885988
申请日：2006-03-07
申请人： Hee-Sook Song , Marc Morra , Liqun Xing , Hongmei Jia
发明人： Hee-Sook Song , Marc Morra , Liqun Xing , Hongmei Jia
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8241 , C12N15/8216 , C12N15/8225 , C12N15/8227 , C12N15/8234
摘要： The invention relates to methods for the identification and use of introns with gene expression enhancing properties. The teaching of this invention enables the identification of introns causing intron-mediated enhancement (IME) of gene expression. The invention furthermore relates to recombinant expression construct and vectors comprising said IME-introns operably linked with a promoter sequence and a nucleic acid sequence. The present invention also relates to transgenic plants and plant cells transformed with these recombinant expression constructs or vectors, to cultures, parts or propagation material derived there from, and to the use of same for the preparation of foodstuffs, animal feeds, seed, pharmaceuticals or fine chemicals, to improve plant biomass, yield, or provide desirable phenotypes.
摘要翻译：本发明涉及鉴定和使用具有基因表达增强特性的内含子的方法。本发明的教导能够鉴定引起基因表达的内含子介导的增强（IME）的内含子。本发明还涉及重组表达构建体和包含与启动子序列和核酸序列可操作地连接的所述IME-内含子的载体。本发明还涉及用这些重组表达构建体或载体转化的转基因植物和植物细胞，从其衍生的培养物，部分或繁殖材料，以及其用于制备食品，动物饲料，种子，药物或精细化学品，以改善植物生物量，产量或提供期望的表型。

6. 发明授权

US07989678B2 Disarmed agrobacterium strains, Ri-plasmids, and methods of transformation based thereon 有权
标题翻译：解除农杆菌菌株，Ri质粒和其上的转化方法
公开(公告)号：US07989678B2
公开(公告)日：2011-08-02
申请号：US11661703
申请日：2005-08-31
申请人： Luke Mankin , Dwight-Steven Hill , Paula Olhoft , Effie Toren , Jeffrey A. Brown , Liqun Xing , Huihua Fu , Lesley Ireland , Hongmei Jia , Hee-Sook Song , Allan Richard Wenck , Larry Nea
发明人： Luke Mankin , Dwight-Steven Hill , Paula Olhoft , Effie Toren , Jeffrey A. Brown , Liqun Xing , Huihua Fu , Lesley Ireland , Hongmei Jia , Hee-Sook Song , Allan Richard Wenck , Larry Nea
IPC分类号： C12N15/84 , C12N1/20 , C12N1/21 , C12N15/31
CPC分类号： C07K14/195 , C12N15/8205
摘要： The present invention relates “disarmed” strain variants of Agrobacterium strain K599 (NCPPB 2659), “disarmed” plasmid variants of the Ri-plasmid pRi2659, and derivatives thereof, and methods employing these strains and plasmids in plant transformation.
摘要翻译：本发明涉及农杆菌菌株K599（NCPPB2659）的“撤防”菌株变体，Ri质粒pRi2659的“解除武装”质粒变体及其衍生物，以及在植物转化中使用这些菌株和质粒的方法。

7. 发明授权

US08759506B2 Expression enhancing intron sequences 失效
标题翻译：表达增强内含子序列
公开(公告)号：US08759506B2
公开(公告)日：2014-06-24
申请号：US13241493
申请日：2011-09-23
申请人： Hee-Sook Song , Christian Dammann , Marc Morra , Jeffrey A. Brown , Liqun Xing , Hongmei Jia
发明人： Hee-Sook Song , Christian Dammann , Marc Morra , Jeffrey A. Brown , Liqun Xing , Hongmei Jia
IPC分类号： A01H5/00 , C07H21/04 , C12N15/113 , C12N15/63 , C12N15/82 , C12N1/21
CPC分类号： C12N15/8241 , C12N15/8216 , C12N15/8225 , C12N15/8227 , C12N15/8234
摘要： The invention relates to methods for the identification and use of introns with gene expression enhancing properties. The teaching of this invention enables the identification of introns causing intron-mediated enhancement (IME) of gene expression. The invention furthermore relates to recombinant expression construct and vectors comprising said IME-introns operably linked with a promoter sequence and a nucleic acid sequence. The present invention also relates to transgenic plants and plant cells transformed with these recombinant expression constructs or vectors, to cultures, parts or propagation material derived there from, and to the use of same for the preparation of foodstuffs, animal feeds, seed, pharmaceuticals or fine chemicals, to improve plant biomass, yield, or provide desirable phenotypes.
摘要翻译：本发明涉及鉴定和使用具有基因表达增强特性的内含子的方法。本发明的教导能够鉴定引起基因表达的内含子介导的增强（IME）的内含子。本发明还涉及重组表达构建体和包含与启动子序列和核酸序列可操作地连接的所述IME-内含子的载体。本发明还涉及用这些重组表达构建体或载体转化的转基因植物和植物细胞，从其衍生的培养物，部分或繁殖材料，以及其用于制备食品，动物饲料，种子，药物或精细化学品，以改善植物生物量，产量或提供期望的表型。

8. 再颁专利

USRE44750E1 Disarmed agrobacterium strains, ri-plasmids, and methods of transformation based thereon 有权
标题翻译：解除农杆菌菌株，ri质粒和其上的转化方法
公开(公告)号：USRE44750E1
公开(公告)日：2014-02-04
申请号：US13608451
申请日：2012-09-10
申请人： Luke Mankin , Dwight-Steven Hill , Paula Olhoft , Effie Toren , Jeffrey A. Brown , Liqun Xing , Huihua Fu , Lesley Ireland , Hongmei Jia , Hee-Sook Song , Allan R. Wenck , Larry Nea
发明人： Luke Mankin , Dwight-Steven Hill , Paula Olhoft , Effie Toren , Jeffrey A. Brown , Liqun Xing , Huihua Fu , Lesley Ireland , Hongmei Jia , Hee-Sook Song , Allan R. Wenck , Larry Nea
IPC分类号： C12N15/84 , C12N1/20 , C12N1/21 , A01H1/04 , C12N15/31
CPC分类号： C07K14/195 , C12N15/8205
摘要： The present invention relates “disarmed” strain variants of Agrobacterium strain K599 (NCPPB 2659), “disarmed” plasmid variants of the Ri-plasmid pRi2659, and derivatives thereof, and methods employing these strains and plasmids in plant transformation.
摘要翻译：本发明涉及农杆菌菌株K599（NCPPB2659）的“撤防”菌株变体，Ri质粒pRi2659的“解除武装”质粒变体及其衍生物，以及在植物转化中使用这些菌株和质粒的方法。

9. 发明申请

US20090144863A1 Expression Enhancing Intron Sequences 有权
标题翻译：表达增强内含子序列
公开(公告)号：US20090144863A1
公开(公告)日：2009-06-04
申请号：US11885988
申请日：2006-03-07
申请人： Hee-Sook Song , Christian Dammann , Marc Morra , Jeffrey A. Brown , Liqun Xing , Hongmei Jia
发明人： Hee-Sook Song , Christian Dammann , Marc Morra , Jeffrey A. Brown , Liqun Xing , Hongmei Jia
IPC分类号： A01H5/00 , C12Q1/68 , C40B30/00 , C12N15/00 , C12N15/11 , C12N5/04
CPC分类号： C12N15/8241 , C12N15/8216 , C12N15/8225 , C12N15/8227 , C12N15/8234
摘要： The invention relates to methods for the identification and use of introns with gene expression enhancing properties. The teaching of this invention enables the identification of introns causing intron-mediated enhancement (IME) of gene expression. The invention furthermore relates to recombinant expression construct and vectors comprising said IME-introns operably linked with a promoter sequence and a nucleic acid sequence. The present invention also relates to transgenic plants and plant cells transformed with these recombinant expression constructs or vectors, to cultures, parts or propagation material derived there from, and to the use of same for the preparation of foodstuffs, animal feeds, seed, pharmaceuticals or fine chemicals, to improve plant biomass, yield, or provide desirable phenotypes.
摘要翻译：本发明涉及鉴定和使用具有基因表达增强特性的内含子的方法。本发明的教导能够鉴定引起基因表达的内含子介导的增强（IME）的内含子。本发明还涉及重组表达构建体和包含与启动子序列和核酸序列可操作地连接的所述IME-内含子的载体。本发明还涉及用这些重组表达构建体或载体转化的转基因植物和植物细胞，从其衍生的培养物，部分或繁殖材料，以及其用于制备食品，动物饲料，种子，药物或精细化学品，以改善植物生物量，产量或提供期望的表型。

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