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    • 2. 发明申请
    • LUCIFERIN-LUCIFERASE BASED MICRODEVICE FOR BIOSENSING
    • LUCIFERIN-LUCIFERASE基于微生物鉴定的MICRODEVICE
    • WO2009129646A1
    • 2009-10-29
    • PCT/CN2008/000811
    • 2008-04-21
    • HONEYWELL INTERNATIONAL INC.PAN, TaoSUN, ZhenhongWANG, WendyLIU, Xuanbin
    • PAN, TaoSUN, ZhenhongWANG, WendyLIU, Xuanbin
    • G01N21/76
    • G01N21/763
    • A method and apparatus for determining the concentration of one or more microbes in a sample is provided. This method involves filtering a sample through a filter inside a sample tube to retain the one or more microbes on the filter. The resulting filtrate, which contains or produces adenosine triphosphate, is passed through the sample tube and enters the reporter region. In the reporter region, the adenosine triphosphate in the filtrate comes in contact with a transparent porous matrix, which includes a luciferin-luciferase complex. The adenosine triphosphate interacts with the luciferin-luciferase complex to provide light response, which is measured by a detector. The light response is compared with a calibration curve to determine the total concentration of one or more microbes in a sample.
    • 提供了用于确定样品中一种或多种微生物浓度的方法和装置。 该方法包括通过样品管内的过滤器过滤样品以将一种或多种微生物保留在过滤器上。 将含有或产生三磷酸腺苷的所得滤液通过样品管并进入报道区。 在报道区域中,滤液中的三磷酸腺苷与透明的多孔基质接触,其包括荧光素 - 荧光素酶复合物。 三磷酸腺苷与荧光素 - 荧光素酶复合物相互作用以提供光响应,其由检测器测量。 将光响应与校准曲线进行比较,以确定样品中一种或多种微生物的总浓度。
    • 3. 发明申请
    • MICROARRAY READER BASED ON EVANESCENT WAVE DETECTION
    • 基于历史波检测的微型读取器
    • WO2011143791A1
    • 2011-11-24
    • PCT/CN2010/000709
    • 2010-05-20
    • HONEYWELL INTERNATIONAL INC.LIU, XuanbinPAN, TaoSUN, Zhenhong
    • LIU, XuanbinPAN, TaoSUN, Zhenhong
    • G01N21/64G01N33/53C12Q1/68
    • G01N21/64C12Q1/686G01N21/6452G01N21/648
    • A microarray reader (500) includes a light source (501) which emits light and beam shaping elements (502) positioned near the light source (501) to direct the light. The microarray reader (500) further includes a microarray (504) that is at least formed of an optical substrate (505) and a reaction chamber (506) in contact with the optical substrate (505). A buffer solution (507) is encapsulated by the optical substrate (505) and the reaction chamber (506) and a holder (508) aligns the microarray (504) relative to the light source (501) such that when light hits the buffer solution (507) molecules in the buffer solution (507) are excited to emit fluorescent light. A temperature control component (509) is thermally engaged with the holder (508) such that the temperature control component (509) adjusts the temperature of the buffer solution (507). The microarray reader (500) further includes a sensor (510) that receives the fluorescent light emitted by the excited molecules in the buffer solution (507) to generate a signal. The microarray reader (500) provides a simple and low-cost solution to overcome the drawback of improper alignment of the microarray (504) on the temperature control component (509).
    • 微阵列读取器(500)包括发射光的光源(501)和位于光源(501)附近的引导光的光束成形元件(502)。 微阵列读取器(500)还包括微阵列(504),其至少由与光学基板(505)接触的光学基板(505)和反应室(506)形成。 缓冲溶液(507)由光学基底(505)和反应室(506)封装,并且保持器(508)相对于光源(501)对准微阵列(504),使得当光照射缓冲溶液 缓冲溶液(507)中的(507)分子被激发以发射荧光。 温度控制部件(509)与保持器(508)热接合,使得温度控制部件(509)调节缓冲溶液(507)的温度。 微阵列读取器(500)还包括传感器(510),其接收由缓冲溶液(507)中的激发分子发射的荧光以产生信号。 微阵列读取器(500)提供了简单且低成本的解决方案,以克服微阵列(504)在温度控制部件(509)上的不正确对准的缺点。