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1. 发明专利

JPH06253900A REAGENT FOR EXAMINATION OF VIRUS AND EXAMINATION USING THE SAME REAGENT 失效
公开(公告)号：JPH06253900A
公开(公告)日：1994-09-13
申请号：JP4895393
申请日：1993-03-10
申请人： HITACHI LTD , B M L KK
发明人： MURAKAWA KATSUJI , KANBARA HIDEKI , NAGAI KEIICHI , FURUYAMA HIROKO , SUGIURA MASAHIKO , YAMAGUCHI TOSHIKAZU
IPC分类号： C12Q1/70 , C12N15/09 , C12Q1/68 , G01N33/50
摘要： PURPOSE:To provide a reagent for examination of virus, capable of readily detecting plural kinds of Herpes viruses using a pair of primers at a low cost and to provide an examination method using the reagent. CONSTITUTION:There is provided a reagent for examination of virus, containing an oligo nucleotide having a base sequence at least partly composed of a common base sequence derived from a gene coding the same kind of protein in plural different kinds of virus genomes. The common base sequence is derived from a gene coding DNA polymerase in the plural different kinds of virus genomes and one or more of the plural different kinds of viruses are herpes simplex virus 1 type, 2 type, human cytomegalovirus, Epstein-Barr virus or varicella-zoster virus. A pair of primers each having a base sequence common to plural kinds of Herpes viruses and forming elongation reaction products mutually different in its length are used to amplify DNA and the products are detected. The number of times of reaction operations required for the examination can be reduced.

2. 发明专利

JPH06261800A DETECTION OF RNA VIRUS 失效
公开(公告)号：JPH06261800A
公开(公告)日：1994-09-20
申请号：JP4895493
申请日：1993-03-10
申请人： HITACHI LTD , B M L KK
发明人： NAGAI KEIICHI , KANBARA HIDEKI , MURAKAWA KATSUJI , OKANO KAZUNOBU , YAMAGUCHI TOSHIKAZU , SUGIURA MASAHIKO
IPC分类号： C12Q1/70
摘要： PURPOSE:To provide a detection process for RNA virus suitable for automation. CONSTITUTION:In a single-pot manner, the sample serum is introduced into a reaction vessel containing the particles to which a single strand DNA 3 having a base sequence complementary to a specific part of RNA 2 in the target RNA virus is immobilized to effect the RNA extraction reaction and immobilize the freed RNA by the hybridization reaction. After the vessel is rinsed, a solution containing transcriptase, a heat-resistant DNA polymerase and an amplification marker labeled with a fluorescent pigment 8 is added to the vessel, whose the temperature is kept at a prescribed temperature to effect the synthesis of cDNA 6 and the amplification of single-stranded DNA labeled with a fluorescent pigment. After the unreacting primer is excluded, a single-stranded DNA is isolated from the particles 11, transferred into a cell for the fluorescent detection 12 to measure the fluorescence to determine the amplified DNA, namely the target virus.

3. 发明专利

JPH06153996A METHOD FOR DETECTING POLYNUCLEOTIDE 失效
公开(公告)号：JPH06153996A
公开(公告)日：1994-06-03
申请号：JP30903792
申请日：1992-11-18
申请人： HITACHI LTD , B M L KK
发明人： OKANO KAZUNOBU , MURAKAWA KATSUJI , NAGAI KEIICHI , KANBARA HIDEKI , SUGIURA MASAHIKO , KATAYAMA KAZUHIKO
IPC分类号： C12Q1/68 , C12Q1/70 , G01N33/50 , G01N33/53 , G01N33/58 , G01N37/00
摘要： PURPOSE:To determine a polynucleotide to be measured in a sample in high sensitivity without requiring a radioactive isotope by labelling a target RNA with a marker material and catching the target RNA with a carrier to which a ribosome is immobilized. CONSTITUTION:A target RNA (RNA virus, mRNA, etc.) is labelled with a marker material and the labelled RNA is caught with a carrier to which ribosome is immobirized. Since the RNA is almost single strand it contains ribosome binding part at the 5' end or in its neighborhood.

4. 发明专利

JPH06261795A PROCESS FOR DETECTING POLYNUCLEOTIDES 失效
公开(公告)号：JPH06261795A
公开(公告)日：1994-09-20
申请号：JP4895593
申请日：1993-03-10
申请人： HITACHI LTD , B M L KK
发明人： OKANO KAZUNOBU , KANBARA HIDEKI , NAGAI KEIICHI , MURAKAWA KATSUJI , SUGIURA MASAHIKO , YAMAGUCHI TOSHIKAZU
IPC分类号： C12Q1/68
摘要： PURPOSE:To provide a quantitative process for detecting polynucleotides in high sensitivity and high accuracy. CONSTITUTION:Five kinds of fluorescent probes 2 to 6 to which two molecules of fluorescent compounds are bonded each, are allowed to react with the target nucleotide chain 1 under the hybridization conditions, the reaction mixture is added to the inlet 21 for the electrophoretic carrier to perform the electrophoresis, thereby the unreacting labeled probes of smaller molecular sizes are removed. The complex of the fluorescent probe with the target polynucleotide chain 10 is a large size of molecule and remains near the inlet to be concentrated. The inlet part is heated to dissolve the fluorescent probe from the target polynucleotide and the probe crosses the a certain zone illuminated with a laser beam in the electrophoretic column to detect the fluorescence. The target polynucleotide of 100 copy order can be detected.

5. 发明专利

GB2143673B IONIZING SAMPLES FOR SECONDARY ION MASS SPECTROMETRY 未知
公开(公告)号：GB2143673B
公开(公告)日：1987-11-11
申请号：GB8415193
申请日：1984-06-14
申请人： HITACHI LTD
发明人： SAKAIRI MINORU , KANBARA HIDEKI
IPC分类号： G01N23/225 , G01N27/62 , H01J37/252 , H01J49/02 , H01J49/10 , H01J49/14

6. 发明专利

GB2143673A Ionizing samples for secondary ion mass spectrometry 未知
公开(公告)号：GB2143673A
公开(公告)日：1985-02-13
申请号：GB8415193
申请日：1984-06-14
申请人： HITACHI LTD
发明人： SAKAIRI MINORU , KANBARA HIDEKI
IPC分类号： G01N23/225 , G01N27/62 , H01J37/252 , H01J49/02 , H01J49/10 , H01J49/14
摘要： An interface for coupling a secondary ion mass spectrometer to a liquid chromatograph for separating an organic sample in solution, comprises a particle beam generation section 38 for generating a primary particle beam 36, and a sample ionization chamber 20 having a target 42 for depositing the sample inside the chamber, the target being subject to bombardment by the primary particle beam 36 in an inclined direction, wherein a molecular beam-like sample inlet section 14 is so structured as to introduce a solution of the organic sample in a molecular beam-like state 16 into the ionization chamber and to allow the solution to hit the position to be bombarded by the primary particle beam on the surface of the target, whereby a secondary ion beam 40 is generated from the organic sample. The target may be a movable belt or a plate. The inlet section 14 includes a differential pumping system for reaching the vacuum of the ionization chamber in stages.

7. 发明专利

JP2013116081A Method for detecting pyrophosphoric acid using boronic acid group-immobilized support 有权
标题翻译：使用硼酸组固定支持检测磷酸的方法
公开(公告)号：JP2013116081A
公开(公告)日：2013-06-13
申请号：JP2011265660
申请日：2011-12-05
申请人： Hitachi Ltd , 株式会社日立製作所
发明人： NISHIDA YOICHI , KANBARA HIDEKI
IPC分类号： C12Q1/68 , C12N15/09 , C12Q1/48 , G01N27/414 , G01N27/416 , G01N33/53
CPC分类号： C12Q1/6811 , C12N15/09 , C12Q1/68 , C12Q1/6869 , G01N27/4145 , C12Q2527/125 , C12Q2565/301
摘要： PROBLEM TO BE SOLVED: To provide a method for easily and directly determining DNA sequence by an enzyme reaction, especially directly detecting a pyrophosphoric acid discharged by a DNA polymerase or a DNA ligase.SOLUTION: There is provided the method and a device for detecting the pyrophosphoric acid, in which a boronic group immobilized on a conductive support is reversibly bonded to the pyrophosphoric acid, by measuring an electric change induced at that time.
摘要翻译：待解决的问题：提供通过酶反应容易且直接测定DNA序列的方法，特别是直接检测由DNA聚合酶或DNA连接酶排出的焦磷酸。提供了通过测量此时引起的电变化的方法和用于检测焦磷酸的方法和装置，其中将固定在导电载体上的硼基可逆地结合到焦磷酸上。版权所有（C）2013，JPO＆INPIT

8. 发明专利

JP2011203263A Device for analysis of small gene 有权
标题翻译：小基因分析装置
公开(公告)号：JP2011203263A
公开(公告)日：2011-10-13
申请号：JP2011117937
申请日：2011-05-26
申请人： Hitachi Ltd , 株式会社日立製作所
发明人： KAJIYAMA TOMOHARU , KANBARA HIDEKI , HARADA KUNIO
IPC分类号： G01N35/10 , C12M1/00 , C12M1/34 , C12N15/00 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To solve the problem with a conventional technique of dispensing a variety of reagents with high accuracy, wherein the technique has too complicated mechanism to easily achieve miniaturization and low cost.SOLUTION: A small, simple and low cost analysis device is achieved by preparing air layer on the end of a capillary after dispensation in order to achieve a pressure dispensing method using the capillary with a plurality of reagents and further reduce leakage of any reagent other than the dispensing reagent.
摘要翻译：要解决的问题：为了解决以高精度分配各种试剂的现有技术的问题，其中该技术具有太复杂的机构以容易实现小型化和低成本。解决方案：一种小型，简单和低成本的分析装置通过在分配后在毛细管末端制备空气层来实现，以便实现使用具有多种试剂的毛细管的压力分配方法，并且进一步减少除了分配试剂之外的任何试剂的泄漏。

9. 发明专利

JP2009002806A Chemiluminescence measuring device 审中-公开
标题翻译：亮度测量装置
公开(公告)号：JP2009002806A
公开(公告)日：2009-01-08
申请号：JP2007164175
申请日：2007-06-21
申请人： Hitachi Ltd , 株式会社日立製作所
发明人： SHIRAI MASATAKA , KAJIYAMA TOMOHARU , KANBARA HIDEKI
IPC分类号： G01N21/76 , C12M1/00 , C12M1/34 , G01N37/00
CPC分类号： G01N21/76 , B01J2219/00313 , B01J2219/00337 , B01J2219/0036 , B01J2219/00704 , B01L3/5025 , B01L3/50273 , B01L3/502738 , B01L3/50853 , B01L2200/0642 , B01L2300/041 , B01L2300/046 , B01L2300/0654 , B01L2300/0819 , B01L2300/0877 , B01L2300/1822 , B01L2300/1827 , G01N21/05 , G01N2021/0325 , G01N2021/0346 , G01N2021/036
摘要： PROBLEM TO BE SOLVED: To provide a chemiluminescence measuring device capable of performing quick supply of a reagent material into minute reaction vessels, and performing adjacent reaction analysis, or the like.
SOLUTION: This chemiluminescence measuring device comprises a plate, on which a plurality of reaction vessels are provided; a transparent substrate facing the plate, for forming a channel between itself and the plate; an injection part for injecting selectively a reaction substrate and a cleaning liquid into the channel; an imaging system for imaging through the transparent substrate, luminescence, caused by a chemical reaction between a reaction material held in the plurality of reaction vessels, provided on the plate and the reaction substrate supplied through the channel; and a channel control means for changing the conductance of the channel or the channel sectional shape. High-accuracy DNA analyses which use a step reaction can be realized in gene arrangement analysis, by deforming the shape of the channel in a flow cell 101 which includes the plate 201, on which the fine reaction vessels 103 are provided between a material supply time and a luminescent reaction time.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供一种化学发光测量装置，其能够将试剂材料快速供应到微小的反应容器中，并进行相邻的反应分析等。解决方案：该化学发光测量装置包括一个板，其上设有多个反应容器; 面对板的透明衬底，用于在其与板之间形成通道; 注入部，其用于将反应基板和清洗液选择性地注入到所述流路中; 用于通过透明基板成像的成像系统，通过设置在板上的反应材料和通过通道供给的反应基板之间的由多个反应容器中保持的反应材料之间的化学反应引起的发光; 以及用于改变通道的电导或通道截面形状的通道控制装置。使用阶段反应的高精度DNA分析可以在基因排列分析中实现，通过使包含板201的流动池101中的通道的形状变形，在其上设置有微细反应容器103的材料供给时间并发出发光反应时间。版权所有（C）2009，JPO＆INPIT

10. 发明专利

JP2008268069A Chemiluminescence detection device 有权
标题翻译：荧光检测装置
公开(公告)号：JP2008268069A
公开(公告)日：2008-11-06
申请号：JP2007113095
申请日：2007-04-23
申请人： Hitachi Ltd , 株式会社日立製作所
发明人： SHIRAI MASATAKA , KAJIYAMA TOMOHARU , KANBARA HIDEKI
IPC分类号： G01N21/76 , C12M1/00
CPC分类号： G01N21/76 , B01L3/502761 , B01L2200/0668 , B01L2300/0816 , B01L2300/0877 , G01N21/01 , G01N21/251 , G01N21/763
摘要： PROBLEM TO BE SOLVED: To improve a throughput by increasing the number of micro reaction tanks. SOLUTION: The chemiluminescence detection device includes many reaction tanks, having plates that are arranged one-dimensionally or two-dimensionally. In detecting light, a line or an area sensor having many detection pixels is used. An interval of light detection pixels approximately corresponds to an interval of the reaction tanks on the plate. The micro reaction tanks and pixels correspond, one by one on the plate so that light from the reaction tank is incident to the detection pixel most efficiently, so as not to disperse to other pixels. In order to make the micro reaction tanks and pixels of an imaging element arranged on the plate correspond one to one, a luminous body, reflector, or light absorber is placed on the plate as a positioning mark. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：通过增加微反应罐的数量来提高生产量。解决方案：化学发光检测装置包括许多反应罐，其具有一维或二维布置的板。在检测光时，使用具有许多检测像素的线或区域传感器。光检测像素的间隔大致对应于板上的反应罐的间隔。微反应池和像素在板上一个一个地对应，使得来自反应罐的光最有效地入射到检测像素，以便不分散到其他像素。为了使微反应槽和布置在板上的成像元件的像素一一对应，将发光体，反射体或光吸收体作为定位标记放置在板上。版权所有（C）2009，JPO＆INPIT

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