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1. 发明专利

DE60029913D1 未知
公开(公告)号：DE60029913D1
公开(公告)日：2006-09-21
申请号：DE60029913
申请日：2000-03-17
申请人： HITACHI LTD
发明人： KAJIYAMA TOMOHARU , MIYAHARA YUJI , MURAKAWA KATSUJI
IPC分类号： B01L7/00 , B01J19/00 , B01L3/00 , C12Q1/68 , C12Q1/6837 , C40B40/06 , C40B60/14

2. 发明专利

DE60025871D1 未知
公开(公告)号：DE60025871D1
公开(公告)日：2006-04-20
申请号：DE60025871
申请日：2000-10-13
申请人： HITACHI LTD , NAT CANCER CT TOKIO TOKYO
发明人： TOMITA HIROYUKI , MURAKAWA KATSUJI , MIYAHARA YUJI , MURAKAMI YOSHINORI , SEKIYA TAKAO
IPC分类号： C12N15/09 , C12Q1/68 , G01N21/78 , G01N27/447 , G01N33/50

3. 发明专利

DE60029913T2 未知
公开(公告)号：DE60029913T2
公开(公告)日：2007-09-06
申请号：DE60029913
申请日：2000-03-17
申请人： HITACHI LTD
发明人： KAJIYAMA TOMOHARU , MIYAHARA YUJI , MURAKAWA KATSUJI
IPC分类号： B01L7/00 , B01J19/00 , B01L3/00 , C12Q1/68 , C12Q1/6837 , C40B40/06 , C40B60/14

4. 发明专利

DE60025871T2 未知
公开(公告)号：DE60025871T2
公开(公告)日：2006-10-05
申请号：DE60025871
申请日：2000-10-13
申请人： HITACHI LTD , NAT CANCER CT
发明人： TOMITA HIROYUKI , MURAKAWA KATSUJI , MIYAHARA YUJI , MURAKAMI YOSHINORI , SEKIYA TAKAO
IPC分类号： C12N15/09 , C12Q1/68 , G01N21/78 , G01N27/447 , G01N33/50

5. 发明专利

JPH06261800A DETECTION OF RNA VIRUS 失效
公开(公告)号：JPH06261800A
公开(公告)日：1994-09-20
申请号：JP4895493
申请日：1993-03-10
申请人： HITACHI LTD , B M L KK
发明人： NAGAI KEIICHI , KANBARA HIDEKI , MURAKAWA KATSUJI , OKANO KAZUNOBU , YAMAGUCHI TOSHIKAZU , SUGIURA MASAHIKO
IPC分类号： C12Q1/70
摘要： PURPOSE:To provide a detection process for RNA virus suitable for automation. CONSTITUTION:In a single-pot manner, the sample serum is introduced into a reaction vessel containing the particles to which a single strand DNA 3 having a base sequence complementary to a specific part of RNA 2 in the target RNA virus is immobilized to effect the RNA extraction reaction and immobilize the freed RNA by the hybridization reaction. After the vessel is rinsed, a solution containing transcriptase, a heat-resistant DNA polymerase and an amplification marker labeled with a fluorescent pigment 8 is added to the vessel, whose the temperature is kept at a prescribed temperature to effect the synthesis of cDNA 6 and the amplification of single-stranded DNA labeled with a fluorescent pigment. After the unreacting primer is excluded, a single-stranded DNA is isolated from the particles 11, transferred into a cell for the fluorescent detection 12 to measure the fluorescence to determine the amplified DNA, namely the target virus.

6. 发明专利

JPH06153996A METHOD FOR DETECTING POLYNUCLEOTIDE 失效
公开(公告)号：JPH06153996A
公开(公告)日：1994-06-03
申请号：JP30903792
申请日：1992-11-18
申请人： HITACHI LTD , B M L KK
发明人： OKANO KAZUNOBU , MURAKAWA KATSUJI , NAGAI KEIICHI , KANBARA HIDEKI , SUGIURA MASAHIKO , KATAYAMA KAZUHIKO
IPC分类号： C12Q1/68 , C12Q1/70 , G01N33/50 , G01N33/53 , G01N33/58 , G01N37/00
摘要： PURPOSE:To determine a polynucleotide to be measured in a sample in high sensitivity without requiring a radioactive isotope by labelling a target RNA with a marker material and catching the target RNA with a carrier to which a ribosome is immobilized. CONSTITUTION:A target RNA (RNA virus, mRNA, etc.) is labelled with a marker material and the labelled RNA is caught with a carrier to which ribosome is immobirized. Since the RNA is almost single strand it contains ribosome binding part at the 5' end or in its neighborhood.

7. 发明专利

JPH05168500A DETERMINATION OF NUCLEIC ACID BASE SEQUENCE 失效
公开(公告)号：JPH05168500A
公开(公告)日：1993-07-02
申请号：JP34435491
申请日：1991-12-26
申请人： HITACHI LTD
发明人： NISHIKAWA TETSUO , KANBARA HIDEKI , MURAKAWA KATSUJI
IPC分类号： C12M1/00 , C12Q1/68 , G01N27/447 , G01N33/50 , G06F19/20 , G06F19/26 , G06Q10/04 , G16B45/00
摘要： PURPOSE:To extend the range of the detectable base length by analyzing the electrophoretic pattern of a nucleic acid fragment generated by the dideoxy method by using a specified method. CONSTITUTION:Based on peak spectrum 8 of a signal from a nucleic acid fragment generated by the dideoxy method, the number Np of the kinds of bases corresponding to the respective peaks and having a length mutually different by the length of one base is obtained. The area surrounded by the line 11 perpendicular to the bottom of a valley in a group of the peaks, the circular part line 12 of the peak and the base line 13 is measured and normalized by a normalization factor of each peak. The average area of a prescribed number of right and left peaks involving the aimed peak is calculated and the calculated value is multiplied by a prescribed factor to determine a threshold value. After excluding peak areas having a larger value than the threshold value, the average value is calculated again so as to determine the normalization factor. Even when separation of the peaks based on detection of the maximum value is impossible because of overlap between the peaks, the number of the kinds of DNA fragments constituting the respective peaks and having a length mutually different by the length of one base can be detected. Thereby, the base sequence can be determined.

8. 发明专利

JPH03164200A ANALYSIS OF GENE BY LIQUID HYBRIDIZATION 失效
公开(公告)号：JPH03164200A
公开(公告)日：1991-07-16
申请号：JP30316689
申请日：1989-11-24
申请人： HITACHI LTD
发明人： FUJITA MASAHIKO , KANBARA HIDEKI , MURAKAWA KATSUJI , NAGAI KEIICHI , SHIMADA TAMOTSU
IPC分类号： C12Q1/68
摘要： PURPOSE:To detect the presence of a specific base sequence and the mutation of base pair and to analyze a gene by adding a specific nucleoside triphosphate, a DNA fragment and a DNA polymerase to a specimen containing nucleic acid to synthesize a complemental strand DNA and analyzing the DNA by gel electrophoresis. CONSTITUTION:Four kinds of nucleoside triphosphates, a DNA fragment having a specific sequence and a DNA polymerase are added to a DNA specimen containing nucleic acid of an organism or virus and subjected to hybridization. The synthesized complemental strand DNA is analyzed by gel-electrophoresis to analyze the gene by the base length pattern of the DNA. In the above process, the hydridization is carried out by using a non-labeled oligonucleotide terminator in combination with a labeled oligonucleotide primer and a DNA sequence between both components is synthesized to enable the analysis of gene using liquid hydridization technique.

9. 发明专利

JPH03118462A ELECTROPHORESIS APPARATUS 失效
公开(公告)号：JPH03118462A
公开(公告)日：1991-05-21
申请号：JP25543789
申请日：1989-09-30
申请人： HITACHI LTD
发明人： MURAKAWA KATSUJI , FUJITA MASAHIKO , NAGAI KEIICHI , SHIMADA TAMOTSU , KANBARA HIDEKI
IPC分类号： G01N27/447 , G01N27/26
摘要： PURPOSE:To obtain excellent reproducibility by simple, quick operations by providing at least two molecular-weight partitioning films in the order from the film for larger molecular weight or in the reverse order, and partitioning electrophoresis paths. CONSTITUTION:A migrating tank 4 is divided into a first chamber 4a, a second chamber 4b and a third chamber 4c with molecular-weight partitioning films 5 and 6. The surfaces of the films 5 and 6 face the chambers 4a, 4b and 4c, respectively. The dividing molecular weight of the film 5 is larger than the molecular weight of intended molecules 8. That of the film 6 is smaller. When an electric field is applied through electrodes 2 and 3, the molecules in the chamber 4a are migrated in the direction 3 from the electrode 2 to the electrode 3. Only the molecules smaller than the partitioned molecular weight of the film 5 pass the film 5 and move into the chamber 4b. Only the molecules which are smaller than that of the film 6 move into the chamber 4c. As a result, the intended molecules 8 are isolated in the chamber 4b. The molecules 9 which are larger than the partitioned molecular weight is separated in the chamber 4a. The molecules 10 smaller than the molecular weight are separated in the chamber 4c. Thus, the intended molecules can be separated and separately collected simply and quickly.

10. 发明专利

JPH03105251A PREPARING APPARATUS OF SAMPLE 失效
公开(公告)号：JPH03105251A
公开(公告)日：1991-05-02
申请号：JP24200889
申请日：1989-09-20
申请人： HITACHI LTD
发明人： FUJITA MASAHIKO , KANBARA HIDEKI , MURAKAWA KATSUJI , NAGAI KEIICHI , SHIMADA TAMOTSU
IPC分类号： G01N33/48 , G01N33/50
摘要： PURPOSE:To reduce a burden of an operator in relation to an operation for preparation of a sample by providing at least a vessel provided with a molecular-weight separating film, a centrifuge bearing the vessel and applying a centrifugal acceleration to a liquid and a vessel conveyor conveying the vessel. CONSTITUTION:A vessel 18 with a molecular-weight separating film is constructed of the molecular-weight separating film 18a, a base 18b of this separating film 18a, an O ring 18c and vessel walls 18d and 18e, and a condensed liquid 18f is collected onto the side of 18d and a filtered liquid 18g onto the side of 18e by applying a centrifugal acceleration in a centrifuge 11. Between the base 18b and the vessel wall 18e a gap 18i is provided so that air can enter and leave freely and that a filtering operation can be conducted smoothly. By this molecular-weight separating film, a substance of a smaller molecular weight than a prescribed value in a solution is filtered and a substance of a larger molecular weight than a prescribed weight is left on the upper side of the film. In order to increase the speed of filtration, in addition, the centrifugal acceleration is applied to the vessel by the centrifuge 11. On the occasion, the vessel 18 is set on the centrifuge 11 and a processing is executed by using a vessel conveyor 14.

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