专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US4677060A Process for determining the pH value in the interior of a cell 失效
标题翻译：确定细胞内部pH值的方法
公开(公告)号：US4677060A
公开(公告)日：1987-06-30
申请号：US680078
申请日：1984-12-10
申请人： Gunter Valet , Gerhard Ruhenstroth-Bauer , Erich Wunsch , Luis Moroder
发明人： Gunter Valet , Gerhard Ruhenstroth-Bauer , Erich Wunsch , Luis Moroder
IPC分类号： G01N21/75 , C07C255/51 , C07C255/53 , C07C271/06 , C12Q1/00 , C12Q1/02 , C12Q1/37 , C12Q1/44 , G01N21/64 , G01N21/80 , G01N33/84 , C12Q1/38
CPC分类号： C07C255/00 , G01N33/84
摘要： A process for determining the pH value in the interior of a cell is descrd. This is accomplished by measurement of the emission of a fluorescent substance which is absorbed by the cell through incubation in a solution. The solution contains a compound of the said fluorescent substance; the fluorescent substance is separated from this compound in the cell by an enzyme present in the cell. The fluorescent substance selected is one such that the frequency of the emission maximum is dependent on the pH value. The compound of this fluorescent substance which is selected is one which is membrane penetrating. Measurement of the fluorescence is based on the principle of measurement of the frequency at which the emission maximum occurs. A fluorescent substance suitable for this purpose is 2,3-dicyano-1,4-hydroquinone. A suitable membrane penetrating compound is 1,4-diacetoxy-2,3-dicyanobenzene or 1,4-dibutyryloxy-2,3-dicyanobenzene or 1,4-di(-tert-butyloxycarbonyl-1-alanyloxy)-2,3-dicyanobenzene.
摘要翻译：描述了确定细胞内部的pH值的方法。这是通过测量通过在溶液中孵育而被细胞吸收的荧光物质的发射来实现的。该溶液含有所述荧光物质的化合物; 通过存在于细胞中的酶将荧光物质从细胞中的该化合物中分离出来。选择的荧光物质是这样的，使得发射最大值的频率取决于pH值。所选择的荧光物质的化合物是膜渗透的化合物。荧光的测量是基于测量发射最大值的频率的原理。适用于此目的的荧光物质是2,3-二氰基-1,4-对苯二酚。合适的膜渗透化合物是1,4-二乙酰氧基-2,3-二氰基苯或1,4-二丁酰氧基-2,3-二氰基苯或1,4-二（叔丁氧基羰基-1-烷氧基）二氰基苯。

2. 发明授权

US4515730A Compounds for determining the pH value in the interior of a cell 失效
标题翻译：用于测定细胞内部pH值的化合物
公开(公告)号：US4515730A
公开(公告)日：1985-05-07
申请号：US394784
申请日：1982-07-02
申请人： Gunter Valet , Gerhard Ruhenstroth-Bauer , Erich Wunsch , Luis Moroder
发明人： Gunter Valet , Gerhard Ruhenstroth-Bauer , Erich Wunsch , Luis Moroder
IPC分类号： G01N21/75 , C07C255/51 , C07C255/53 , C07C271/06 , C12Q1/00 , C12Q1/02 , C12Q1/37 , C12Q1/44 , G01N21/64 , G01N21/80 , G01N33/84 , C07C121/75
CPC分类号： C07C255/00 , G01N33/84
摘要： A process for determining the pH value in the interior of a cell is described. This is accomplished by measurement of the emission of a fluorescent substance which is absorbed by the cell through incubation in a solution. The solution contains a compound of the said fluorescent substance; the fluorescent substance is separated from this compound in the cell by an enzyme present in the cell. The fluorescent substance selected is one such that the frequency of the emission maximum is dependent on the pH value. The compound of this fluorescent substance which is selected is one which is membrane penetrating. Measurement of the fluorescence is based on the principle of measurement of the frequency at which the emission maximum occurs. A fluorescent substance suitable for this purpose is 2,3-dicyano-1,4-hydroquinone. A suitable membrane penetrating compound is 1,4-diacetoxy-2,3-dicyanobenzene or 1,4-dibutyryloxy-2,3-dicyanobenzene or 1,4-di(-tert-butyloxycarbonyl-1-alanyloxy)-2,3-dicyanobenzene.
摘要翻译：描述了确定细胞内部的pH值的方法。这是通过测量通过在溶液中孵育而被细胞吸收的荧光物质的发射来实现的。该溶液含有所述荧光物质的化合物; 通过存在于细胞中的酶将荧光物质从细胞中的该化合物中分离出来。选择的荧光物质是这样的，使得发射最大值的频率取决于pH值。所选择的荧光物质的化合物是膜渗透的化合物。荧光的测量是基于测量发射最大值的频率的原理。适用于此目的的荧光物质是2,3-二氰基-1,4-对苯二酚。合适的膜渗透化合物是1,4-二乙酰氧基-2,3-二氰基苯或1,4-二丁酰氧基-2,3-二氰基苯或1,4-二（叔丁氧基羰基-1-烷氧基）二氰基苯。

3. 发明授权

US4503148A Process for determining the pH value in the interior of a cell 失效
公开(公告)号：US4503148A
公开(公告)日：1985-03-05
申请号：US346094
申请日：1982-02-05
申请人： G/u/ nter Valet , Gerhard Ruhenstroth-Bauer , Erich W/u/ nsch , Luis Moroder
发明人： G/u/ nter Valet , Gerhard Ruhenstroth-Bauer , Erich W/u/ nsch , Luis Moroder
IPC分类号： G01N21/75 , C07C255/51 , C07C255/53 , C07C271/06 , C12Q1/00 , C12Q1/02 , C12Q1/37 , C12Q1/44 , G01N21/64 , G01N21/80 , G01N33/84 , C12Q1/38
CPC分类号： C07C255/00 , G01N33/84
摘要： A process for determining the pH value in the interior of a cell is described. This is accomplished by measurement of the emission of a fluorescent substance which is absorbed by the cell through incubation in a solution. The solution contains a compound of the said fluorescent substance; the fluorescent substance is separated from this compound in the cell by an enzyme present in the cell. The fluorescent substance selected is one such that the frequency of the emission maximum is dependent on the pH value. The compound of this fluorescent substance which is selected is one which is membrane penetrating. Measurement of the fluorescence is based on the principle of measurement of the frequency at which the emission maximum occurs. A fluorescent substance suitable for this purpose is 2,3-dicyano-1,4-hydroquinone. A suitable membrane penetrating compound is 1,4-diacetoxy-2,3-dicyanobenzene or 1,4-dibutyryloxy-2,3-dicyanobenzene or 1,4-di(-tert-butyloxycarbonyl-1-alanyloxy)-2,3-dicyanobenzene.

4. 发明授权

US4018662A Method and apparatus for simultaneous quantitative analysis of several constituents in a sample 失效
标题翻译：用于同时定量分析样品中几种成分的方法和装置
公开(公告)号：US4018662A
公开(公告)日：1977-04-19
申请号：US646391
申请日：1976-01-02
申请人： Gerhard Ruhenstroth-Bauer , Reiner Scherer
发明人： Gerhard Ruhenstroth-Bauer , Reiner Scherer
IPC分类号： G01N27/447 , G01N27/00 , G01N27/26
CPC分类号： G01N27/447
摘要： A method for simultaneous qualitative and quantitative analysis of antigensr antigen-effective substances in a sample provides placing the sample in a carrier medium through which it migrates toward a plurality of physically separate carrier strips. The medium on each strip contains a quantity of a specific ingredient, for example an antibody, which is expected to undergo a specific immunoreaction with one of the constituents in the sample, thereby producing a visible precipitate. The dimensions of the final precipitate then permit a direct measurement of the identified substance. A known concentration of a calibrating substance may be added to the sample to provide for calibration precipitates in the various strips. The apparatus for practicing the method includes a base plate on which the neutral, antibody-free carrier medium as well as the various strips containing media with antibodies or other immunoreactive ingredients are located. An electric field may be applied to aid in the migration of the sample in the medium.
摘要翻译：用于同时定性和定量分析样品中的抗原或抗原有效物质的方法提供将样品置于载体介质中，通过该载体介质向多个物理上分离的载体条移动。每个条带上的培养基含有一定量的特定成分，例如抗体，其预期与样品中的一种成分进行特异性免疫反应，从而产生可见的沉淀物。然后，最终沉淀物的尺寸允许直接测量所鉴定的物质。可以将已知浓度的校准物质添加到样品中以提供各种条中的校准沉淀。用于实施该方法的装置包括基板，其上定位有中性，无抗体的载体介质以及含有抗体或其它免疫反应性成分的培养基的各种条。可以施加电场以帮助样品在介质中的迁移。

5. 发明授权

US4341765A Drug for enhancing liver growth and method of preparing same 失效
标题翻译：用于增强肝脏生长的药物及其制备方法
公开(公告)号：US4341765A
公开(公告)日：1982-07-27
申请号：US130648
申请日：1980-03-17
申请人： Gerhard Ruhenstroth-Bauer , Michel Goldberg , Siegfried Silz , Wolfgang Strecker
发明人： Gerhard Ruhenstroth-Bauer , Michel Goldberg , Siegfried Silz , Wolfgang Strecker
IPC分类号： A61K35/16 , A61K35/407 , A61K38/16 , A61P1/16 , A61P43/00 , A61K35/14 , A61K37/00 , C07G7/00
CPC分类号： A61K35/16 , A61K35/407 , Y10S530/83 , Y10S530/846 , Y10S530/85
摘要： A factor which causes an increase in the number of liver cells through increased cell division activity, obtained by homogenizing the remaining livers of partially hepatectomized animals, subjecting the homogenate to acidification to pH=5.5 and then heat treatment at 95.degree. C., and centrifuging said homogenate. After such treatment, the factor is contained in the supernatant. The factor is a neuraminic-acid-free protein with a molecular weight of approximately 30,000 to 50,000 D. An extract containing the factor also can be obtained from the blood plasma of hepatectomized animals, when the plasma in addition to the described steps further is treated with neuraminidase. Similarly, an extract containing the factor can be obtained from the livers or the plasma of non-hepatectomized fetal or very young animals.
摘要翻译：通过使部分肝切除动物的剩余肝脏匀浆获得的增加细胞分裂活性导致肝细胞数量增加的因素，使匀浆酸化至pH = 5.5，然后在95℃下热处理，并离心说匀浆。在这样的处理之后，该因子被包含在上清液中。该因子是分子量大约为30,000至50,000D的不含神经氨酸的蛋白质。当除了进一步治疗所述步骤之外的血浆时，也可从肝切除的动物的血浆中获得含有该因子的提取物与神经氨酸酶。类似地，可以从非肝切除的胎儿或非常年轻的动物的肝脏或血浆获得含有因子的提取物。

6. 发明授权

US4330528A Drug for stimulating the rate of proliferation of liver cells and method for producing same 失效
标题翻译：刺激肝细胞增殖率的药物及其制备方法
公开(公告)号：US4330528A
公开(公告)日：1982-05-18
申请号：US105923
申请日：1980-07-03
申请人： Gerhard Ruhenstroth-Bauer , Michel Goldberg
发明人： Gerhard Ruhenstroth-Bauer , Michel Goldberg
IPC分类号： C07K14/52 , A61K35/16 , A61K38/16 , A61P1/16 , A61P43/00 , C07K14/435 , A61K35/14 , A61K35/407 , A61K37/00
CPC分类号： A61K35/16 , Y10S530/83
摘要： A drug for stimulating the rate of proliferation of liver cells having as s active ingredient a blood plasma extract (NP) and a method of preparing such a drug wherein blood plasma is first acidified to a pH of 5.5, then heat denatured at approximately 95.degree. C. and subsequently centrifuged to provide the extract.
摘要翻译：用于刺激作为其活性成分的血浆提取物（NP）的肝细胞增殖速率的药物及其制备方法，其中首先将血浆酸化至pH5.5，然后在约95℃变性然后离心以提供提取物。

7. 发明授权

US4631957A Method for alerting patients with diseases affected by climate, such as epilepsy and myocardial infarction 失效
标题翻译：用于警惕患有气候疾病的患者，如癫痫和心肌梗死的方法
公开(公告)号：US4631957A
公开(公告)日：1986-12-30
申请号：US576520
申请日：1984-02-02
申请人： Gerhard Ruhenstroth-Bauer
发明人： Gerhard Ruhenstroth-Bauer
IPC分类号： A61B10/00 , A61N5/00 , G01W1/00 , G01W1/10 , G08B21/00 , G08B23/00
CPC分类号： A61N5/00
摘要： A method for alerting patients with diseases affected by climate, such as epilepsy and myocordial infarction, according to which sinusoidal spheric pulses having a frequency of 28 kHz and sinusoidal spheric pulses having a frequency of 10 kHz are measured by a spheric selector circuit. The pulse rate of these spheric pulses is ascertained over a predetermined period of time and the difference between the pulse rates of the spheric pulses at 28 kHz and those at 10 kHz is ascertained. If the spheric pulses having a frequency of 28 kHz predominate, then the patient is alerted by means of a signal generated by a circuit which includes the spheric selector circuit for carrying out the method.
摘要翻译：通过球形选择器电路测量具有频率为28kHz的正弦球状脉冲和频率为10kHz的正弦球状脉冲的用于警告患有受气候影响的疾病（例如癫痫和肌肉梗死）的患者的方法。在预定时间段内确定这些球状脉冲的脉冲速率，确定28kHz的球状脉冲与10kHz的脉冲之间的脉冲速率之差。如果频率为28kHz的球面脉冲占主导地位，则通过由包括用于执行该方法的球面选择器电路的电路产生的信号来警告患者。

8. 发明授权

US4377514A Method for producing factor for the stimulation of the liver cell proliferation rate 失效
标题翻译：生产刺激肝细胞增殖因子的方法
公开(公告)号：US4377514A
公开(公告)日：1983-03-22
申请号：US308355
申请日：1981-10-05
申请人： Gerhard Ruhenstroth-Bauer , Michel Goldberg , Hubertus Schneider
发明人： Gerhard Ruhenstroth-Bauer , Michel Goldberg , Hubertus Schneider
IPC分类号： A61K35/26 , A61K35/38 , A61K35/55 , A61K37/02 , C07G7/00
CPC分类号： A61K35/26 , A61K35/38 , Y10S530/837 , Y10S530/854
摘要： A process for making factor for stimulating the rate of proliferation of liver cells comprising homogenizing animal Peyer's glands, adjusting pH to about 5.5, thermally denaturing the product and recovering the desired factor in the supernatant by centrifugation.
摘要翻译：制备用于刺激肝细胞增殖速度的因素的方法，包括使动物派耶尔腺体均质化，调节pH至约5.5，热变性产物并通过离心回收上清液中的所需因子。

9. 发明授权

US4094759A Method for simultaneous quantitative analysis of several constituents in a sample 失效
标题翻译：同时定量分析样品中几种成分的方法
公开(公告)号：US4094759A
公开(公告)日：1978-06-13
申请号：US758735
申请日：1977-01-12
申请人： Gerhard Ruhenstroth-Bauer , Reiner Scherer
发明人： Gerhard Ruhenstroth-Bauer , Reiner Scherer
IPC分类号： G01N27/447 , G01N33/561 , G01N27/26
CPC分类号： G01N27/447 , G01N33/561
摘要： A method for simultaneous qualitative and quantitative analysis of antigensr antigen-effective substances in a sample provides placing the sample in a carrier medium through which it migrates toward a plurality of physically separate carrier strips. The medium on each strip contains a quantity of a specific ingredient, for example an antibody, which is expected to undergo a specific immunoreaction with one of the constituents in the sample, thereby producing a visible precipitate. The dimensions of the final precipitate then permit a direct measurement of the identified substance. A known concentration of a calibrating substance may be added to the sample to provide for calibration precipitates in the various strips. The apparatus for practicing the method includes a base plate on which the neutral, antibody-free carrier medium as well as the various strips containing media with antibodies or other immunoreactive ingredients are located. An electric field may be applied to aid in the migration of the sample in the medium.
摘要翻译：用于同时定性和定量分析样品中的抗原或抗原有效物质的方法提供将样品置于载体介质中，通过该载体介质向多个物理上分离的载体条移动。每个条带上的培养基含有一定量的特定成分，例如抗体，其预期与样品中的一种成分进行特异性免疫反应，从而产生可见的沉淀物。然后，最终沉淀物的尺寸允许直接测量所鉴定的物质。可以将已知浓度的校准物质添加到样品中以提供各种条中的校准沉淀。用于实施该方法的装置包括基板，其上定位有中性，无抗体的载体介质以及含有抗体或其它免疫反应性成分的培养基的各种条。可以施加电场以帮助样品在介质中的迁移。

10. 发明授权

US5624373A Method and apparatus for influencing such characteristics of a biological material which correlate with the occurrence of certain atmospherics 失效
标题翻译：影响与特定气氛的发生相关的生物材料的特性的方法和装置
公开(公告)号：US5624373A
公开(公告)日：1997-04-29
申请号：US298751
申请日：1994-08-31
申请人： Gerhard Ruhenstroth-Bauer
发明人： Gerhard Ruhenstroth-Bauer
IPC分类号： A61N1/16 , A61N2/04 , A61N1/44
CPC分类号： A61N1/16
摘要： A method and apparatus for influencing a biological material, both being intended to influence such characteristics are correlated with the occurrence of natural atmospherics, i.e., the proliferation rate of C6-glioma cells and the survival time of Yoshida-ascites-hepatoma rats. For this purpose, signals representing the time history of the magnetic field of certain natural atmospherics are stored, which are identified by means of distinctly pronounced frequency fractions, e.g., 10 kHz. Using these signals, corresponding magnetic field variations are reproduced as artificial atmospherics. Reproduction is effected, e.g., in a Helmholtz coil. The biological material is exposed to this magnetic field.
摘要翻译：用于影响生物材料的方法和装置，其目的是影响这些特征与自然大气的发生，即C6-神经胶质瘤细胞的增殖速率和吉田 - 腹水 - 肝细胞瘤大鼠的存活时间相关。为此，存储表示某些自然大气的磁场的时间历史的信号，其通过明显显着的频率分数（例如10kHz）来识别。使用这些信号，相应的磁场变化被再现为人造气氛。例如在亥姆霍兹线圈中进行再现。生物材料暴露于该磁场。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式