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    • 4. 发明授权
    • Preparation of water-insoluble enzyme compositions
    • 水不溶性酶组合物的制备
    • US4230803A
    • 1980-10-28
    • US911227
    • 1978-05-31
    • Guenter WeidenbachDirk Bonse
    • Guenter WeidenbachDirk Bonse
    • C12N11/14C07G7/02
    • C12N11/14
    • Enzymes are covalently bonded to porous inorganic supports by a process wherein maximum activity of enzyme in the water-insoluble enzyme composition is achieved with the lowest possible amount of enzyme. The process involves selecting an inorganic carrier having a most frequent pore-diameter that results in highest enzymatic activity possible per weight unit of the insoluble enzyme composition at the given enzyme concentration within the insoluble enzyme composition, and contacting the selected inorganic carrier with an enzyme solution containing an amount of enzyme which is sufficiently low so that the enzyme in the insoluble enzyme composition has a specific activity which is substantially the same as that of the enzyme in solution in the free state.
    • 酶通过其中以最低可能量的酶实现水不溶性酶组合物中酶的最大活性的方法共价键合到多孔无机载体上。 该方法包括选择具有最常孔径的无机载体,其导致在不溶性酶组合物内的给定酶浓度下每单位重量单位的不溶性酶组合物可能产生最高的酶活性,并使所选择的无机载体与酶溶液接触 含有足够低的酶量,使得不溶性酶组合物中的酶具有与游离状态下的溶液中的酶基本相同的比活性。