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1. 发明申请

US20100234577A1 METHODS FOR PURIFYING ANTIBODIES USING CERAMIC HYDROXYAPATITE 审中-公开
标题翻译：使用陶瓷羟基磷灰石来治疗抗体的方法
公开(公告)号：US20100234577A1
公开(公告)日：2010-09-16
申请号：US12304782
申请日：2007-06-13
申请人： Gregory J. Mazzola , Thomas M. Smith
发明人： Gregory J. Mazzola , Thomas M. Smith
IPC分类号： C07K1/22 , C07K16/00
CPC分类号： C07K1/20 , C07K1/18 , C07K16/065
摘要： This invention relates to the purification of monoclonal antibodies from mammalian cell culture fluid utilizing sequential, orthogonal chromatography and filtration techniques resulting in material of high purity and quality that is suitable for human administration. The method involves capturing an IgG product using immobilized protein A affinity chromatography, followed by at least one ion exchange technique prior to adsorbing the IgG to hydroxyapatite and selectively eluting the product in a single isocratic step to achieve purification from impurities and simultaneously reducing multiple types of impurities including but not limited to IgG aggregates, residual protein A, non-IgG proteins, host cell proteins, viral particles, and DNA
摘要翻译：本发明涉及使用顺序，正交色谱和过滤技术从哺乳动物细胞培养液中纯化单克隆抗体，产生适于人类施用的高纯度和高质量的材料。该方法包括使用固定化蛋白A亲和色谱法捕获IgG产物，接着在将IgG吸附到羟基磷灰石之前进行至少一种离子交换技术，并在单个等度梯度步骤中选择性洗脱产物，以实现纯化从杂质中同时降低多种类型杂质包括但不限于IgG聚集体，残留蛋白A，非IgG蛋白，宿主细胞蛋白，病毒颗粒和DNA

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