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1. 发明申请

US20110200989A1 SINGLE MOLECULE NUCLEIC ACID SEQUENCING USING MULTIPHOTON FLUORESCENCE EXCITATION 审中-公开
标题翻译：使用多光子荧光激发的单分子核酸序列
公开(公告)号：US20110200989A1
公开(公告)日：2011-08-18
申请号：US13009442
申请日：2011-01-19
申请人： Gordon A. Janaway , Christina E. Inman , Joseph Beechem
发明人： Gordon A. Janaway , Christina E. Inman , Joseph Beechem
IPC分类号： C12Q1/68 , C12M1/34 , G01N21/75 , G01N21/63 , G01N21/64
CPC分类号： G01N21/6452 , C12Q1/6818 , C12Q1/6869 , G01N21/6428 , G01N2021/6441 , Y10T436/143333 , C12Q2563/131 , C12Q2523/313
摘要： A system for detection of nucleic acids can include an excitation source configured to transmit excitation energy to a reaction site including a single molecule of nucleic acid reacted with a two-photon absorption moiety. The system also can include an optical system configured to focus the excitation energy transmitted from the excitation source to a focal region containing the reaction site, wherein said excitation energy within the focal region is sufficient to cause two-photon absorption by the two-photon absorption moiety. The system can further include a detector configured to detect emissions generated at the reaction site resulting from two-photon absorption of the excitation energy by the two-photon absorption moiety.
摘要翻译：用于检测核酸的系统可以包括被配置为将激发能量传递到包括与双光子吸收部分反应的单分子核酸的反应位点的激发源。该系统还可以包括配置成将从激发源传输的激发能量聚焦到包含反应位点的聚焦区域的光学系统，其中聚焦区域内的所述激发能足以通过双光子吸收引起双光子吸收部分。该系统还可以包括检测器，其被配置为检测由双光子吸收部分的激发能量的双光子吸收引起的在反应位置产生的发射。

2. 发明授权

US08572506B2 Visualization tool for qPCR genotyping data 有权
标题翻译：可视化工具，用于qPCR基因分型数据
公开(公告)号：US08572506B2
公开(公告)日：2013-10-29
申请号：US13083476
申请日：2011-04-08
申请人： Gordon A. Janaway , Evelyn Wing-Sim Chan
发明人： Gordon A. Janaway , Evelyn Wing-Sim Chan
IPC分类号： G06F3/048
CPC分类号： G06F19/18 , G06F3/0482 , G06F3/0485 , G06F19/26
摘要： Systems and methods are used to display data obtained from a qPCR instrument. Each of two or more samples is probed with a first labeling probe and a second labeling probe. A first data set is received from a qPCR instrument at a first cycle number that includes for each sample a first labeling probe intensity, and a second labeling probe intensity. A second data set is received at a second cycle number that includes for each sample a first labeling probe intensity and a second labeling probe intensity. A first plot of first labeling probe intensity as a function of second labeling probe intensity is created using the first data set. A second plot of first labeling probe intensity as a function of second labeling probe intensity is created using the second data set. The first plot and the second plot are displayed in response to user defined input to provide dynamic and real-time analysis of genotyping data.
摘要翻译：系统和方法用于显示从qPCR仪器获得的数据。用第一标记探针和第二标记探针探测两个或更多个样品中的每一个。从qPCR仪器以第一周期数接收第一数据集，其中包括针对每个样本的第一标记探针强度和第二标记探针强度。以第二周期数接收第二数据集，其中为每个样本包括第一标记探针强度和第二标记探针强度。使用第一数据集创建第一标记探针强度作为第二标记探针强度的函数的第一图。使用第二数据集创建第二标记探针强度作为第二标记探针强度的函数的第二图。显示第一个绘图和第二个绘图以响应用户定义的输入，以提供对基因分型数据的动态和实时分析。

3. 发明授权

US09322055B2 System and method for determining copies-per-unit-volume using PCR and flow control of droplets 有权
标题翻译：使用PCR和液滴的流量控制来确定每单位体积份数的系统和方法
公开(公告)号：US09322055B2
公开(公告)日：2016-04-26
申请号：US14009304
申请日：2012-03-30
申请人： Gordon A. Janaway , Mark Andersen , Kornelija Zgonc , Michael Pallas , Marcin Sikora , Casey McFarland , Ferrier N. Le , Haopeng Wang , Jian Gong , Gothami Padmabandu
发明人： Gordon A. Janaway , Mark Andersen , Kornelija Zgonc , Michael Pallas , Marcin Sikora , Casey McFarland , Ferrier N. Le , Haopeng Wang , Jian Gong , Gothami Padmabandu
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6886 , C12Q1/686 , C12Q2600/158 , C12Q2600/178 , G01N21/6486 , C12Q2537/16 , C12Q2563/143 , C12Q2563/159 , C12Q2565/626
摘要： Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.
摘要翻译：提供了用于定量样品中靶核酸的方法和系统。该方法包括形成多个离散的样本部分。多个离散样品部分中的每一个包含样品的一部分和反应混合物。该方法还包括放大多个离散样本部分以形成多个离散处理的样本部分。至少一个离散加工的样品部分含有核酸扩增反应产物。从所述多个离散处理样品部分中的至少一个检测荧光信号以确定所述至少一种靶核酸的存在。该方法还包括确定多个多个离散处理的样本部分的相应体积，以及估计样品中至少一个靶核酸的每单位体积的拷贝数。估计每单位体积的拷贝数是基于确定为在其中含有至少一种靶核酸的离散加工样品部分的数量。

4. 发明申请

US20100228496A1 Methods for the Determination of a Copy Number of a Genomic Sequence in a Biological Sample 审中-公开
标题翻译：确定生物样品中基因组序列拷贝数的方法
公开(公告)号：US20100228496A1
公开(公告)日：2010-09-09
申请号：US12720595
申请日：2010-03-09
申请人： Harrison M. Leong , Catalin Barbacioru , Gordon A. Janaway
发明人： Harrison M. Leong , Catalin Barbacioru , Gordon A. Janaway
IPC分类号： G06F17/18 , C12Q1/68 , G06F19/00
CPC分类号： G16B30/00 , C12Q1/6827 , G06N7/005 , G16B20/00 , G16B40/00
摘要： Methods for the determination of a copy number of a target genomic sequence; either a target gene or genomic sequence of interest, in a biological sample are described. Various methods utilize a model drawn from a probability density function (PDF) for the assignment of a copy number of a target genomic sequence in a biological sample. Additionally, the methods provide for the determination of a confidence value for a copy number assigned to a sample based on attributes of the sample data. Accordingly, the various methods for the determination of a copy number provide the end user with significant information for the evaluation of a copy number of a target genomic sequence; either a gene or genomic sequence of interest.
摘要翻译：用于确定靶基因组序列拷贝数的方法; 描述了生物样品中的靶基因或目的基因组序列。各种方法利用从概率密度函数（PDF）中提取的模型来分配生物样品中靶基因组序列的拷贝数。另外，这些方法提供了基于样本数据的属性确定分配给样本的副本编号的置信度值。因此，用于确定拷贝数的各种方法为终端用户提供了用于评价目标基因组序列拷贝数的重要信息; 是感兴趣的基因或基因组序列。

5. 发明申请

US20140248623A1 System and Method for Determining Copies-per-Unit-Volume Using PCR and Flow Control of Droplets 有权
标题翻译：使用PCR和流量控制液滴确定每单位体积份数的系统和方法
公开(公告)号：US20140248623A1
公开(公告)日：2014-09-04
申请号：US14009304
申请日：2012-03-30
申请人： Gordon A. Janaway , Mark Andersen , Kornelija Zgonc , Michael Pallas , Marcin Sikora , Casey McFarland , Ferrier N. Le , Haopeng Wang , Jian Gong , Gothami Padmabandu
发明人： Gordon A. Janaway , Mark Andersen , Kornelija Zgonc , Michael Pallas , Marcin Sikora , Casey McFarland , Ferrier N. Le , Haopeng Wang , Jian Gong , Gothami Padmabandu
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/686 , C12Q2600/158 , C12Q2600/178 , G01N21/6486 , C12Q2537/16 , C12Q2563/143 , C12Q2563/159 , C12Q2565/626
摘要： Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.
摘要翻译：提供了用于定量样品中靶核酸的方法和系统。该方法包括形成多个离散的样本部分。多个离散样品部分中的每一个包含样品的一部分和反应混合物。该方法还包括放大多个离散样本部分以形成多个离散处理的样本部分。至少一个离散加工的样品部分含有核酸扩增反应产物。从所述多个离散处理样品部分中的至少一个检测荧光信号以确定所述至少一种靶核酸的存在。该方法还包括确定多个多个离散处理的样本部分的相应体积，以及估计样品中至少一个靶核酸的每单位体积的拷贝数。估计每单位体积的拷贝数是基于确定为在其中含有至少一种靶核酸的离散加工样品部分的数量。

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