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1. 发明授权

US06787357B2 Plasma-derived fibrin-based matrices and tissue 失效
标题翻译：血浆衍生的基于纤维蛋白的基质和组织
公开(公告)号：US06787357B2
公开(公告)日：2004-09-07
申请号：US09970651
申请日：2001-10-05
申请人： Gary L. Bowlin , Gary Wnek , David G. Simpson , Philippe Lam , Marcus E. Carr , Helen Fillmore
发明人： Gary L. Bowlin , Gary Wnek , David G. Simpson , Philippe Lam , Marcus E. Carr , Helen Fillmore
IPC分类号： C12N502
CPC分类号： A61L27/3834 , A61K35/12 , A61L15/32 , A61L27/225 , A61L27/3616 , A61L2400/04 , B29C41/006 , C07K14/75 , C12N5/0062 , C12N5/0068 , C12N5/0622 , C12N2503/00 , C12N2510/00 , C12N2533/56
摘要： The invention is directed to use of fibrin as an extracellular matrix and, together with cells, its use in forming engineered tissue. The engineered tissue can include the synthetic manufacture of specific organs or “organ-like” tissue. A preferred embodiment is a plasma-derived fibrin matrix containing cells.
摘要翻译：本发明涉及使用纤维蛋白作为细胞外基质，并与细胞一起用于形成工程化组织。工程组织可以包括特定器官或“器官样”组织的合成制造。优选的实施方案是含有细胞的血浆衍生的纤维蛋白基质。

2. 发明授权

US06316181B1 Establishment of cell lines with persistent expression of a green fluorescent protein (GFP) using a pIRES/EGFP DNA vector construct 失效
标题翻译：使用pIRES / EGFP DNA载体构建体建立具有绿色荧光蛋白（GFP）的持续表达的细胞系
公开(公告)号：US06316181B1
公开(公告)日：2001-11-13
申请号：US09296808
申请日：1999-04-23
申请人： Helen Fillmore , William C. Broaddus , John S. Shurm, Jr. , George T. Gillies
发明人： Helen Fillmore , William C. Broaddus , John S. Shurm, Jr. , George T. Gillies
IPC分类号： C12Q102
CPC分类号： G01N33/5011 , C12N5/0693 , C12N2503/00 , C12N2510/00 , C12Q1/6897
摘要： The present invention provides a vector for stably transforming cells. The vector comprises the green fluorescent protein (GFP) from Aequorea victoria as a marker. The invention also provides stably transfected cell lines which may be used to assess real-time biological processes, including tumor cell migration.
摘要翻译：本发明提供了用于稳定转化细胞的载体。载体包含来自维他命A的绿色荧光蛋白（GFP）作为标记。本发明还提供可用于评估实时生物过程（包括肿瘤细胞迁移）的稳定转染的细胞系。

3. 发明申请

US20050158286A1 Differentiation of specialized dermal and epidermal cells into neuronal cells 失效
标题翻译：专门的皮肤和表皮细胞分化成神经细胞
公开(公告)号：US20050158286A1
公开(公告)日：2005-07-21
申请号：US11022805
申请日：2004-12-28
申请人： Helen Fillmore , Shelley Hoover , William Broaddus , George Gillies
发明人： Helen Fillmore , Shelley Hoover , William Broaddus , George Gillies
IPC分类号： C12N5/071 , A61K45/00 , C12N5/08
CPC分类号： C12N5/0629 , C12N5/0618 , C12N5/0626 , C12N2506/091 , C12N2510/00
摘要： Cells are generated from skin biopsies for use in cell implantation by identifying a source of skin cells that have a surface concentration of at least one cell selected from the group consisting of keritinocytes and/or melanocytes; taking a sample of tissue from the surface area; mechanically disaggregating the tissue samples; collecting the disaggregated cells; washing the disaggregated cells; filtering the washed disaggregated cells; providing a cell suspension with filtered and washed keritinocytes and/or melanocytes; and suspending the cell suspension in a medium. In greater particularity, the method may comprise a) identifying a source of skin cells that have a surface concentration of at least 400 cells per square millimeter of surface area of at least one cell selected from the group consisting of keritinocytes and/or melanocytes; b) taking a sample of those skin cells and culturing them to enrich for cells selected from the group consisting of keritinocytes and melanocytes to form cultured cells for transplantation purposes c) concentrating the cells selected from the group consisting of keritinocytes and/or melanocytes from the cultured mass of cells; and d) differentiating the concentrated cells selected from the group consisting of keritinocytes an/or melanocytes.
摘要翻译：通过鉴定具有至少一种选自角质蛋白和/或黑素细胞的细胞的表面浓度的皮肤细胞来源，从皮肤活检中产生细胞，用于细胞植入; 从表面区域取样组织; 机械分解组织样品; 收集分解细胞; 洗涤分解细胞; 过滤洗涤的分解细胞; 提供具有过滤和洗涤的角质细胞和/或黑素细胞的细胞悬浮液; 并将细胞悬浮液悬浮于培养基中。更具体地，该方法可以包括：a）鉴定表皮浓度为至少一个选自下组的角质形成细胞和/或黑素细胞的细胞每平方毫米的平均毫米表面积的皮肤细胞来源; b）取这些皮肤细胞的样品并培养它们以富集选自由角质细胞和黑素细胞组成的组的细胞，以形成用于移植目的的培养细胞; c）将选自下组的细胞和/或黑素细胞培养细胞数量; 和d）区分选自由黑素细胞或黑素细胞组成的组的浓缩细胞。

4. 发明授权

US06410597B1 Hydroxyalkyl amide analogs of ceramide 失效
标题翻译：神经酰胺的羟烷基酰胺类似物
公开(公告)号：US06410597B1
公开(公告)日：2002-06-25
申请号：US09512597
申请日：2000-02-25
申请人： Erhard Bieberich , Raphael M. Ottenbrite , Robert K. Yu , Helen Fillmore , William C. Broaddus
发明人： Erhard Bieberich , Raphael M. Ottenbrite , Robert K. Yu , Helen Fillmore , William C. Broaddus
IPC分类号： A61K3116
CPC分类号： A61K31/045 , A61K31/16
摘要： The present invention provides methods for treating disease conditions associated with abnormal cellular proliferation, inflammation and viral infection or proliferation by the administration of ceramic analogs. The ceramic analogs appear to be specific for modulation of the enzyme PKC&zgr;.
摘要翻译：本发明提供了通过给予陶瓷类似物来治疗与异常细胞增殖，炎症和病毒感染或增殖相关的疾病状况的方法。陶瓷类似物似乎对酶PKC＆zgr的调节是特异性的。

5. 发明申请

US20080233644A1 Chimeric Transcription Factor Decoy Oligonucleotides 审中-公开
标题翻译：嵌合转录因子诱饵寡核苷酸
公开(公告)号：US20080233644A1
公开(公告)日：2008-09-25
申请号：US10570160
申请日：2004-09-10
申请人： Helen Fillmore , Jessicca I. Martin Rege
发明人： Helen Fillmore , Jessicca I. Martin Rege
IPC分类号： C12N5/02 , C07H21/00
CPC分类号： C12N9/6491 , C12N15/1135 , C12N15/115 , C12N2310/13
摘要： A method for the treatment of the brain cancer glioblastoma multiforme (GBM) is provided. The method involves decreasing the expression of Matrix Metalloproteinase-1 (MMP-1) expression by providing transcription factor decoy nucleotides that mimic single nucleotide polymorphisms responsible for MMP-1 overexpression.
摘要翻译：提供了一种治疗脑癌胶质母细胞瘤多形性（GBM）的方法。该方法通过提供模拟负责MMP-1过表达的单核苷酸多态性的转录因子诱饵核苷酸来降低基质金属蛋白酶-1（MMP-1）表达的表达。

6. 发明授权

US07361506B2 Differentiation of specialized dermal and epidermal cells into neuronal cells 失效
标题翻译：专门的皮肤和表皮细胞分化成神经细胞
公开(公告)号：US07361506B2
公开(公告)日：2008-04-22
申请号：US11022805
申请日：2004-12-28
申请人： Helen Fillmore , Shelley Hoover , William C. Broaddus , George Gillies
发明人： Helen Fillmore , Shelley Hoover , William C. Broaddus , George Gillies
IPC分类号： C12N5/00 , C12N5/02 , C12N5/08
CPC分类号： C12N5/0629 , C12N5/0618 , C12N5/0626 , C12N2506/091 , C12N2510/00
摘要： Cells are generated from skin biopsies for use in cell implantation by identifying a source of skin cells that have a surface concentration of at least one cell selected from the group consisting of keritinocytes and/or melanocytes; taking a sample of tissue from the surface area; mechanically disaggregating the tissue samples; collecting the disaggregated cells; washing the disaggregated cells; filtering the washed disaggregated cells; providing a cell suspension with filtered and washed keritinocytes and/or melanocytes; and suspending the cell suspension in a medium. In greater particularity, the method may comprise a) identifying a source of skin cells that have a surface concentration of at least 400 cells per square millimeter of surface area of at least one cell selected from the group consisting of keritinocytes and/or melanocytes; b) taking a sample of those skin cells and culturing them to enrich for cells selected from the group consisting of keritinocytes and melanocytes to form cultured cells for transplantation purposes c) concentrating the cells selected from the group consisting of keritinocytes and/or melanocytes from the cultured mass of cells; and d) differentiating the concentrated cells selected from the group consisting of keritinocytes an/or melanocytes.
摘要翻译：通过鉴定具有至少一种选自角质蛋白和/或黑素细胞的细胞的表面浓度的皮肤细胞来源，从皮肤活检中产生细胞，用于细胞植入; 从表面区域取样组织; 机械分解组织样品; 收集分解细胞; 洗涤分解细胞; 过滤洗涤的分解细胞; 提供具有过滤和洗涤的角质细胞和/或黑素细胞的细胞悬浮液; 并将细胞悬浮液悬浮于培养基中。更具体地，该方法可以包括：a）鉴定表皮浓度为至少一个选自下组的角质形成细胞和/或黑素细胞的细胞每平方毫米的平均毫米表面积的皮肤细胞来源; b）取这些皮肤细胞的样品并培养它们以富集选自由角质细胞和黑素细胞组成的组的细胞，以形成用于移植目的的培养细胞; c）将选自下组的细胞和/或黑素细胞培养细胞数量; 和d）区分选自由黑素细胞或黑素细胞组成的组的浓缩细胞。

7. 发明授权

US06277571B1 Sequential consensus region-directed amplification of known and novel members of gene families 失效
标题翻译：已知和新型基因家族成员的序列一致区域定向扩增
公开(公告)号：US06277571B1
公开(公告)日：2001-08-21
申请号：US09163485
申请日：1998-09-30
申请人： Helen Fillmore , William Broaddus , George Gillies
发明人： Helen Fillmore , William Broaddus , George Gillies
IPC分类号： C12Q168
CPC分类号： C12Q1/6883 , C12Q1/6809 , C12Q2549/119 , C12Q2525/15
摘要： A method of sequential consensus region-directed amplification comprising, (a) amplifying a first segment of at least one target DNA in a DNA mixture, using a first and second oligonucleotide primer, each of which hybridizes to the target DNA, and a nucleic acid polymerase, under conditions in which DNA amplification is achieved, resulting in a first segment of double-stranded DNA; (b) amplifying a second segment of the first segment of double-stranded DNA, using a third and fourth oligonucleotide primer, each of which hybridizes to the first DNA segment, and a nucleic acid polymerase, under conditions in which DNA amplification is achieved, resulting in a second segment of double-stranded DNA.
摘要翻译：一种连续共有区域定向扩增的方法，其包括：（a）使用与目标DNA杂交的第一和第二寡核苷酸引物和核酸（a）扩增DNA混合物中的至少一种靶DNA的第一片段在实现DNA扩增的条件下，产生第一段双链DNA; （b）在实现DNA扩增的条件下，使用与第一DNA片段杂交的第三和第四寡核苷酸引物和核酸聚合酶扩增第二片段的双链DNA，导致第二段双链DNA。

8. 发明授权

US08406837B2 Means and methods for cytometric therapies 有权
标题翻译：细胞疗法的方法和方法
公开(公告)号：US08406837B2
公开(公告)日：2013-03-26
申请号：US12513258
申请日：2007-11-01
申请人： George T. Gillies , Helen Fillmore , William C. Broaddus , Boyd M. Evans, III , Stephen W. Allison
发明人： George T. Gillies , Helen Fillmore , William C. Broaddus , Boyd M. Evans, III , Stephen W. Allison
IPC分类号： A61B5/00
CPC分类号： A61B5/0084 , A61B5/0071 , A61B5/0075
摘要： A functionalized tip is incorporated into catheters for the cytometric delivery of cells into the brain and other body parts. For use in the brain, the tip forms part of a neurosurgical probe having a proximal end and a distal end. In addition to the functionalized tip, the probe has at least one cell slurry delivery lumen and a plurality of optical fibers configured along the probe, terminating in the tip to provide the photo-optical capability needed to monitor the viability and physiological behavior of the grafted cells as well as certain characteristics of the cellular environment. Details are also presented of the use of a neurocatheter having a cytometric tip of the type disclosed in the invention, as employed within the context of a feedback and control system for regulating the number of cells delivered to the brain of a patient.
摘要翻译：一个功能化的尖端被并入到导管中，用于将细胞细胞传递到脑和其他身体部位。为了在脑中使用，尖端形成具有近端和远端的神经外科探针的一部分。除了功能化的尖端之外，探针具有至少一个细胞浆液递送腔和沿着探针配置的多个光纤，终止于尖端，以提供监测嫁接的生存力和生理行为所需的光学光学能力细胞以及细胞环境的某些特征。还提供了在用于调节递送到患者脑部的细胞数量的反馈和控制系统的上下文中使用的具有本发明公开的类型的细胞计数尖端的神经导管的用途的细节。

9. 发明申请

US20100210927A1 MEANS AND METHODS FOR CYTOMETRIC THERAPIES 有权
标题翻译：细胞治疗方法和方法
公开(公告)号：US20100210927A1
公开(公告)日：2010-08-19
申请号：US12513258
申请日：2007-11-01
申请人： George T. Gillies , Helen Fillmore , William C. Broaddus , Boyd M. Evans , Stephen W. Allison
发明人： George T. Gillies , Helen Fillmore , William C. Broaddus , Boyd M. Evans , Stephen W. Allison
IPC分类号： A61B6/12 , A61B6/00 , A61B5/055
CPC分类号： A61B5/0084 , A61B5/0071 , A61B5/0075
摘要： A functionalized tip is incorporated into catheters for the cytometric delivery of cells into the brain and other body parts. For use in the brain, the tip forms part of a neurosurgical probe having a proximal end and a distal end. In addition to the functionalized tip, the probe has at least one cell slurry delivery lumen and a plurality of optical fibers configured along the probe, terminating in the tip to provide the photo-optical capability needed to monitor the viability and physiological behavior of the grafted cells as well as certain characteristics of the cellular environment. Details are also presented of the use of a neurocatheter having a cytometric tip of the type disclosed in the invention, as employed within the context of a feedback and control system for regulating the number of cells delivered to the brain of a patient.
摘要翻译：一个功能化的尖端被并入到导管中，用于将细胞细胞传递到脑和其他身体部位。为了在脑中使用，尖端形成具有近端和远端的神经外科探针的一部分。除了功能化的尖端之外，探针具有至少一个细胞浆液递送腔和沿着探针配置的多个光纤，终止于尖端，以提供监测嫁接的生存力和生理行为所需的光学光学能力细胞以及细胞环境的某些特征。还提供了在用于调节递送到患者脑部的细胞数量的反馈和控制系统的上下文中使用的具有本发明公开的类型的细胞计数尖端的神经导管的用途的细节。

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