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1. 发明申请

US20050064474A1 Methods and compositions for targeted cleavage and recombination 有权
标题翻译：用于靶向切割和重组的方法和组合物
公开(公告)号：US20050064474A1
公开(公告)日：2005-03-24
申请号：US10912932
申请日：2004-08-06
申请人： Fyodor Urnov , Michael Holmes , Jeffrey Miller , Carl Pabo
发明人： Fyodor Urnov , Michael Holmes , Jeffrey Miller , Carl Pabo
IPC分类号： C07K14/435 , C07K14/47 , C07K14/715 , C12N9/14 , C12N9/22 , C12N15/62 , C12P19/34 , C12Q1/68
CPC分类号： A61K35/12 , C07K14/43595 , C07K14/4717 , C07K14/715 , C07K2319/00 , C07K2319/09 , C07K2319/81 , C12N9/14 , C12N9/22 , C12N15/62 , C12N15/85 , C12N2800/80
摘要： Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.
摘要翻译：本文公开了用于基因组序列的靶向切割，基因组序列的靶向改变以及基因组区域和与该基因组区域同源的外源多核苷酸之间的靶向重组的方法和组合物。组合物包括包含切割结构域（或切割半结构域）和工程化锌指结构域的融合蛋白和编码它们的多核苷酸。用于靶向切割的方法包括将这种融合蛋白或其编码的多核苷酸引入细胞。用于靶向重组的方法另外包括将与基因组区域同源的外源多核苷酸引入包含所公开的融合蛋白的细胞中。

2. 发明申请

US20080182332A1 Optimized non-canonical zinc finger proteins 有权
标题翻译：优化非规范锌指蛋白
公开(公告)号：US20080182332A1
公开(公告)日：2008-07-31
申请号：US12001939
申请日：2007-12-13
申请人： Qihua C. Cai , Vipula K. Shukla , Joseph F. Petolino , Lisa W. Baker , Robbi J. Garrison , Ryan C. Blue , Jon C. Mitchell , Nicole L. Arnold , Sarah E. Worden , Jeffrey Miller , Fyodor Urnov
发明人： Qihua C. Cai , Vipula K. Shukla , Joseph F. Petolino , Lisa W. Baker , Robbi J. Garrison , Ryan C. Blue , Jon C. Mitchell , Nicole L. Arnold , Sarah E. Worden , Jeffrey Miller , Fyodor Urnov
IPC分类号： C12N15/82 , C07K16/00 , C12N5/00 , C07H21/04
CPC分类号： C07K14/4702 , C07K2319/81 , C12N15/8213 , C12N15/8243
摘要： Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.
摘要翻译：本文公开了包含CCHC锌配位残基的锌指。还描述了锌指蛋白和包含这些CCHC锌指的融合蛋白以及编码这些蛋白的多核苷酸。还描述了使用这些蛋白质进行基因编辑和基因调控的方法。

3. 发明申请

US20090111188A1 Optimized non-canonical zinc finger proteins 有权
标题翻译：优化非规范锌指蛋白
公开(公告)号：US20090111188A1
公开(公告)日：2009-04-30
申请号：US12286668
申请日：2008-10-01
申请人： Qihua C. Cai , Vipula K. Shukla , Joseph F. Petolino , Lisa W. Baker , Robbi J. Garrison , Ryan C. Blue , Jon C. Mitchell , Nicole L. Arnold , Sarah E. Worden , Jeffrey Miller , Fyodor Urnov
发明人： Qihua C. Cai , Vipula K. Shukla , Joseph F. Petolino , Lisa W. Baker , Robbi J. Garrison , Ryan C. Blue , Jon C. Mitchell , Nicole L. Arnold , Sarah E. Worden , Jeffrey Miller , Fyodor Urnov
IPC分类号： C12N15/10
CPC分类号： C07K14/4702 , C07K2319/81 , C12N15/8213 , C12N15/8243
摘要： Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.
摘要翻译：本文公开了包含CCHC锌配位残基的锌指。还描述了锌指蛋白和包含这些CCHC锌指的融合蛋白以及编码这些蛋白的多核苷酸。还描述了使用这些蛋白质进行基因编辑和基因调控的方法。

4. 发明授权

US09187758B2 Optimized non-canonical zinc finger proteins 有权
公开(公告)号：US09187758B2
公开(公告)日：2015-11-17
申请号：US12001939
申请日：2007-12-13
申请人： Qihua C. Cai , Vipula K. Shukla , Joseph F. Petolino , Lisa W. Baker , Robbi J. Garrison , Ryan C. Blue , Jon C. Mitchell , Nicole L. Arnold , Sarah E. Worden , Jeffrey Miller , Fyodor Urnov
发明人： Qihua C. Cai , Vipula K. Shukla , Joseph F. Petolino , Lisa W. Baker , Robbi J. Garrison , Ryan C. Blue , Jon C. Mitchell , Nicole L. Arnold , Sarah E. Worden , Jeffrey Miller , Fyodor Urnov
IPC分类号： C12N15/82 , C07K19/00 , C12N15/62
CPC分类号： C07K14/4702 , C07K2319/81 , C12N15/8213 , C12N15/8243
摘要： Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.

5. 发明授权

US08921112B2 Optimized non-canonical zinc finger proteins 有权
标题翻译：优化非规范锌指蛋白
公开(公告)号：US08921112B2
公开(公告)日：2014-12-30
申请号：US12286668
申请日：2008-10-01
申请人： Qihua C. Cai , Vipula K. Shukla , Joseph F. Petolino , Lisa W. Baker , Robbi J. Garrison , Ryan C. Blue , Jon C. Mitchell , Nicole L. Arnold , Sarah E. Worden , Jeffrey Miller , Fyodor Urnov
发明人： Qihua C. Cai , Vipula K. Shukla , Joseph F. Petolino , Lisa W. Baker , Robbi J. Garrison , Ryan C. Blue , Jon C. Mitchell , Nicole L. Arnold , Sarah E. Worden , Jeffrey Miller , Fyodor Urnov
IPC分类号： C12N15/82 , C12N5/04 , A01H5/10 , A01H1/08 , C12N5/10
CPC分类号： C07K14/4702 , C07K2319/81 , C12N15/8213 , C12N15/8243
摘要： Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.
摘要翻译：本文公开了包含CCHC锌配位残基的锌指。还描述了锌指蛋白和包含这些CCHC锌指的融合蛋白以及编码这些蛋白的多核苷酸。还描述了使用这些蛋白质进行基因编辑和基因调控的方法。

6. 发明授权

US07939327B2 Simultaneous modulation of multiple genes 有权
标题翻译：同时调控多种基因
公开(公告)号：US07939327B2
公开(公告)日：2011-05-10
申请号：US12072871
申请日：2008-02-28
申请人： Jeffrey Miller , Guofu Li , Carl Pabo , Trevor Collingwood
发明人： Jeffrey Miller , Guofu Li , Carl Pabo , Trevor Collingwood
IPC分类号： C12N15/82 , C12N5/14
CPC分类号： C07K14/4702
摘要： Disclosed herein are compositions and methods that regulate expression of two or more endogenous genes.
摘要翻译：本文公开了调节两个或更多个内源基因表达的组合物和方法。

7. 发明授权

US07361635B2 Simultaneous modulation of multiple genes 有权
公开(公告)号：US07361635B2
公开(公告)日：2008-04-22
申请号：US10651761
申请日：2003-08-29
申请人： Jeffrey Miller , Guofu Li , Carl Pabo , Trevor Collingwood
发明人： Jeffrey Miller , Guofu Li , Carl Pabo , Trevor Collingwood
IPC分类号： A61K38/00 , C12P21/06 , C07K14/00 , C07H21/04 , G01N33/53
CPC分类号： C07K14/4702
摘要： Disclosed herein are compositions and methods that regulate expression of two or more endogenous genes.

8. 发明申请

US20080233641A1 Simultaneous modulation of multiple genes 有权
标题翻译：同时调控多种基因
公开(公告)号：US20080233641A1
公开(公告)日：2008-09-25
申请号：US12072871
申请日：2008-02-28
申请人： Jeffrey Miller , Guofu Li , Carl Pabo , Trevor Collingwood
发明人： Jeffrey Miller , Guofu Li , Carl Pabo , Trevor Collingwood
IPC分类号： C12N5/06 , C12N5/04
CPC分类号： C07K14/4702
摘要： Disclosed herein are compositions and methods that regulate expression of two or more endogenous genes.
摘要翻译：本文公开了调节两个或更多个内源基因表达的组合物和方法。

9. 发明申请

US20050064477A1 Methods and compositions for interaction trap assays 有权
标题翻译：相互作用阱测定法的方法和组成
公开(公告)号：US20050064477A1
公开(公告)日：2005-03-24
申请号：US10915233
申请日：2004-08-10
申请人： J. Joung , Jeffrey Miller , Carl Pabo
发明人： J. Joung , Jeffrey Miller , Carl Pabo
IPC分类号： C12N15/10 , C12Q1/68 , G01N33/50
CPC分类号： C12N15/1055 , C12Q1/6897 , G01N33/50 , G01N33/5008 , G01N33/502 , C12Q2565/201
摘要： The present invention provides methods and compositions for interaction trap assays for detecting protein-protein, protein-DNA, or protein-RNA interactions. The methods and compositions of the invention may also be used to identify agents which may agonize or antagonize a protein-protein, protein-DNA, or protein-RNA interactions. In certain embodiments, the interaction trap system of the invention is useful for screening libraries with greater than 107 members. In other embodiments, the interaction trap system of the invention is used in conjunction with flow cytometry. The invention further provides a means for simultaneously screening a target protein or nucleic acid sequence for the ability to interact with two or more test proteins or nucleic acids.
摘要翻译：本发明提供了用于检测蛋白质 - 蛋白质，蛋白质-DNA或蛋白质 - RNA相互作用的相互作用阱测定法的方法和组合物。本发明的方法和组合物还可用于鉴定可能激动或拮抗蛋白质 - 蛋白质，蛋白质-DNA或蛋白质 - RNA相互作用的试剂。在某些实施方案中，本发明的相互作用捕获系统可用于筛选具有大于10 7个成员的文库。在其它实施方案中，本发明的相互作用阱系统结合流式细胞术使用。本发明还提供了用于同时筛选靶蛋白或核酸序列以获得与两种或多种测试蛋白或核酸相互作用的能力的方法。

10. 外观设计

USD810518S1 Combined fish cleaner grate and tray 有权
公开(公告)号：USD810518S1
公开(公告)日：2018-02-20
申请号：US29620353
申请日：2016-10-26
申请人： Jeffrey Miller
设计人： Jeffrey Miller

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