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1. 发明授权

US07271311B2 Mammalian model for diabetes 失效
标题翻译：哺乳动物模型糖尿病
公开(公告)号：US07271311B2
公开(公告)日：2007-09-18
申请号：US10252111
申请日：2002-09-23
申请人： Fumitoshi Ishino , Naoki Miyoshi , Tomoko Ishino , Minesuke Yokoyama , Shigeharu Wakana
发明人： Fumitoshi Ishino , Naoki Miyoshi , Tomoko Ishino , Minesuke Yokoyama , Shigeharu Wakana
IPC分类号： G01N33/00 , A01K67/27 , A01K67/33
CPC分类号： C12N15/8509 , A01K67/0273 , A01K67/0275 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/105 , A01K2267/03 , A01K2267/0306 , C07K14/4705
摘要： An object of the present invention is to provide a model mammal for diabetes onset, being useful for elucidating the onset mechanism of diabetes caused by a blockage of signal transduction from insulin, and for the development of a remedy for said diabetes, and to provide a screening method of a remedy for said diabetes. A diabetic-prone transgenic mouse is prepared in a process comprising the steps of: a transgene that contains a Meg1/Grb10 gene, an imprinted gene exhibiting maternal expression, or a human GRB10 gene in the downstream of a chicken β-actin promoter and in the upstream of a rabbit β-globin poly A is constructed, and subsequently the transgene is microinjected into a male proneucleus of a mouse fertilized egg; thus obtained egg cell is cultured and then transplanted into an oviduct of a pseudopregnant female mouse; after rearing up the recipient animal, baby mice that have the above-mentioned cDNA are selected from the mice born from the recipient animal.
摘要翻译：本发明的目的是提供一种用于糖尿病发作的模型哺乳动物，可用于阐明由胰岛素信号传导阻断引起的糖尿病发病机制，以及用于开发所述糖尿病的治疗方法，并提供一种所述糖尿病治疗方法的筛选方法。制备具有糖尿病倾向的转基因小鼠，该方法包括以下步骤：在鸡β-肌动蛋白启动子的下游含有Meg1 / Grb10基因，表达母体表达的印记基因或人GRB10基因的转基因构建了兔β-珠蛋白聚A的上游，随后将转基因显微注射入小鼠受精卵的雄性易位; 由此获得的卵细胞被培养，然后移植到假孕女性小鼠的输卵管中; 在饲养受体动物后，具有上述cDNA的婴儿小鼠选自从受体动物出生的小鼠。

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