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1. 发明公开

EP0954564A1 METHODS FOR GENERATING CYTOTOXIC T CELLS IN VITRO 失效
标题翻译： PROCEDURE FOR GENERATING细胞毒性T细胞在体外
公开(公告)号：EP0954564A1
公开(公告)日：1999-11-10
申请号：EP97910831.0
申请日：1997-10-06
申请人： Fordham University
发明人： SRIVASTAVA, Pramod, K. , BINDER, Robert , BLACHERE, Nathalie, E.
IPC分类号： C12N5 , A61K35 , A61K38 , A61K39 , A61P31 , A61P35 , A61P37
CPC分类号： A61K39/0011 , A61K2039/5158
摘要： The present invention provides methods for generating antigen-reative cytotoxic T cells in vitro comprising culturing immune cells and antigenic cells that have at least one MHC allele in common (and preferably, are syngeneic), in which the antigenic cells have been treated according to the methods of the invention. The antigenic cells are treated by subjecting them to osmotic shock followed by irradiation. As a result, a subset of T cells are activated and mature into antigen-reactive cytotoxic T cells. The effectiveness of the procedure may be enhanced by repeated restimulations and/or the addition of heat shock protein-peptide complexes. Methods and compositions are also disclosed for the treatment and prevention in a subject of cancer or infectious disease comprising administering to the subject matched cytotoxic T cells that are generated in vitro by the present methods.

2. 发明申请

WO1998015616A1 METHODS FOR GENERATING CYTOTOXIC T CELLS IN VITRO 审中-公开
标题翻译：在体内产生细胞毒素T细胞的方法
公开(公告)号：WO1998015616A1
公开(公告)日：1998-04-16
申请号：PCT/US1997018110
申请日：1997-10-06
申请人： FORDHAM UNIVERSITY
发明人： FORDHAM UNIVERSITY , SRIVASTAVA, Pramod, K. , BINDER, Robert , BLACHERE, Nathalie, E.
IPC分类号： C12N05/06
CPC分类号： A61K39/0011 , A61K2039/5158
摘要： The present invention provides methods for generating antigen-reative cytotoxic T cells in vitro comprising culturing immune cells and antigenic cells that have at least one MHC allele in common (and preferably, are syngeneic), in which the antigenic cells have been treated according to the methods of the invention. The antigenic cells are treated by subjecting them to osmotic shock followed by irradiation. As a result, a subset of T cells are activated and mature into antigen-reactive cytotoxic T cells. The effectiveness of the procedure may be enhanced by repeated restimulations and/or the addition of heat shock protein-peptide complexes. Methods and compositions are also disclosed for the treatment and prevention in a subject of cancer or infectious disease comprising administering to the subject matched cytotoxic T cells that are generated in vitro by the present methods.
摘要翻译：本发明提供了在体外产生抗原性细胞毒性T细胞的方法，包括培养具有至少一种共同（优选是同基因）的MHC等位基因的免疫细胞和抗原性细胞，其中抗原细胞已经根据本发明的方法。通过对抗原细胞进行渗透休克，然后照射进行处理。结果，一部分T细胞被激活并成熟为抗原反应性细胞毒性T细胞。可以通过重复的再刺激和/或加入热休克蛋白 - 肽复合物来增强该程序的有效性。还公开了用于癌症或感染性疾病的受试者的治疗和预防的方法和组合物，包括向受试者施用通过本发明方法在体外产生的匹配的细胞毒性T细胞。

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