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1. 发明专利

JP2012072163A Use of amino-oxy functional group in preparation of vaccine 审中-公开
标题翻译：氨基氧官能团用于制备疫苗的应用
公开(公告)号：JP2012072163A
公开(公告)日：2012-04-12
申请号：JP2011252372
申请日：2011-11-18
申请人： Fina Biosolutions Llc , フィナバイオソリューションズリミテッドライアビリティカンパニー
发明人： LEES ANDREW
IPC分类号： A61K39/00 , A61K47/48
CPC分类号： A61K47/646 , A61K47/61
摘要： PROBLEM TO BE SOLVED: To provide a method for covalently linking proteins and polysaccharides to form conjugate vaccines comprising a reaction between carbonyl-containing groups and amino-oxy functional groups.SOLUTION: The method for preparing a conjugate comprising a process of combining an amino-oxy homofunctional or heterofunctional reagent with an entity chosen from polysaccharides, oligosaccharides, carbohydrates, and carbohydrate-containing molecules containing at least one carbonyl group, to form a polysaccharide, oligosaccharide, carbohydrate, or carbohydrate-containing molecule functionalized via at least one oxime linkage. The functionalized compound is reacted either directly or indirectly with a protein moiety to form a protein-carbohydrate conjugate that may be used as a vaccine.
摘要翻译：待解决的问题：提供共价连接蛋白质和多糖以形成包含含羰基团和氨基 - 氧基官能团之间的反应的缀合物疫苗的方法。解决方案：制备缀合物的方法，其包括将氨基 - 氧基官能或异官能试剂与选自含有至少一个羰基的多糖，寡糖，碳水化合物和含碳水化合物的分子的实体组合的方法，以形成多糖，寡糖，碳水化合物或含碳水化合物的分子通过至少一个肟键官能化。官能化化合物直接或间接与蛋白质部分反应以形成可用作疫苗的蛋白质 - 碳水化合物缀合物。版权所有（C）2012，JPO＆INPIT

2. 发明申请

WO2011017101A3 METHOD FOR PRODUCING PROTEIN-CARBOHYDRATE VACCINES REDUCED IN FREE CARBOHYDRATE 审中-公开
标题翻译：生产在免费碳水化合物中减少的蛋白质 - 碳酸氢盐霉菌的方法
公开(公告)号：WO2011017101A3
公开(公告)日：2011-06-30
申请号：PCT/US2010043387
申请日：2010-07-27
申请人： FINA BIOSOLUTIONS LLC , LEES ANDREW
发明人： LEES ANDREW
IPC分类号： A61K47/48 , A61K47/36 , A61K47/42 , C07K17/10
CPC分类号： A61K47/4833 , A61K47/36 , A61K47/42 , A61K47/6415 , A61K47/646
摘要： This invention is directed to processes for reducing the level of free carbohydrate from a solution of protein-linked carbohydrate (conjugate) and non-linked carbohydrate. In this process, the conjugate is adsorbed to a hydrophobic membrane while the carbohydrate is not. The conjugate is then desorbed from the membrane, yielding a solution that is substantially reduced in free carbohydrate.
摘要翻译：本发明涉及从蛋白质连接的碳水化合物（缀合物）和非连接的碳水化合物的溶液中降低游离碳水化合物的水平的方法。在这个过程中，当碳水化合物不结合物时，缀合物被吸附到疏水膜上。然后将缀合物从膜解吸，产生游离碳水化合物显着降低的溶液。

3. 发明申请

WO2013188539A2 DIFFERENTIAL FUNCTIONALIZATION OF POLYMERS WITH AMINO-OXY REAGENTS FOR DIAGNOSTIC ASSAYS 审中-公开
标题翻译：聚氨酯与氨基氧化试剂在诊断测定中的差异功能化
公开(公告)号：WO2013188539A2
公开(公告)日：2013-12-19
申请号：PCT/US2013045413
申请日：2013-06-12
申请人： FINA BIOSOLUTIONS LLC
发明人： LEES ANDREW
IPC分类号： C08B37/02
CPC分类号： B60W10/08 , A61K47/61 , A61K47/68 , A61K49/126 , A61K49/16 , B60L7/14 , B60L11/1803 , B60L2220/42 , B60L2220/50 , C08B37/0021 , C08L5/02 , G01N33/54353 , Y02T10/6217 , Y02T10/6221 , Y02T10/641 , Y02T10/648 , Y02T10/7005
摘要： This invention relates to a method of conveniently functionalizing the reducing end of a carbohydrate polymer. The method also allows for the length of the polymer to be differentially functionalized. The invention provides a method for preparing polymers with a different functional groups or functionality on the sides than on the reducing end. An advantage of the method of the invention is that the procedures are simpler, preferably requiring less time, fewer steps, reduced temperatures and less harsh chemical components than conventional procedures for differential modification, with the further advantage that the reactions can be carried out in aqueous media.
摘要翻译：本发明涉及方便官能化碳水化合物聚合物的还原末端的方法。该方法还允许聚合物的长度差异功能化。本发明提供了一种在侧面制备具有不同官能团或官能团的聚合物的方法，而不是在还原端。本发明方法的一个优点是与常规的差异修饰方法相比，方法更简单，优选需要更少的时间，更少的步骤，降低的温度和更少的苛刻的化学成分，另外的优点是反应可以在水溶液媒体。

4. 发明申请

WO2011084705A3 CHEMICAL REAGENTS FOR THE ACTIVATION OF POLYSACCHARIDES IN THE PREPARATION OF CONJUGATE VACCINES 审中-公开
标题翻译：化学试剂在多糖制剂中的活化作用
公开(公告)号：WO2011084705A3
公开(公告)日：2012-03-01
申请号：PCT/US2010061133
申请日：2010-12-17
申请人： FINA BIOSOLUTIONS LLC , LEES ANDREW
发明人： LEES ANDREW
IPC分类号： C07D211/18 , C07K17/10
CPC分类号： A61K47/4823 , A61K39/00 , A61K39/095 , A61K39/145 , A61K47/61 , A61K47/643 , A61K2039/6087 , A61K2039/627 , C08B37/0021 , C08B37/006 , C08H1/00
摘要： This invention provides novel reagents for cyanating polysaccharides in aqueous or part aqueous solutions so that they may be covalently linked to proteins either directly or through a spacer. These reagents include l-cyano-4-pyrrolidinopyridinium tetrafluoroborate (CPPT), 1-cyano-imidazole (1-CI), 1-cyanobenzotriazole (1-CBT), or 2-cyanopyridazine-3(2H)one (2-CPO), or a functional derivative or modification thereof. The examples illustrate the use of these reagents with a variety of polysaccharides and proteins showing that the methods are generally applicable.
摘要翻译：本发明提供用于在水溶液或部分水溶液中氰化多糖的新型试剂，使得它们可以直接或通过间隔物与蛋白质共价连接。这些试剂包括1-氰基-4-吡咯烷基吡啶鎓四氟硼酸盐（CPPT），1-氰基咪唑（1-CI），1-氰基苯并三唑（1-CBT）或2-氰基哒嗪-3（2H），或其功能衍生物或修饰物。这些实施例说明了这些试剂与各种多糖和蛋白质的应用，表明该方法通常是适用的。

5. 发明申请

WO2011053971A3 METHOD FOR ENHANCING THE SENSITIVITY OF ANTIBODY BASED ASSAYS 审中-公开
标题翻译：用于增强基于抗体测定的灵敏度的方法
公开(公告)号：WO2011053971A3
公开(公告)日：2011-09-15
申请号：PCT/US2010055107
申请日：2010-11-02
申请人： FINA BIOSOLUTIONS LLC , LEES ANDREW
发明人： LEES ANDREW
IPC分类号： G01N33/53 , G01N33/543 , G01N33/58
CPC分类号： G01N33/548
摘要： There is a constant need for increased sensitivity of antibody-based assays as well as a need for high affinity antibodies. Higher affinity molecules such as antibodies increase the specificity and sensitivity of assays. Both increased sensitivity and higher affinity can be achieved when multiple copies of a binding molecule are coupled to a flexible backbone. According to one embodiment of the invention, multiple copies of a detection molecule, such as, for example, an antibody or antibody fragment, are covalently linked to a high molecular weight water soluble polymer creates multivalent antibody constructs. The affinity or avidity increases markedly as multivalency increases and also, multiple copies of a signal molecule can be similarly added to the polymer. Thus, the construct comprises multiple copies of detection and signal molecules on a high molecular weight, water soluble, flexible polymer. Broadly, the invention comprises a multiplicity of detection molecules (D) and a multiplicity of signal molecules (S) on a polymer (P), such that Pmw (Dn, Sm), where n and m are greater than or equal to 2, and Pmw refers to an average molecular weight of the polymer. These molecules provide for both conventional and nonconventional assays, an increased sensitivity of antigen detection.
摘要翻译：不断需要增加基于抗体的测定的灵敏度以及需要高亲和力抗体。更高亲和力的分子如抗体增加了测定的特异性和灵敏度。当结合分子的多个拷贝与柔性骨架偶联时，可以实现提高的灵敏度和更高的亲和力。根据本发明的一个实施方案，检测分子（例如抗体或抗体片段）的多个拷贝与高分子量水溶性聚合物共价连接产生多价抗体构建体。随着多价增加，亲和力或亲合力显着增加，并且信号分子的多个拷贝可以类似地加入到聚合物中。因此，构建体包含高分子量水溶性柔性聚合物上的检测和信号分子的多个拷贝。广泛地，本发明包括多个检测分子（D）和聚合物（P）上的多个信号分子（S），使得其中n和m大于或等于2的Pmw（Dn，Sm）而Pmw是指聚合物的平均分子量。这些分子提供常规和非常规测定，提高抗原检测的灵敏度。

6. 发明专利

CA2938251C EXPRESSION AND PURIFICATION OF CRM197 AND RELATED PROTEINS 未知
公开(公告)号：CA2938251C
公开(公告)日：2021-01-12
申请号：CA2938251
申请日：2015-02-02
申请人： FINA BIOSOLUTIONS LLC
发明人： OGANESYAN NATALIA , LEES ANDREW
IPC分类号： C12N15/64 , C07K1/16 , C07K14/34 , C12N1/21 , C12N5/00 , C12N5/02 , C12N15/31 , C12N15/53 , C12N15/63 , C12P21/02 , G01N33/53
摘要： The present invention is directed to the cells, compositions and methods for the production of recombinant protein. In particular, the invention is directed to a production process for obtaining high levels of soluble recombinant CRM197 protein from E. coli. Cells preferably contain one or more mutations of disulfide reductase genes, so that disulfide reductase activity is reduced. The invention also relates to purification method for CRM197 as well as characterization of properly folded CRM197 protein.

7. 发明专利

CA2836153C CHEMICAL REAGENTS FOR THE ACTIVATION OF POLYSACCHARIDES IN THE PREPARATION OF CONJUGATE VACCINES 未知
公开(公告)号：CA2836153C
公开(公告)日：2016-06-21
申请号：CA2836153
申请日：2010-12-17
申请人： FINA BIOSOLUTIONS LLC
发明人： LEES ANDREW
IPC分类号： C07K17/10 , A61K39/385 , A61K49/00 , C07D233/58 , C07H1/00 , C07K1/107 , C07K1/14
摘要： This invention provides novel reagents for cyanating polysaccharides in aqueous or part aqueous solutions so that they may be covalently linked to proteins either directly or through a spacer. These reagents include 1-cyano-4-pyrrolidinopyridinium tetrafluoroborate (CPPT), 1-cyano-imidazole (1-CI), 1-cyanobenzotriazole (1- CBT), or 2-cyanopyridazine-3(2H)one (2-CPO), or a functional derivative or modification thereof. The examples illustrate the use of these reagents with a variety of polysaccharides and proteins showing that the methods are generally applicable.

8. 发明专利

CA2836140C CHEMICAL REAGENTS FOR THE ACTIVATION OF POLYSACCHARIDES IN THE PREPARATION OF CONJUGATE VACCINES 未知
公开(公告)号：CA2836140C
公开(公告)日：2016-06-21
申请号：CA2836140
申请日：2010-12-17
申请人： FINA BIOSOLUTIONS LLC
发明人： LEES ANDREW
IPC分类号： C07K17/10 , A61K39/385 , A61K49/00 , A61P37/04 , C07D249/18 , C07H1/00 , C07K1/107 , C07K1/14
摘要： This invention provides novel reagents for cyanating polysaccharides in aqueous or part aqueous solutions so that they may be covalently linked to proteins either directly or through a spacer. These reagents include 1-cyano-4-pyrrolidinopyridinium tetrafluoroborate (CPPT), 1-cyano-imidazole (1-CI), 1-cyanobenzotriazole (1- CBT), or 2-cyanopyridazine-3(2H)one (2-CPO), or a functional derivative or modification thereof. The examples illustrate the use of these reagents with a variety of polysaccharides and proteins showing that the methods are generally applicable.

9. 发明专利

SG10201506162RA CHEMICAL REAGENTS FOR THE ACTIVATION OF POLYSACCHARIDES IN THE PREPARATION OF CONJUGATE VACCINES 未知
公开(公告)号：SG10201506162RA
公开(公告)日：2015-09-29
申请号：SG10201506162R
申请日：2010-12-17
申请人： FINA BIOSOLUTIONS LLC
发明人： LEES ANDREW
摘要： This invention provides novel reagents for cyanating polysaccharides in aqueous or part aqueous solutions so that they may be covalently linked to proteins either directly or through a spacer. These reagents include 1-cyano-4-pyrrolidinopyridinium tetrafluoroborate (CPPT), 1-cyano-imidazole (1-CI), 1-cyanobenzotriazole (1-CBT), or 2-cyanopyridazine-3(2H)one (2-CPO), or a functional derivative or modification thereof. The examples illustrate the use of these reagents with a variety of polysaccharides and proteins showing that the methods are generally applicable.

10. 发明专利

SG10201506160VA CHEMICAL REAGENTS FOR THE ACTIVATION OF POLYSACCHARIDES IN THE PREPARATION OF CONJUGATE VACCINES 未知
公开(公告)号：SG10201506160VA
公开(公告)日：2015-09-29
申请号：SG10201506160V
申请日：2010-12-17
申请人： FINA BIOSOLUTIONS LLC
发明人： LEES ANDREW
摘要： This invention provides novel reagents for cyanating polysaccharides in aqueous or part aqueous solutions so that they may be covalently linked to proteins either directly or through a spacer. These reagents include 1-cyano-4-pyrrolidinopyridinium tetrafluoroborate (CPPT), 1-cyano-imidazole (1-CI), 1-cyanobenzotriazole (1-CBT), or 2-cyanopyridazine-3(2H)one (2-CPO), or a functional derivative or modification thereof. The examples illustrate the use of these reagents with a variety of polysaccharides and proteins showing that the methods are generally applicable.

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