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1. 发明申请

WO2014001326A1 METHOD FOR THE SELECTION AND PRODUCTION OF TAILOR-MADE, SELECTIVE AND MULTI-SPECIFIC THERAPEUTIC MOLECULES COMPRISING AT LEAST TWO DIFFERENT TARGETING ENTITIES AND USES THEREOF 审中-公开
标题翻译：用于选择和生产包含至少两个不同目标实体的选择性和选择性和多特异性治疗分子的方法及其用途
公开(公告)号：WO2014001326A1
公开(公告)日：2014-01-03
申请号：PCT/EP2013/063260
申请日：2013-06-25
申请人： F. HOFFMANN-LA ROCHE AG , HOFFMANN-LA ROCHE INC.
发明人： HEINDL, Dieter , HUELSMANN, Peter, Michael , KALUZA, Brigitte , KOPETZKI, Erhard , NIEDERFELLNER, Gerhard , TIEFENTHALER, Georg
IPC分类号： C07K16/00 , C07K16/46 , A61K39/395
CPC分类号： C07K16/00 , C07K16/32 , C07K16/468 , C12Q1/26 , C12Q1/58 , C12Q1/66 , G01N33/53 , G01N33/563
摘要： Herein is reported a method for determining a combination of antigen binding sites comprising the steps of (i) determining the binding specificity and/or affinity and/or effector function and/or in vivo half-life of a multitude of bispecific antibodies prepared by combining each member of a first multitude of antibody Fab fragments or scFv antibody fragments with each member of a second multitude of antibody Fab fragments or scFv antibody fragments, and a linker comprising at one of its termini the second member of the first binding pair and at the respective other terminus the second member of the second binding pair, whereby the first multitude specifically binds to a first cell surface molecule and the second multitude specifically binds to a second cell surface molecule, and (ii) choosing the bispecific antibody with suitable binding specificity and/or affinity and/or effector function and/or in vivo half-life and thereby determining a combination of antigen binding sites.
摘要翻译：本文报道了用于确定抗原结合位点的组合的方法，包括以下步骤：（i）测定通过组合制备的大量双特异性抗体的结合特异性和/或亲和力和/或效应子功能和/或体内半衰期每个成员具有第二大量抗体Fab片段或scFv抗体片段的每个成员的第一大量抗体Fab片段或scFv抗体片段，以及在其末端包含第一结合对的第二个成员和相应的另一个末端是第二结合对的第二个成员，其中第一个人特异性结合第一细胞表面分子，第二个人特异性结合第二细胞表面分子，和（ii）选择具有合适结合特异性的双特异性抗体， /或亲和力和/或效应子功能和/或体内半衰期，从而确定抗原结合位点的组合。

2. 发明申请

WO2016096787A1 ENZYMATIC ONE-POT REACTION FOR DOUBLE POLYPEPTIDE CONJUGATION IN A SINGLE STEP USING SORTASE 审中-公开
标题翻译：在使用筛选的单一步骤中双酚聚糖的酶一反应反应
公开(公告)号：WO2016096787A1
公开(公告)日：2016-06-23
申请号：PCT/EP2015/079692
申请日：2015-12-15
申请人： F. HOFFMANN-LA ROCHE AG , HOFFMANN-LA ROCHE INC.
发明人： HEINDL, Dieter , KOPETZKI, Erhard , TIEFENTHALER, Georg
IPC分类号： C07K16/00 , C07K16/32 , C07K16/46 , C12N9/52
CPC分类号： C12P21/02 , C07K16/00 , C07K16/32 , C07K16/468 , C07K2317/24 , C07K2317/40 , C07K2317/52 , C07K2317/55 , C07K2317/64 , C12Y304/2207
摘要： Herein is reported a method for producing an enzymatic conjugation product of three polypeptides comprising the simultaneous incubation of i) a first polypeptide comprising the amino acid sequence LPXTG (SEQ ID NO: 20, wherein X can be any amino acid residue), a second polypeptide comprising the amino acid sequence LPXTA (SEQ ID NO: 31, wherein X can be any amino acid residue), a third polypeptide that has two N-termini whereby the polypeptide has an oligo-glycine G m (m = 2 (SEQ ID NO: 22), or 3 (SEQ ID NO: 23), or 4 (SEQ ID NO: 24), or 5 (SEQ ID NO: 25)) amino acid sequence at its first N-terminus and an oligo-alanine A m (m = 2 (SEQ ID NO: 26), or 3 (SEQ ID NO: 27), or 4 (SEQ ID NO: 28), or 5 (SEQ ID NO: 29)) amino acid sequence at its second N-terminus, a fourth polypeptide with sortase activity whereby the polypeptide is derived from Staphylococcus aureus sortase A, and a fifth polypeptide with sortase activity whereby the polypeptide is derived from Streptococcus pyogenes sortase A and the recovering of the conjugate from the reaction mixture.
摘要翻译：本文报道了一种用于产生三种多肽的酶联结产物的方法，包括同时孵育i）包含氨基酸序列LPXTG（SEQ ID NO：20，其中X可以是任何氨基酸残基）的第一多肽，第二多肽包括氨基酸序列LPXTA（SEQ ID NO：31，其中X可以是任何氨基酸残基），具有两个N-末端的第三多肽，由此多肽具有寡甘氨酸Gm（m = 2（SEQ ID NO： 22）或3（SEQ ID NO：23）或4（SEQ ID NO：24）或5（SEQ ID NO：25））氨基酸序列和低聚丙氨酸Am（m = 2（SEQ ID NO：26）或3（SEQ ID NO：27）或4（SEQ ID NO：28）或5（SEQ ID NO：29））氨基酸序列，具有分选酶活性的第四多肽，其中所述多肽衍生自金黄色葡萄球菌分选酶A，以及具有分选酶活性的第五多肽，其中多肽衍生自化脓性链球菌分选酶A 和从反应混合物中回收共轭物。

3. 发明申请

WO2022263489A1 NUCLEOSIDE-5 -OLIGOPHOSPHATES HAVING A CATIONICALLY-MODIFIED NUCLEOBASE 审中-公开
公开(公告)号：WO2022263489A1
公开(公告)日：2022-12-22
申请号：PCT/EP2022/066263
申请日：2022-06-15
申请人： F. HOFFMANN-LA ROCHE AG , ROCHE DIAGNOSTICS GMBH , ROCHE SEQUENCING SOLUTIONS, INC.
发明人： CRISALLI, Peter , HEINDL, Dieter , KHAKSHOOR, Omid , KUCHELMEISTER, Hannes , MEX, Martin , TAING, Meng C.
IPC分类号： C07H19/10 , C07H21/04 , B82B3/00 , B82Y5/00 , C12Q1/6869
摘要： Disclosed herein are base-modified nucleoside-5 '-oligophosphates (bm-N5OP) that include a positively charged moiety at least at one position of the base, compositions comprising the same, compositions made from the same, methods of making the same, and methods of using the same. The bm-N50P disclosed herein are useful, for example, as tagged nucleotides for use in nanoSBS methods and for generating primers and/or templates for use in nanoSBS methods, When incorporated into a polynucleotide, the disclosed bm-N50Ps can neutralize at least a portion of the negative charge of the overall polynucleotide molecule.

4. 发明申请

WO2020127620A1 DETECTION OF TARGET NUCLEIC ACID BY SOLID-PHASE MOLOGRAPHY 审中-公开
公开(公告)号：WO2020127620A1
公开(公告)日：2020-06-25
申请号：PCT/EP2019/086145
申请日：2019-12-19
申请人： F. HOFFMANN-LA ROCHE AG , ROCHE DIAGNOSTICS GMBH , ROCHE MOLECULAR SYSTEMS, INC.
发明人： HEINDL, Dieter , STENGELE, Nikolaus-Peter , TSAN, Alison , KOZLOV, Igor , GATTERDAM, Volker
IPC分类号： C12Q1/6825 , C12Q1/6853
摘要： The present invention describes methods for performing real-time PCR for detection and quantitation of target nucleic acids using tagged oligonucleotide probes and solid-phase molography.

5. 发明申请

WO2020020850A1 REAGENT FOR MASS SPECTROMETRY 审中-公开
公开(公告)号：WO2020020850A1
公开(公告)日：2020-01-30
申请号：PCT/EP2019/069730
申请日：2019-07-23
申请人： F. HOFFMANN-LA ROCHE AG , ROCHE DIAGNOSTICS GMBH , ROCHE DIAGNOSTICS OPERATIONS, INC.
发明人： BAECHER, Silvia , GEIERMANN, Anna-Skrollan , HEINDL, Dieter , JOSEL, Hans-Peter , KOBOLD, Uwe , LEINENBACH, Andreas , LOIBL, Simon Ferdinand , MILIC, Jelena , PIRKL, Nicole , PRENCIPE, Giuseppe , REMPT, Martin , SEIDEL, Christoph
IPC分类号： C07D249/06 , C07D257/04
摘要： The present invention relates to reagents which are suitable to be used in mass spectrometry as well as methods of mass spectrometric determination of analyte molecules using said reagents.

6. 发明申请

WO2017109077A1 COMPOSITIONS WITH THERMOLABILE DYES 审中-公开
标题翻译：热可塑性染料的组合物
公开(公告)号：WO2017109077A1
公开(公告)日：2017-06-29
申请号：PCT/EP2016/082391
申请日：2016-12-22
申请人： ROCHE DIAGNOSTICS GMBH , F. HOFFMANN-LA ROCHE AG , ROCHE MOLECULAR SYSTEMS, INC.
发明人： HEINDL, Dieter , KIRZINGER, Hannes , MEIER, Thomas , NELSON, Christopher
IPC分类号： C07K14/435 , C09K11/07 , C12Q1/68
CPC分类号： C12Q1/68 , C09K11/07 , C12N9/1241 , C12Q1/686 , C12Q2527/101 , C12Q2527/127 , C12Q2531/113 , C12Q2563/107 , C12Q2563/119
摘要： The present disclosure provides an aqueous composition comprising a purified thermostable polypeptide with biological activity, and a purified thermolabile water-soluble substance which in a functional conformation at a permissive temperature absorbs light and/or exhibits fluorescence, wherein the maximal permissive temperature of the polypeptide is above the maximal permissive temperature of the substance. The present disclosure further provides methods employing such a composition, and kits containing ingredients to form such a composition.
摘要翻译：本公开提供了包含具有生物活性的纯化的热稳定性多肽和纯化的不耐热水溶性物质的水性组合物，所述物质在允许温度下以功能性构象吸收光和/或显示荧光，其中多肽的最大允许温度高于该物质的最大允许温度。本公开进一步提供了使用这种组合物的方法和包含成分以形成这种组合物的试剂盒。

7. 发明申请

WO2015128157A1 METHOD OF IDENTIFYING A NUCLEOTIDE AT A DEFINED POSITION AND DETERMINING THE SEQUENCE OF A TARGET POLYNUCLEOTIDE USING AN ELECTRO-SWITCHABLE BIOSENSOR, AS WELL AS DEVICES COMPRISING AN ELECTRO-SWITCHABLE BIOSENSOR AND USES THEREOF 审中-公开
标题翻译：在定义的位置鉴定核苷酸的方法和使用电可切换生物传感器确定目标多核苷酸的序列，以及作为包含电可切换生物传感器的装置及其用途
公开(公告)号：WO2015128157A1
公开(公告)日：2015-09-03
申请号：PCT/EP2015/052131
申请日：2015-02-03
申请人： ROCHE DIAGNOSTICS GMBH , F. HOFFMANN-LA ROCHE AG , ROCHE DIAGNOSTICS OPERATIONS, INC. , DYNAMIC BIOSENSORS GMBH
发明人： LANGER, Andreas , RANT, Ulrich , STRASSER, Ralf , HEINDL, Dieter , SCHRAEML, Michael
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , G01N27/3276 , C12Q2533/101 , C12Q2563/107 , C12Q2563/116 , C12Q2565/607
摘要： The present invention relates to a method of identifying a nucleotide at a defined position and determining the sequence of a target polynucleotide using an electro-switchable biosensor, as well as devices comprising an electro-switchable biosensor and uses thereof.
摘要翻译：本发明涉及一种在确定的位置鉴定核苷酸并使用可电切换生物传感器确定靶多核苷酸的序列的方法，以及包括可电切换生物传感器及其用途的装置。

8. 发明申请

WO2012101095A1 METHOD AND SUBSTANCES FOR PREPARATION OF N-SUBSTITUTED PYRIDINIUM COMPOUNDS 审中-公开
标题翻译：制备N-取代的吡啶鎓化合物的方法和物质
公开(公告)号：WO2012101095A1
公开(公告)日：2012-08-02
申请号：PCT/EP2012/050996
申请日：2012-01-24
申请人： ROCHE DIAGNOSTICS GMBH , F. HOFFMANN-LA ROCHE AG , GEBAUER, Peter , HEINDL, Dieter , HORN, Carina
发明人： GEBAUER, Peter , HEINDL, Dieter , HORN, Carina
IPC分类号： C07D213/46
CPC分类号： C07F9/58 , C07C251/12 , C07D213/46
摘要： The present invention relates to a method for the synthesis of N-substituted 3- acylated pyridinium compounds by reacting a pentamethine precursor with a primary amine.
摘要翻译：本发明涉及通过使五甲胺前体与伯胺反应来合成N-取代的3-酰化吡啶鎓化合物的方法。

9. 发明申请

WO2012085113A1 BINDING AGENT 审中-公开
标题翻译：绑定代理
公开(公告)号：WO2012085113A1
公开(公告)日：2012-06-28
申请号：PCT/EP2011/073633
申请日：2011-12-21
申请人： ROCHE DIAGNOSTICS GMBH , F. HOFFMANN-LA ROCHE AG , GALLUSSER, Andreas , HEINDL, Dieter , SCHRAEML, Michael , SEIDEL, Christoph , VON DER ELTZ, Herbert
发明人： GALLUSSER, Andreas , HEINDL, Dieter , SCHRAEML, Michael , SEIDEL, Christoph , VON DER ELTZ, Herbert
IPC分类号： C07K16/00 , C07K16/18
CPC分类号： C07K19/00 , C07K16/18 , C07K16/2863 , C07K16/32 , C07K2317/31 , C07K2317/34 , C07K2317/55 , C07K2317/626 , C07K2317/92 , C07K2317/94
摘要： The present invention relates to a binding agent of the FormulaA - a':a - S- b:b' - B:X(n), wherein A as well as B is a monovalent binder, wherein a':a as well as b:b' is a binding pair wherein a' and a do not interfere with the binding of b to b' and vice versa, wherein S is a spacer of at least 1 nm in length, wherein :X denotes a functional moiety bound either covalently or via a binding pair to at least one of a', a, b, b' or S, wherein (n) is an integer and at least 1, wherein - represents a covalent bond, and wherein the linker a - S - b has a length of 6 to 100 nm. Also disclosed are a method of producing such binding agent and certain uses thereof.
摘要翻译：本发明涉及式A-a'：a-S-b：b'-B：X（n）的结合剂，其中A以及B是单价粘合剂，其中a： b：b'是一个结合对，其中a'和a不干扰b与b'的结合，反之亦然，其中S是长度至少为1nm的间隔物，其中：X表示与共价或经由与a'，a，b，b'或S中的至少一个的结合对，其中（n）为整数且至少为1，其中 - 表示共价键，并且其中所述连接体a-S- b的长度为6〜100nm。还公开了生产这种结合剂及其某些用途的方法。

10. 发明申请

WO2011012271A2 METHOD AND SUBSTANCES FOR PREPARATION OF N-SUBSTITUTED PYRIDINIUM COMPOUNDS 审中-公开
标题翻译：制备N-取代的吡啶鎓化合物的方法和物质
公开(公告)号：WO2011012271A2
公开(公告)日：2011-02-03
申请号：PCT/EP2010/004524
申请日：2010-07-23
申请人： ROCHE DIAGNOSTICS GMBH , F. HOFFMANN-LA ROCHE AG , HEINDL, Dieter , GEBAUER, Peter
发明人： HEINDL, Dieter , GEBAUER, Peter
IPC分类号： C07D213/80 , C07D213/803
CPC分类号： C07D213/803 , C07D213/80
摘要： The present invention relates to a method for the synthesis of N-substituted carboxylated pyridinium compounds by reacting a pentamethine precursor with a primary amine. In this reaction an N-substituted alkoxycarbonyl pyridinium heterocycle is formed.
摘要翻译：本发明涉及通过使五甲胺前体与伯胺反应来合成N-取代的羧化吡啶鎓化合物的方法。在该反应中，形成N-取代的烷氧基羰基吡啶鎓杂环。

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