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2. 发明授权

US08062861B2 Tissue sample preprocessing methods and devices 有权
标题翻译：组织样品预处理方法和装置
公开(公告)号：US08062861B2
公开(公告)日：2011-11-22
申请号：US12389154
申请日：2009-02-19
申请人： Eswar Prasad Ramachandran Iyer , Daniel N. Cox
发明人： Eswar Prasad Ramachandran Iyer , Daniel N. Cox
IPC分类号： G01N1/30
CPC分类号： G01N1/36 , C12M45/02 , G01N2001/362
摘要： To preprocess a tissue sample, a first permeable mesh membrane is tautly stretched out over the tissue sample and overlaid on top of a flat surface. This covering sandwiches and secures the tissue sample in between the first permeable mesh membrane and the flat surface. If it is desired to reinforce the mechanical strength of the first permeable mesh membrane, a second permeable mesh membrane can be tautly stretched out and overlaid on top of the sandwich. Alternatively, the second permeable mesh membrane can be attached onto a third component, such as a disposable solvent resistant frame, to create a framed mesh. Thereafter, the first permeable mesh membrane may be attached to the framed mesh to create a compound framed mesh. The tissue sample may then be sandwiched and secured in between the compound framed mesh and the flat surface. To retain and flatten the tissue sample, gentle pressure may be uniformly applied.
摘要翻译：为了预处理组织样品，将第一可渗透网膜张紧地伸展到组织样品上并且覆盖在平坦表面的顶部上。这种覆盖三明治并将组织样品固定在第一可渗透网膜和平坦表面之间。如果希望增强第一可渗透网膜的机械强度，则可以将第二可渗透网膜张紧地拉伸并覆盖在夹层的顶部上。或者，第二可渗透网膜可以附接到第三部件，例如一次性耐溶剂框架上，以形成框架网。此后，第一可渗透网膜可以附接到框架网以产生复合框架网。然后可以将组织样品夹在并固定在复合框架网和平坦表面之间。为了保持和平坦化组织样品，可以均匀地施加温和的压力。

3. 发明授权

US08778630B2 Tissue sample preprocessing methods and devices 有权
标题翻译：组织样品预处理方法和装置
公开(公告)号：US08778630B2
公开(公告)日：2014-07-15
申请号：US13267105
申请日：2011-10-06
申请人： Eswar Prasad Ramachandran Iyer , Daniel N. Cox
发明人： Eswar Prasad Ramachandran Iyer , Daniel N. Cox
IPC分类号： G01N1/30
CPC分类号： G01N1/36 , C12M45/02 , G01N2001/362
摘要： A first permeable mesh membrane may be configured to be tautly stretched over a tissue sample and overlaid over a surface. A first permeable mesh membrane may sandwich and secure a tissue sample in between a first permeable mesh membrane and a surface. A first permeable mesh membrane may have a sieve size of approximately 10 μm to approximately 100 μm.
摘要翻译：可以将第一可渗透网膜构造成在组织样品上张紧拉伸并覆盖在表面上。第一可渗透网膜可以将组织样本夹在并固定在第一可渗透网膜和表面之间。第一可渗透网膜可以具有约10μm至约100μm的筛子尺寸。

4. 发明申请

US20120088267A1 Tissue Sample Preprocessing Methods and Devices 有权
标题翻译：组织样品预处理方法和装置
公开(公告)号：US20120088267A1
公开(公告)日：2012-04-12
申请号：US13267105
申请日：2011-10-06
申请人： Eswar Prasad Ramachandran Iyer , Daniel N. Cox
发明人： Eswar Prasad Ramachandran Iyer , Daniel N. Cox
IPC分类号： G01N1/28 , B01L99/00
CPC分类号： G01N1/36 , C12M45/02 , G01N2001/362
摘要： A first permeable mesh membrane may be configured to be tautly stretched over a tissue sample and overlaid over a surface. A first permeable mesh membrane may sandwich and secure a tissue sample in between a first permeable mesh membrane and a surface. A first permeable mesh membrane may have a sieve size of approximately 10 μm to approximately 100 μm.
摘要翻译：可以将第一可渗透网膜构造成在组织样品上张紧拉伸并覆盖在表面上。第一可渗透网膜可以将组织样本夹在并固定在第一可渗透网膜和表面之间。第一可渗透网膜可以具有约10μm至约100μm的筛子尺寸。

5. 发明申请

US20090215112A1 Tissue Sample Preprocessing Methods and Devices 有权
标题翻译：组织样品预处理方法和装置
公开(公告)号：US20090215112A1
公开(公告)日：2009-08-27
申请号：US12389154
申请日：2009-02-19
申请人： Eswar Prasad Ramachandran Iyer , Daniel N. Cox
发明人： Eswar Prasad Ramachandran Iyer , Daniel N. Cox
IPC分类号： G01N33/48 , C12M1/00
CPC分类号： G01N1/36 , C12M45/02 , G01N2001/362
摘要： To preprocess a tissue sample, a first permeable mesh membrane is tautly stretched out over the tissue sample and overlaid on top of a flat surface. This covering sandwiches and secures the tissue sample in between the first permeable mesh membrane and the flat surface. If it is desired to reinforce the mechanical strength of the first permeable mesh membrane, a second permeable mesh membrane can be tautly stretched out and overlaid on top of the sandwich. Alternatively, the second permeable mesh membrane can be attached onto a third component, such as a disposable solvent resistant frame, to create a framed mesh. Thereafter, the first permeable mesh membrane may be attached to the framed mesh to create a compound framed mesh. The tissue sample may then be sandwiched and secured in between the compound framed mesh and the flat surface. To retain and flatten the tissue sample, gentle pressure may be uniformly applied.
摘要翻译：为了预处理组织样品，将第一可渗透网膜张紧地伸展到组织样品上并覆盖在平坦表面的顶部上。这种覆盖三明治并将组织样品固定在第一可渗透网膜和平坦表面之间。如果希望增强第一可渗透网膜的机械强度，则可以将第二可渗透网膜张紧地拉伸并覆盖在夹层的顶部上。或者，第二可渗透网膜可以附接到第三部件，例如一次性耐溶剂框架上，以形成框架网。此后，第一可渗透网膜可以附接到框架网以产生复合框架网。然后可以将组织样品夹在并固定在复合框架网和平坦表面之间。为了保持和平坦化组织样品，可以均匀地施加温和的压力。

6. 发明公开

US20240287600A1 METHODS FOR DETERMINING A LOCATION OF A BIOLOGICAL ANALYTE IN A BIOLOGICAL SAMPLE 审中-公开
公开(公告)号：US20240287600A1
公开(公告)日：2024-08-29
申请号：US18596867
申请日：2024-03-06
申请人： 10x Genomics, Inc.
发明人： Eswar Prasad Ramachandran Iyer , Tarjei Sigurd Mikkelsen
IPC分类号： C12Q1/6874 , C12Q1/6841
CPC分类号： C12Q1/6874 , C12Q1/6841 , C12Q2525/161 , C12Q2565/519
摘要： Provided herein are methods of determining a location of a biological analyte in a biological sample.

7. 发明申请

US20220119869A1 METHODS FOR DETERMINING A LOCATION OF AN ANALYTE IN A BIOLOGICAL SAMPLE 有权
公开(公告)号：US20220119869A1
公开(公告)日：2022-04-21
申请号：US17565081
申请日：2021-12-29
申请人： 10x Genomics, Inc.
发明人： Eswar Prasad Ramachandran Iyer , Zachary Bent , Yifeng Yin , Eileen Dalin
IPC分类号： C12Q1/6837 , C12Q1/6841
摘要： Provided herein are methods of determining a location of a target analyte in a non-permeabilized biological sample that include the use of a blocking probe.

8. 发明申请

US20210317524A1 RESOLVING SPATIAL ARRAYS 有权
公开(公告)号：US20210317524A1
公开(公告)日：2021-10-14
申请号：US17272033
申请日：2019-08-27
申请人： 10x Genomics, Inc.
发明人： Michael Ybarra Lucero , Michael Schnall-Levin , Tarjei Sigurd Mikkelsen , Eswar Prasad Ramachandran Iyer , Paul Giresi , Benjamin Hindson
IPC分类号： C12Q1/6874
摘要： Methods for determining a location of a feature in a spatial array of features include: providing a spatial array including a plurality of features on a substrate, where a feature of the plurality of features has a capture probe, and where the capture probe has a spatial barcode and a constant sequence; hybridizing a first sequencing probe to the spatial barcode, where the first sequencing probe has a first label and a first nucleotide sequence; obtaining a first image of the first label with the first sequencing probe hybridized to the spatial barcode; determining, based on the first image, a first portion of a sequence of the spatial barcode; and associating the feature with a location in the spatial array based on a location of the first label in the first image.

9. 发明授权

US11891654B2 Methods of making gene expression libraries 有权
公开(公告)号：US11891654B2
公开(公告)日：2024-02-06
申请号：US17184117
申请日：2021-02-24
申请人： 10x Genomics, Inc.
发明人： Luigi Jhon Alvarado Martinez , Eswar Prasad Ramachandran Iyer
IPC分类号： C12Q1/6837 , C12Q1/6816 , C12Q1/6827
CPC分类号： C12Q1/6837 , C12Q1/6816 , C12Q1/6827 , C12Q2543/101 , C12Q2565/514
摘要： Provided herein are methods of determining a location of a target nucleic acid in a biological sample.

10. 发明授权

US11827935B1 Methods for spatial analysis using rolling circle amplification and detection probes 有权
公开(公告)号：US11827935B1
公开(公告)日：2023-11-28
申请号：US17531093
申请日：2021-11-19
申请人： 10x Genomics, Inc.
发明人： Eswar Prasad Ramachandran Iyer , Yifeng Yin , Sultan Doganay Tuncer
IPC分类号： C12Q1/6874 , C12Q1/6855 , C12Q1/6862
CPC分类号： C12Q1/6874 , C12Q1/6855 , C12Q1/6862
摘要： Provided herein are methods of improving sensitivity of spatial detection of an analyte in a biological sample using splint oligonucleotides, circularized second strands, and rolling circle amplification. For example, provided herein are methods of improving sensitivity of spatial detection of an analyte in a biological sample where a splint oligonucleotide hybridizes to a second strand; the second strand is ligated together thereby creating a circularized second strand, rolling circle amplification of the circularized second strand results in generation of an amplified second strand, and all or part of the sequence of the amplified second strand is determined and used to spatially detect the analyte in the biological sample.

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