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1. 发明授权

US4544631A Process and reagent for the determination of .alpha.-amylase 失效
标题翻译：用于测定α-淀粉酶的方法和试剂
公开(公告)号：US4544631A
公开(公告)日：1985-10-01
申请号：US311856
申请日：1981-10-16
申请人： Elli Rauscher , Ulrich Neumann , August W. Wahlefeld , Alexander Hagen , Wolfgang Gruber , Joachim Ziegenhorn , Eugen Schaich , Ulfert Deneke , Gerhard Michal , Gunter Weimann
发明人： Elli Rauscher , Ulrich Neumann , August W. Wahlefeld , Alexander Hagen , Wolfgang Gruber , Joachim Ziegenhorn , Eugen Schaich , Ulfert Deneke , Gerhard Michal , Gunter Weimann
IPC分类号： G01N33/50 , C07H15/04 , C07H15/203 , C12Q1/40 , C12Q1/54 , C12Q1/32 , C12Q1/34 , C12Q1/48
CPC分类号： C12Q1/40 , C12Q2334/10 , Y10S435/805 , Y10S435/81
摘要： .alpha.-Amylase is determined by the enzymatic splitting of an .alpha.-amylase substrate and measurement of a fission product, wherein there is used as a substrate a maltoheptaose compound of the formula ##STR1## wherein R is a glucoside, phenylglucoside, mononitrophenylglucoside, dinitrophenylglucoside, sorbitol or gluconic acid group. Reagents comprising such a substrate and a system for the determination of a fission product formed from the amylase substrate by .alpha.-amylase, are also provided.
摘要翻译： α-淀粉酶通过α-淀粉酶底物的酶裂解和裂变产物的测定来确定，其中用作底物的麦芽麦角糖化合物，其中R是葡萄糖苷，苯基葡糖苷，单硝基苯基葡糖苷，二硝基苯基葡糖苷，山梨糖醇或葡萄糖酸基团。还提供了包含这种底物的试剂和用于通过α-淀粉酶测定由淀粉酶底物形成的裂变产物的系统。

2. 发明授权

US5108913A Process for preparing a maltoheptaose derivative 失效
标题翻译：制备MALTOHEPTAOSE衍生物的方法
公开(公告)号：US5108913A
公开(公告)日：1992-04-28
申请号：US545092
申请日：1990-06-27
申请人： Elli Rauscher , Ulrich Neumann , August W. Wahlefeld , Alexander Hagen , Wolfgang Gruber , Joachim Ziegenhorn , Eugen Schaich , Ulfert Deneke , Michal Gerhard , Gunter Weimann
发明人： Elli Rauscher , Ulrich Neumann , August W. Wahlefeld , Alexander Hagen , Wolfgang Gruber , Joachim Ziegenhorn , Eugen Schaich , Ulfert Deneke , Michal Gerhard , Gunter Weimann
IPC分类号： G01N33/50 , C07H15/04 , C07H15/203 , C12Q1/40
CPC分类号： C12Q1/40 , C12Q2334/10 , Y10S435/805 , Y10S435/81
摘要： .alpha.-Amylase is determined by the enzymatic splitting of an .alpha.-amylase substrate and measurement of a fission product, wherein there is used as a substrate a maltoheptaose compound of the formula ##STR1## wherein R is a glucoside, phenylglucoside, mononitrophenylglucoside, dinitrophenylglucoside, sorbitol or gluconic acid group. Reagents comprising such a substrate and a system for the determination of a fission product formed from the amylase substrate by .alpha.-amylase, are also provided. A process for the preparation of a maltoheptaose will Bacillus macerans amylase, free of p-nitrophenyl-alpha-glucoside splitting activity is disclosed.

3. 发明授权

US4337310A Method for the determination of .alpha.-amylase 失效
标题翻译：测定α-淀粉酶的方法
公开(公告)号：US4337310A
公开(公告)日：1982-06-29
申请号：US184675
申请日：1980-09-08
申请人： Hans-Georg Batz , Elli Rauscher , Gunter Weimann , August W. Wahlefeld , Wolfgang Gruber
发明人： Hans-Georg Batz , Elli Rauscher , Gunter Weimann , August W. Wahlefeld , Wolfgang Gruber
IPC分类号： G01N33/50 , C08B31/00 , C08B33/00 , C12Q1/40
CPC分类号： C08B31/003 , C12Q1/40
摘要： Method for determining .alpha.-amylase by incubating with a starch substrate, wherein the substrate is based upon particulate starch derivatized with detectable groups for the determination of .alpha.-amylase, the substrate consisting of superficially cross-linked starch or amylose grains having a regulated, reduced swellability, said grains having a diameter of from 0.01 to 0.20 mm; these grains are prepared by suspending non-swollen grains in a solution with only enough cross-linking agent to achieve superficial cross-linking and then derivatizing the grains with the detectable groups for the determination of .alpha.-amylase.
摘要翻译：通过与淀粉底物孵育来测定α-淀粉酶的方法，其中所述底物是基于用可检测的基团衍生的用于测定α-淀粉酶的颗粒淀粉，所述底物由表面交联的淀粉或直链淀粉颗粒组成，所述淀粉或直链淀粉颗粒具有调节的，所述颗粒具有0.01至0.20mm的直径; 这些颗粒通过将非溶胀颗粒悬浮在仅具有足够交联剂的溶液中以实现表面交联，然后用可检测的基团衍生化以测定α-淀粉酶来制备。

4. 发明授权

US4709020A Heptaose compounds and preparation thereof 失效
标题翻译：七倍子化合物及其制备
公开(公告)号：US4709020A
公开(公告)日：1987-11-24
申请号：US637517
申请日：1984-08-03
申请人： Elli Rauscher , Eugen Schaich , Ulrich Neumann , August Wahlefeld , Wolfgang Gruber , Bernhard Empl
发明人： Elli Rauscher , Eugen Schaich , Ulrich Neumann , August Wahlefeld , Wolfgang Gruber , Bernhard Empl
IPC分类号： G01N33/50 , C07H3/06 , C07H15/04 , C07H15/203 , C07H21/00 , C08B37/00 , C12Q1/40
CPC分类号： C07H21/00 , C07H3/06 , C12Q1/40 , C12Q2334/10 , Y10S435/81
摘要： The present invention provides compounds of the general formula: ##STR1## in which R and R.sub.1, independently of one another, each represent a straight-chained or branched alkyl or alkoyl radical containing up to 6 carbon atoms or a phenyl radical or R and R.sub.1 together also form a methylene bridge, the hydrogen atoms of which, independently of one another, can each be substituted by an alkyl radical containing up to 5 carbon atoms or a phenyl radical, R.sub.2 represents an oligoglucoside residue containing 2 to 7 glucose units and X is a hydrogen atom or an optically-determinable residue.The present invention also provides a process for the preparation of these compounds.
摘要翻译：本发明提供以下通式的化合物：其中R和R 1彼此独立地表示含有至多6个碳原子的直链或支链烷基或烷酰基或苯基或 R和R1一起也形成亚甲基桥，其氢原子彼此独立地各自被含有至多5个碳原子的烷基或苯基取代，R2代表含有2至7个葡萄糖的低聚葡萄糖苷残基单元，X是氢原子或光学上可确定的残基。本发明还提供了制备这些化合物的方法。

5. 发明授权

US4818692A Method and reagent compositions for determining alpha amylase using a blocked and labeled substrate 失效
标题翻译：使用封闭和标记的底物测定α淀粉酶的方法和试剂组合物
公开(公告)号：US4818692A
公开(公告)日：1989-04-04
申请号：US68612
申请日：1987-07-01
申请人： Elli Rauscher , Eugen Schaich , Ulrich Neumann , August Wahlefeld , Wolfgang Gruber , Bernhard Empl
发明人： Elli Rauscher , Eugen Schaich , Ulrich Neumann , August Wahlefeld , Wolfgang Gruber , Bernhard Empl
IPC分类号： G01N33/50 , C07H3/06 , C07H15/04 , C07H15/203 , C07H21/00 , C08B37/00 , C12Q1/40 , C12Q1/34
CPC分类号： C07H21/00 , C07H3/06 , C12Q1/40 , C12Q2334/10 , Y10S435/81
摘要： The present invention provides compounds of the general formula: ##STR1## in which R and R.sub.1, independently of one another, each represent a straight-chained or branched alkyl or alkoyl radical containing up to 6 carbon atoms or a phenyl radical or R and R.sub.1 together also form a methylene bridge, the hydrogen atoms of which, independently of one another, can each be substituted by an alkyl radical containing up to 5 carbon atoms or a phenyl radical, R.sub.2 represents an oligoglucoside residue containing 2 to 7 glucose units and X is a hydrogen atom or an optically-determinable residue.The present invention also provides a process for the preparation of these compounds.
摘要翻译：本发明提供以下通式的化合物：其中R和R 1彼此独立地表示含有至多6个碳原子的直链或支链烷基或烷酰基或苯基或 R和R1一起也形成亚甲基桥，其氢原子彼此独立地各自被含有至多5个碳原子的烷基或苯基取代，R2代表含有2至7个葡萄糖的低聚葡萄糖苷残基单元，X是氢原子或光学上可确定的残基。本发明还提供了制备这些化合物的方法。

6. 发明授权

US4066508A Process and reagent for determining triglycerides 失效
标题翻译：用于测定TRIGLYCERIDES的方法和试剂
公开(公告)号：US4066508A
公开(公告)日：1978-01-03
申请号：US707455
申请日：1976-07-21
申请人： Elli Rauscher , Erich Bernt , Wolfgang Gruber , Helmut Determann
发明人： Elli Rauscher , Erich Bernt , Wolfgang Gruber , Helmut Determann
IPC分类号： C12N9/20 , C11C1/04 , C12Q1/02 , C12Q1/44 , C12Q1/48 , C12Q1/61 , G01N33/53 , C12K1/00
CPC分类号： C12Q1/48 , C12Q1/44 , C12Q1/61 , Y10S435/939
摘要： Triglycerides are determined by enzymatic saponification with lipase in a buffer in the presence of a carboxylesterase and an alkali metal or alkaline earth metal (10-15 carbon atom) alkyl sulfate, in the additional presence of a compound of the general formula RO(CH.sub.2 CH.sub.2).sub.n H, in which R is alkyl or alkenyl containing 14 to 20 carbon atoms and n is a number of from 7 to 20; this determination prevents turbidity and does not disadvantageously influence enzyme activity.

7. 发明授权

US5654163A Indophenol substituted maltooligosides as .alpha.-amylase substrates 失效
标题翻译：靛酚取代的麦芽糖苷作为α-淀粉酶底物
公开(公告)号：US5654163A
公开(公告)日：1997-08-05
申请号：US506261
申请日：1995-07-24
申请人： Martina Junius-Comer , Axel Schmidt , Elli Rauscher
发明人： Martina Junius-Comer , Axel Schmidt , Elli Rauscher
IPC分类号： C07H15/203 , C12Q1/40 , C07H1/00 , C12N9/26 , C12Q1/54
CPC分类号： C07H15/203 , C12Q1/40 , C12Q2334/00
摘要： The invention concerns new indophenyl-substituted maltose derivatives, their use for the determination of the enzymatic activity of .alpha.-amylase and reagents for the determination of .alpha.-amylase which contain the new indophenyl-substituted maltose derivatives as the enzyme substrate.
摘要翻译：本发明涉及新的吲哚基取代的麦芽糖衍生物，它们用于测定α-淀粉酶的酶活性和用于测定含有新的取代的取代的取代的麦芽糖衍生物作为酶底物的α-淀粉酶的试剂。

8. 发明授权

US4769447A Gamma-Glutamyl-4-azoanilides 失效
标题翻译： γ-谷氨酰基-4-偶氮酰苯胺
公开(公告)号：US4769447A
公开(公告)日：1988-09-06
申请号：US7077
申请日：1987-01-27
申请人： Elli Rauscher , John Griffiths , Leonard F. Dixon
发明人： Elli Rauscher , John Griffiths , Leonard F. Dixon
IPC分类号： C07D333/26 , C07C67/00 , C07C241/00 , C07C245/08 , C07C253/00 , C07C255/58 , C07D277/20 , C07D277/50 , C07D277/82 , C07D285/12 , C07D285/135 , C07D333/38 , C09B29/085 , C09B29/09 , C12Q1/48 , C07C107/06 , C09B43/12 , C09B43/136 , G01N31/00
CPC分类号： C12Q1/48 , Y10S435/81 , Y10T436/11
摘要： The present invention provides gamma-glutamyl-4-azoanilides, processes for their preparation and their use as substrates for gamma-glutamyl transferase determination, of the formula ##STR1## wherein X is alkyl, --(CH.sub.2).sub.m --COOH or --O--(CH.sub.2).sub.n --COOH wherein m is 0-4 and n is 1-4; R is optionally substituted p-nitrophenyl; an optionally substituted thiophene residue; an optionally substituted thiazole residue; an optionally substituted benzothiazole residue; an optionally substituted benzoisothiazole residue; an N-alkylthiazole residue; or an optionally substituted 1,3,5-thiodiazole residue; as well as the alkali metal, alkaline earth metal and ammonium salts thereof.
摘要翻译：本发明提供γ-谷氨酰基-4-偶氮苯胺，其制备方法及其用作γ-谷氨酰转移酶测定的底物，其中X是烷基， - （CH 2）m -COOH或 -O-（CH 2）n -COOH，其中m为0-4，n为1-4; R是任选取代的对硝基苯基; 任选取代的噻吩残基; 任选取代的噻唑残基; 任选取代的苯并噻唑残基; 任选取代的苯并异噻唑残基; N-烷基噻唑残基; 或任选取代的1,3,5-噻二唑残基; 以及碱金属，碱土金属和铵盐。

9. 发明授权

US5068182A Substrates and reagents useful in determining .alpha.-amylase and a method for determining .alpha.-amylase 失效
标题翻译：用于测定（ALPHA） - 亚胺酶的基质和试剂和确定（ALPHA） - 亚胺酶的方法
公开(公告)号：US5068182A
公开(公告)日：1991-11-26
申请号：US288471
申请日：1988-12-22
申请人： Axel Schmidt , Herbert Von Der Eltz , Elli Rauscher
发明人： Axel Schmidt , Herbert Von Der Eltz , Elli Rauscher
IPC分类号： C12Q1/40 , C07H15/203 , C07H17/00
CPC分类号： C07H15/203 , C07H17/00
摘要： The present invention provides compounds of the general formula ##STR1## wherein R.sub.1 is a hydrogen atom, a methyl, ethyl, propyl, isopropyl, butyl, tert. butyl or 1-alkoxyalkyl radical or an optionally hydrophilically substituted cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, phenyl, tetrahydropyranyl, piperidinyl, N-methyl substituted piperidinyl or N-ethyl substituted piperidinyl, pyridinyl, thiophenyl or 1,1-dioxo-tetra- hydrothiopyranyl radical or an amino group which is optionally substituted by the same or different substituents selected from methyl, ethyl, propyl and isopropyl radicals. R.sub.2 is an oligoglucoside residue with 2, 3 or 4 glucose units and X is a hydrogen atom or an optically determinable residue. The present invention also provides processes for the preparation of compounds (I) and reagents for the determination of .alpha.-amylase containing them. Furthermore, the present invention provides a process for the determination of .alpha.-amylase using a compound of general formula (I).
摘要翻译：本发明提供通式为（I）的化合物，其中R 1为氢原子，甲基，乙基，丙基，异丙基，丁基，叔丁基。丁基或1-烷氧基烷基或任选亲水取代的环丙基，环丁基，环戊基，环己基，苯基，四氢吡喃基，哌啶基，N-甲基取代的哌啶基或N-乙基取代的哌啶基，吡啶基，噻吩基或1,1-二氧代 - 四氢硫代吡喃基基团或任选被相应或不同的选自甲基，乙基，丙基和异丙基的取代基取代的氨基。 R2是具有2,3或4个葡萄糖单元的低聚葡萄糖苷残基，X是氢原子或光学上可确定的残基。本发明还提供了制备用于测定含有它们的α-淀粉酶的化合物（I）和试剂的方法。此外，本发明提供使用通式（I）的化合物测定α-淀粉酶的方法。

10. 发明授权

US5473058A Process and reagent for the specific determination of pancreatic .alpha.-amylase 失效
标题翻译：用于胰腺α-淀粉酶特异性测定的方法和试剂
公开(公告)号：US5473058A
公开(公告)日：1995-12-05
申请号：US109678
申请日：1993-08-20
申请人： Axel Schmidt , Elli Rauscher , Herbert von der Eltz
发明人： Axel Schmidt , Elli Rauscher , Herbert von der Eltz
IPC分类号： C07H15/203 , C07H17/00 , C12Q1/40 , G01N33/573 , G01N33/577 , C07H15/00
CPC分类号： C07H15/203 , C07H17/00 , C12Q1/40 , G01N33/573 , C12Q2334/00 , Y10S435/962 , Y10S436/824
摘要： The present invention provides a process for the specific determination of pancreatic .alpha.-amylase in the presence of salivary .alpha.-amylase in body fluids by reaction with a system for the detection of .alpha.-amylase with the use of an inhibitor for salivary .alpha.-amylase, wherein, as substrate, there is used a compound of the general formula:-- ##STR1## in which R.sub.1 is a straight-chained or branched alkyl or alkoyl radical containing up to 6 carbon atoms, a cycloalkyl or cycloalkoxyl radical containing 3 to 6 carbon atoms or a benzoyl, benzyl or phenyl radical which is optionally hydrophilically substituted, R.sub.2 is a hydrogen atom or in which R.sub.1 and R.sub.2 together form a methylene bridge, the hydrogen atoms of which, independently of one another, can each be substituted by an alkyl radical containing up to 5 carbon atoms or a phenyl radical, n is 1, 2 or 3 and X is an optically determinable residue. The present invention also provides a reagent for the specific determination of pancreatic .alpha.-amylase, as well as new compounds for use in the above process and reagent.
摘要翻译：本发明提供了一种通过与用于唾液α-淀粉酶抑制剂的α-淀粉酶检测系统的反应来在体液中存在唾液中的α-淀粉酶来特异性测定胰腺α-淀粉酶的方法，其中，作为底物，使用以下通式的化合物：其中R 1是含有至多6个碳原子的直链或支链烷基或烷酰基，含有3个或更多个碳原子的环烷基或环烷氧基至6个碳原子或任选被亲水取代的苯甲酰基，苄基或苯基，R 2是氢原子或其中R 1和R 2一起形成亚甲基桥，其氢原子彼此独立地可以被取代通过含有至多5个碳原子的烷基或苯基，n是1,2或3，X是光学上可确定的残基。本发明还提供了用于胰腺α-淀粉酶的特异性测定的试剂，以及用于上述方法和试剂的新化合物。

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