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1. 发明申请

WO2009077087A1 PROCESS FOR THE PRODUCTION OF ALGAL BIOMASS WITH A HIGH LIPID CONTENT 审中-公开
标题翻译：用于生产具有高脂含量的藻类生物量的方法
公开(公告)号：WO2009077087A1
公开(公告)日：2009-06-25
申请号：PCT/EP2008/010291
申请日：2008-12-03
申请人： ENI S.P.A. , RISPOLI, Giacomo , FIORAVANTI, Emiliano , BIGNAZZI, Renzo , D'ADDARIO, Ezio, Nicola , DE FERRA, Francesca , CAPUANO, Federico
发明人： RISPOLI, Giacomo , FIORAVANTI, Emiliano , BIGNAZZI, Renzo , D'ADDARIO, Ezio, Nicola , DE FERRA, Francesca , CAPUANO, Federico
IPC分类号： C12N1/12
CPC分类号： C12N1/12 , C12M21/02 , C12M23/18 , C12M23/58 , C12M29/02 , C12M43/04 , C12M47/02 , C12M47/06
摘要： The invention relates to a process for the production of algal biomass with a high lipid content, comprising: (a) the production of inocula in order to effect phase (b), in photoreactors; (b) the massive cultivation of the algal biomass in open ponds, inoculated with phase (a); (c) a thickening phase of the algal biomass, effected blandly; (d) an induction phase of the lipid production, wherein modules are used consisting of photoreactors or open ponds; (e) a separation phase of the biomass with a high lipid content.
摘要翻译：本发明涉及一种生产具有高脂质含量的藻类生物质的方法，包括：（a）在光反应器中生产接种物以实现阶段（b）; （b）接种阶段（a）的露天池塘中大量培养藻类生物量; （c）藻类生物质的增稠期，平缓地进行; （d）脂质生产的诱导期，其中使用由光反应器或露天池组成的模块; （e）具有高脂质含量的生物质的分离阶段。

2. 发明申请

WO2009000534A1 PROCESS FOR THE CULTIVATION OF MICRO-ALGAE 审中-公开
标题翻译：微藻的培养方法
公开(公告)号：WO2009000534A1
公开(公告)日：2008-12-31
申请号：PCT/EP2008/005206
申请日：2008-06-17
申请人： ENI S.P.A. , RISPOLI, Giacomo , FIORAVANTI, Emiliano , CAPUANO, Federico , D'ADDARIO, Ezio, Nicola
发明人： RISPOLI, Giacomo , FIORAVANTI, Emiliano , CAPUANO, Federico , D'ADDARIO, Ezio, Nicola
IPC分类号： C12N1/12
CPC分类号： C12N1/12 , Y02W10/37
摘要： Process for the cultivation of micro-algae comprising the planting of an algal strain in an aqueous environment and the growth process, under solar irradiation, by continuously feeding a gaseous stream essentially consisting of carbon dioxide and a stream of nutrients based on nitrogen, consisting of wastewater from industrial plants, coming from secondary treatment processes.
摘要翻译：培养微藻的方法，包括在水溶液环境中种植藻类菌株，在太阳辐射下，通过连续供给基本上由二氧化碳组成的气流和基于氮的营养物流组成的藻类，由来自工业厂房的废水来自二次处理工艺。

3. 发明申请

WO2011070432A1 PROCESS FOR THE PRODUCTION OF BIO-OIL FROM PHOTOTROPHIC AND HETEROTROPHIC ALGAE 审中-公开
标题翻译：生物油生产过程从光热和异位酸生产
公开(公告)号：WO2011070432A1
公开(公告)日：2011-06-16
申请号：PCT/IB2010/003179
申请日：2010-12-07
申请人： ENI S.P.A. , D'ADDARIO, Ezio, Nicola , DE FERRA, Francesca , CAPUANO, Federico , MIGLIO, Roberta , BOSETTI, Aldo , CARNELLI, Lino
发明人： D'ADDARIO, Ezio, Nicola , DE FERRA, Francesca , CAPUANO, Federico , MIGLIO, Roberta , BOSETTI, Aldo , CARNELLI, Lino
IPC分类号： C12P7/64
CPC分类号： C12P7/649 , Y02E50/13
摘要： A process for the production of bio -oil from phototrophic and heterotrophic algae comprising: cultivating at least one phototrophic alga, under photoautotrophic conditions, in order to obtain a first aqueous suspension of algal biomass; - cultivating at least one heterotrophic alga, under heterotrophic conditions, in order to obtain a second aqueous suspension of algal biomass; - subjecting said first aqueous suspension of algal biomass to concentration in order to obtain a first concentrated aqueous suspension of algal biomass; - combining said first concentrated aqueous suspension of algal biomass with said second aqueous suspension of algal biomass obtaining a third aqueous suspension of algal biomass; - subjecting said third aqueous suspension of algal biomass to concentration in order to obtain a second concentrated aqueous suspension of algal biomass; - subjecting said second concentrated aqueous suspension of algal biomass to thermal treatment in order to obtain an oily phase comprising bio-oil and an aqueous phase comprising organic compounds.
摘要翻译：一种从光养和异养藻类生产生物油的方法，包括：在光自养条件下培养至少一种光营养藻，以获得藻类生物质的第一含水悬浮液; - 在异养条件下培养至少一种异养藻，以获得藻类生物质的第二水性悬浮液; - 对藻类生物质的所述第一水性悬浮液进行浓缩，以获得藻类生物质的第一浓缩水悬浮液; 将藻类生物质的所述第一浓缩水性悬浮液与藻类生物质的所述第二水性悬浮液相组合，得到藻类生物质的第三水性悬浮液; - 使藻类生物质的所述第三水性悬浮液浓缩，以获得藻类生物质的第二浓缩水悬浮液; - 将藻类生物质的所述第二浓缩水悬浮液经受热处理，以获得包含生物油和含有机化合物的水相的油相。

4. 发明申请

WO2010000416A1 PROCESS FOR THE EXTRACTION OF FATTY ACIDS FROM ALGAL BIOMASS 审中-公开
标题翻译：从ALGAL BIOMASS提取脂肪酸的方法
公开(公告)号：WO2010000416A1
公开(公告)日：2010-01-07
申请号：PCT/EP2009/004617
申请日：2009-06-19
申请人： ENI S.P.A. , D'ADDARIO, Ezio, Nicola , CAPUANO, Federico , D'ANGELI, Edoardo , MEDICI, Roberto
发明人： D'ADDARIO, Ezio, Nicola , CAPUANO, Federico , D'ANGELI, Edoardo , MEDICI, Roberto
IPC分类号： C11B1/10 , C11C1/04 , C12M1/107 , C12M1/00 , C12P7/64 , C11C3/00
CPC分类号： C11B1/10 , C11C1/04 , C11C3/003 , C12P7/6409 , Y02E50/13 , Y02E50/343
摘要： Process for the extraction of fatty acids from algal biomass comprising: producing an aqueous suspension of algal biomass; subjecting the aqueous suspension of algal biomass to acid hydrolysis and extraction by the addition of at least one non-polar organic solvent and at least one inorganic acid to said aqueous suspension of algal biomass, so as to obtain the following three phases: (i) a semisolid phase comprising a slurry of the algal biomass; (ii) an aqueous phase comprising inorganic compounds and hydrophilic organic compounds; (iii) an organic phase comprising fatty acids and hydrophobic organic compounds other than said fatty acids.
摘要翻译：从藻类生物质中提取脂肪酸的方法，包括：产生藻类生物质的水悬浮液; 通过向所述藻类生物质的水悬浮液中加入至少一种非极性有机溶剂和至少一种无机酸使藻类生物质的水悬浮液进行酸水解和提取，以获得以下三个阶段：（i）包含藻类生物质的浆料的半固体相; （ii）包含无机化合物和亲水性有机化合物的水相; （iii）包含除所述脂肪酸以外的脂肪酸和疏水性有机化合物的有机相。

5. 发明申请

WO2006066973A2 METHOD FOR THE PREPARATION OF A NICHEL CERMET FOR SOLID OXIDE FUEL CELLS 审中-公开
标题翻译：用于制备固体氧化物燃料电池的镍电池的方法
公开(公告)号：WO2006066973A2
公开(公告)日：2006-06-29
申请号：PCT/EP2005/014180
申请日：2005-12-23
申请人： ENI S.P.A. , ENITECNOLOGIE S.P.A. , PITTALIS, Francesco , CAPUANO, Federico , SABATINO, Luigina, Maria, Flora
发明人： PITTALIS, Francesco , CAPUANO, Federico , SABATINO, Luigina, Maria, Flora
CPC分类号： H01M4/8885 , C22C1/10 , C22C2001/1089 , H01M4/8621 , H01M4/9066 , H01M2004/8684 , H01M2008/1293
摘要： A method for the preparation of a ceramic-metallic composition (cermet) consisting of at least 70% by weight of a solid material comprising from 35 to 70% by weight of a metallic nickel phase and from 65 to 30% by weight of at least one oxide selected from oxides of transition metals and lanthanides, comprising the following steps in succession: i) preparing a mixture of powders comprising metallic nickel and at least one oxide different from nickel oxide, selected from oxides of transition metals and lanthanides; ii) laying said mixture of powders on a suitable surface, in the form of a layer having a thickness ranging from 2 to 1500 µm; iii) heating said layer under oxidative conditions until obtaining the desired sintering degree; and, optionally, iv) reducing the nickel oxide in the sintered product to metallic Ni.
摘要翻译：一种制备陶瓷 - 金属组合物（金属陶瓷）的方法，该陶瓷 - 金属组合物由至少70重量％的固体材料组成，所述固体材料包含35-70重量％的金属镍相和至少65重量％至30重量％一种选自过渡金属和镧系元素的氧化物的氧化物，包括以下步骤：i）制备包含金属镍和至少一种不同于氧化镍的氧化物的粉末的混合物，所述氧化镍选自过渡金属和镧系元素的氧化物; ii）将粉末混合物铺在合适的表面上，厚度为2至1500μm的层的形式; iii）在氧化条件下加热所述层直到获得所需烧结度; 和任选地，iv）将烧结产品中的氧化镍还原为金属Ni。

6. 发明申请

WO2015075630A1 PROCESS FOR THE EXTRACTION OF LIPIDS AND SUGARS FROM ALGAL BIOMASS 审中-公开
标题翻译：从ALGAL BIOMASS提取脂肪和糖的方法
公开(公告)号：WO2015075630A1
公开(公告)日：2015-05-28
申请号：PCT/IB2014/066130
申请日：2014-11-18
申请人： ENI S.P.A.
发明人： MASSETTI, Felicia , CAPUANO, Federico , MEDICI, Roberto , MIGLIO, Roberta
IPC分类号： C11C1/10 , C12N1/12 , C12P19/00
CPC分类号： C11B1/10 , C12N1/02 , C12N1/12 , C13K1/00 , C13K13/002 , C13K13/007 , Y02E50/13
摘要： Process for the extraction of lipids and sugars from algal biomass, which comprises: - producing an aqueous suspension of algal biomass; - bringing the pH of said aqueous suspension of algal biomass to a value higher than or equal to 10, preferably ranging from 10.2 to 12, obtaining an aqueous suspension of algal biomass at basic pH; - adding at least one anionic flocculant to said aqueous suspension of algal biomass at basic pH obtaining a concentrated algal biomass; - recovering said concentrated algal biomass; -subjecting said concentrated algal biomass to extraction of the lipids obtaining: (i) an organic phase comprising lipids; (ii) a semi-solid phase comprising a residue of said algal biomass; - subjecting said semi-solid phase (ii) to hydrolysis obtaining sugars. The lipids thus obtained can be advantageously used in the production of biodiesel or green diesel which can be used as such, or in a mixture with other fuels for motor vehicles. The sugars thus obtained can be advantageously used as carbon sources in fermentation processes for the production of lipids and also for the production of alcohols (e.g., ethanol, butanol). Said alcohols can be advantageously used as biofuels for motor vehicles or as components that can be added to fuels for motor vehicles.
摘要翻译：从藻类生物质中提取脂质和糖的方法，其包括： - 产生藻类生物质的水悬浮液; - 将藻类生物质的所述水性悬浮液的pH值提高到高于或等于10，优选为10.2至12的值，得到碱性pH下的藻类生物质的水悬浮液; - 在碱性pH下向藻类生物质的所述水性悬浮液中加入至少一种阴离子絮凝剂，得到浓缩藻类生物质; - 回收所述浓缩藻类生物质; - 将所述浓缩的藻类生物质引入所述脂质的提取中，获得：（i）包含脂质的有机相; （ii）包含所述藻类生物质的残余物的半固相; - 对所述半固相（ii）进行水解获得糖。由此获得的脂质可以有利地用于生产生物柴油或绿色柴油，其可以原样使用，或与用于机动车辆的其它燃料的混合物。由此获得的糖可有利地用作用于生产脂质的发酵方法中的碳源，也可用于生产醇（例如乙醇，丁醇）。所述醇可以有利地用作机动车辆的生物燃料或作为机动车辆燃料的组分。

7. 发明授权

EP3071680B1 PROCESS FOR THE EXTRACTION OF LIPIDS AND SUGARS FROM ALGAL BIOMASS 有权
标题翻译：从藻类生物质中提取脂质和糖的方法
公开(公告)号：EP3071680B1
公开(公告)日：2018-03-28
申请号：EP14828280.9
申请日：2014-11-18
申请人： ENI S.p.A.
发明人： MASSETTI, Felicia , CAPUANO, Federico , MEDICI, Roberto , MIGLIO, Roberta
IPC分类号： C11B1/10
CPC分类号： C11B1/10 , C12N1/02 , C12N1/12 , C13K1/00 , C13K13/002 , C13K13/007 , Y02E50/13
摘要： Process for the extraction of lipids and sugars from algal biomass, which comprises: —producing an aqueous suspension of algal biomass; —bringing the pH of said aqueous suspension of algal biomass to a value higher than or equal to 10, preferably ranging from 10.2 to 12, obtaining an aqueous suspension of algal biomass at basic pH; —adding at least one anionic flocculant to said aqueous suspension of algal biomass at basic pH obtaining a concentrated algal biomass; —recovering said concentrated algal biomass; —subjecting said concentrated algal biomass to extraction of the lipids obtaining: (i) an organic phase comprising lipids; (ii) a semi-solid phase comprising a residue of said algal biomass; —subjecting said semi-solid phase (ii) to hydrolysis obtaining sugars. The lipids thus obtained can be advantageously used in the production of biodiesel or green diesel which can be used as such, or in a mixture with other fuels for motor vehicles. The sugars thus obtained can be advantageously used as carbon sources in fermentation processes for the production of lipids and also for the production of alcohols (e.g., ethanol, butanol). Said alcohols can be advantageously used as biofuels for motor vehicles or as components that can be added to fuels for motor vehicles.

8. 发明公开

EP3071680A1 PROCESS FOR THE EXTRACTION OF LIPIDS AND SUGARS FROM ALGAL BIOMASS 有权
标题翻译：过程脂质的藻类生物质中提取和糖
公开(公告)号：EP3071680A1
公开(公告)日：2016-09-28
申请号：EP14828280.9
申请日：2014-11-18
申请人： ENI S.p.A.
发明人： MASSETTI, Felicia , CAPUANO, Federico , MEDICI, Roberto , MIGLIO, Roberta
IPC分类号： C11C1/10 , C12N1/12 , C12P19/00
CPC分类号： C11B1/10 , C12N1/02 , C12N1/12 , C13K1/00 , C13K13/002 , C13K13/007 , Y02E50/13
摘要： Process for the extraction of lipids and sugars from algal biomass, which comprises: —producing an aqueous suspension of algal biomass; —bringing the pH of said aqueous suspension of algal biomass to a value higher than or equal to 10, preferably ranging from 10.2 to 12, obtaining an aqueous suspension of algal biomass at basic pH; —adding at least one anionic flocculant to said aqueous suspension of algal biomass at basic pH obtaining a concentrated algal biomass; —recovering said concentrated algal biomass; —subjecting said concentrated algal biomass to extraction of the lipids obtaining: (i) an organic phase comprising lipids; (ii) a semi-solid phase comprising a residue of said algal biomass; —subjecting said semi-solid phase (ii) to hydrolysis obtaining sugars. The lipids thus obtained can be advantageously used in the production of biodiesel or green diesel which can be used as such, or in a mixture with other fuels for motor vehicles. The sugars thus obtained can be advantageously used as carbon sources in fermentation processes for the production of lipids and also for the production of alcohols (e.g., ethanol, butanol). Said alcohols can be advantageously used as biofuels for motor vehicles or as components that can be added to fuels for motor vehicles.

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