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1. 发明申请

US20130306481A1 THREE DIMENSIONAL MICROELECTRODE SYSTEM FOR DIELECTROPHORESIS 有权
标题翻译：三维微电子系统用于电介质
公开(公告)号：US20130306481A1
公开(公告)日：2013-11-21
申请号：US13945768
申请日：2013-07-18
申请人： Dietrich A. Dehlinger , Klint A. Rose , Maxim Shusteff , Christopher G. Bailey , Raymond P. Mariella, JR.
发明人： Dietrich A. Dehlinger , Klint A. Rose , Maxim Shusteff , Christopher G. Bailey , Raymond P. Mariella, JR.
IPC分类号： B03C5/00
CPC分类号： B03C5/005 , B01L2400/0424 , B03C5/026 , G01N27/447
摘要： A dielectrophoresis apparatus for separating particles from a sample, including an apparatus body; a dielectrophoresis channel in the apparatus body, the dielectrophoresis channel having a central axis, a bottom, a top, a first side, and a second side; a first mesa projecting into the dielectrophoresis channel from the bottom and extending from the first side across the dielectrophoresis channel to the second side, the first mesa extending at an angle to the central axis of the dielectrophoresis channel; a first electrode extending along the first mesa; a second mesa projecting into the dielectrophoresis channel from the bottom and extending from the first side across the dielectrophoresis channel to the second side, the second mesa extending at an angle to the central axis of the dielectrophoresis channel; a space between at least one of the first electrode and the second side or the second electrode and the second side; and a gap between the first electrode and the second electrode.
摘要翻译：一种用于从样品中分离颗粒的介电电泳装置，包括装置体; 所述设备主体中的介电电泳通道，所述介电电泳通道具有中心轴线，底部，顶部，第一侧面和第二侧面; 第一台面从底部突出到介电电泳通道中并且从第一侧穿过介电电泳通道延伸到第二侧，第一台面以与介电电泳通道的中心轴线成一定角度延伸; 沿第一台面延伸的第一电极; 从底部突出到介电电泳通道的第二台面，并且从第一侧穿过介电电泳通道延伸到第二侧，第二台面以与介电电泳通道的中心轴线成一定角度延伸; 第一电极和第二电极和第二电极和第二侧中的至少一个之间的空间; 以及第一电极和第二电极之间的间隙。

2. 发明申请

US20110259745A1 Three Dimensional Microelectrode System For Dielectrophoresis 有权
标题翻译：用于电泳的三维微电极系统
公开(公告)号：US20110259745A1
公开(公告)日：2011-10-27
申请号：US12843269
申请日：2010-07-26
申请人： Dietrich A. Dehlinger , Klint A. Rose , Maxim Shusteff , Christopher G. Bailey , Raymond P. Mariella, JR.
发明人： Dietrich A. Dehlinger , Klint A. Rose , Maxim Shusteff , Christopher G. Bailey , Raymond P. Mariella, JR.
IPC分类号： B03C5/02
CPC分类号： B03C5/005 , B01L2400/0424 , B03C5/026 , G01N27/447
摘要： A dielectrophoresis apparatus for separating particles from a sample, including an apparatus body; a dielectrophoresis channel in the apparatus body, the dielectrophoresis channel having a central axis, a bottom, a top, a first side, and a second side; a first mesa projecting into the dielectrophoresis channel from the bottom and extending from the first side across the dielectrophoresis channel to the second side, the first mesa extending at an angle to the central axis of the dielectrophoresis channel; a first electrode extending along the first mesa; a second mesa projecting into the dielectrophoresis channel from the bottom and extending from the first side across the dielectrophoresis channel to the second side, the second mesa extending at an angle to the central axis of the dielectrophoresis channel; a space between at least one of the first electrode and the second side or the second electrode and the second side; and a gap between the first electrode and the second electrode.
摘要翻译：一种用于从样品中分离颗粒的介电电泳装置，包括装置体; 所述设备主体中的介电电泳通道，所述介电电泳通道具有中心轴线，底部，顶部，第一侧面和第二侧面; 第一台面从底部突出到介电电泳通道中并且从第一侧穿过介电电泳通道延伸到第二侧，第一台面以与介电电泳通道的中心轴线成一定角度延伸; 沿第一台面延伸的第一电极; 第二台面从底部突出到介电电泳通道中，并从第一侧穿过介电电泳通道延伸到第二侧，第二台面以与介电电泳通道的中心轴成一定角度延伸; 第一电极和第二电极和第二电极和第二侧中的至少一个之间的空间; 以及第一电极和第二电极之间的间隙。

3. 发明授权

US08524064B2 Three dimensional microelectrode system for dielectrophoresis 有权
标题翻译：用于介电电泳的三维微电极系统
公开(公告)号：US08524064B2
公开(公告)日：2013-09-03
申请号：US12843269
申请日：2010-07-26
申请人： Dietrich A. Dehlinger , Klint A. Rose , Maxim Shusteff , Christopher G. Bailey , Raymond P. Mariella, Jr.
发明人： Dietrich A. Dehlinger , Klint A. Rose , Maxim Shusteff , Christopher G. Bailey , Raymond P. Mariella, Jr.
IPC分类号： G01N27/26 , G01N27/453 , B03C5/02 , B01D57/02
CPC分类号： B03C5/005 , B01L2400/0424 , B03C5/026 , G01N27/447
摘要： A dielectrophoresis apparatus for separating particles from a sample, including an apparatus body; a dielectrophoresis channel in the apparatus body, the dielectrophoresis channel having a central axis, a bottom, a top, a first side, and a second side; a first mesa projecting into the dielectrophoresis channel from the bottom and extending from the first side across the dielectrophoresis channel to the second side, the first mesa extending at an angle to the central axis of the dielectrophoresis channel; a first electrode extending along the first mesa; a second mesa projecting into the dielectrophoresis channel from the bottom and extending from the first side across the dielectrophoresis channel to the second side, the second mesa extending at an angle to the central axis of the dielectrophoresis channel; a space between at least one of the first electrode and the second side or the second electrode and the second side; and a gap between the first electrode and the second electrode.
摘要翻译：一种用于从样品中分离颗粒的介电电泳装置，包括装置体; 所述设备主体中的介电电泳通道，所述介电电泳通道具有中心轴线，底部，顶部，第一侧面和第二侧面; 第一台面从底部突出到介电电泳通道中并且从第一侧穿过介电电泳通道延伸到第二侧，第一台面以与介电电泳通道的中心轴线成一定角度延伸; 沿第一台面延伸的第一电极; 第二台面从底部突出到介电电泳通道中，并从第一侧穿过介电电泳通道延伸到第二侧，第二台面以与介电电泳通道的中心轴成一定角度延伸; 第一电极和第二电极和第二电极和第二侧中的至少一个之间的空间; 以及第一电极和第二电极之间的间隙。

4. 发明授权

US08727129B2 Microfluidic ultrasonic particle separators with engineered node locations and geometries 有权
标题翻译：具有工程节点位置和几何形状的微流体超声波颗粒分离器
公开(公告)号：US08727129B2
公开(公告)日：2014-05-20
申请号：US13571640
申请日：2012-08-10
申请人： Klint A. Rose , Karl A. Fisher , Douglas A. Wajda , Raymond P. Mariella, Jr. , Christopher Bailey , Dietrich Dehlinger , Maxim Shusteff , Byoungsok Jung , Kevin D. Ness
发明人： Klint A. Rose , Karl A. Fisher , Douglas A. Wajda , Raymond P. Mariella, Jr. , Christopher Bailey , Dietrich Dehlinger , Maxim Shusteff , Byoungsok Jung , Kevin D. Ness
IPC分类号： B03B5/00
CPC分类号： B01L3/50273 , B01D21/28 , B01D21/283 , B01D2221/10 , B01L3/502761 , B01L2200/0631 , B01L2200/0636 , B01L2200/0652 , B01L2300/0816 , B01L2300/0864 , B01L2400/0439 , B01L2400/0487 , G01N1/4077 , G01N33/48728 , G01N33/491 , G01N2001/4094
摘要： An ultrasonic microfluidic system includes a separation channel for conveying a sample fluid containing small particles and large particles, flowing substantially parallel, adjacent to a recovery fluid, with which it is in contact. An acoustic transducer produces an ultrasound standing wave, that generates a pressure field having at least one node of minimum pressure amplitude. An acoustic extension structure is located proximate to said separation channel for positioning said acoustic node off center in said acoustic area and concentrating the large particles in said recovery fluid stream.
摘要翻译：超声波微流体系统包括用于输送包含小颗粒和大颗粒的样品流体的分离通道，所述样品流体基本上平行于与其接触的回收流体相邻流动。声换能器产生超声驻波，其产生具有至少一个最小压力振幅节点的压力场。声学延伸结构位于所述分离通道附近，用于将所述声音节点偏离所述声学区域的中心，并将大颗粒集中在所述回收流体流中。

5. 发明申请

US20130043170A1 Microfluidic ultrasonic particle separators with engineered node locations and geometries 有权
标题翻译：具有工程节点位置和几何形状的微流体超声波颗粒分离器
公开(公告)号：US20130043170A1
公开(公告)日：2013-02-21
申请号：US13571640
申请日：2012-08-10
申请人： Klint A. Rose , Karl A. Fisher , Douglas A. Wajda , Raymond P. Mariella, JR. , Christopher Bailey , Dietrich Dehlinger , Maxim Shusteff , Byoungsok Jung , Kevin D. Ness
发明人： Klint A. Rose , Karl A. Fisher , Douglas A. Wajda , Raymond P. Mariella, JR. , Christopher Bailey , Dietrich Dehlinger , Maxim Shusteff , Byoungsok Jung , Kevin D. Ness
IPC分类号： B03B5/60
CPC分类号： B01L3/50273 , B01D21/28 , B01D21/283 , B01D2221/10 , B01L3/502761 , B01L2200/0631 , B01L2200/0636 , B01L2200/0652 , B01L2300/0816 , B01L2300/0864 , B01L2400/0439 , B01L2400/0487 , G01N1/4077 , G01N33/48728 , G01N33/491 , G01N2001/4094
摘要： An ultrasonic microfluidic system includes a separation channel for conveying a sample fluid containing small particles and large particles, flowing substantially parallel, adjacent to a recovery fluid, with which it is in contact. An acoustic transducer produces an ultrasound standing wave, that generates a pressure field having at least one node of minimum pressure amplitude. An acoustic extension structure is located proximate to said separation channel for positioning said acoustic node off center in said acoustic area and concentrating the large particles in said recovery fluid stream.
摘要翻译：超声波微流体系统包括用于输送包含小颗粒和大颗粒的样品流体的分离通道，所述样品流体基本上平行于与其接触的回收流体相邻流动。声换能器产生超声驻波，其产生具有至少一个最小压力振幅节点的压力场。声学延伸结构位于所述分离通道附近，用于将所述声音节点偏离所述声学区域的中心，并将大颗粒集中在所述回收流体流中。

6. 发明授权

US09144799B2 Modular microfluidic system for biological sample preparation 有权
标题翻译：用于生物样品制备的模块化微流体系统
公开(公告)号：US09144799B2
公开(公告)日：2015-09-29
申请号：US12943677
申请日：2010-11-10
申请人： Klint A. Rose , Raymond P. Mariella, Jr. , Christopher G. Bailey , Kevin Dean Ness
发明人： Klint A. Rose , Raymond P. Mariella, Jr. , Christopher G. Bailey , Kevin Dean Ness
IPC分类号： C12M1/33 , C12M3/08 , B01L3/00 , B03C5/02 , G01N35/00
CPC分类号： B01L3/502761 , B01L3/502753 , B01L2200/027 , B01L2200/04 , B01L2300/0681 , B01L2400/0436 , B01L2400/0439 , B03C5/026 , B03C2201/26 , G01N2035/00326
摘要： A reconfigurable modular microfluidic system for preparation of a biological sample including a series of reconfigurable modules for automated sample preparation adapted to selectively include a) a microfluidic acoustic focusing filter module, b) a dielectrophoresis bacteria filter module, c) a dielectrophoresis virus filter module, d) an isotachophoresis nucleic acid filter module, e) a lyses module, and f) an isotachophoresis-based nucleic acid filter.
摘要翻译：一种用于制备生物样品的可重构模块化微流体系统，包括用于自动化样品制备的一系列可重新配置模块，其适于选择性地包括a）微流体声学聚焦滤光器模块，b）介电电泳细菌滤膜模块，c）介电泳病毒过滤器模块， d）等速电泳核酸过滤器模块，e）裂解模块和f）基于等电泳的核酸过滤器。

7. 发明申请

US20110124098A1 Modular Microfluidic System for Biological Sample Preparation 有权
标题翻译：用于生物样品制备的模块化微流体系统
公开(公告)号：US20110124098A1
公开(公告)日：2011-05-26
申请号：US12943677
申请日：2010-11-10
申请人： Klint A. Rose , Raymond P. Mariella, JR. , Christopher G. Bailey , Kevin Dean Ness
发明人： Klint A. Rose , Raymond P. Mariella, JR. , Christopher G. Bailey , Kevin Dean Ness
IPC分类号： C12M1/12
CPC分类号： B01L3/502761 , B01L3/502753 , B01L2200/027 , B01L2200/04 , B01L2300/0681 , B01L2400/0436 , B01L2400/0439 , B03C5/026 , B03C2201/26 , G01N2035/00326
摘要： A reconfigurable modular microfluidic system for preparation of a biological sample including a series of reconfigurable modules for automated sample preparation adapted to selectively include a) a microfluidic acoustic focusing filter module, b) a dielectrophoresis bacteria filter module, c) a dielectrophoresis virus filter module, d) an isotachophoresis nucleic acid filter module, e) a lyses module, and f) an isotachophoresis-based nucleic acid filter.
摘要翻译：一种用于制备生物样品的可重构模块化微流体系统，包括用于自动化样品制备的一系列可重新配置模块，其适于选择性地包括a）微流体声学聚焦滤光器模块，b）介电电泳细菌滤膜模块，c）介电泳病毒过滤器模块， d）等速电泳核酸过滤器模块，e）裂解模块和f）基于等电泳的核酸过滤器。

8. 发明授权

US08999129B2 Liquid and gel electrodes for transverse free flow electrophoresis 有权
标题翻译：用于横向自由电泳的液体和凝胶电极
公开(公告)号：US08999129B2
公开(公告)日：2015-04-07
申请号：US13413161
申请日：2012-03-06
申请人： Byoungsok Jung , Klint A. Rose , Maxim Shusteff , Alexandre Persat , Juan Santiago
发明人： Byoungsok Jung , Klint A. Rose , Maxim Shusteff , Alexandre Persat , Juan Santiago
IPC分类号： G01N27/447 , B01D57/02 , B01D43/00
CPC分类号： B01D43/00 , B01D57/02 , G01N27/44769
摘要： The present invention provides a mechanism for separating or isolating charged particles under the influence of an electric field without metal electrodes being in direct contact with the sample solution. The metal electrodes normally in contact with the sample are replaced with high conductivity fluid electrodes situated parallel and adjacent to the sample. When the fluid electrodes transmit the electric field across the sample, particles within the sample migrate according to their electrophoretic mobility.
摘要翻译：本发明提供了一种用于在没有金属电极与样品溶液直接接触的电场的影响下分离或分离带电粒子的机理。通常与样品接触的金属电极被替换为与样品平行且相邻的高导电性流体电极。当流体电极跨越样品传播电场时，样品内的颗粒根据其电泳迁移率而迁移。

9. 发明申请

US20120228141A1 LIQUID AND GEL ELECTRODES FOR TRANSVERSE FREE FLOW ELECTROPHORESIS 有权
标题翻译：液体和凝胶电极用于横向自由流动电泳
公开(公告)号：US20120228141A1
公开(公告)日：2012-09-13
申请号：US13413161
申请日：2012-03-06
申请人： Byoungsok Jung , Klint A. Rose , Maxim Shusteff , Alexandre Persat , Juan Santiago
发明人： Byoungsok Jung , Klint A. Rose , Maxim Shusteff , Alexandre Persat , Juan Santiago
IPC分类号： C25B9/00 , B01D43/00
CPC分类号： B01D43/00 , B01D57/02 , G01N27/44769
摘要： The present invention provides a mechanism for separating or isolating charged particles under the influence of an electric field without metal electrodes being in direct contact with the sample solution. The metal electrodes normally in contact with the sample are replaced with high conductivity fluid electrodes situated parallel and adjacent to the sample. When the fluid electrodes transmit the electric field across the sample, particles within the sample migrate according to their electrophoretic mobility.
摘要翻译：本发明提供了一种用于在没有金属电极与样品溶液直接接触的电场的影响下分离或分离带电粒子的机理。通常与样品接触的金属电极被替换为与样品平行且相邻的高导电性流体电极。当流体电极跨越样品传播电场时，样品内的颗粒根据其电泳迁移率而迁移。

10. 发明授权

US09290855B2 Stabilization of green bodies via sacrificial gelling agent during electrophoretic deposition 有权
标题翻译：在电泳沉积期间通过牺牲胶凝剂稳定生物体
公开(公告)号：US09290855B2
公开(公告)日：2016-03-22
申请号：US13453933
申请日：2012-04-23
申请人： Marcus A. Worsley , Joshua D. Kuntz , Klint A. Rose
发明人： Marcus A. Worsley , Joshua D. Kuntz , Klint A. Rose
IPC分类号： B32B7/02 , D02G3/00 , C04B35/51 , C25D13/02 , C25D5/10 , H01S3/16 , H01S3/06
CPC分类号： C25D13/02 , C25D5/10 , H01S3/0612 , H01S3/1685 , Y10T428/12021 , Y10T428/12458 , Y10T428/24926
摘要： In one embodiment, a method for electrophoretic deposition of a three-dimensionally patterned green body includes suspending a first material in a gelling agent above a patterned electrode of an electrophoretic deposition (EPD) chamber, and gelling the suspension while applying a first electric field to the suspension to cause desired patterning of the first material in a resulting gelation. In another embodiment, a ceramic, metal, or cermet includes a plurality of layers, wherein each layer includes a gradient in composition, microstructure, and/or density in an x-y plane oriented parallel to a plane of deposition of the plurality of layers along a predetermined distance in a z-direction perpendicular to the plane of deposition.
摘要翻译：在一个实施例中，三维图案化的生坯的电泳沉积方法包括将第一材料悬浮在电泳沉积（EPD）室的图案化电极之上的胶凝剂中，并在施加第一电场的同时胶凝悬浮液所述悬浮液在所得凝胶化中引起所述第一材料的所需图案化。在另一个实施方案中，陶瓷，金属或金属陶瓷包括多个层，其中每个层包括在平行于沿着多个层的沉积平面定向的xy平面中的组成，微观结构和/或密度的梯度在垂直于沉积平面的z方向上的预定距离。

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