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    • 2. 发明申请
    • ENZYMATIC OIL-DEGUMMING METHOD
    • ENIYYY油脂脱水方法
    • WO2006008508A1
    • 2006-01-26
    • PCT/GB2005/002823
    • 2005-07-18
    • DANISCO A/SSOE, Jorn, BorchTURNER, Mark
    • SOE, Jorn, BorchTURNER, Mark
    • C11B3/00
    • C12N9/1029A21D8/042A23J7/00C11B3/003C12N9/20C12P7/6418C12P7/6445C12P7/6481C12Y301/01003
    • A process of enzymatic degumming edible oils, comprising treating the edible oil with a lipid acyltransferase so as to transfer an acyl group from a major part of the phospholipid to one or more acyl acceptors, wherein the acyl acceptor may be any compound comprising a hydroxy group. In one embodiment preferably the acyl acceptor is water and in another embodiment preferably the acyl acceptor is one or more sterols and/or stanols. When the acyl acceptor is a stanol and/or sterol, one or more sterol esters and/or stanol esters are produced. The lipid acyltransferase for use in the process of the present invention may comprises one or more of the following amino acid sequences: SEQ ID No. 1, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 7, SEQ ID No. 8, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, or SEQ ID No. 15, SEQ ID No. 16, SEQ ID No. 17, SEQ ID No. 18, SEQ ID No. 36, SEQ ID No. 38, SEQ ID No. 40, SEQ ID No. 41, SEQ ID No 45, SEQ ID No. 47, SEQ ID No. 50 or an amino acid sequence which has 75% or more identity thereto. A novel lipid acyltransferase comprising the amino acid sequence shown as SEQ ID No. 16 is also taught.
    • 一种酶促脱脂食用油的方法,包括用脂质酰基转移酶处理食用油,以将酰基从大部分磷脂转移到一个或多个酰基受体上,其中酰基受体可以是包含羟基的任何化合物 。 在一个实施方案中,优选地,酰基受体是水,并且在另一个实施方案中,优选酰基受体是一种或多种甾醇和/或甾烷醇。 当酰基受体是甾烷醇和/或甾醇时,产生一种或多种甾醇酯和/或甾烷醇酯。 用于本发明方法的脂质酰基转移酶可以包含以下一个或多个氨基酸序列:SEQ ID No.1,SEQ ID No.3,SEQ ID No.4,SEQ ID No.5,SEQ ID No. 6号,SEQ ID No.7,SEQ ID No.8,SEQ ID No.9,SEQ ID No.10,SEQ ID No.11,SEQ ID No.12,SEQ ID No.13,SEQ ID No. 14,或SEQ ID No.15,SEQ ID No.16,SEQ ID No.17,SEQ ID No.18,SEQ ID No.36,SEQ ID No.38,SEQ ID No.40,SEQ ID No.41 ,SEQ ID No.45,SEQ ID No.47,SEQ ID No.50或与其同一性为75%以上的氨基酸序列。 还教导了包含SEQ ID No.16所示的氨基酸序列的新型脂质酰基转移酶。
    • 7. 发明授权
    • ENZYMATIC OIL-DEGUMMING METHOD
    • ENZYMATIC OIL-DEGUMMING法
    • EP1791933B1
    • 2011-06-29
    • EP05761577.5
    • 2005-07-18
    • DANISCO A/S
    • SOE, Jorn, BorchTURNER, Mark
    • C11B3/00C11C3/00C07F9/10
    • C12N9/1029A21D8/042A23J7/00C11B3/003C12N9/20C12P7/6418C12P7/6445C12P7/6481C12Y301/01003
    • A process of enzymatic degumming edible oils, comprising treating the edible oil with a lipid acyltransferase so as to transfer an acyl group from a major part of the phospholipid to one or more acyl acceptors, wherein the acyl acceptor may be any compound comprising a hydroxy group. In one embodiment preferably the acyl acceptor is water and in another embodiment preferably the acyl acceptor is one or more sterols and/or stanols. When the acyl acceptor is a stanol and/or sterol, one or more sterol esters and/or stanol esters are produced. The lipid acyltransferase for use in the process of the present invention may comprises one or more of the following amino acid sequences: SEQ ID No. 1, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 7, SEQ ID No. 8, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, or SEQ ID No. 15, SEQ ID No. 16, SEQ ID No. 17, SEQ ID No. 18, SEQ ID No. 36, SEQ ID No. 38, SEQ ID No. 40, SEQ ID No. 41, SEQ ID No 45, SEQ ID No. 47, SEQ ID No. 50 or an amino acid sequence which has 75% or more identity thereto. A novel lipid acyltransferase comprising the amino acid sequence shown as SEQ ID No. 16 is also taught.
    • 一种酶促脱胶食用油的方法,包括用脂质酰基转移酶处理食用油以便将酰基从大部分磷脂转移到一个或多个酰基受体,其中酰基受体可以是任何含有羟基的化合物 。 在一个实施方案中,优选酰基受体是水,在另一个实施方案中,优选酰基受体是一种或多种甾醇和/或甾烷醇。 当酰基受体是甾烷醇和/或甾醇时,产生一种或多种甾醇酯和/或甾烷醇酯。 用于本发明方法的脂质酰基转移酶可以包含一个或多个以下氨基酸序列:SEQ ID No.1,SEQ ID No.3,SEQ ID No.4,SEQ ID No.5,SEQ ID No.5 序列号6,序列号7,序列号8,序列号9,序列号10,序列号11,序列号12,序列号13,序列号 14或SEQ ID No.15,SEQ ID No.16,SEQ ID No.17,SEQ ID No.18,SEQ ID No.36,SEQ ID No.38,SEQ ID No.40,SEQ ID No.41 ,SEQ ID No 45,SEQ ID No.47,SEQ ID No.50或与其具有75%或更多同一性的氨基酸序列。 还教导了包含如SEQ ID No.16所示的氨基酸序列的新型脂质酰基转移酶。