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1. 发明申请

WO2023083976A1 RECOMBINANT MILK POLYPEPTIDE COMPOSITIONS FREE OF ASPARTYL PROTEASE ACTIVITY 审中-公开
公开(公告)号：WO2023083976A1
公开(公告)日：2023-05-19
申请号：PCT/EP2022/081513
申请日：2022-11-10
申请人： DSM IP ASSETS B.V.
发明人： VAN DER HOEVEN, Robertus Antonius Mijndert , ANEMA, Skelte-Gerald , MEIJRINK, Bernard , HEWITT, Sheelagh Ann , WELMAN, Alan David , HILL, Jeremy Paul
IPC分类号： A23C11/08 , A23C21/04 , A23J1/18 , C07K14/47 , C12N15/80 , C12N15/81
摘要： The present invention relates to food products comprising recombinant polypeptide compositions that are substantially free of aspartyl protease-like activity. The invention also relates to compositions comprising recombinant milk polypeptides and that are substantially free of aspartyl protease-like activity; and methods and tools for manufacture of such compositions.

2. 发明申请

WO2013110673A1 DITERPENE PRODUCTION 审中-公开
标题翻译：地面生产
公开(公告)号：WO2013110673A1
公开(公告)日：2013-08-01
申请号：PCT/EP2013/051262
申请日：2013-01-23
申请人： DSM IP ASSETS B.V.
发明人： LEHMANN, Martin , TRUEHEART, Joshua , ZWARTJENS, Priscilla , WU, Liang , BOER, Viktor , SAGT, Cornelis, Maria, Jacobus , KUMAR, Manoj , MEIJRINK, Bernard
IPC分类号： A23L1/236 , C12N9/02 , C12N9/88 , C12N9/90 , C12P5/00
CPC分类号： C12P19/56 , A23K20/10 , A23K20/121 , A23K20/163 , A23L2/60 , A23L27/36 , A23V2002/00 , C07H15/256 , C12N9/0073 , C12N9/1051 , C12N9/88 , C12N9/90 , C12N15/52 , C12P5/007 , C12Y114/13078 , C12Y204/01035 , C12Y402/03019 , C12Y505/01013
摘要： The present invention relates to a recombinant microorganism comprising one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol. The recombinant microorganism may also be capable of expressing one or more UDP-glucosyltransferases such that the microorganism is capable of producing one or more steviol glycosides.
摘要翻译：本发明涉及包含一个或多个核苷酸序列的重组微生物，所述核苷酸序列编码：具有入 - copalyl焦磷酸合成酶活性的多肽; 具有ent-Kaurene合酶活性的多肽; 具有ent-Kaurene氧化酶活性的多肽; 和具有山梨酸13-羟化酶活性的多肽，由此所述核苷酸序列的表达赋予所述微生物至少产生甜菊醇的能力。重组微生物还可能能够表达一种或多种UDP-葡糖基转移酶，使得微生物能够产生一种或多种甜菊糖苷。

3. 发明申请

WO2016110453A1 A CRISPR-CAS SYSTEM FOR A FILAMENTOUS FUNGAL HOST CELL 审中-公开
标题翻译：用于丝状真菌宿主细胞的CRISPR-CAS系统
公开(公告)号：WO2016110453A1
公开(公告)日：2016-07-14
申请号：PCT/EP2016/000029
申请日：2016-01-06
申请人： DSM IP ASSETS B.V. , RIJKSUNIVERSITEIT GRONINGEN
发明人： MEIJRINK, Bernard , VERWAAL, René , GIELESEN, Bianca Elisabeth Maria , ROUBOS, Johannes Andries , NYGÅRD, Yvonne Irene , BOVENBERG, Roelof Arij Lans , DRIESSEN, Arnold Jacob Mathieu , POHL, Carsten
IPC分类号： C12N15/90
CPC分类号： C12N15/902 , C12N15/102 , C12N15/111 , C12N15/905 , C12N2310/10 , C12N2310/20
摘要： The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a filamentous fungal host cell.
摘要翻译：本发明涉及分子生物学和细胞生物学领域。更具体地说，本发明涉及用于丝状真菌宿主细胞的CRISPR-CAS系统。

4. 发明申请

WO2015181311A1 PROCESS FOR PRODUCING ITACONIC ACID AND ITACONIC ACID ESTERS UNDER ANAEROBIC CONDITIONS 审中-公开
标题翻译：在厌氧条件下生产异柠檬酸和酸性酯的方法
公开(公告)号：WO2015181311A1
公开(公告)日：2015-12-03
申请号：PCT/EP2015/061883
申请日：2015-05-28
申请人： DSM IP ASSETS B.V.
发明人： ZHAO, Zheng , MEIJRINK, Bernard , HOEVEN, VAN DER, Robertus Antonius Mijndert , WU, Liang , ROUBOS, Johannes Andries , BROERS, Nicolette Jasmijn , HARTMANS, Sybe
IPC分类号： C12P7/46 , C12P7/62
CPC分类号： C12P7/62 , C12P7/46
摘要： The present invention relates to a method for the production of itaconic acid or an ester of itaconic acid, which method comprises fermenting a recombinant cell capable of producing itaconic acid or an ester of itaconic acid in a suitable fermentation medium under anaerobic conditions, wherein the itaconic acid or ester of itaconic acid is produced.
摘要翻译：本发明涉及一种生产衣康酸或衣康酸酯的方法，该方法包括在厌氧条件下，在合适的发酵培养基中发酵能够生产衣康酸或衣康酸酯的重组细胞，其中衣康酸产生酸或衣康酸的酯。

5. 发明申请

WO2016050917A1 METHODS FOR GENOMIC MODIFICATION 审中-公开
标题翻译：基因修饰方法
公开(公告)号：WO2016050917A1
公开(公告)日：2016-04-07
申请号：PCT/EP2015/072710
申请日：2015-10-01
申请人： DSM IP ASSETS B.V.
发明人： VOLLEBREGT, Adrianus, Wilhelmus, Hermanus , VONDERVOORT, VAN DE, Peter, Jozef, Ida , MEIJRINK, Bernard , ROUBOS, Johannes, Andries , TOUW-RIEL, Hesselien , BERG, VAN DEN, Marco, Alexander
IPC分类号： C12N15/63 , C12N15/65 , C12N15/80 , C12N15/90
CPC分类号： C12N15/80 , C12N9/22 , C12N15/102
摘要： A method for the modification of the genome of a fungal host cell at a target locus, the method comprises: (a) providing a fungal host cell comprising in its chromosome a target locus, the target locus comprising a recognition site for a nuclease; and (b) introducing into said cell the nuclease protein or mRNA encoding a nuclease protein which cleaves DNA at the recognition site such that recombination occurs at the target locus, thereby to modify the genome of the host cell at the target locus.
摘要翻译：一种用于在目标基因座处修饰真菌宿主细胞的基因组的方法，所述方法包括：（a）提供在其染色体中包含靶基因座的真菌宿主细胞，所述靶基因座包含核酸酶的识别位点; 和（b）向所述细胞中引入编码核酸酶蛋白的核酸酶蛋白或mRNA，所述核酸酶蛋白在识别位点切割DNA，使得在靶基因座处发生重组，从而修饰靶基因座上宿主细胞的基因组。

6. 发明申请

WO2015181310A2 ITACONIC ACID AND ITACONATE METHYLESTER AND DIMETHYLESTER PRODUCTION 审中-公开
标题翻译：异柠檬酸和甲基丙烯酸甲酯和二甲醚生产
公开(公告)号：WO2015181310A2
公开(公告)日：2015-12-03
申请号：PCT/EP2015/061882
申请日：2015-05-28
申请人： DSM IP ASSETS B.V.
发明人： ZHAO, Zheng , MEIJRINK, Bernard , HOEVEN, VAN DER, Robertus Antonius Mijndert , WU, Liang , ROUBOS, Johannes Andries
IPC分类号： C12N15/63
CPC分类号： C12P7/44 , C07K14/395 , C12N9/0008 , C12N9/1025 , C12N9/1029 , C12N9/1217 , C12N9/88 , C12N9/93 , C12N15/52 , C12P7/62 , C12Y203/03001 , C12Y401/01006 , C12Y401/01031 , C12Y401/01032 , C12Y604/01001
摘要： The present invention relates to a recombinant yeast cell which is capable of producing one or more of 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate. The invention also relates to a recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: - a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and - a nucleic acid encoding a polypeptide which catalyzes a reaction towards acetyl CoA. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate.
摘要翻译：本发明涉及能够产生衣康酸4-甲酯，衣康酸1-甲酯或衣康酸1,4-二甲酯中的一种或多种的重组酵母细胞。本发明还涉及能够产生衣康酸并且过表达的重组酵母细胞： - 编码具有顺 - 乌头酸脱羧酶活性的多肽的核酸; 以及 - 编码催化向乙酰辅酶A反应的多肽的核酸。这些重组酵母细胞可用于生产衣康酸，衣康酸4-甲酯，衣康酸1-甲酯或衣康酸1,4-二甲酯的方法。

7. 发明申请

WO2007147827A3 PRODUCTION OF PRAVASTATIN 审中-公开
公开(公告)号：WO2007147827A3
公开(公告)日：2007-12-27
申请号：PCT/EP2007/056096
申请日：2007-06-19
申请人： DSM IP Assets B.V. , BERG, VAN DEN, Marco Alexander , MEIJRINK, Bernard
发明人： BERG, VAN DEN, Marco Alexander , MEIJRINK, Bernard
IPC分类号： C12N15/80 , C12P7/42 , C12P7/62
摘要： The present invention provides a microorganism containing a compactin biosynth esis gene and a gene for conversion of compactin into pravastatin. In a pref erred example, said compactin biosynthesis gene is mlcA and/or mlcB and/or mlcC and/or mlcD and/or mlcE and/or mlcF and/or mlcG and/or mlcH and/or mlcR and said gene for conversion of compactin into pravastatin is a hydroxylase gene. Furthermore, the present invention provides a method for producing a compound of interest such as a statin. In a preferred example said statin is pravastatin.

8. 发明申请

WO2007122249A1 PRODUCTION OF COMPOUNDS IN A RECOMBINANT HOST 审中-公开
标题翻译：在重组生物中生产化合物
公开(公告)号：WO2007122249A1
公开(公告)日：2007-11-01
申请号：PCT/EP2007/054045
申请日：2007-04-25
申请人： DSM IP Assets B.V. , BOVENBERG, Roelof, Ary, Lans , VAN DEN BERG, Marco Alexander , HAGE, Suzanne , KLAASSEN, Paul , MEIJRINK, Bernard , RAAMSDONK, Lourina, Madeleine
发明人： BOVENBERG, Roelof, Ary, Lans , VAN DEN BERG, Marco Alexander , HAGE, Suzanne , KLAASSEN, Paul , MEIJRINK, Bernard , RAAMSDONK, Lourina, Madeleine
IPC分类号： C07K14/385 , C12N1/16
CPC分类号： C12N9/1029 , C12N9/0004 , C12N9/93 , C12P7/02 , C12P13/02 , C12P17/06 , C12P17/181 , C12P17/182 , C12P17/188
摘要： The present invention provides a recombinant Penicillium chrysogenum strain characterized in that a gene selected from the list consisting of penDE, pcbAB and pcbC is inactivated and the use of such a strain for the preparation of a compound of interest. Furthermore, it is an object of the present invention to provide a method for the production of a compound of interest in a eukaryotic recombinant microorganism comprising the steps of : (a) Reducing the level of secondary metabolite production in said microorganism with 50-100%; (b) Introducing a heterologous gene into said microorganism
摘要翻译：本发明提供重组产黄青霉菌株，其特征在于，从PenDE，pcbAB和pcbC列表中选出的基因被灭活，并且使用这样的菌株来制备感兴趣的化合物。此外，本发明的目的是提供一种在真核重组微生物中生产感兴趣的化合物的方法，包括以下步骤：（a）降低所述微生物中次级代谢产物的产生水平，其中50-100％ ; （b）将异源基因引入所述微生物

9. 发明申请

WO2016110511A1 A CRISPR-CAS SYSTEM FOR A LIPOLYTIC YEAST HOST CELL 审中-公开
标题翻译：一种用于脂质性YEAST HOST细胞的CRISPR-CAS系统
公开(公告)号：WO2016110511A1
公开(公告)日：2016-07-14
申请号：PCT/EP2016/050135
申请日：2016-01-06
申请人： DSM IP ASSETS B.V.
发明人： MEIJRINK, Bernard , VERWAAL, René , GIELESEN, Bianca Elisabeth Maria , ROUBOS, Johannes Andries
IPC分类号： C12N15/90 , C12N15/63
CPC分类号： C12N15/905 , C12N9/22 , C12N15/11 , C12N15/63 , C12N2310/20
摘要： The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.
摘要翻译：本发明涉及分子生物学和细胞生物学领域。更具体地，本发明涉及用于脂解酵母宿主细胞的CRISPR-CAS系统。

10. 发明申请

WO2015181312A2 CELLS FOR ITACONIC ACID PRODUCTION 审中-公开
标题翻译：用于生产酸的细胞
公开(公告)号：WO2015181312A2
公开(公告)日：2015-12-03
申请号：PCT/EP2015/061884
申请日：2015-05-28
申请人： DSM IP ASSETS B.V.
发明人： ZHAO, Zheng , MEIJRINK, Bernard , HOEVEN, VAN DER, Robertus Antonius Mijndert , WU, Liang , ROUBOS, Johannes Andries , GELDER, VAN, Marina
IPC分类号： C12N15/52 , C12P7/46 , C12P7/62
CPC分类号： C12P7/62 , C12N15/52 , C12P7/46
摘要： The present invention relates to a recombinant cell which is capable of producing one or more of itaconic acid,4-methyl itaconate or 1-methyl itaconate, wherein the said recombinant microorganism has been modified in its genome such that it results in a deficiency in the production of a trans-aconitate methyltransferase. A recombinant yeast cell which is capable of producing itaconic acid and which over expresses: -a nucleic 1 acid encoding a polypeptide having cis-aconitate decarboxylase activity; and-one or more nucleic acids encoding polypeptides which separately or together catalyze a reaction towards acetyl CoA,wherein the said recombinant microorganism has been modified in its genome such that it results in a deficiency in the production of a trans- aconitate methyltransferase. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate or 1-methyl itaconate.
摘要翻译：本发明涉及能够产生衣康酸，衣康酸4-甲酯或衣康酸1-甲酯中的一种或多种的重组细胞，其中所述重组微生物已经在其基因组中被修饰，从而导致其中的缺陷产生反式乌头碱甲基转移酶。能够产生衣康酸并且过表达的重组酵母细胞： - 编码具有顺 - 乌头酸脱羧酶活性的多肽的核酸1酸; 一个或多个编码多肽的核酸，其单独或一起催化向乙酰CoA的反应，其中所述重组微生物已经在其基因组中被修饰，从而导致转基因甲基转移酶的产生缺陷。这些重组酵母细胞可用于生产衣康酸，衣康酸4-甲酯或衣康酸1-甲酯的方法。

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