专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2006128590A1 HYDROLASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 审中-公开
标题翻译：水解物，核酸编码方法及其使用方法
公开(公告)号：WO2006128590A1
公开(公告)日：2006-12-07
申请号：PCT/EP2006/004736
申请日：2006-05-18
申请人： DSM IP ASSETS B.V. , MINK, Daniel , KIERKELS, Joannes, Gerardus, Theodorus , DESANTIS, Grace , FARWELL, Robert , WHIPPLE, Lawrence , BURK, Mark
发明人： MINK, Daniel , KIERKELS, Joannes, Gerardus, Theodorus , DESANTIS, Grace , FARWELL, Robert , WHIPPLE, Lawrence , BURK, Mark
IPC分类号： C12P41/00
CPC分类号： C12P7/62 , C12N9/16 , C12P17/06 , C12P41/005
摘要： The invention provides hydrolases, polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides, e.g., enzymes, having a hydrolase activity, e.g., an esterase, acylase, lipase, phospholipase (e.g., phospholipase A, B, C and/or D activity, patatin activity, lipid acyl hydrolase (LAH) activity) or protease activity, including thermostable and thermotolerant hydrolase activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The hydrolase activities of the polypeptides and peptides of the invention include esterase activity, lipase activity (hydrolysis of lipids), acidolysis reactions (to replace an esterified fatty acid with a free fatty acid), transesterification reactions (exchange of fatty acids between triglycerides), ester synthesis, ester interchange reactions, phospholipase activity and protease activity (hydrolysis of peptide bonds). The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts, including the manufacture of cosmetics and nutraceuticals. In another aspect, the polypeptides of the invention are used to synthesize enantiomerically pure chiral products.
摘要翻译：本发明提供水解酶，编码它们的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。一方面，本发明涉及具有水解酶活性的多肽，例如酯酶，酰基转移酶，脂肪酶，磷脂酶（例如，磷脂酶A，B，C和/或D活性，patatin活性，脂质酰基水解酶（LAH）活性）或蛋白酶活性，包括热稳定和耐热水解酶活性，以及编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和多肽。本发明的多肽和肽的水解酶活性包括酯酶活性，脂肪酶活性（脂质水解），酸解反应（用游离脂肪酸代替酯化脂肪酸），酯交换反应（甘油三酸酯之间的脂肪酸交换），酯合成，酯交换反应，磷脂酶活性和蛋白酶活性（肽键的水解）。本发明的多肽可用于各种药物，农业和工业环境中，包括制造化妆品和营养品。另一方面，本发明的多肽用于合成对映体纯的手性产物。

2. 发明申请

WO2003097810A3 NITRILASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 审中-公开
公开(公告)号：WO2003097810A3
公开(公告)日：2003-11-27
申请号：PCT/US2003/015712
申请日：2003-05-15
申请人： DIVERSA CORPORATION , DESANTIS, Grace , SHORT, Jay, M. , BURK, Mark , WONG, Kevin , FARWELL, Robert , CHATMAN, Kelly
发明人： DESANTIS, Grace , SHORT, Jay, M. , BURK, Mark , WONG, Kevin , FARWELL, Robert , CHATMAN, Kelly
IPC分类号： C12N9/00
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.

3. 发明申请

WO2003106415A3 PROCESSES FOR MAKING (R)-ETHYL 4-CYANO-3-HYDROXYBUTYRIC ACID 审中-公开
公开(公告)号：WO2003106415A3
公开(公告)日：2003-12-24
申请号：PCT/US2003/018840
申请日：2003-06-13
申请人： DIVERSA CORPORATION , BURK, Mark , DeSANTIS, Grace , MORGAN, Brian , ZHU, Zoulin
发明人： BURK, Mark , DeSANTIS, Grace , MORGAN, Brian , ZHU, Zoulin
IPC分类号： C12P13/00
摘要： The invention provides novel processes for making ethyl-4-cyano-3-hydroxybutyrate, e.g., (R)-ethyl 4-cyano-3-hydroxybutyric acid, and 4-cyano-3-hydroxybutyric acid. The invention provides protocols for making and 4-cyano-3-hydroxybutyric acid and ethyl-4-cyano-3-hydroxybutyrate by whole cell processes, cell lysate processes, "one pot processes" and "multi-pot" processes using a variety of parameters.

4. 发明申请

WO2003106415A2 PROCESSES FOR MAKING (R)-ETHYL 4-CYANO-3-HYDROXYBUTYRIC ACID 审中-公开
标题翻译：制备（R） - 乙基4-氰基-3-羟基丁酸的方法
公开(公告)号：WO2003106415A2
公开(公告)日：2003-12-24
申请号：PCT/US2003/018840
申请日：2003-06-13
申请人： DIVERSA CORPORATION , BURK, Mark , DeSANTIS, Grace , MORGAN, Brian , ZHU, Zoulin
发明人： BURK, Mark , DeSANTIS, Grace , MORGAN, Brian , ZHU, Zoulin
IPC分类号： C07D
CPC分类号： C12P13/004 , C07C253/14 , C07C253/30 , C12P13/002 , C12P13/02 , C12P17/10 , C07C255/16
摘要： The invention provides novel processes for making ethyl-4-cyano-3-hydroxybutyrate, e.g., (R)-ethyl 4-cyano-3-hydroxybutyric acid, and 4-cyano-3-hydroxybutyric acid. The invention provides protocols for making and 4-cyano-3-hydroxybutyric acid and ethyl-4-cyano-3-hydroxybutyrate by whole cell processes, cell lysate processes, "one pot processes" and "multi-pot" processes using a variety of parameters.
摘要翻译：本发明提供了制备4-氰基-3-羟基丁酸乙酯的新方法，例如（R）-4-氰基-3-羟基丁酸乙酯和4-氰基-3-羟基丁酸。本发明提供了通过全细胞过程制备和4-氰基-3-羟基丁酸和4-氰基-3-羟基丁酸乙酯的方案，细胞裂解物方法，“一锅法”和“多锅”方法，使用各种参数。

5. 发明申请

WO2003000909A3 METHODS FOR THE MANUFACTURE OF PURE SINGLE ENANTIOMER COMPOUNDS AND FOR SELECTING ENANTIOSELECTIVE ENZYMES 审中-公开
公开(公告)号：WO2003000909A3
公开(公告)日：2003-01-03
申请号：PCT/US2002/019706
申请日：2002-06-21
申请人： DIVERSA CORPORATION , WEINER, David , HITCHMAN, Tim , ZHAO, Lishan , BURK, Mark , DESANTIS, Grace , HANSON, Sarah, Richardson , MILAN, Aileen , RICHARDSON, Toby , KRETZ, Patti , GREENBURG, William
发明人： WEINER, David , HITCHMAN, Tim , ZHAO, Lishan , BURK, Mark , DESANTIS, Grace , HANSON, Sarah, Richardson , MILAN, Aileen , RICHARDSON, Toby , KRETZ, Patti , GREENBURG, William
IPC分类号： C12Q1/68
摘要： The invention provides biocatalytic methods for the manufacture of pure single enantiomer compounds. The invention provides methods of screening for enzymes which are highly enantioselective or enzymes that can provide any desired stereoisomer of a compound. The invention provides the use of single enantiomer substrates in performing a growth screen of a clonal library to identify highly stereoselective enzymes. In one aspect, methods for screening and identification of enzymes, e.g., transaminases, nitrilases, aldolases, epoxide hydrolases are provided. Methods for the production and screening of gene libraries generated form nucleic acids isolated from more than one organism for enzyme, e.g., transaminase, activities are also provided.

6. 发明申请

WO2003000840A2 NITRILASES 审中-公开
标题翻译：腈
公开(公告)号：WO2003000840A2
公开(公告)日：2003-01-03
申请号：PCT/US2002/015983
申请日：2002-05-15
申请人： DIVERSA CORPORATION , MADDEN, Darcy , DESANTIS, Grace , CHAPLIN, Jennifer, Ann , WEINER, David, Paul , MILAN, Aileen , CHI, Ellen , SHORT, Jay, M. , BURK, Mark
发明人： DESANTIS, Grace , CHAPLIN, Jennifer, Ann , WEINER, David, Paul , MILAN, Aileen , CHI, Ellen , SHORT, Jay, M. , BURK, Mark
IPC分类号： C12N
CPC分类号： C12P13/004 , C12N9/78 , C12P7/42 , C12P13/04 , C12P41/006
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
摘要翻译：本发明涉及腈水解酶和编码腈水解酶的核酸。另外还提供了设计新腈水解酶的方法及其使用方法。在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。

7. 发明申请

WO2003000840A3 NITRILASES 审中-公开
公开(公告)号：WO2003000840A3
公开(公告)日：2003-01-03
申请号：PCT/US2002/015983
申请日：2002-05-15
申请人： DIVERSA CORPORATION , MADDEN, Darcy , DESANTIS, Grace , CHAPLIN, Jennifer, Ann , WEINER, David, Paul , MILAN, Aileen , CHI, Ellen , SHORT, Jay, M. , BURK, Mark
发明人： DESANTIS, Grace , CHAPLIN, Jennifer, Ann , WEINER, David, Paul , MILAN, Aileen , CHI, Ellen , SHORT, Jay, M. , BURK, Mark
IPC分类号： C12N9/00 , C12N9/78 , C12N1/20 , C12N15/00 , C07H21/02 , C12P1/00 , C12P7/00 , C12P7/62 , C07K1/00
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.

8. 发明申请

WO2003000909A2 METHODS FOR THE MANUFACTURE OF PURE SINGLE ENANTIOMER COMPOUNDS AND FOR SELECTING ENANTIOSELECTIVE ENZYMES 审中-公开
标题翻译：纯单纯化合物的制备方法和选择选择性酶的方法
公开(公告)号：WO2003000909A2
公开(公告)日：2003-01-03
申请号：PCT/US2002/019706
申请日：2002-06-21
申请人： DIVERSA CORPORATION , WEINER, David , HITCHMAN, Tim , ZHAO, Lishan , BURK, Mark
发明人： WEINER, David , HITCHMAN, Tim , ZHAO, Lishan , BURK, Mark
IPC分类号： C12P
CPC分类号： C12N15/1086 , C12P41/006
摘要： The invention provides biocatalytic methods for the manufacture of pure single enantiomer compounds. The invention provides methods of screening for enzymes which are highly enantioselective or enzymes that can provide any desired stereoisomer of a compound. The invention provides the use of single enantiomer substrates in performing a growth screen of a clonal library to identify highly stereoselective enzymes. In one aspect, methods for screening and identification of enzymes, e.g., transaminases, nitrilases, aldolases, epoxide hydrolases are provided. Methods for the production and screening of gene libraries generated form nucleic acids isolated from more than one organism for enzyme, e.g., transaminase, activities are also provided.
摘要翻译：本发明提供用于制造纯单一对映异构体化合物的生物催化方法。本发明提供了筛选高度对映选择性的酶的方法或可以提供化合物的任何所需立体异构体的酶的方法。本发明提供单一对映异构体底物在克隆文库的生长筛选中鉴定高度立体选择性酶的用途。在一个方面，提供了用于筛选和鉴定酶的方法，例如转氨酶，腈水解酶，醛缩酶，环氧化物水解酶。还提供了生产和筛选由多于一种生物体分离的酶形成的基因文库的酶，例如转氨酶，活性。

9. 发明申请

WO2003077868A2 SYNTHESIS OF SYNTHONS FOR THE MANUFACTURE OF BIOACTIVE COMPOUNDS 审中-公开
标题翻译：合成生物活性化合物的合成
公开(公告)号：WO2003077868A2
公开(公告)日：2003-09-25
申请号：PCT/US2003/007982
申请日：2003-03-14
申请人： THE SCRIPPS RESEARCH INSTITUTE , DIVERSA CORPORATION , WONG, Chi-Huey , LIU, Junjie , DE SANTIS, Grace , BURK, Mark
发明人： WONG, Chi-Huey , LIU, Junjie , DE SANTIS, Grace , BURK, Mark
IPC分类号： A61K
CPC分类号： C12P19/02 , C12N9/88 , C12P17/06 , C12R1/19
摘要： The present invention is based on the discovery that 2-deoxyribose-5-phosphate aldolase (DERA, EC 4.1.2.4) and variants thereof can be used to catalyze sequential asymmetric aldol reactions between a wide variety of donor and acceptor aldehydes. The reaction products typically contain at least two new stereogenic centers and can be produced in enantiomerically pure form. As such, DERA catalyzed asymmetric aldol chemistry can be exploited to produce synthons for the synthesis of a variety of bioactive molecules.
摘要翻译：本发明基于以下发现：2-脱氧核糖-5-磷酸醛缩酶（DERA，EC 4.1.2.4）及其变体可用于催化多种供体和受体醛之间的顺序不对称醛醇反应。反应产物通常含有至少两个新的立体中心并且可以以对映体纯的形式制备。因此，DERA催化的不对称醛醇化学可以被利用来产生用于合成各种生物活性分子的合成物。

10. 发明申请

WO2003062399A3 BIOCATALYTIC REDUCTION OF NITRO GROUPS 审中-公开
公开(公告)号：WO2003062399A3
公开(公告)日：2003-07-31
申请号：PCT/US2003/002177
申请日：2003-01-23
申请人： DIVERSA CORPORATION , GREENBERG, William , WEINER, David, Paul , ADGER, Brian , BURK, Mark
发明人： GREENBERG, William , WEINER, David, Paul , ADGER, Brian , BURK, Mark
IPC分类号： C12N9/02
摘要： The invention provides methods of discovering nitroreductases and biocatalytic methods for reducing compounds comprising a nitro group.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式