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1. 发明申请

WO2015086839A1 FUMARATE REDUCTASES 审中-公开
标题翻译：减少脂肪
公开(公告)号：WO2015086839A1
公开(公告)日：2015-06-18
申请号：PCT/EP2014/077638
申请日：2014-12-12
申请人： DSM IP ASSETS B.V.
发明人： JONG, DE, René Marcel , ZHAO, Zheng , DULK, DEN, Ben , WINTER, Remko Tsjibbe
IPC分类号： C12N9/02
CPC分类号： C12N9/001 , C12P7/46 , C12Y103/01006
摘要： The present invention relates to a variant polypeptide having fumarate reductase activity, which has modified NADP(H)-dependent and/or NAD(H)-dependent activity as compared with a reference polypeptide having fumarate reductase activity. Such a variant may be overexpressed in a host cell in order to improve production of a dicarboxylic acid.
摘要翻译：本发明涉及具有富马酸酯还原酶活性的变体多肽，其与具有富马酸盐还原酶活性的参照多肽相比具有修饰的NADP（H） - 依赖和/或NAD（H） - 依赖性活性。这种变体可以在宿主细胞中过表达，以改善二羧酸的产生。

2. 发明申请

WO2013076280A1 NUCLEIC ACID ASSEMBLY SYSTEM 审中-公开
标题翻译：核酸组装系统
公开(公告)号：WO2013076280A1
公开(公告)日：2013-05-30
申请号：PCT/EP2012/073532
申请日：2012-11-23
申请人： DSM IP ASSETS B.V.
发明人： ROUBOS, Johannes, Andries , MEIJRINK, Bernard , KERKMAN, Richard , DULK, DEN, Ben
IPC分类号： C12N15/10 , C12N15/52 , C40B40/06 , C40B40/08
CPC分类号： C12N15/1082 , C12N15/1027 , C12N15/1093 , C12N15/52 , C12N15/80 , C12N15/905
摘要： The present invention relates to a method for the preparation of a library of host cells, a plurality of which comprise an assembled polynucleotide at a target locus, which method comprises: (a) providing a plurality of polynucleotides comprising two or more polynucleotide subgroups, wherein: (i) a plurality of polynucleotides in each polynucleotide subgroup comprises sequence encoding a peptide or polypeptide and/or a regulatory sequence; (ii) a plurality of peptides or polypeptides encoded by, or a plurality of regulatory sequences comprised within, each polynucleotide subgroup share an activity and/or function; (iii) at least one polynucleotide subgroup comprises at least two non-identical polynucleotide species; (iv) a plurality of polynucleotides of each polynucleotide subgroup comprises sequence enabling homologous recombination with a plurality of polynucleotides from one or more other polynucleotide subgroups; and (v) a plurality of polynucleotides in two polynucleotide subgroups comprise a nucleotide sequence enabling homologous recombination with a target locus in host cells; and (b) assembling the plurality of polynucleotides at the target locus by homologous recombination in vivo in host cells, thereby to generate a library of host cells, a plurality of which comprise an assembled polynucleotide at the target locus. The assembled polynucleotides may be recovered, thereby to prepare a library of nucleic acids.
摘要翻译：本发明涉及一种用于制备宿主细胞文库的方法，其多个在靶位点包含组装的多核苷酸，该方法包括：（a）提供多个包含两个或多个多核苷酸亚基的多核苷酸，其中（i）每个多核苷酸亚组中的多个多核苷酸包含编码肽或多肽和/或调节序列的序列; （ii）每个多核苷酸亚组中由多个或多个编码的多肽或多肽调节序列共享活性和/或功能; （iii）至少一个多核苷酸亚组包含至少两个不相同的多核苷酸种类; （iv）每个多核苷酸亚组的多个多核苷酸包括能够与来自一个或多个其它多核苷酸亚组的多个多核苷酸进行同源重组的序列; 和（v）两个多核苷酸亚组中的多个多核苷酸包含能够与宿主细胞中的靶基因座进行同源重组的核苷酸序列; 和（b）通过在宿主细胞中的体内同源重组在靶位点组装多个多核苷酸，从而产生宿主细胞文库，其多个包含靶标位点处的组装的多核苷酸。可以回收组装的多核苷酸，从而制备核酸文库。

3. 发明申请

WO2012089613A1 PROCESS TO INCREASE THE PRODUCTION OF A SUCCINYL-COA DERIVED COMPOUND 审中-公开
标题翻译：增加化合物衍生化合物的方法
公开(公告)号：WO2012089613A1
公开(公告)日：2012-07-05
申请号：PCT/EP2011/073765
申请日：2011-12-22
申请人： DSM IP ASSETS B.V. , HANS, Marcus , DULK, DEN, Ben , WU, Liang
发明人： HANS, Marcus , DULK, DEN, Ben , WU, Liang
IPC分类号： C12N9/00 , C12N5/10
CPC分类号： C12N9/93
摘要： This invention relates to a process to improve the production of a succinyl-CoA derived compound by a eukaryotic cell. This method may be advantageously used in the biological production of e.g. adipic acid.
摘要翻译：本发明涉及通过真核细胞改善琥珀酰辅酶A衍生化合物的生产方法。该方法可有利地用于生物生产中。己二酸。

4. 发明公开

EP2783000A1 NUCLEIC ACID ASSEMBLY SYSTEM 审中-公开
标题翻译：系统对于核酸安排
公开(公告)号：EP2783000A1
公开(公告)日：2014-10-01
申请号：EP12788597.8
申请日：2012-11-23
申请人： DSM IP Assets B.V.
发明人： ROUBOS, Johannes, Andries , MEIJRINK, Bernard , KERKMAN, Richard , DULK, DEN, Ben
IPC分类号： C12N15/10 , C12N15/52 , C40B40/06 , C40B40/08
CPC分类号： C12N15/1082 , C12N15/1027 , C12N15/1093 , C12N15/52 , C12N15/80 , C12N15/905
摘要： The present invention relates to a method for the preparation of a library of host cells, a plurality of which comprise an assembled polynucleotide at a target locus, which method comprises: (a) providing a plurality of polynucleotides comprising two or more polynucleotide subgroups, wherein: (i) a plurality of polynucleotides in each polynucleotide subgroup comprises sequence encoding a peptide or polypeptide and/or a regulatory sequence; (ii) a plurality of peptides or polypeptides encoded by, or a plurality of regulatory sequences comprised within, each polynucleotide subgroup share an activity and/or function; (iii) at least one polynucleotide subgroup comprises at least two non-identical polynucleotide species; (iv) a plurality of polynucleotides of each polynucleotide subgroup comprises sequence enabling homologous recombination with a plurality of polynucleotides from one or more other polynucleotide subgroups; and (v) a plurality of polynucleotides in two polynucleotide subgroups comprise a nucleotide sequence enabling homologous recombination with a target locus in host cells; and (b) assembling the plurality of polynucleotides at the target locus by homologous recombination in vivo in host cells, thereby to generate a library of host cells, a plurality of which comprise an assembled polynucleotide at the target locus. The assembled polynucleotides may be recovered, thereby to prepare a library of nucleic acids.

5. 发明授权

EP3080251B1 FUMARATE REDUCTASES 有权
公开(公告)号：EP3080251B1
公开(公告)日：2020-03-04
申请号：EP14833137.4
申请日：2014-12-12
申请人： DSM IP Assets B.V.
发明人： JONG, DE, René Marcel , ZHAO, Zheng , DULK, DEN, Ben , WINTER, Remko Tsjibbe
IPC分类号： C12P7/46 , C12N9/02

6. 发明公开

EP3080251A1 FUMARATE REDUCTASES 有权
公开(公告)号：EP3080251A1
公开(公告)日：2016-10-19
申请号：EP14833137.4
申请日：2014-12-12
申请人： DSM IP Assets B.V.
发明人： JONG, DE, René Marcel , ZHAO, Zheng , DULK, DEN, Ben , WINTER, Remko Tsjibbe
IPC分类号： C12N9/02
摘要： The present invention relates to a variant polypeptide having fumarate reductase activity, which has modified NADP(H)-dependent and/or NAD(H)-dependent activity as compared with a reference polypeptide having fumarate reductase activity. Such a variant may be overexpressed in a host cell in order to improve production of a dicarboxylic acid.
摘要翻译：本发明涉及具有延胡索酸还原酶活性的变体多肽，与具有延胡索酸还原酶活性的参考多肽相比，其具有修饰的NADP（H） - 依赖性和/或NAD（H） - 依赖性活性。这种变体可以在宿主细胞中过表达以改善二羧酸的生产。

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