会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 3. 发明申请
    • TRANSCRIPTION FACTORS
    • 转录因子
    • WO2006051418A2
    • 2006-05-18
    • PCT/IB2005/003600
    • 2005-11-10
    • DANISCO A/SNIKOLAEV, IgorMADRID, Susan, Mampusti
    • NIKOLAEV, IgorMADRID, Susan, Mampusti
    • C07K14/37C12N15/63C12N15/80C12N1/20C12N1/14C12N5/10
    • C07K14/38C12N15/80C12N2501/60
    • The present invention relates to an isolated nucleic acid sequence coding for a PntR transcription factor comprising a nucleotide sequence that is the same as, or is complementary to, or contains any suitable codon substitutions for any of those of SEQ ID NOs: 1, 3 or 5 or comprises a sequence which has at least 60% sequence homology with any of SEQ ID NOs: 1, 3 or 5, as well as corresponding polypeptides, vector systems and host cells comprising the same. In particular, the invention relates to sequences which are obtained from an Aspergillus, Trichoderma or Penicillium cell. The invention further relates to methods for disrupting PntR expression in a cell as well as methods for expression or production of a POI in a host cell in which PntR expression has been disrupted.
    • 本发明涉及编码PntR转录因子的分离的核酸序列,所述核酸序列包含与SEQ ID NO:1,3或任何SEQ ID NO:1或SEQ ID NO:3中任一个的任何合适的密码子取代相同或互补的核苷酸序列 或包含与SEQ ID NO:1,3或5中任一个具有至少60%序列同源性的序列,以及相应的多肽,载体系统和包含该序列的宿主细胞。 特别地,本发明涉及从曲霉属,木霉属或青霉属细胞获得的序列。 本发明还涉及破坏细胞中PntR表达的方法以及在PntR表达已被破坏的宿主细胞中表达或产生POI的方法。