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1. 发明申请

WO2006008653A2 LIPOLYTIC ENZYME USES THEREOF IN THE FOOD INDUSTRY 审中-公开
标题翻译：蛋白
公开(公告)号：WO2006008653A2
公开(公告)日：2006-01-26
申请号：PCT/IB2005/002602
申请日：2005-07-18
申请人： DANISCO A/S , MIASNIKOV, Andrei , SØE, Jørn, Borch , MIKKELSEN, Jørn, Dalgaard , POVELAINEN, Mira , PITKANEN, Virve
发明人： MIASNIKOV, Andrei , SØE, Jørn, Borch , MIKKELSEN, Jørn, Dalgaard , POVELAINEN, Mira , PITKANEN, Virve
IPC分类号： C11B3/00 , C11C3/00 , C07F9/10 , C12N15/55 , C12N9/20 , C12P7/64 , A23C9/13 , A21D2/26
CPC分类号： C12N9/1029 , A21D8/042 , A23J7/00 , C11B3/003 , C12N9/20 , C12P7/6418 , C12P7/6445 , C12P7/6481 , C12Y301/01003
摘要： The invention also encompases the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein said lipolytic enzyme is capable of hydrolysing a glycolipid or a phospholipid or transferring an acyl group from a glycolipid or phospholipids to a acyl acceptor. Preferably, the lipolytic enzyme for use in these methods and uses comprises an amino acid sequence as shown in any one of SEQ. ID. No.s 4, 5, 7, 8, 12, 14 or 16 or an amino acid sequence having at least 70% identity therewith or comprises a nucleotide sequence shown as SEQ ID No. 3, 6, 9, 13, 15 or 17 or a nulceotide seqeunce which has at least 70% identity therewith. The present invention also relates to a lipolytic enzyme capable of hydrolysing at least a galactolipid or capable of transferring an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Steptomyces species and includes a lipolytic enzyme comprising an amino acid sequence as shown in SEQ ID No. 4 or an amino acid sequence which has at least 60% identity thereto. The enzyme may be encoded by a nucleic acid selected from the group consisting of: a) a nucleic acid comprising a nucleotide sequence shown in SEQ ID No. 3; b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3 by the degeneration of the genetic code; and c) a nucleic acid comprising a nucleotide sequence which has at least 70% identity with the nucleotide sequence shown in SEQ ID No. 3.
摘要翻译：本发明还包括使用可从各种方法获得的脂肪分解酶：链霉菌，棒状杆菌和热嗜热菌，其中所述脂肪分解酶能够水解糖脂或磷脂或从糖脂转移酰基或磷脂转化为酰基受体。优选地，用于这些方法和用途的脂肪分解酶包含SEQ ID NO：1中任一项所示的氨基酸序列。 ID。第4,5,7,8,12,14或16号氨基酸序列或与其具有至少70％同一性的氨基酸序列，或包含SEQ ID No.3,6,13,13,15或17所示的核苷酸序列或与其具有至少70％同一性的nucceotide seqonunce。本发明还涉及能够至少水解半乳糖脂或能够将酰基从半乳糖脂转移至一种或多种酰基受体底物的脂解酶，其中所述酶可从步态物种获得，并且包括脂肪分解酶，其包含氨基如SEQ ID No.4所示的酸序列或与其具有至少60％同一性的氨基酸序列。该酶可以由选自以下的核酸编码：a）包含SEQ ID No.3所示核苷酸序列的核酸; b）通过遗传密码的退化与SEQ ID No.3的核苷酸序列相关的核酸; 和c）包含与SEQ ID No.3所示核苷酸序列具有至少70％同一性的核苷酸序列的核酸。

2. 发明申请

WO2006123254A2 METHOD 审中-公开
标题翻译：方法
公开(公告)号：WO2006123254A2
公开(公告)日：2006-11-23
申请号：PCT/IB2006/001841
申请日：2006-05-15
申请人： DANISCO A/S , KUMAR, Vijay , MIASNIKOV, Andrei
发明人： KUMAR, Vijay , MIASNIKOV, Andrei
IPC分类号： C12Q1/42 , C07F9/09 , A61K31/661 , A61K31/6615
CPC分类号： C12Q1/42 , C07F9/12
摘要： There is provided a method for the detection of phytase activity in a sample, which comprises bringing into association a phytase substrate and said sample, and measuring the level of of an organic metabolite of said phytase substrate.
摘要翻译：提供了一种用于检测样品中植酸酶活性的方法，其包括使肌醇六磷酸酶底物和所述样品结合，并测量所述植酸酶底物的有机代谢物的水平。

3. 发明申请

WO2006038128A3 CITROBACTER FREUNDII PHYTASE AND HOMOLOGUES 审中-公开
公开(公告)号：WO2006038128A3
公开(公告)日：2006-04-13
申请号：PCT/IB2005/003660
申请日：2005-10-04
申请人： DANISCO A/S , MIASNIKOV, Andrei , KUMAR, Vijay , KENSCH, Oliver , PELLANGAHR, Klaus , LEUTHNER, Birgitta , KETTLING, Ulrich , KOLTERMANN, Andre
发明人： MIASNIKOV, Andrei , KUMAR, Vijay , KENSCH, Oliver , PELLANGAHR, Klaus , LEUTHNER, Birgitta , KETTLING, Ulrich , KOLTERMANN, Andre
IPC分类号： C12N9/16 , C12N15/55 , C12N5/10 , A23L1/03 , A23K1/165 , C12N1/14 , C12N1/20 , C12R1/01
摘要： The present invention relates to enzymes and processes. In particular, there is described an isolated polypeptide comprising the amino acid sequence corresponding to Citrobacter freundii phytase or a homologue, a modified form, a functional equivalent or an effective fragment thereof. There is also described a host cell transformed or transfected with a nucleic acid encoding a bacterial phytase enzyme or a modified form as well as the use of such a phytase or modified form in food or animal feed.

4. 发明申请

WO2010150213A1 PROTEIN 审中-公开
标题翻译：蛋白
公开(公告)号：WO2010150213A1
公开(公告)日：2010-12-29
申请号：PCT/IB2010/052868
申请日：2010-06-23
申请人： DANISCO A/S , MIASNIKOV, Andrei , BOTT, Richard, R , SØRENSEN, Jens, Frisbæk
发明人： MIASNIKOV, Andrei , BOTT, Richard, R , SØRENSEN, Jens, Frisbæk
IPC分类号： C12N9/18 , C12N9/20 , A23L1/015 , C12N15/55
CPC分类号： C12N9/20 , A21D8/042 , C12N9/18 , C12Y301/01003 , C12Y301/01004 , C12Y301/01026 , C12Y301/01032
摘要： The present invention relates to a method for preparing a variant lipolytic enzyme comprising expressing in a host organism a nucleotide sequence which has at least 90% identity with a nucleotide sequence encoding a fungal lipolytic enzyme and comprises at at least one modification at a position which corresponds in the encoded amino acid sequence to a) the introduction of at least one glycosylation site (or one additional glycosylation site) in the amino acid sequence compared with the original fungal lipolytic enzyme; b) the introduction of at least one amino acid at a surface position on the polypeptide and at a location in an external loop distal to the active site (catalytic triad) of the enzyme which is more hydrophilic (compared with the original amino acid); or c) a substitution or insertion at one or more of positions 33, 63, 78, 190, 305, 306 or 320 or a deletion at one or more positions 311-312 or 307-319, wherein each amino acid position corresponds to the position of the amino acid sequence when aligned with SEQ ID No. 2; wherein when the nucleotide sequence has at least 90% identity with a nucleotide sequence encoding the fungal lipolytic enzyme shown in SEQ ID No. 22 or SEQ ID No. 23 the modification is not a substitution at position 63 and the deletion is not at position 311 -312. Preferably the nucleotide sequence has at least 90% identity with SEQ ID No. 1. The invention also relates to polypeptide produced by the method and to novel nucleic acids.
摘要翻译：本发明涉及一种制备变体脂肪分解酶的方法，其包括在宿主生物体中表达与编码真菌脂肪分解酶的核苷酸序列具有至少90％同一性的核苷酸序列，并且包括在对应的位置处的至少一个修饰在编码的氨基酸序列中a）与原始真菌脂肪分解酶相比，在氨基酸序列中引入至少一个糖基化位点（或另外一个糖基化位点）; b）在多肽的表面位置和远离更亲水的酶（与原始氨基酸相比）的活性位点（催化三单元组）的外部环路的位置处引入至少一个氨基酸; 或c）在位置33,63,78,190,305,306或320中的一个或多个处的取代或插入或在一个或多个位置311-312或307-319处的缺失，其中每个氨基酸位置对应于当与SEQ ID No.2对齐时，氨基酸序列的位置; 其中当所述核苷酸序列与编码SEQ ID No.22或SEQ ID No.23所示的真菌脂肪分解酶的核苷酸序列具有至少90％同一性时，所述修饰不是位置63处的取代，并且缺失不在位置311 -312。优选地，核苷酸序列与SEQ ID No.1具有至少90％的同一性。本发明还涉及通过该方法产生的多肽和新的核酸。

5. 发明申请

WO2006043178A3 ENZYMES 审中-公开
公开(公告)号：WO2006043178A3
公开(公告)日：2006-04-27
申请号：PCT/IB2005/003376
申请日：2005-10-17
申请人： DANISCO A/S , MIASNIKOV, Andrei , KUMAR, Vijay , KENSCH, Oliver , PELLENGAHR, Klaus , LEUTHNER, Birgitta , KETTLING, Ulrich , KOLTERMANN, Andre
发明人： MIASNIKOV, Andrei , KUMAR, Vijay , KENSCH, Oliver , PELLENGAHR, Klaus , LEUTHNER, Birgitta , KETTLING, Ulrich , KOLTERMANN, Andre
IPC分类号： C12N9/16 , C12N15/55 , C12N15/11 , C12P21/02 , C12N1/20 , A23K1/165
摘要： The present invention relates to enzymes and processes. In particular, there is described a host cell transformed or transfected with a nucleic acid encoding a bacterial phytase enzyme and variants thereof.

6. 发明申请

WO2006038062A1 MICROBIAL PHYTASE AS SUPPLEMENT TO FOOD OR FODDER 审中-公开
标题翻译：微生物作为食品或食品的补充
公开(公告)号：WO2006038062A1
公开(公告)日：2006-04-13
申请号：PCT/IB2005/000598
申请日：2005-02-15
申请人： DANISCO A/S , MIASNIKOV, Andrei , KUMAR, Vijay , KENSCH, Oliver , PELLENGAHR, Klaus , LEUTHNER, Birgitta , KETTLING, Ulrich , KOLTERMANN, Andre
发明人： MIASNIKOV, Andrei , KUMAR, Vijay , KENSCH, Oliver , PELLENGAHR, Klaus , LEUTHNER, Birgitta , KETTLING, Ulrich , KOLTERMANN, Andre
IPC分类号： C12N9/16
CPC分类号： C12N9/16
摘要： The present invention relates to enzymes and processes. In particular, there is described an isolated polypeptide comprising the amino acid sequence corresponding to Citrobacter freundii phytase or a homologue, a modified form, a functional equivalent or an effective fragment thereof. There is also described a host cell transformed or txansfected with a nucleic acid encoding a bacterial phytase enzyme or a modified form as well as the use of such a phytase or modified form in food or animal feed.
摘要翻译：本发明涉及酶和方法。特别地，描述了分离的多肽，其包含对应于弗氏柠檬酸杆菌植酸酶的氨基酸序列或其同源物，修饰形式，功能等同物或其有效片段。还描述了用编码细菌植酸酶或修饰形式的核酸转化或转染的宿主细胞，以及在食物或动物饲料中使用这种植酸酶或修饰形式。

7. 发明申请

WO2010107560A2 FUNGAL CUTINASE FROM MAGNAPORTHE GRISEA 审中-公开
标题翻译：来自MAGNAPORTHE GRISEA的真菌
公开(公告)号：WO2010107560A2
公开(公告)日：2010-09-23
申请号：PCT/US2010/025254
申请日：2010-02-24
申请人： DANISCO US INC. , ADAMS, Christian, D. , MIASNIKOV, Andrei
发明人： ADAMS, Christian, D. , MIASNIKOV, Andrei
IPC分类号： C07K14/37 , C11D3/00 , C12N9/10 , C12N9/14
CPC分类号： C12N9/18 , C11D3/38627 , C11D3/38681 , C12P7/6454 , C12Y301/01074
摘要： Described are compositions and methods relating to a fungal cutinase cloned from Magnaporthe grisea , polynucleotides encoding the cutinase, and methods of use thereof. The compositions and methods have particular application in detergent cleaning compositions and synthesis reactions.
摘要翻译：描述了与从Magnaporthe grisea克隆的真菌角质酶相关的组合物和方法，编码角质酶的多核苷酸及其使用方法。该组合物和方法特别适用于洗涤剂清洁组合物和合成反应。

8. 发明申请

WO2009114380A1 EXPRESSION OF CATALASE IN TRICHODERMA 审中-公开
标题翻译：催乳素在TRICHODERMA中的表达
公开(公告)号：WO2009114380A1
公开(公告)日：2009-09-17
申请号：PCT/US2009/036146
申请日：2009-03-05
申请人： DANISCO US INC., GENENCOR DIVISION , DODGE, Timothy C. , HOFFMAN, Katherine Marie , MIASNIKOV, Andrei , WARD, Michael
发明人： DODGE, Timothy C. , HOFFMAN, Katherine Marie , MIASNIKOV, Andrei , WARD, Michael
IPC分类号： C12N9/08 , C12P21/02 , C12R1/885
CPC分类号： C12N9/0065 , C12P3/00 , C12Y111/01006
摘要： The invention provides methods for expression of a catalase enzyme in a Trichoderma host cell. In one embodiment, the catR gene from Aspergillus niger is expressed in Trichoderma reesei , resulting in improved yields of catalase enzyme in comparison with expression of catR in A. niger .
摘要翻译：本发明提供了在木霉属宿主细胞中表达过氧化氢酶的方法。在一个实施方案中，来自黑曲霉的catR基因在里氏木霉中表达，与黑曲霉中catR的表达相比，导致过氧化氢酶的产量提高。

9. 发明申请

WO2012054554A2 THERMOSTABLE TRICHODERMA CELLULASE 审中-公开
标题翻译：耐热TRICHODERMA纤维素酶
公开(公告)号：WO2012054554A2
公开(公告)日：2012-04-26
申请号：PCT/US2011/056810
申请日：2011-10-19
申请人： DANISCO US INC. , MIASNIKOV, Andrei , SCHELLE, Michael , WARD, Michael
发明人： MIASNIKOV, Andrei , SCHELLE, Michael , WARD, Michael
IPC分类号： C12P21/06 , C12N1/00 , C07D491/048
CPC分类号： C12N9/2437 , C07K14/37 , C12N15/80 , C12P21/02 , C12Y302/01004
摘要： Described are compositions and methods relating to the thermostable fungal cellulase enzyme, EGV, and Trichoderma host cells having a modification comprising or consisting essentially of disruption or deletion of nucleotide(s) for expression of this cellulose, whereby EGV expression is prevented.
摘要翻译：描述了与热稳定性真菌纤维素酶，EGV和木霉属宿主细胞相关的组合物和方法，其具有包含或基本上由此表达该纤维素的核苷酸的或由其组成的修饰，由此可以预防EGV表达。

10. 发明申请

WO2009132220A2 ISOPRENE SYNTHASE VARIANTS FOR IMPROVED MICROBIAL PRODUCTION OF ISOPRENE 审中-公开
标题翻译：用于改善微生物生产异烟肼的异丙肾上腺素变异体
公开(公告)号：WO2009132220A2
公开(公告)日：2009-10-29
申请号：PCT/US2009/041581
申请日：2009-04-23
申请人： DANISCO US INC. , THE GOODYEAR TIRE & RUBBER COMPANY , CERVIN, Marguerite, A. , WHITED, Gregory, M. , MIASNIKOV, Andrei , PERES, Caroline, M. , WEYLER, Walter , WELLS, Derek, H. , BOTT, Richard, R.
发明人： CERVIN, Marguerite, A. , WHITED, Gregory, M. , MIASNIKOV, Andrei , PERES, Caroline, M. , WEYLER, Walter , WELLS, Derek, H. , BOTT, Richard, R.
IPC分类号： C12N9/88 , C12N15/52 , C12N5/10
CPC分类号： C12N9/88 , C12P5/007 , C12Y402/03027
摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
摘要翻译：本发明提供包含至少一种具有改进的催化活性和/或溶解度的异戊二烯合酶的方法和组合物。特别地，本发明提供用于在微生物宿主细胞中增加异戊二烯生产的变体植物异戊二烯合酶。本发明的生物合成生产的异戊二烯可用于制造橡胶和弹性体。

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