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1. 发明申请

WO2010150213A1 PROTEIN 审中-公开
标题翻译：蛋白
公开(公告)号：WO2010150213A1
公开(公告)日：2010-12-29
申请号：PCT/IB2010/052868
申请日：2010-06-23
申请人： DANISCO A/S , MIASNIKOV, Andrei , BOTT, Richard, R , SØRENSEN, Jens, Frisbæk
发明人： MIASNIKOV, Andrei , BOTT, Richard, R , SØRENSEN, Jens, Frisbæk
IPC分类号： C12N9/18 , C12N9/20 , A23L1/015 , C12N15/55
CPC分类号： C12N9/20 , A21D8/042 , C12N9/18 , C12Y301/01003 , C12Y301/01004 , C12Y301/01026 , C12Y301/01032
摘要： The present invention relates to a method for preparing a variant lipolytic enzyme comprising expressing in a host organism a nucleotide sequence which has at least 90% identity with a nucleotide sequence encoding a fungal lipolytic enzyme and comprises at at least one modification at a position which corresponds in the encoded amino acid sequence to a) the introduction of at least one glycosylation site (or one additional glycosylation site) in the amino acid sequence compared with the original fungal lipolytic enzyme; b) the introduction of at least one amino acid at a surface position on the polypeptide and at a location in an external loop distal to the active site (catalytic triad) of the enzyme which is more hydrophilic (compared with the original amino acid); or c) a substitution or insertion at one or more of positions 33, 63, 78, 190, 305, 306 or 320 or a deletion at one or more positions 311-312 or 307-319, wherein each amino acid position corresponds to the position of the amino acid sequence when aligned with SEQ ID No. 2; wherein when the nucleotide sequence has at least 90% identity with a nucleotide sequence encoding the fungal lipolytic enzyme shown in SEQ ID No. 22 or SEQ ID No. 23 the modification is not a substitution at position 63 and the deletion is not at position 311 -312. Preferably the nucleotide sequence has at least 90% identity with SEQ ID No. 1. The invention also relates to polypeptide produced by the method and to novel nucleic acids.
摘要翻译：本发明涉及一种制备变体脂肪分解酶的方法，其包括在宿主生物体中表达与编码真菌脂肪分解酶的核苷酸序列具有至少90％同一性的核苷酸序列，并且包括在对应的位置处的至少一个修饰在编码的氨基酸序列中a）与原始真菌脂肪分解酶相比，在氨基酸序列中引入至少一个糖基化位点（或另外一个糖基化位点）; b）在多肽的表面位置和远离更亲水的酶（与原始氨基酸相比）的活性位点（催化三单元组）的外部环路的位置处引入至少一个氨基酸; 或c）在位置33,63,78,190,305,306或320中的一个或多个处的取代或插入或在一个或多个位置311-312或307-319处的缺失，其中每个氨基酸位置对应于当与SEQ ID No.2对齐时，氨基酸序列的位置; 其中当所述核苷酸序列与编码SEQ ID No.22或SEQ ID No.23所示的真菌脂肪分解酶的核苷酸序列具有至少90％同一性时，所述修饰不是位置63处的取代，并且缺失不在位置311 -312。优选地，核苷酸序列与SEQ ID No.1具有至少90％的同一性。本发明还涉及通过该方法产生的多肽和新的核酸。

2. 发明申请

WO2011045629A1 LIPID ACYLTRANSFERASE PROTEINS AND METHODS OF MAKING THEM 审中-公开
标题翻译：脂质过氧化物酶蛋白及其制备方法
公开(公告)号：WO2011045629A1
公开(公告)日：2011-04-21
申请号：PCT/IB2009/054535
申请日：2009-10-15
申请人： DANISCO A/S , BRUNSTEDT, Janne , SØRENSEN, Jens, Frisbæk , SØE, Jørn, Borch , VEDEL, Charlotte, Johansen , WITTSCHIEBEN, Birgitte, Ø. , MIKKELSEN, Jørn, Dalgaard , POULSEN, Charlotte, Horsmans , JENSEN, Lene, B. , MILLER, Lone, B. , LARSEN, Morten, K , LORENTZEN, Rikke, H. , THOUDAHL, Charlotte, R. , CERVIN, Marguerite, A. , BOTT, Richard, R.
发明人： BRUNSTEDT, Janne , SØRENSEN, Jens, Frisbæk , SØE, Jørn, Borch , VEDEL, Charlotte, Johansen , WITTSCHIEBEN, Birgitte, Ø. , MIKKELSEN, Jørn, Dalgaard , POULSEN, Charlotte, Horsmans , JENSEN, Lene, B. , MILLER, Lone, B. , LARSEN, Morten, K , LORENTZEN, Rikke, H. , THOUDAHL, Charlotte, R. , CERVIN, Marguerite, A. , BOTT, Richard, R.
IPC分类号： C12N9/18 , C12N15/75 , C12N1/21
CPC分类号： C12N9/1029 , A21D8/02 , A23V2002/00 , C12N15/00
摘要： In one aspect of the present invention there is provided a method for preparing a variant lipid acyltransferase enzyme comprising expressing in a host organism a nucleotide sequence which has at least 90% identity with a nucleotide sequence encoding a parent lipid acyltransferase and comprises at least one modification (suitably at least two modifications) at a position(s) which corresponds in the encoded amino acid sequence to an amino acid(s) located in a) the canyon region of the enzyme (i.e. preferably amino acid residues 31, 27, 85, 86, 119, and 120); and/or b) insertion site 1 (i.e. amino acid residues 22-36) and/or c) insertion site 2 (i.e. amino acid residues 74-88), wherein the canyon region, insertion site 1 and/or insertion site 2 are defined as that region which when aligned based on primary or tertiary structure corresponds to the canyon region, insertion site 1 or insertion site 2 (or the corresponding amino acid residues taught above) of the enzyme shown herein as SEQ ID No. 16 or 6. Preferable modifications are one or more of the following L3 IQ, H, N, T, F, Y or C (preferably L31 Q); M27R, G, H, K, Y, D, N, V, C, Q, L, E, S or F (preferably M27V); V85H, R5 D or E; I86R,Y, S, V, I, As T, M, F, C or L (preferably I86S or A); Al 19T or I; Y120K or E; W122S, L or A (preferably W122L); E201R; Q245S; F235A or V; W232G or S; and/or A236G or E.
摘要翻译：在本发明的一个方面，提供了一种制备变体脂质酰基转移酶的方法，包括在宿主生物体中表达与编码亲本脂质酰基转移酶的核苷酸序列具有至少90％同一性的核苷酸序列，并且包含至少一个修饰（适当地至少两个修饰）在编码的氨基酸序列中对应的位置与位于a）谷氨酸区域的氨基酸（即，优选氨基酸残基31,27,85， 86,119和120）; 和/或b）插入位点1（即氨基酸残基22-36）和/或c）插入位点2（即氨基酸残基74-88），其中峡谷区，插入位点1和/或插入位点2是定义为当基于一级或三级结构对准的区域对应于如本文所示的酶的SEQ ID No.16或6的峡谷区，插入位点1或插入位点2（或上文所述的相应的氨基酸残基）。优选的修饰是以下L3 IQ，H，N，T，F，Y或C（优选L31Q）中的一种或多种; M27R，G，H，K，Y，D，N，V，C，Q，L，E，S或F（优选M27V）; V85H，R5 D或E; I86R，Y，S，V，I，As T，M，F，C或L（优选I86S或A）; Al 19T或I; Y120K或E; W122S，L或A（优选W122L）; E201R; Q245S; F235A或V; W232G或S; 和/或A236G或E.

3. 发明申请

WO2010072226A1 POLYPEPTIDES WITH XYLANASE ACTIVITY 审中-公开
标题翻译：具有XYLANASE活性的多糖
公开(公告)号：WO2010072226A1
公开(公告)日：2010-07-01
申请号：PCT/DK2009/050353
申请日：2009-12-23
申请人： Danisco A/S , SIBBESEN, Ole , SØRENSEN, Jens, Frisbæk
发明人： SIBBESEN, Ole , SØRENSEN, Jens, Frisbæk
IPC分类号： C12N9/24
CPC分类号： C12N9/248
摘要： Polypeptides with xylanase activity modified to increase bran solubilisation and/or xylanase activity. The modification comprises modification of one or more amino acids in position 113, 122 or 175 in combination with one or more further amino acid modifications in position 11, 12, 13, 34, 54, 77, 81, 82, 104, 110, 113, 118, 122, 141, 154, 159, 162, 164, 166, 175 or 179, wherein the positions are determined as the position corresponding to the position of Bacillus subtilis xylanase (SEQ ID NO 1).
摘要翻译：具有木聚糖酶活性的多肽被修饰以增加麸质增溶和/或木聚糖酶活性。该修饰包括与位置11,12,13,44,57,78,81,82,104,110,113中的一个或多个其它氨基酸修饰相结合的位置113,122或175中的一个或多个氨基酸的修饰，118,122,141,154,159,162,164,166,175或179，其中所述位置被确定为对应于枯草芽孢杆菌木聚糖酶（SEQ ID NO 1）的位置的位置。

4. 发明申请

WO2003020923A1 XYLANASE VARIANTS 审中-公开
公开(公告)号：WO2003020923A1
公开(公告)日：2003-03-13
申请号：PCT/IB2002/003797
申请日：2002-08-30
申请人： DANISCO A/S , SIBBESEN, Ole , SØRENSEN, Jens, Frisbæk
发明人： SIBBESEN, Ole , SØRENSEN, Jens, Frisbæk
IPC分类号： C12N9/24
CPC分类号： C12N9/2482 , C12Y302/01008
摘要： The present invention relates to a variant xylanase polypeptide, or fragment thereof having xylanase activity, comprising one or more amino acid modifications such that the polypeptide or fragment thereof has an altered sensitivity to a xylanase inhibitor and has an altered thermo-sensitivity as compared with the parent xylanase enzyme.
摘要翻译：本发明涉及具有木聚糖酶活性的变体木聚糖酶多肽或其片段，其包含一个或多个氨基酸修饰，使得多肽或其片段对木聚糖酶抑制剂具有改变的敏感性，并且与亲本木聚糖酶。

5. 发明申请

WO2011124678A1 BRAN MODIFICATION 审中-公开
标题翻译：品牌修改
公开(公告)号：WO2011124678A1
公开(公告)日：2011-10-13
申请号：PCT/EP2011/055502
申请日：2011-04-08
申请人： DANISCO A/S , SØRENSEN, Jens, Frisbæk
发明人： SØRENSEN, Jens, Frisbæk
IPC分类号： A23L1/10
CPC分类号： C12Y302/01008 , A23L7/107 , A23L7/115 , C12Y302/01004 , C12Y302/01015 , C12Y302/01021 , C12Y302/01055 , C12Y302/01089 , C12Y302/01091
摘要： The present invention relates to a method for the modification of cereal bran, a method for the production of a modified cereal bran product as well as the use of such products in the production of food products.
摘要翻译：本发明涉及一种改性谷糠的方法，一种改性谷物麸产品的生产方法以及这些产品在食品生产中的应用。

6. 发明申请

WO2011114251A1 FOODSTUFF 审中-公开
标题翻译：食品
公开(公告)号：WO2011114251A1
公开(公告)日：2011-09-22
申请号：PCT/IB2011/050869
申请日：2011-03-01
申请人： DANISCO A/S , SØE, Jørn, Borch , SØRENSEN, Jens, Frisbæk , JENSEN, Lene, Bojsen
发明人： SØE, Jørn, Borch , SØRENSEN, Jens, Frisbæk , JENSEN, Lene, Bojsen
IPC分类号： A21D2/18 , A21D8/04 , A23L1/015 , A23L1/30
CPC分类号： A23D7/005 , A21D2/183 , A21D2/186 , A21D8/042 , A23D9/007 , A23L5/25 , A23L29/06 , A23V2002/00 , C12Y203/01 , C12Y204/01019 , C12Y301/01003 , C12Y301/01004 , C12Y301/01026 , C12Y301/01032 , A23V2250/5112
摘要： A foodstuff containing an effective amount of: (a) a lipolytic enzyme capable of acting on a lipid substrate to liberate a free fatty acid molecule; and (b) a cyclodextrin; is disclosed. Methods of producing the foodstuff, as well as uses of a cyclodextrin for decreasing the free fatty acid content of a foodstuff incorporating a lipolytic enzyme, are also disclosed.
摘要翻译：一种含有有效量的食品：（a）能够作用于脂质底物以释放游离脂肪酸分子的脂肪分解酶; 和（b）环糊精; 被披露。还公开了生产食品的方法以及环糊精用于降低含有脂肪分解酶的食品的游离脂肪酸含量的用途。

7. 发明申请

WO2010143149A3 METHOD FOR TREATING PYROPHEOPHYTIN-CONTAINING COMPOSITIONS 审中-公开
公开(公告)号：WO2010143149A3
公开(公告)日：2010-12-16
申请号：PCT/IB2010/052581
申请日：2010-06-10
申请人： DANISCO A/S , SØE, Jørn, Borch , POULSEN, Charlotte, Horsmans , ZARGAHI, Masoud, Rajabi , SØRENSEN, Jens, Frisbæk , JØRGENSEN, Tina , BRUNSTEDT, Janne , MIKKELSEN, Rene , MADRID, Susan, Mampusti
发明人： SØE, Jørn, Borch , POULSEN, Charlotte, Horsmans , ZARGAHI, Masoud, Rajabi , SØRENSEN, Jens, Frisbæk , JØRGENSEN, Tina , BRUNSTEDT, Janne , MIKKELSEN, Rene , MADRID, Susan, Mampusti
IPC分类号： C12N9/18 , A23K1/165 , A23L1/277 , C11B3/00
摘要： The present invention provides methods and uses for treating pyropheophytin-containing compositions, particularly in order to remove pyropheophytin therefrom. The compositions are typically plant, algal, or bacterial derived products such as vegetable oils. The method comprises a step of contacting the compositions with an enzyme having pyropheophytinase activity. Also provided are related apparatus and products for performing such methods and uses.

8. 发明申请

WO2010072225A1 POLYPEPTIDES WITH XYLANASE ACTIVITY 审中-公开
标题翻译：具有XYLANASE活性的多糖
公开(公告)号：WO2010072225A1
公开(公告)日：2010-07-01
申请号：PCT/DK2009/050352
申请日：2009-12-23
申请人： Danisco A/S , SIBBESEN, Ole , SØRENSEN, Jens, Frisbæk
发明人： SIBBESEN, Ole , SØRENSEN, Jens, Frisbæk
IPC分类号： C12N9/24
CPC分类号： C12N9/2482 , C12N9/2434 , Y02E50/16
摘要： Polypeptides with xylanase activity modified to increase bran solubilization and/or xylanase activity. The modification comprises modification of one or more amino acids in position 12 or 13 in combination with one or more further amino acid modifications in position 15, 34, 54, 77, 81, 82, 99, 104, 110, 113, 114, 118, 122, 141, 154, 159, 162, 164, 166, 175 or 179, wherein the positions are determined as the position corresponding the position of Bacillus subtilis xylanase (SEQ ID NO 1).
摘要翻译：具有木聚糖酶活性的多肽被修饰以增加麸增溶和/或木聚糖酶活性。修饰包括在位置12或13上与位置15,34,54,77,81,82,99,104,110,113,114,118中的一个或多个其它氨基酸修饰的一个或多个氨基酸的修饰，122,141,154,159,162,164,166,175或179，其中所述位置被确定为与枯草芽孢杆菌木聚糖酶（SEQ ID NO 1）的位置相对应的位置。

9. 发明公开

EP2446027A1 PROTEIN 审中-公开
标题翻译：蛋白质
公开(公告)号：EP2446027A1
公开(公告)日：2012-05-02
申请号：EP10730253.1
申请日：2010-06-23
申请人： Danisco A/S
发明人： MIASNIKOV, Andrei , BOTT, Richard, R , SØRENSEN, Jens, Frisbæk
IPC分类号： C12N9/18 , C12N9/20 , A23L1/015 , C12N15/55
CPC分类号： C12N9/20 , A21D8/042 , C12N9/18 , C12Y301/01003 , C12Y301/01004 , C12Y301/01026 , C12Y301/01032
摘要： The present invention relates to a method for preparing a variant lipolytic enzyme comprising expressing in a host organism a nucleotide sequence which has at least 90% identity with a nucleotide sequence encoding a fungal lipolytic enzyme and comprises at at least one modification at a position which corresponds in the encoded amino acid sequence to a) the introduction of at least one glycosylation site (or one additional glycosylation site) in the amino acid sequence compared with the original fungal lipolytic enzyme; b) the introduction of at least one amino acid at a surface position on the polypeptide and at a location in an external loop distal to the active site (catalytic triad) of the enzyme which is more hydrophilic (compared with the original amino acid); or c) a substitution or insertion at one or more of positions 33, 63, 78, 190, 305, 306 or 320 or a deletion at one or more positions 311-312 or 307-319, wherein each amino acid position corresponds to the position of the amino acid sequence when aligned with SEQ ID No. 2; wherein when the nucleotide sequence has at least 90% identity with a nucleotide sequence encoding the fungal lipolytic enzyme shown in SEQ ID No. 22 or SEQ ID No. 23 the modification is not a substitution at position 63 and the deletion is not at position 311 -312. Preferably the nucleotide sequence has at least 90% identity with SEQ ID No. 1. The invention also relates to polypeptide produced by the method and to novel nucleic acids.

10. 发明公开

EP2382311A1 POLYPEPTIDES WITH XYLANASE ACTIVITY 无效转让
标题翻译：木聚糖酶多肽
公开(公告)号：EP2382311A1
公开(公告)日：2011-11-02
申请号：EP09834137.3
申请日：2009-12-23
申请人： Danisco A/S
发明人： SIBBESEN, Ole , SØRENSEN, Jens, Frisbæk
IPC分类号： C12N9/24
CPC分类号： C12N9/248
摘要： Polypeptides with xylanase activity modified to increase bran solubilisation and/or xylanase activity. The modification comprises modification of one or more amino acids in position 113, 122 or 175 in combination with one or more further amino acid modifications in position 11, 12, 13, 34, 54, 77, 81, 82, 104, 110, 113, 118, 122, 141, 154, 159, 162, 164, 166, 175 or 179, wherein the positions are determined as the position corresponding to the position of Bacillus subtilis xylanase (SEQ ID NO 1).

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