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1. 发明专利

JP2011153152A Method for purifying protein 审中-公开
标题翻译：净化蛋白质的方法
公开(公告)号：JP2011153152A
公开(公告)日：2011-08-11
申请号：JP2011076689
申请日：2011-03-30
申请人： Danisco As , ダニスコエイ/エス
发明人： JOHANSEN CLAUS LINDVALD , KJAERULFF SOEREN , MADRID SUSAN MAMPUSTI , PEDERSEN HENRIK , POULSEN CHARLOTTE HORSMANS , ZARGAHI MASOUD RAJABI
IPC分类号： C07K1/14 , C12N15/09 , C07K14/00 , C07K14/39 , C07K14/47 , C12N1/15 , C12N1/19 , C12N1/21 , C12N9/04 , C12N15/53 , C12N15/81 , C12P21/00 , C12P21/02 , C12Q1/02 , C12Q1/68
CPC分类号： C12P21/02 , C07K14/39 , C12N15/81
摘要： PROBLEM TO BE SOLVED: To provide a method for purifying a protein. SOLUTION: A method for releasing a soluble or membrane-associated intracellular protein of interest (POI) is described. The method comprises the following steps: a step for providing a cell comprising a soluble or membrane-associated intracellular POI; a step for bringing the cell to contact with a membrane extracting composition; and a step for causing the POI to be released from the cell under conditions sufficient for the specific release of the POI and in a soluble form. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：待解决的问题：提供蛋白质的纯化方法。解决方案：描述了释放可溶性或膜相关细胞内感兴趣的蛋白质（POI）的方法。该方法包括以下步骤：提供包含可溶性或膜相关细胞内POI的细胞的步骤; 使细胞与膜提取组合物接触的步骤; 以及在足以特异性释放POI和可溶形式的条件下使POI从细胞释放的步骤。版权所有（C）2011，JPO＆INPIT

2. 发明申请

WO2005087918A9 FUNGAL LIPOLYTIC ENZYMES 审中-公开
标题翻译：真菌脂肪酶
公开(公告)号：WO2005087918A9
公开(公告)日：2006-08-24
申请号：PCT/IB2005000875
申请日：2005-03-10
申请人： DANISCO , BRUNSTEDT JANNE , MIKKELSEN JOERN DALGAARD , PEDERSEN HENRIK , SOEE JOERN BORCH
发明人： BRUNSTEDT JANNE , MIKKELSEN JOERN DALGAARD , PEDERSEN HENRIK , SOEE JOERN BORCH
IPC分类号： C12N9/00 , A21D8/04 , A23C19/032 , A23K1/165 , A23K1/18 , A23L1/03 , A23L1/105 , A23L1/16 , A23L1/24 , C11B3/00 , C12N9/18
CPC分类号： A21D8/042 , A23C19/0328 , A23D7/02 , A23K20/189 , A23K50/75 , A23L7/107 , A23L7/109 , A23L7/111 , A23L27/60 , A23L29/06 , C11B3/003 , C12N9/18 , C12P7/62 , C12P19/44 , C12Y301/01
摘要： A fungal wild-type lipolytic enzyme having a higher ratio of activity on polar lipids compared with triglycerides, wherein the enzyme preferably has a phospholipidAriglyceride hydrolysing activity ratio of at least 4. Preferably, the lipolytic enzyme according to the present invention has a glycolipid:triglyceride hydrolysing activity ratio of at least 1.5. In one embodiment, the fungal lipolytic enzyme according to the present invention comprises an amino acid sequence as shown in SEQ ID No. 1 or SEQ ID No. 2 or SEQ ID No. 4 or SEQ ID No. 6 or an amino acid sequence which has at least 90% identity thereto. The present invention further encompasses a nucleic acid encoding a fungal lipolytic enzyme, which nucleic acid is selected from the group consisting of: (a) a nucleic acid comprising a nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7; (b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7 by the degeneration of the genetic code; and (c) nucleic acid comprising a nucleotide sequence which has at least 90% identity with the nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7.
摘要翻译：与甘油三酸酯相比，极性脂质的活性比例更高的真菌野生型脂肪分解酶，其中该酶优选具有至少4个磷脂酸甘油酯水解活性比。优选地，根据本发明的脂肪分解酶具有糖脂：甘油三酯水解活性比至少为1.5。在一个实施方案中，根据本发明的真菌脂肪分解酶包含如SEQ ID No.1或SEQ ID No.2或SEQ ID No.4或SEQ ID No.6所示的氨基酸序列或氨基酸序列，与其具有至少90％的同一性。本发明还包括编码真菌脂肪分解酶的核酸，该核酸选自：（a）包含SEQ ID No.3，SEQ ID No.5或SEQ SEQ ID No.5所示核苷酸序列的核酸第7号; （b）通过遗传密码的退化与SEQ ID No.3，SEQ ID No.5或SEQ ID No.7的核苷酸序列有关的核酸; 和（c）核酸，其包含与SEQ ID No.3，SEQ ID No.5或SEQ ID No.7所示的核苷酸序列具有至少90％同一性的核苷酸序列。

3. 发明申请

WO2004029084A3 LIPASE INHIBITORS AND USES THEREOF 审中-公开
标题翻译：脂肪酶抑制剂及其用途
公开(公告)号：WO2004029084A3
公开(公告)日：2004-11-25
申请号：PCT/IB0304585
申请日：2003-09-26
申请人： DANISCO , PEDERSEN HENRIK , POULSEN CHARLOTTE HORSMANS , SOEE JOERN BORCH , ZARGAHI MASOUD RAJABI
发明人： PEDERSEN HENRIK , POULSEN CHARLOTTE HORSMANS , SOEE JOERN BORCH , ZARGAHI MASOUD RAJABI
IPC分类号： A21D8/04 , A23L7/104 , C07K14/415 , C11D3/384 , C11D3/386 , C12N9/00 , C12N9/18 , C12N9/20 , C12N15/55 , C12Q1/44
CPC分类号： A21D8/042 , C07K14/415 , C11D3/384 , C11D3/38627 , C12N9/18 , C12N9/20 , C12Q1/44 , G01N2500/04
摘要： The present invention relates to the isolation of and characterization of a novel lipase inhibitor and its effect on different lipases. The present invention also relates to the use of a lipase inhibitor as a screen for lipases. The present invention also relates to the use of the inhibitor and/or lipases identified by a lipase inhibitor in food and/or feed technologies.
摘要翻译：本发明涉及新型脂肪酶抑制剂的分离和表征及其对不同脂肪酶的作用。本发明还涉及脂肪酶抑制剂作为脂肪酶筛选的用途。本发明还涉及在食品和/或饲料技术中由脂肪酶抑制剂鉴定的抑制剂和/或脂肪酶的用途。

4. 发明申请

WO2005087918A2 PROTEIN 审中-公开
标题翻译：蛋白
公开(公告)号：WO2005087918A2
公开(公告)日：2005-09-22
申请号：PCT/IB2005000875
申请日：2005-03-10
申请人： DANISCO , BRUNSTEDT JANNE , MIKKELSEN JOERN DALGAARD , PEDERSEN HENRIK , SOEE JOERN BORCH
发明人： BRUNSTEDT JANNE , MIKKELSEN JOERN DALGAARD , PEDERSEN HENRIK , SOEE JOERN BORCH
IPC分类号： A21D8/04 , A23C19/032 , A23K1/165 , A23K1/18 , A23L1/03 , A23L1/105 , A23L1/16 , A23L1/24 , C11B3/00 , C12N9/00 , C12N9/18
CPC分类号： A21D8/042 , A23C19/0328 , A23D7/02 , A23K20/189 , A23K50/75 , A23L7/107 , A23L7/109 , A23L7/111 , A23L27/60 , A23L29/06 , C11B3/003 , C12N9/18 , C12P7/62 , C12P19/44 , C12Y301/01
摘要： A fungal wild-type lipolytic enzyme having a higher ratio of activity on polar lipids compared with triglycerides, wherein the enzyme preferably has a phospholipidAriglyceride hydrolysing activity ratio of at least 4. Preferably, the lipolytic enzyme according to the present invention has a glycolipid:triglyceride hydrolysing activity ratio of at least 1.5. In one embodiment, the fungal lipolytic enzyme according to the present invention comprises an amino acid sequence as shown in SEQ ID No. 1 or SEQ ID No. 2 or SEQ ID No. 4 or SEQ ID No. 6 or an amino acid sequence which has at least 90% identity thereto. The present invention further encompasses a nucleic acid encoding a fungal lipolytic enzyme, which nucleic acid is selected from the group consisting of: (a) a nucleic acid comprising a nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7; (b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7 by the degeneration of the genetic code; and (c) nucleic acid comprising a nucleotide sequence which has at least 90% identity with the nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7.
摘要翻译：与甘油三酸酯相比，极性脂质的活性比例更高的真菌野生型脂肪分解酶，其中该酶优选具有至少4个磷脂酸甘油酯水解活性比。优选地，根据本发明的脂肪分解酶具有糖脂：甘油三酯水解活性比至少为1.5。在一个实施方案中，根据本发明的真菌脂肪分解酶包含如SEQ ID No.1或SEQ ID No.2或SEQ ID No.4或SEQ ID No.6所示的氨基酸序列或氨基酸序列，与其具有至少90％的同一性。本发明还包括编码真菌脂肪分解酶的核酸，该核酸选自：（a）包含SEQ ID No.3，SEQ ID No.5或SEQ SEQ ID No.5所示核苷酸序列的核酸第7号; （b）通过遗传密码的退化与SEQ ID No.3，SEQ ID No.5或SEQ ID No.7的核苷酸序列有关的核酸; 和（c）核酸，其包含与SEQ ID No.3，SEQ ID No.5或SEQ ID No.7所示的核苷酸序列具有至少90％同一性的核苷酸序列。

5. 发明专利

PT1722636E ENZIMAS LIPOLÍTICAS FÚNGICAS 未知
公开(公告)号：PT1722636E
公开(公告)日：2010-11-29
申请号：PT05718349
申请日：2005-03-10
申请人： DANISCO
发明人： SOE JORN BORCH , BRUNSTEDT JANNE , MIKKELSEN JOERN DALGAARD , PEDERSEN HENRIK
IPC分类号： A23C19/032 , A21D8/04 , A23K1/165 , A23K1/18 , A23L7/104 , A23L7/109 , A23L15/10 , A23L19/00 , A23L23/00 , A23L27/00 , A23L27/60 , A23L29/00 , C11B3/00 , C12N9/00 , C12N9/18 , C12N9/20

6. 发明专利

DE602005023838D1 PILZLICHE LIPOLYTISCHE ENZYME 未知
公开(公告)号：DE602005023838D1
公开(公告)日：2010-11-11
申请号：DE602005023838
申请日：2005-03-10
申请人： DANISCO
发明人： BRUNSTEDT JANNE , MIKKELSEN JOERN DALGAARD , PEDERSEN HENRIK , SOEE JOERN BORCH
IPC分类号： A21D8/04 , A23C19/032 , A23K1/165 , A23K1/18 , A23L7/104 , A23L7/109 , A23L15/10 , A23L19/00 , A23L23/00 , A23L27/00 , A23L27/60 , A23L29/00 , C11B3/00 , C12N9/00 , C12N9/18 , C12N9/20

7. 发明专利

AU2005221890B2 Protein 未知
公开(公告)号：AU2005221890B2
公开(公告)日：2010-07-22
申请号：AU2005221890
申请日：2005-03-10
申请人： DANISCO
发明人： PEDERSEN HENRIK , MIKKELSEN JORN DALGAARD , BRUNSTEDT JANNE , SOE JORN BORCH
IPC分类号： A21D8/04 , A23C19/032 , A23K1/165 , A23K1/18 , A23L7/104 , A23L7/109 , A23L15/10 , A23L19/00 , A23L23/00 , A23L27/00 , A23L27/60 , A23L29/00 , C11B3/00 , C12N9/00 , C12N9/18
摘要： A fungal wild-type lipolytic enzyme having a higher ratio of activity on polar lipids compared with triglycerides, wherein the enzyme preferably has a phospholipid:triglyceride hydrolysing activity ratio of at least 4. Preferably, the lipolytic enzyme according to the present invention has a glycolipid:triglyceride hydrolysing activity ratio of at least 1.5. In one embodiment, the fungal lipolytic enzyme according to the present invention comprises an amino acid sequence as shown in SEQ ID No. 1 or SEQ ID No. 2 or SEQ ID No. 4 or SEQ ID No. 6 or an amino acid sequence which has at least 90% identity thereto. The present invention further encompasses a nucleic acid encoding a fungal lipolytic enzyme, which nucleic acid is selected from the group consisting of: (a) a nucleic acid comprising a nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7; (b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7 by the degeneration of the genetic code; and (c) nucleic acid comprising a nucleotide sequence which has at least 90% identity with the nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7.

8. 发明专利

AU2005221890A2 Protein 未知
公开(公告)号：AU2005221890A2
公开(公告)日：2005-09-22
申请号：AU2005221890
申请日：2005-03-10
申请人： DANISCO
发明人： PEDERSEN HENRIK , MIKKELSEN JORN DALGAARD , BRUNSTEDT JANNE , SOE JORN BORCH
IPC分类号： C12N9/00 , A21D8/04 , A23C19/032 , A23K1/165 , A23K1/18 , A23L7/104 , A23L7/109 , A23L15/10 , A23L19/00 , A23L23/00 , A23L27/00 , A23L27/60 , A23L29/00 , C11B3/00 , C12N9/18
摘要： A fungal wild-type lipolytic enzyme having a higher ratio of activity on polar lipids compared with triglycerides, wherein the enzyme preferably has a phospholipid:triglyceride hydrolysing activity ratio of at least 4. Preferably, the lipolytic enzyme according to the present invention has a glycolipid:triglyceride hydrolysing activity ratio of at least 1.5. In one embodiment, the fungal lipolytic enzyme according to the present invention comprises an amino acid sequence as shown in SEQ ID No. 1 or SEQ ID No. 2 or SEQ ID No. 4 or SEQ ID No. 6 or an amino acid sequence which has at least 90% identity thereto. The present invention further encompasses a nucleic acid encoding a fungal lipolytic enzyme, which nucleic acid is selected from the group consisting of: (a) a nucleic acid comprising a nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7; (b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7 by the degeneration of the genetic code; and (c) nucleic acid comprising a nucleotide sequence which has at least 90% identity with the nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7.

9. 发明专利

PL356129A1 METHOD FOR PURIFYING PROTEINS 未知
公开(公告)号：PL356129A1
公开(公告)日：2004-06-14
申请号：PL35612900
申请日：2000-11-24
申请人： DANISCO
发明人： JOHANSEN CLAUS LINDVALD , KJAERULFF SOREN , MADRID SUSAN MAMPUSTI , PEDERSEN HENRIK , POULSEN CHARLOTTE HORSMANS , ZARGAHI MASOUS RAJABI
IPC分类号： C12N15/09 , C07K14/00 , C07K14/39 , C12N9/04 , C12N15/53 , C12N15/81 , C12P21/00 , C12P21/02 , C12Q1/02 , C12Q1/68 , C12N1/06

10. 发明专利

SI1722636T1 Fungalni lipolitični encimi 未知
公开(公告)号：SI1722636T1
公开(公告)日：2011-01-31
申请号：SI200531172
申请日：2005-03-10
申请人： DANISCO
发明人： BRUNSTEDT JANNE , MIKKELSEN JORN DALGAARD , PEDERSEN HENRIK , SOE JORN BORCH
IPC分类号： A21D8/04 , A21D8/00 , A23C19/032 , A23K1/165 , A23K1/18 , A23L7/104 , A23L7/109 , A23L15/10 , A23L19/00 , A23L23/00 , A23L27/00 , A23L27/60 , A23L29/00 , C11B3/00 , C12N9/00 , C12N9/18

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