会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 3. 发明申请
    • PROCESS
    • 处理
    • WO2009024862A3
    • 2009-05-22
    • PCT/IB2008002573
    • 2008-08-14
    • DANISCOLARSEN NEILS ERIKSOEE JOERN BORCH
    • LARSEN NEILS ERIKSOEE JOERN BORCH
    • A23C19/032A21D8/04C07K14/195C12N9/10C12P7/64C12Q1/48
    • A21D8/042A23C19/0328A23C19/0684A23L15/25A23L29/06C12N9/1029C12N9/18C12N11/00C12Y301/01
    • The use of a lipid acyltransferase in the manufacture of UHT milk for improving the stability, particularly the long term stability, and/or improving the perceptible sensory difference and/or improving smell and/or taste and/or for reducing the cholesterol content and/or for eliminating or reducing creaming of the UHT milk for reducing the cholesterol content in the UHT milk. A method of producing UHT milk, wherein method comprises admixing a lipid acyltransferase and milk (including a step of processing the milk to make it a UHT milk). Preferably said lipid acyltranferase is a polypeptide having lipid acyltransferase activity which polypeptide is obtained by expression of the nucleotide sequence shown as SEQ ID No. 49 or a nucleotide sequence which as has 70% or more identity therewith; and/or is obtained by expression of a nucleic acid which hybridises under medium stringency conditions to a nucleic probe comprising the nucleotide sequence shown as SEQ ID No. 49; and/or is a polypeptide having lipid acyltranferase activity which polypeptide comprises the amino acid sequence shown as SEQ ID No. 68 or an amino acid sequence sequence which as has 70% or more identity therewith.
    • 脂质酰基转移酶在制造UHT乳中用于改善稳定性,特别是长期稳定性,和/或改善可察觉的感觉差异和/或改善气味和/或味道和/或降低胆固醇含量和/ 或用于消除或减少超高温处理牛奶的分层以降低超高温灭菌牛奶中的胆固醇含量。 一种生产超高温灭菌乳的方法,其中方法包括混合脂质酰基转移酶和乳(包括处理乳以使其成为超高温灭菌乳的步骤)。 优选地,所述脂质酰基转移酶是具有脂质酰基转移酶活性的多肽,所述多肽通过表达SEQ ID No.49所示的核苷酸序列或与其具有70%或更多同一性的核苷酸序列获得; 和/或通过表达在中等严格条件下与包含如SEQ ID No.49所示核苷酸序列的核酸探针杂交的核酸获得; 和/或是具有脂质酰基转移酶活性的多肽,该多肽包含如SEQ ID No.68所示的氨基酸序列或与其具有70%或更多同一性的氨基酸序列序列。
    • 4. 发明申请
    • LIPOLYTIC ENZYME USES THEREOF IN THE FOOD INDUSTRY
    • 脂肪酶在食品工业中的应用
    • WO2006008653A3
    • 2007-03-29
    • PCT/IB2005002602
    • 2005-07-18
    • DANISCOMIASNIKOV ANDREISOEE JOERN BORCHMIKKELSEN JOERN DALGAARDPOVELAINEN MIRAPITKANEN VIRVE
    • MIASNIKOV ANDREISOEE JOERN BORCHMIKKELSEN JOERN DALGAARDPOVELAINEN MIRAPITKANEN VIRVE
    • C11B3/00A21D2/26A23C9/13A23L9/10A23L13/00A23L13/40A23L13/50A23L15/00A23L17/00A23L27/24A23L27/60A23L29/00C07F9/10C11C3/00C12N9/20C12N15/55C12P7/64
    • C12N9/1029A21D8/042A23J7/00C11B3/003C12N9/20C12P7/6418C12P7/6445C12P7/6481C12Y301/01003
    • The invention also encompases the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein said lipolytic enzyme is capable of hydrolysing a glycolipid or a phospholipid or transferring an acyl group from a glycolipid or phospholipids to a acyl acceptor. Preferably, the lipolytic enzyme for use in these methods and uses comprises an amino acid sequence as shown in any one of SEQ. ID. No.s 4, 5, 7, 8, 12, 14 or 16 or an amino acid sequence having at least 70% identity therewith or comprises a nucleotide sequence shown as SEQ ID No. 3, 6, 9, 13, 15 or 17 or a nulceotide seqeunce which has at least 70% identity therewith. The present invention also relates to a lipolytic enzyme capable of hydrolysing at least a galactolipid or capable of transferring an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Steptomyces species and includes a lipolytic enzyme comprising an amino acid sequence as shown in SEQ ID No. 4 or an amino acid sequence which has at least 60% identity thereto. The enzyme may be encoded by a nucleic acid selected from the group consisting of: a) a nucleic acid comprising a nucleotide sequence shown in SEQ ID No. 3; b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3 by the degeneration of the genetic code; and c) a nucleic acid comprising a nucleotide sequence which has at least 70% identity with the nucleotide sequence shown in SEQ ID No. 3.
    • 本发明还包括使用可从各种方法获得的脂肪分解酶:链霉菌,棒状杆菌和热嗜热菌,其中所述脂肪分解酶能够水解糖脂或磷脂或从糖脂转移酰基 或磷脂转化为酰基受体。 优选地,用于这些方法和用途的脂肪分解酶包含SEQ ID NO:1中任一项所示的氨基酸序列。 ID。 第4,5,7,8,12,14或16号氨基酸序列或与其具有至少70%同一性的氨基酸序列,或包含SEQ ID No.3,6,13,13,15或17所示的核苷酸序列 或与其具有至少70%同一性的nucceotide seqonunce。 本发明还涉及能够至少水解半乳糖脂或能够将酰基从半乳糖脂转移至一种或多种酰基受体底物的脂解酶,其中所述酶可从步态物种获得,并且包括脂肪分解酶,其包含氨基 如SEQ ID No.4所示的酸序列或与其具有至少60%同一性的氨基酸序列。 该酶可以由选自以下的核酸编码:a)包含SEQ ID No.3所示核苷酸序列的核酸; b)通过遗传密码的退化与SEQ ID No.3的核苷酸序列相关的核酸; 和c)包含与SEQ ID No.3所示核苷酸序列具有至少70%同一性的核苷酸序列的核酸。
    • 5. 发明申请
    • PROTEIN
    • 蛋白
    • WO2005087918A2
    • 2005-09-22
    • PCT/IB2005000875
    • 2005-03-10
    • DANISCOBRUNSTEDT JANNEMIKKELSEN JOERN DALGAARDPEDERSEN HENRIKSOEE JOERN BORCH
    • BRUNSTEDT JANNEMIKKELSEN JOERN DALGAARDPEDERSEN HENRIKSOEE JOERN BORCH
    • A21D8/04A23C19/032A23K1/165A23K1/18A23L1/03A23L1/105A23L1/16A23L1/24C11B3/00C12N9/00C12N9/18
    • A21D8/042A23C19/0328A23D7/02A23K20/189A23K50/75A23L7/107A23L7/109A23L7/111A23L27/60A23L29/06C11B3/003C12N9/18C12P7/62C12P19/44C12Y301/01
    • A fungal wild-type lipolytic enzyme having a higher ratio of activity on polar lipids compared with triglycerides, wherein the enzyme preferably has a phospholipidAriglyceride hydrolysing activity ratio of at least 4. Preferably, the lipolytic enzyme according to the present invention has a glycolipid:triglyceride hydrolysing activity ratio of at least 1.5. In one embodiment, the fungal lipolytic enzyme according to the present invention comprises an amino acid sequence as shown in SEQ ID No. 1 or SEQ ID No. 2 or SEQ ID No. 4 or SEQ ID No. 6 or an amino acid sequence which has at least 90% identity thereto. The present invention further encompasses a nucleic acid encoding a fungal lipolytic enzyme, which nucleic acid is selected from the group consisting of: (a) a nucleic acid comprising a nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7; (b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7 by the degeneration of the genetic code; and (c) nucleic acid comprising a nucleotide sequence which has at least 90% identity with the nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7.
    • 与甘油三酸酯相比,极性脂质的活性比例更高的真菌野生型脂肪分解酶,其中该酶优选具有至少4个磷脂酸甘油酯水解活性比。优选地,根据本发明的脂肪分解酶具有糖脂:甘油三酯 水解活性比至少为1.5。 在一个实施方案中,根据本发明的真菌脂肪分解酶包含如SEQ ID No.1或SEQ ID No.2或SEQ ID No.4或SEQ ID No.6所示的氨基酸序列或氨基酸序列, 与其具有至少90%的同一性。 本发明还包括编码真菌脂肪分解酶的核酸,该核酸选自:(a)包含SEQ ID No.3,SEQ ID No.5或SEQ SEQ ID No.5所示核苷酸序列的核酸 第7号; (b)通过遗传密码的退化与SEQ ID No.3,SEQ ID No.5或SEQ ID No.7的核苷酸序列有关的核酸; 和(c)核酸,其包含与SEQ ID No.3,SEQ ID No.5或SEQ ID No.7所示的核苷酸序列具有至少90%同一性的核苷酸序列。
    • 8. 发明申请
    • LIPOLYTIC ENZYME USES THEREOF IN THE FOOD INDUSTRY
    • 脂肪酶在食品工业中的应用
    • WO2006008653A9
    • 2007-02-22
    • PCT/IB2005002602
    • 2005-07-18
    • DANISCOMIASNIKOV ANDREISOEE JOERN BORCHMIKKELSEN JOERN DALGAARDPOVELAINEN MIRAPITKANEN VIRVE
    • MIASNIKOV ANDREISOEE JOERN BORCHMIKKELSEN JOERN DALGAARDPOVELAINEN MIRAPITKANEN VIRVE
    • C11B3/00A21D2/26A23C9/13A23L9/10A23L13/00A23L13/40A23L13/50A23L15/00A23L17/00A23L27/24A23L27/60A23L29/00C07F9/10C11C3/00C12N9/20C12N15/55C12P7/64
    • C12N9/1029A21D8/042A23J7/00C11B3/003C12N9/20C12P7/6418C12P7/6445C12P7/6481C12Y301/01003
    • The invention also encompases the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein said lipolytic enzyme is capable of hydrolysing a glycolipid or a phospholipid or transferring an acyl group from a glycolipid or phospholipids to a acyl acceptor. Preferably, the lipolytic enzyme for use in these methods and uses comprises an amino acid sequence as shown in any one of SEQ. ID. No.s 4, 5, 7, 8, 12, 14 or 16 or an amino acid sequence having at least 70% identity therewith or comprises a nucleotide sequence shown as SEQ ID No. 3, 6, 9, 13, 15 or 17 or a nulceotide seqeunce which has at least 70% identity therewith. The present invention also relates to a lipolytic enzyme capable of hydrolysing at least a galactolipid or capable of transferring an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Steptomyces species and includes a lipolytic enzyme comprising an amino acid sequence as shown in SEQ ID No. 4 or an amino acid sequence which has at least 60% identity thereto. The enzyme may be encoded by a nucleic acid selected from the group consisting of: a) a nucleic acid comprising a nucleotide sequence shown in SEQ ID No. 3; b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3 by the degeneration of the genetic code; and c) a nucleic acid comprising a nucleotide sequence which has at least 70% identity with the nucleotide sequence shown in SEQ ID No. 3.
    • 本发明还包括在各种方法和用途中可使用从下列属之一获得的脂解酶:链霉菌属,棒状杆菌属和嗜热菌属,其中所述脂解酶能够水解糖脂或磷脂或从糖脂转移酰基 或磷脂酰基受体。 优选地,用于这些方法和用途的脂解酶包含如SEQ.ID.NO.1中任一所示的氨基酸序列。 ID。 编号为4,5,7,8,12,14或16的氨基酸序列或与其具有至少70%同一性的氨基酸序列,或者包含如SEQ ID No.3,6,9,13,15或17所示的核苷酸序列 或与其具有至少70%同一性的核苷酸序列。 本发明还涉及能够水解至少半乳糖脂或能够将酰基从半乳糖脂转移到一个或多个酰基受体底物的脂肪分解酶,其中所述酶可以从链霉菌种获得并且包括含有氨基酸的脂解酶 如SEQ ID No.4所示的氨基酸序列或与其具有至少60%同一性的氨基酸序列。 该酶可以由选自以下的核酸编码:a)包含SEQ ID No.3所示的核苷酸序列的核酸; b)通过遗传密码的变性与核苷酸序列SEQ ID No.3相关的核酸; 和c)包含与SEQ ID No.3所示核苷酸序列具有至少70%同一性的核苷酸序列的核酸。