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    • 2. 发明申请
    • ELECTRONIC DETECTION OF INTERACTION AND DETECTION OF INTERACTION BASED ON THE INTERRUPTION FLOW
    • 相互作用的电子检测和基于中断流的相互作用检测
    • US20100124789A1
    • 2010-05-20
    • US12613420
    • 2009-11-05
    • Cynthia C. BAMDAD
    • Cynthia C. BAMDAD
    • G01N33/543G01N1/20G01N11/00
    • G01N33/54346B82Y30/00C12Q1/6816G01N33/54326G01N33/6803G01N2500/00Y10S436/806Y10T436/143333C12Q2565/629C12Q2563/155
    • Porous members can be positioned so as to partially or fully span channels in microfluidic systems. The porous members can be assembled and/or disassembled in situ. The porous members can be made such that pores are separated by connections including but a single molecule at one location, allowing for a high level of open area in a very small pore size member. The porous member can be made up of colloid particles interconnected with molecular species. These can be used to detect analytes qualitatively and/or quantitatively, or to selectively bind and/or release agents on command for a variety of purposes including first blocking, then opening a channel, concentrating analyte over time followed by release of analyte and detection downstream, etc. Porous members can define valves in multiple-channel systems and, with controlled binding and release of agents at the porous members, these valves can be opened and closed and fluid flow controlled in a multi-channel system. Fluidic systems of the invention can include multiple sensing locations at which different analytes are determined. Systems of the invention provide flexibility for overall microchemical analysis, sequentially, of a variety of agents.
    • 多孔构件可以定位成在微流体系统中部分地或完全跨过通道。 多孔构件可以原位组装和/或拆卸。 多孔构件可以制成使得孔通过包括但是在一个位置处的单个分子的连接分开,允许非常小的孔径构件中的高水平的开放面积。 多孔构件可以由与分子种类相互连接的胶体颗粒构成。 这些可用于定性和/或定量地检测分析物,或者为了各种目的选择性地结合和/或释放试剂,包括首先封闭,然后打开通道,随时间浓缩分析物,随后释放分析物和检测下游 多孔构件可以在多通道系统中限定阀,并且通过多孔构件处的试剂的受控结合和释放,可以打开和关闭这些阀,并且在多通道系统中控制流体流动。 本发明的流体系统可以包括确定不同分析物的多个感测位置。 本发明的系统为各种试剂的顺序的整体微量化学分析提供灵活性。