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1. 发明授权

US5010003A Use of yeast homologous signals to secrete heterologous proteins 失效
标题翻译：使用酵母同源信号分泌异源蛋白质
公开(公告)号：US5010003A
公开(公告)日：1991-04-23
申请号：US541186
申请日：1990-06-20
申请人： Chung N. Chang , Ronald A. Hitzeman , Mark D. Matteucci
发明人： Chung N. Chang , Ronald A. Hitzeman , Mark D. Matteucci
IPC分类号： C12N15/81
CPC分类号： C12N15/81
摘要： Described herein is the secretion from yeast of heterologous protein via a preprotein in which the heterologous protein is fused to the signal peptide of a yeast protein. The preprotein is processed by the cells to produce and secrete mature heterologous protein.
摘要翻译：本文描述的是异源蛋白质经酵母蛋白分泌，其中异源蛋白质与酵母蛋白质的信号肽融合。该蛋白由细胞处理以产生和分泌成熟的异源蛋白质。

2. 发明授权

US06414127B1 Pyrimidine derivatives for labeled binding partners 有权
标题翻译：用于标记结合配偶体的嘧啶衍生物
公开(公告)号：US06414127B1
公开(公告)日：2002-07-02
申请号：US09400502
申请日：1999-09-21
申请人： Kuei-Ying Lin , Mark D. Matteucci
发明人： Kuei-Ying Lin , Mark D. Matteucci
IPC分类号： C07H1900
CPC分类号： C07H19/06 , C07H21/00 , C12Q1/6813 , C12Q2525/101 , Y02P20/55 , C12Q2563/107
摘要： Compounds having structure (1) wherein R1 is —H a protecting group, a linker or a binding partner; and R2 and R34 are as defined in the specification. The invention also provides intermediates and methods make the structure (1) compounds, as well as methods to use the compounds as labels in diagnostic assays and to enhance binding to complementary bases.
摘要翻译：具有结构（1）的化合物，其中R 1为-H，保护基，连接基或结合配偶体; R2和R34如说明书中所定义。本发明还提供中间体和方法制备结构（1）化合物，以及在诊断测定中使用化合物作为标记并增强与互补碱基的结合的方法。

3. 发明授权

US5763588A Pyrimidine derivatives for labeled binding partners 失效
标题翻译：用于标记结合配偶体的嘧啶衍生物
公开(公告)号：US5763588A
公开(公告)日：1998-06-09
申请号：US481719
申请日：1995-06-07
申请人： Mark D. Matteucci , Robert J. Jones
发明人： Mark D. Matteucci , Robert J. Jones
IPC分类号： C12N15/09 , A61K31/565 , A61K31/70 , A61K31/7042 , A61K31/7052 , C07D239/46 , C07D239/47 , C07D239/52 , C07D239/54 , C07D405/04 , C07D487/04 , C07D487/14 , C07D491/14 , C07D498/04 , C07D498/14 , C07F7/22 , C07F9/09 , C07H19/00 , C07H19/04 , C07H19/06 , C07H19/22 , C07H21/00 , C07H21/02 , C12P19/34 , C12Q1/68 , C07D239/70
CPC分类号： C07D239/47 , C07D239/54 , C07D405/04 , C07D487/04 , C07F7/2212 , C07H19/06 , C07H19/073 , C07H21/00 , C12Q1/6816 , C12Q1/686 , Y02P20/55
摘要： Compounds having particular interest as labels and various novel uses in diagnostics and therapeutics are provided which have structure (1) ##STR1## wherein R.sup.1 is a binding partner, a linker or H; a and b are 0 or 1, provided that the total of a and b is 0 or 1; A is N or C; X is S, O, --C(O)--, NH or NCH.sub.2 R.sup.6 ; Y is --C(O)--; Z is taken together with A to form an aryl or heteroaryl ring structure comprising 5 or 6 ring atoms wherein the heteroaryl ring comprises a single O ring heteroatom, a single N ring heteroatom, a single S ring heteroatom, a single O and a single N ring heteroatom separated by a carbon atom, a single S and a single N ring heteroatom separated by a carbon atom, 2 N ring heteroatoms separated by a carbon atom, or 3 N ring heteroatoms at least two of which are separated by a carbon atom, and wherein the aryl or heteroaryl ring carbon atoms are unsubstituted with other than H or at least 1 nonbridging ring carbon atom is substituted with R.sup.6 or .dbd.O; R.sup.6 is independently H, C.sub.1 -C.sub.6 alkyl, C.sub.2 -C.sub.6 alkenyl, C.sub.2 -C.sub.6 alkynyl, NO.sub.2, N(R.sup.3).sub.2, C.tbd.N or halo, or an R.sup.6 is taken together with an adjacent R.sup.6 to complete a ring containing 5 or 6 ring atoms;, R.sup.3 is a protecting group or H; and tautomers, solvates and salts thereof; and provided that where a is 0, b is 1, and R.sup.1 is ##STR2## in which D.sup.2 is independently hydroxyl, blocked hydroxyl, mono, di or triphosphate, or an oligodeoxyribonucleotide otherwise containing only the bases A, G, T and C; and D.sup.3 is H or OH; then Z is not unsubstituted phenyl. Also provided are novel intermediates and methods for the preparation and use of the structure (1) compounds.
摘要翻译：提供具有特别感兴趣的化合物作为标记物和诊断和治疗中的各种新用途，其具有结构（1） r H; a和b为0或1，条件是a和b的总数为0或1; A是N或C; X是S，O，-C（O） - ，NH或NCH 2 R 6; Y是-C（O） - ; Z与A一起形成包含5或6个环原子的芳基或杂芳基环结构，其中杂芳基环包含单个O环杂原子，单个N环杂原子，单个S环杂原子，单个O和单个N 由碳原子分隔的环状杂原子，由碳原子分隔的单个S和单个N环杂原子，由碳原子分隔的2个N个杂原子或至少两个被碳原子分隔的3个N个环杂原子，并且其中所述芳基或杂芳基环碳原子未被取代，除了H或至少1个非桥环碳原子被R6或= O取代; R6独立地为H，C1-C6烷基，C2-C6烯基，C2-C6炔基，NO2，N（R3）2，C 3BONDN或卤素，或R6与相邻的R6一起形成含有5 或6个环原子; R 3为保护基或H; 和其互变异构体，溶剂合物和盐类; 并且条件是当a为0时，b为1，R1为羟基，单，二或三磷酸封端，或仅含有碱基A，G，T和C的寡脱氧核糖核苷酸; D3为H或OH; 那么Z不是未取代的苯基。还提供了用于制备和使用结构（1）化合物的新型中间体和方法。

4. 发明授权

US5548076A Method of making oligonucleotides, oligonucleoside thiophosphates and oligonucleoside phosphotriesters using H-phosphonate intermediates 失效
标题翻译：使用H-膦酸酯中间体制备寡核苷酸，寡核苷酸硫代磷酸酯和寡核苷磷酸三酯的方法
公开(公告)号：US5548076A
公开(公告)日：1996-08-20
申请号：US106246
申请日：1993-08-13
申请人： Brian C. Froehler , Mark D. Matteucci
发明人： Brian C. Froehler , Mark D. Matteucci
IPC分类号： C07H1/00 , C07H21/00 , C07H21/02 , C07H21/04 , C12N15/00 , C12N15/09 , C12Q1/68
CPC分类号： C07H21/00
摘要： A method is provided for the high fidelity, rapid and economical in vitro synthesis of oligonucleotides. Nucleoside H-phosphonates are condensed in seriatim using a dehydrating agent to produce a poly (nucleoside H-phosphonate). The product is oxidized to yield the desired oligonucleotide. A novel reagent is provided for multiple nucleoside additions in single cycles.
摘要翻译：提供了用于寡核苷酸的高保真，快速和经济的体外合成的方法。使用脱水剂将核苷H-膦酸酯在seriatim中冷凝以产生聚（核苷H-膦酸酯）。将产物氧化产生所需的寡核苷酸。提供了一种新的试剂，用于单次循环中的多种核苷添加。

5. 发明授权

US07511125B2 Pyrimidine derivatives for labeled binding partners 失效
标题翻译：用于标记结合配偶体的嘧啶衍生物
公开(公告)号：US07511125B2
公开(公告)日：2009-03-31
申请号：US10921734
申请日：2004-08-19
申请人： Kuei-Ying Lin , Mark D. Matteucci
发明人： Kuei-Ying Lin , Mark D. Matteucci
IPC分类号： C07H19/00 , C07H21/00
CPC分类号： C07H19/06 , C07H21/00 , C12Q1/6813 , C12Q2525/101 , Y02P20/55 , C12Q2563/107
摘要： Compounds having structure (1) wherein R1 is —H a protecting group, a linker or a binding partner; and R2 and R34 are as defined in the specification. The invention also provides intermediates and methods make the structure (1) compounds, as well as methods to use the compounds as labels in diagnostic assays and to enhance binding to complementary bases.
摘要翻译：具有结构（1）的化合物，其中R 1为-H，保护基，连接基或结合配偶体; R2和R34如说明书中所定义。本发明还提供中间体和方法制备结构（1）化合物，以及在诊断测定中使用化合物作为标记并增强与互补碱基的结合的方法。

6. 再颁专利

USRE39324E1 Pyrimidine derivatives and oligonucleotides containing same 有权
标题翻译：嘧啶衍生物和含有其的寡核苷酸
公开(公告)号：USRE39324E1
公开(公告)日：2006-10-03
申请号：US10080074
申请日：2002-02-21
申请人： Kuei-Ying Lin , Mark D. Matteucci
发明人： Kuei-Ying Lin , Mark D. Matteucci
IPC分类号： C07H19/00 , C07H21/00
CPC分类号： C07H19/06 , C07H21/00 , C12Q1/6813 , C12Q2525/101 , Y02P20/55 , C12Q2563/107
摘要： Compounds having structure (1) wherein R1 is —H a protecting group, a linker or a binding partner; and R2 and R34 are as defined in the specification. The invention also provides intermediates and methods make the structure (1) compounds, as well as methods to use the compounds as labels in diagnostic assays and to enhance binding to complementary bases.
摘要翻译：具有结构（1）的化合物，其中R 1是-H是保护基，连接基或结合配偶体; R 2和R 34如说明书中所定义。本发明还提供中间体和方法制备结构（1）化合物，以及在诊断测定中使用化合物作为标记并增强与互补碱基的结合的方法。

7. 发明授权

US5705693A Cationic lipids 失效
标题翻译：阳离子脂质
公开(公告)号：US5705693A
公开(公告)日：1998-01-06
申请号：US499699
申请日：1995-07-07
申请人： Randolph B. DePrince , Kevin L. Facchine , George S. Lewis , Jason G. Lewis , Kuei-Ying Lin , Mark D. Matteucci , Robert A. Mook, Jr. , Richard W. Wagner
发明人： Randolph B. DePrince , Kevin L. Facchine , George S. Lewis , Jason G. Lewis , Kuei-Ying Lin , Mark D. Matteucci , Robert A. Mook, Jr. , Richard W. Wagner
IPC分类号： A61K9/127 , A61K38/00 , C07C237/06 , C07C237/20 , C07C237/22 , C07C271/22 , C07C279/14 , C07C279/22 , C07C279/24 , C07J41/00 , C07K5/068 , C07K5/072 , C12N15/88
CPC分类号： C07K5/06095 , A61K9/1272 , C07C237/06 , C07C237/20 , C07C237/22 , C07C271/22 , C07C279/14 , C07C279/22 , C07C279/24 , C07J41/0055 , C07K5/06086 , C12N15/88 , A61K38/00
摘要： The present invention is directed to new cationic lipids and intermediates in their synthesis that are useful for transfecting nucleic acids or peptides into prokaryotic or eukaryotic cells. The lipids comprise one or two substituted arginine, lysine or ornithine residues, or derivatives thereof, linked to a lipophilic moiety. The lipids form a complex when mixed with polyanions such as nucleic acids or peptides. The complexes permit efficient transfer of polyanions into cells, usually without significant toxicity to the cells.
摘要翻译：本发明涉及其合成中的新的阳离子脂质和中间体，其可用于将核酸或肽转染到原核或真核细胞中。脂质包含与亲油部分连接的一个或两个取代的精氨酸，赖氨酸或鸟氨酸残基或其衍生物。当与聚阴离子例如核酸或肽混合时，脂质形成复合物。复合物允许聚阴离子有效转移到细胞中，通常对细胞没有显着的毒性。

8. 发明授权

US5646269A Method for oligonucleotide analog synthesis 失效
标题翻译：寡核苷酸类似物合成方法
公开(公告)号：US5646269A
公开(公告)日：1997-07-08
申请号：US234452
申请日：1994-04-28
申请人： Mark D. Matteucci , Jiancun Zhang
发明人： Mark D. Matteucci , Jiancun Zhang
IPC分类号： C07H19/04 , C07H19/10 , C07H19/20 , C07H21/00 , C07H19/06 , C07H19/16
CPC分类号： C07H19/10 , C07H19/04 , C07H19/20 , C07H21/00
摘要： The present invention is directed to improved methods to synthesize oligonucleotide analogs having an acetal linkage, such as a 3',5'-formacetal (3' --O--CH.sub.2 --O-- 5'), 3',5'-thioformacetal (3' --S--CH.sub.2 --O-- 5') or an analogous 2',5' linkage between adjacent nucleoside analog residues. Compositions comprising 5', 3' and 2' phosphinate nucleoside analogs useful in the methods are also provided.
摘要翻译：本发明涉及合成具有缩醛键的寡核苷酸类似物的改进方法，例如3'，5'-甲缩醛（3'-O-CH 2 -O-5'），3'，5'-硫代缩甲醛（3 '-S-CH2-O-5'）或相邻核苷类似物残基之间的类似的2'，5'键。还提供了包含用于该方法的5'，3'和2“次膦酸核苷类似物的组合物。

9. 发明授权

US5434257A Binding compentent oligomers containing unsaturated 3',5' and 2',5' linkages 失效
标题翻译：含有不饱和3'，5'和2'，5'键的结合性低聚物
公开(公告)号：US5434257A
公开(公告)日：1995-07-18
申请号：US142785
申请日：1993-10-26
申请人： Mark D. Matteucci , Xiaodong Cao
发明人： Mark D. Matteucci , Xiaodong Cao
IPC分类号： C07H19/06 , C07H21/00 , C12N15/113 , C12Q1/68 , C07H19/00
CPC分类号： C07H21/00 , C07H19/06 , C12N15/113 , C12Q1/68 , C12N2310/319
摘要： Oligonucleotide analogs having one or more substitute linkages of the formula 2'/3'--S--CH.sub.2 --CH.dbd.5' or 2'/3'--O--CH.sub.2 --CH.dbd.5' between adjacent nucleomonomers are disclosed. The substitute linkage replace the usual phosphodiester linkage found in unmodified nucleic acids. The oligonucleotide analogs are easy to synthesize, stable in vivo, resistant to endogenous nucleases and are able to hybridize to target nucleic acid sequences in a sequence specific manner.
摘要翻译：公开了具有相邻核单体之间的式2'/ 3'-S-CH2-CH = 5'或2'/ 3'-O-CH2-CH = 5'的一个或多个取代键的寡核苷酸类似物。替代连接取代未修饰核酸中常见的磷酸二酯连接。寡核苷酸类似物易于合成，体内稳定，对内源性核酸酶具有抗性，并且能够以序列特异性方式与靶核酸序列杂交。

10. 发明授权

US4959463A Intermediates 失效
标题翻译：中间体
公开(公告)号：US4959463A
公开(公告)日：1990-09-25
申请号：US910048
申请日：1986-09-19
申请人： Brian C. Froehler , Mark D. Matteucci
发明人： Brian C. Froehler , Mark D. Matteucci
IPC分类号： C07H1/00 , C07H21/00 , C07H21/02 , C07H21/04 , C12N15/00 , C12N15/09 , C12Q1/68
CPC分类号： C07H21/00
摘要： A method is provided for the high fidelity, rapid and economical in vitro synthesis of oligonucleotides. Nucleoside H-phosphonates are condensed in seriatim using a dehydrating agent to produce a poly (nucleoside H-phosphonate). The produce is oxidized to yield the desired oligonucleotide. A novel reagent is provided for multiple nucleoside additions in single cycles.
摘要翻译：提供了用于寡核苷酸的高保真，快速和经济的体外合成的方法。使用脱水剂将核苷H-膦酸酯在seriatim中冷凝以产生聚（核苷H-膦酸酯）。产物被氧化以产生所需的寡核苷酸。提供了一种新的试剂，用于单次循环中的多种核苷添加。

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