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1. 发明授权

US4012196A Colorimetric method for determining total lipids in human fluids 失效
标题翻译：用于测定人体液体中总脂质的比色法
公开(公告)号：US4012196A
公开(公告)日：1977-03-15
申请号：US578726
申请日：1975-05-19
申请人： Christopher S. Frings , Ted W. Fendley , Ralph T. Dunn
发明人： Christopher S. Frings , Ted W. Fendley , Ralph T. Dunn
IPC分类号： G01N33/92 , G01N33/16 , G01N21/02
CPC分类号： G01N33/92 , G01N2800/044
摘要： Phospho-vanillin reagent suitable for reaction with a mixture of sulfuric acid and an animal fluid containing at least 1 mg/ml lipids to determine total lipid concentration in the animal fluid comprising an aqueous solution containing vanillin and phosphoric acid having a vanillin to phosphoric acid mole ratio between 1.12 .times. 10.sup.-.sup.3 and 2.08 .times. 10.sup.-.sup.3. A sample of the animal fluid is mixed with concentrated sulfuric acid in a container, incubated, and the phospho-vanillin reagent is added to the mixture in the same container to form a chromogen. The light absorbance of the chromogen is measured and the total lipid concentration is determined in accordance with Beer's law from a standard curve relating total lipid concentration as a function of light absorbance by the chromogen.
摘要翻译：适用于与硫酸和含有至少1mg / ml脂质的动物流体的混合物反应的磷酸 - 香草醛试剂，以测定动物流体中的总脂质浓度，其包含含有香草醛和磷酸的水溶液，其含有香草醛与磷酸痣比例在1.12×10 -3和2.08×10 -3之间。将动物流体样品与浓硫酸在容器中混合，孵育，并将磷酸 - 香草醛试剂加入到同一容器中的混合物中以形成色原体。测量色原体的吸光度，并根据啤酒定律从总血脂浓度作为色原体的吸光度的函数的标准曲线测定总脂质浓度。

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