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1. 发明申请

US20150225764A1 METHOD FOR SIMULTANEOUSLY MEASURING ACTIVITY OF VARIOUS ENZYMES BY USING MULTI-WAVELENGTH ABSORPTION SINGLE CHANNEL 有权
标题翻译：通过使用多波长吸收单通道同时测量各种酶的活性的方法
公开(公告)号：US20150225764A1
公开(公告)日：2015-08-13
申请号：US14430391
申请日：2012-09-24
申请人： CHONGQING MEDICAL UNIVERSITY
发明人： Fei Liao , Xiaolan Yang , Hongbo Liu , Jizheng Dang , Gaobo Long , Yuanli Li , Lin Liu
IPC分类号： C12Q1/25 , G01N21/31
CPC分类号： C12Q1/25 , C12Q1/00 , C12Q2334/00 , G01N21/272 , G01N21/31 , G01N21/33 , G01N2021/3125 , G01N2333/904 , G01N2333/91074 , G01N2333/916 , G01N2333/938
摘要： A method for simultaneously measuring the activities of multiple kinds of enzymes in single channel through concimitantly monitoring multiple wavelength absorbances is claimed; based on linear additivity of absorbances and the isoabsorbance wavelengths of chromogenic substrates and their chromogenic products, both the principles for the combination of chromogenic substrates required for simultaneously measuring the activities of multiple kinds of enzymes in single channel through concimitantly monitoring multiple wavelength absorbances and an approach for data processing to eliminate the interference of the overlapped absorbances are developed, kinetic analysis of reaction curves via numerical integration eliminates the interference of all substrates and products and estimates the maximal reaction rates of the enzymes to be measured, giving the linear ranges and the limits of quantification for simultaneously measuring the activities of multiple kinds of enzymes in single channel comparable to those by separate assays; the present invention is applicable for simultaneously measuring the activities of multiple kinds of enzymes in biological samples, simultaneously measuring multiple components by enzyme-labeled immunoassays, screening simultaneously of inhibitors against multiple targets, for the practice in clinical biochemical analyses, clinical immunoassays, health laboratory analyses, the discovery of inhibitors, and the basical researches which need the present invention.
摘要翻译：要求一种同时测量单通道中多种酶活性的方法，通过对多波长吸收进行浓度监测; 基于发色底物及其显色产物的吸光度和异吸收波长的线性相加性，通过连续监测多波长吸光度同时测量单通道中多种酶的活性所需的显色底物的组合原理，以及方法为了消除重叠吸光度的干扰而进行数据处理，通过数值积分的反应曲线的动力学分析消除了所有底物和产物的干扰，并估计了待测酶的最大反应速率，给出了线性范围和极限用于同时测量单道中多种酶的活性的定量，与通过单独测定的酶相当; 本发明适用于同时测量生物样品中多种酶的活性，同时通过酶标免疫测定法测量多种成分，同时筛选多种靶标抑制剂，临床生化分析实践，临床免疫分析，健康实验室分析，抑制剂的发现以及需要本发明的基础研究。

2. 发明授权

US11209362B2 Fluorescent probe for detecting sulfenylated protein, preparation method thereof, and application thereof 有权
公开(公告)号：US11209362B2
公开(公告)日：2021-12-28
申请号：US16329941
申请日：2018-05-04
申请人： CHONGQING MEDICAL UNIVERSITY
发明人： Xiaolei Hu , Xiaolan Yang , Yibing Yin , Xiaomian Wu , Fei Liao , Jinpeng Lu , Juqiong Li
IPC分类号： G01N21/00 , G01N21/64 , C07C303/32 , C09K11/06
摘要： The present invention discloses a fluorescent probe for detecting a sulfenated protein, which has good stability and can specifically quantitatively detect a sulfenated protein in complex biological samples, and has a good detection ability of signal-to-noise ratios, is highly sensitive, and has excellent selectivity, thereby realizing specific detection of sulfhydryl sulfenation modification of intracellular proteins.

3. 发明授权

US09611502B2 Method for simultaneously measuring activity of various enzymes by using multi-wavelength absorption single channel 有权
公开(公告)号：US09611502B2
公开(公告)日：2017-04-04
申请号：US14430391
申请日：2012-09-24
申请人： Chongqing Medical University
发明人： Fei Liao , Xiaolan Yang , Hongbo Liu , Jizheng Dang , Gaobo Long , Yuanli Li , Lin Liu
IPC分类号： G01N21/31 , C12Q1/25 , G01N21/27 , C12Q1/00 , G01N21/33
CPC分类号： C12Q1/25 , C12Q1/00 , C12Q2334/00 , G01N21/272 , G01N21/31 , G01N21/33 , G01N2021/3125 , G01N2333/904 , G01N2333/91074 , G01N2333/916 , G01N2333/938
摘要： A method for simultaneously measuring the activities of multiple kinds of enzymes in single channel through concimitantly monitoring multiple wavelength absorbances is claimed; based on linear additivity of absorbances and the isoabsorbance wavelengths of chromogenic substrates and their chromogenic products, both the principles for the combination of chromogenic substrates required for simultaneously measuring the activities of multiple kinds of enzymes in single channel through concimitantly monitoring multiple wavelength absorbances and an approach for data processing to eliminate the interference of the overlapped absorbances are developed, kinetic analysis of reaction curves via numerical integration eliminates the interference of all substrates and products and estimates the maximal reaction rates of the enzymes to be measured, giving the linear ranges and the limits of quantification for simultaneously measuring the activities of multiple kinds of enzymes in single channel comparable to those by separate assays; the present invention is applicable for simultaneously measuring the activities of multiple kinds of enzymes in biological samples, simultaneously measuring multiple components by enzyme-labeled immunoassays, screening simultaneously of inhibitors against multiple targets, for the practice in clinical biochemical analyses, clinical immunoassays, health laboratory analyses, the discovery of inhibitors, and the basical researches which need the present invention.

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