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1. 发明授权

CN102879343B 用多波长吸收单通道同步测量多种酶活性的方法有权转让
公开(公告)号：CN102879343B
公开(公告)日：2014-10-29
申请号：CN201210355606.8
申请日：2012-09-21
申请人：重庆医科大学
发明人：廖飞 , 杨晓兰 , 刘红博 , 党济政 , 龙高波 , 李元丽 , 刘霖
IPC分类号： G01N21/31 , G01N21/33 , G01N21/35
CPC分类号： C12Q1/25 , C12Q1/00 , C12Q2334/00 , G01N21/272 , G01N21/31 , G01N21/33 , G01N2021/3125 , G01N2333/904 , G01N2333/91074 , G01N2333/916 , G01N2333/938
摘要：本发明公开了一种用多波长吸收单通道同步测量多种酶活性的方法；基于光吸收线性加和性及酶显色底物与其对应显色产物的等吸收波长，建立多波长吸收同步测量单通道同步测定多种酶活性所需显色底物组合设计原理及消除吸收光谱重叠干扰的数据处理方法，并以数值积分的速度方程分析酶反应过程测定最大反应速度消除各种底物及产物对待测酶活性的干扰，使单通道同步测定多种酶活性的范围及检测限都与单独测定时相当；该方法可用于同步测定生物样品多种酶活性、酶标记免疫分析同步测定多组分含量、同步筛选不同靶酶的抑制剂，可用于临床生化分析和临床免疫检验、卫生检验、酶抑制剂筛选等常规实践，也可用于这类酶法分析技术适用的各种基础研究。

2. 发明公开

CN102879343A 用多波长吸收单通道同步测量多种酶活性的方法有权转让
公开(公告)号：CN102879343A
公开(公告)日：2013-01-16
申请号：CN201210355606.8
申请日：2012-09-21
申请人：重庆医科大学
发明人：廖飞 , 杨晓兰 , 刘红博 , 党济政 , 龙高波 , 李元丽 , 刘霖
IPC分类号： G01N21/31 , G01N21/33 , G01N21/35
CPC分类号： C12Q1/25 , C12Q1/00 , C12Q2334/00 , G01N21/272 , G01N21/31 , G01N21/33 , G01N2021/3125 , G01N2333/904 , G01N2333/91074 , G01N2333/916 , G01N2333/938
摘要：本发明公开了一种用多波长吸收单通道同步测量多种酶活性的方法；基于光吸收线性加和性及酶显色底物与其对应显色产物的等吸收波长，建立多波长吸收同步测量单通道同步测定多种酶活性所需显色底物组合设计原理及消除吸收光谱重叠干扰的数据处理方法，并以数值积分的速度方程分析酶反应过程测定最大反应速度消除各种底物及产物对待测酶活性的干扰，使单通道同步测定多种酶活性的范围及检测限都与单独测定时相当；该方法可用于同步测定生物样品多种酶活性、酶标记免疫分析同步测定多组分含量、同步筛选不同靶酶的抑制剂，可用于临床生化分析和临床免疫检验、卫生检验、酶抑制剂筛选等常规实践，也可用于这类酶法分析技术适用的各种基础研究。

3. 发明申请

US20150225764A1 METHOD FOR SIMULTANEOUSLY MEASURING ACTIVITY OF VARIOUS ENZYMES BY USING MULTI-WAVELENGTH ABSORPTION SINGLE CHANNEL 有权
标题翻译：通过使用多波长吸收单通道同时测量各种酶的活性的方法
公开(公告)号：US20150225764A1
公开(公告)日：2015-08-13
申请号：US14430391
申请日：2012-09-24
申请人： CHONGQING MEDICAL UNIVERSITY
发明人： Fei Liao , Xiaolan Yang , Hongbo Liu , Jizheng Dang , Gaobo Long , Yuanli Li , Lin Liu
IPC分类号： C12Q1/25 , G01N21/31
CPC分类号： C12Q1/25 , C12Q1/00 , C12Q2334/00 , G01N21/272 , G01N21/31 , G01N21/33 , G01N2021/3125 , G01N2333/904 , G01N2333/91074 , G01N2333/916 , G01N2333/938
摘要： A method for simultaneously measuring the activities of multiple kinds of enzymes in single channel through concimitantly monitoring multiple wavelength absorbances is claimed; based on linear additivity of absorbances and the isoabsorbance wavelengths of chromogenic substrates and their chromogenic products, both the principles for the combination of chromogenic substrates required for simultaneously measuring the activities of multiple kinds of enzymes in single channel through concimitantly monitoring multiple wavelength absorbances and an approach for data processing to eliminate the interference of the overlapped absorbances are developed, kinetic analysis of reaction curves via numerical integration eliminates the interference of all substrates and products and estimates the maximal reaction rates of the enzymes to be measured, giving the linear ranges and the limits of quantification for simultaneously measuring the activities of multiple kinds of enzymes in single channel comparable to those by separate assays; the present invention is applicable for simultaneously measuring the activities of multiple kinds of enzymes in biological samples, simultaneously measuring multiple components by enzyme-labeled immunoassays, screening simultaneously of inhibitors against multiple targets, for the practice in clinical biochemical analyses, clinical immunoassays, health laboratory analyses, the discovery of inhibitors, and the basical researches which need the present invention.
摘要翻译：要求一种同时测量单通道中多种酶活性的方法，通过对多波长吸收进行浓度监测; 基于发色底物及其显色产物的吸光度和异吸收波长的线性相加性，通过连续监测多波长吸光度同时测量单通道中多种酶的活性所需的显色底物的组合原理，以及方法为了消除重叠吸光度的干扰而进行数据处理，通过数值积分的反应曲线的动力学分析消除了所有底物和产物的干扰，并估计了待测酶的最大反应速率，给出了线性范围和极限用于同时测量单道中多种酶的活性的定量，与通过单独测定的酶相当; 本发明适用于同时测量生物样品中多种酶的活性，同时通过酶标免疫测定法测量多种成分，同时筛选多种靶标抑制剂，临床生化分析实践，临床免疫分析，健康实验室分析，抑制剂的发现以及需要本发明的基础研究。

4. 发明申请

US20130109034A1 Compositions and Methods for Determining Celiac Disease 审中-公开
标题翻译：用于确定腹腔疾病的组合物和方法
公开(公告)号：US20130109034A1
公开(公告)日：2013-05-02
申请号：US13583125
申请日：2011-03-10
申请人： Vijay Kumar , Kishore S. Malyavantham
发明人： Vijay Kumar , Kishore S. Malyavantham
IPC分类号： G01N33/68 , C12N9/96
CPC分类号： G01N33/6854 , C12N9/96 , C12N15/62 , G01N2333/91074 , G01N2800/065
摘要： Provided are compositions and methods for diagnosis of celiac disease. The compositions include recombinant proteins that contain tissue transglutaminase and deamidated gliadin sequences. The gliadin sequences are repeated in the recombinant proteins. Also provided is a method for identifying an individual as having celiac disease based on the presence of antibodies in a sample from the individual, where the anti-bodies specifically recognize the recombinant protein, and identifying the individual as not having celiac disease based on an absence of antibodies that specifically recognize the recombinant protein.
摘要翻译：提供用于诊断乳糜泻的组合物和方法。组合物包括含有组织转谷氨酰胺酶和脱酰基麦醇溶蛋白序列的重组蛋白质。在重组蛋白中重复麦醇溶蛋白序列。还提供了一种基于来自个体的样品中存在抗体的个体鉴定为具有乳糜泻的方法，其中抗体特异性识别重组蛋白，并且基于缺失鉴定个体不具有乳糜泻的特异性识别重组蛋白的抗体。

5. 发明授权

EP3264081B1 DRY CHEMICAL TEST STRIP WITH MULTIPLE LAYERS OF MEMBRANES BASED ON CONCENTRATION GRADIENT 有权
公开(公告)号：EP3264081B1
公开(公告)日：2018-08-01
申请号：EP15882969.7
申请日：2015-02-28
申请人： Zeng, Rongbin
发明人： Zeng, Rongbin
IPC分类号： G01N33/52
CPC分类号： G01N33/525 , G01N33/00 , G01N2333/90283 , G01N2333/904 , G01N2333/91074 , G01N2333/916
摘要： A dry chemical test strip with multiple layers of membranes based on concentration gradient, comprising a substrate (1), an indicator layer (2), a reagent layer (3) and a diffusion layer (5), which are arranged successively from bottom to top, and further comprises a concentration gradient layer (4), which is arranged on the upper or lower surface of the reagent layer (3). Wherein a first reagent is uniformly applied on the reagent layer (3), and a second reagent is applied on the concentration gradient layer (4). The concentration gradient ˆ‡ Á of the second reagent is 0 in the width direction of the test strip, and is a constant or a function of the variable of length in the length direction of the test strip, and a chromogenic reagent is uniformly applied on the indicator layer (2). The dry chemical test strip with multiple layers of membranes based on concentration gradient can enable a continuous stepless change in the concentration distribution of the reagent on the test strip, allow all the testing and analysis procedures to be directly carried out on the test strip, and enable a test result to be directly obtained on the spot without the help of an instruments.

6. 发明申请

WO98054576A1 AN IMPROVED METHOD FOR DIAGNOSING ALCOHOL ABUSE 审中-公开
标题翻译：一种用于诊断酒精滥用的改进方法
公开(公告)号：WO98054576A1
公开(公告)日：1998-12-03
申请号：PCT/SE1998/001016
申请日：1998-05-28
IPC分类号： G01N33/98 , G01N33/68
CPC分类号： G01N33/98 , G01N2333/91074 , G01N2800/307
摘要： A method for diagnosing alcohol abuse of an individual is characterized in that the levels of CDT (marker 1) and at least one liver status marker (marker 2) are measured in a body fluid sample whereafter the levels for the markers are weighted to a common value according to a formula giving a better sensitivity and/or specificity than obtained for either or both of the markers, said value then being correlated to alcohol abuse.
摘要翻译：用于诊断个体的酒精滥用的方法的特征在于，在体液样品中测量CDT（标记物1）和至少一种肝脏状态标记物（标记物2）的水平，之后标记物的水平被加权到共同的根据给出比对于任一个或两个标记获得的更好的灵敏度和/或特异性的公式的值，所述值然后与酒精滥用相关。

7. 发明公开

EP3264081A4 DRY CHEMICAL TEST STRIP WITH MULTIPLE LAYERS OF MEMBRANES BASED ON CONCENTRATION GRADIENT 有权
公开(公告)号：EP3264081A4
公开(公告)日：2018-01-03
申请号：EP15882969
申请日：2015-02-28
申请人： ZENG RONGBIN
发明人： ZENG RONGBIN
IPC分类号： G01N33/52
CPC分类号： G01N33/525 , G01N33/00 , G01N2333/90283 , G01N2333/904 , G01N2333/91074 , G01N2333/916
摘要： A dry chemical test strip with multiple layers of membranes based on concentration gradient, comprising a substrate (1), an indicator layer (2), a reagent layer (3) and a diffusion layer (5), which are arranged successively from bottom to top, and further comprises a concentration gradient layer (4), which is arranged on the upper or lower surface of the reagent layer (3). Wherein a first reagent is uniformly applied on the reagent layer (3), and a second reagent is applied on the concentration gradient layer (4). The concentration gradient ˆ‡ Á of the second reagent is 0 in the width direction of the test strip, and is a constant or a function of the variable of length in the length direction of the test strip, and a chromogenic reagent is uniformly applied on the indicator layer (2). The dry chemical test strip with multiple layers of membranes based on concentration gradient can enable a continuous stepless change in the concentration distribution of the reagent on the test strip, allow all the testing and analysis procedures to be directly carried out on the test strip, and enable a test result to be directly obtained on the spot without the help of an instruments.

8. 发明公开

EP1913955A1 Novel method for treating H.pylori infections 审中-公开
标题翻译： Neue Methode zur Behandlung von Infektionen durch H. pylori
公开(公告)号：EP1913955A1
公开(公告)日：2008-04-23
申请号：EP06021936.7
申请日：2006-10-19
申请人： Gerhard, Markus
发明人： Schmees, Christian , Prinz, Christian, Lab 3K52, Room 20.01.12
IPC分类号： A61K39/106 , G01N33/50 , A61K38/00 , C12N9/10
CPC分类号： A61K39/105 , A61K39/00 , C07K16/121 , C07K16/40 , C12N9/104 , C12Q1/48 , C12Y203/02002 , G01N2333/91074 , Y02A50/472
摘要： Use of a ligand of the gamma-glutamyl transpeptldase of H. pylori (HPGGT) for the manufacture of a drug for the treatment of H. pylori infections, in particular gastro duodenal disorders, whereas the ligand significantly inhibits the HPGGT activity and abrogates the HPGGT dependent suppression of lymphocyte proliferation as assessed in a lymphocyte proliferation assay.
摘要翻译：使用幽门螺杆菌的γ-谷氨酰转肽酶（HPGGT）的配体用于制备用于治疗幽门螺杆菌感染的药物，特别是胃十二指肠疾病，而配体显着抑制HPGGT活性并废除HPGGT 在淋巴细胞增殖测定中评估淋巴细胞增殖的依赖性抑制。

9. 发明申请

US20100034846A1 NOVEL METHOD FOR TREATING H.PYLORI INFECTIONS 有权
标题翻译：用于治疗幽门螺杆菌感染的新方法
公开(公告)号：US20100034846A1
公开(公告)日：2010-02-11
申请号：US12446226
申请日：2007-10-19
申请人： Markus Gerhard , Christian Schmees
发明人： Markus Gerhard , Christian Schmees
IPC分类号： A61K39/02 , C07K14/00 , C07K7/08 , C07K16/12 , C12N15/11 , C12Q1/37 , A61K38/16
CPC分类号： A61K39/105 , A61K39/00 , C07K16/121 , C07K16/40 , C12N9/104 , C12Q1/48 , C12Y203/02002 , G01N2333/91074 , Y02A50/472
摘要： The present invention is related to a polypeptide comprising an amino acid sequence, whereby the amino acid sequence of the polypeptide is at least 80% identical to a stretch of consecutive amino acids of the region of HPGGT comprising an amino acid sequence corresponding to SEQ. ID. No. 1, whereby such region is defined by (a) amino acid positions 150 to 200 of the amino acid sequence according to SEQ. ID. No. 1, or (b) amino acid positions 410 to 480 of the amino acid sequence according to SEQ. ID. No. 1, and whereby the polypeptide is suitable to elicit an immune response which is capable of inhibiting the catalytic activity of HPGGT.
摘要翻译：本发明涉及包含氨基酸序列的多肽，其中多肽的氨基酸序列与包含对应于SEQ ID NO：1的氨基酸序列的HPGGT区域的连续氨基酸序列至少80％相同。 ID。其中这样的区域由（a）根据SEQ ID NO：1的氨基酸序列的150至200的氨基酸位置定义。 ID。或（b）根据SEQ ID NO：1的氨基酸序列的氨基酸位置410至480。 ID。该多肽适于引发能够抑制HPGGT的催化活性的免疫应答。

10. 发明申请

US20080206195A1 Compositions and Methods for Treating Disorders Associated with Abnormal Phosphate Metabolism 审中-公开
标题翻译：用于治疗与异常磷酸盐代谢相关的疾病的组合物和方法
公开(公告)号：US20080206195A1
公开(公告)日：2008-08-28
申请号：US11579998
申请日：2005-05-08
申请人： Eli Sprecher , Reuven Bergman
发明人： Eli Sprecher , Reuven Bergman
IPC分类号： A61K35/00 , A61K31/7088 , A61K38/43 , C12Q1/00 , C12Q1/68 , A61K38/02
CPC分类号： A61K38/45 , A61K48/00 , C12Q1/48 , G01N2333/91074 , G01N2500/04 , G01N2800/04 , G01N2800/10
摘要： The present invention uncovers that mutations in GALNT3 gene encoding UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T3) cause familial tumoral calcinosis (FTC). Methods and pharmaceutical compositions useful for treating disorders associated with abnormal phosphate metabolism are provided. Specifically, inducers of GalNAc-T3 can be used to treat hyperphosphatemia related disorders such as FTC, and on the other hand, inhibitors of GalNAc-T3 can be used to treat disorders associated with hypophosphatemia, such as hypophosphatemic rickets. The present invention further provides methods and kits for diagnosing familial tumoral calcinosis as well as for identifying agents suitable for treating disorders associated with abnormal phosphate metabolism.
摘要翻译：本发明揭示编码UDP-N-乙酰基-β-D-半乳糖胺的多肽N-乙酰半乳糖胺基转移酶（GalNAc-T3）的GALNT3基因中的突变引起家族性肿瘤性钙质沉积（FTC）。提供了可用于治疗与磷酸代谢异常有关的疾病的方法和药物组合物。具体来说，GalNAc-T3的诱导剂可用于治疗高磷酸盐血症相关疾病如FTC，另一方面，GalNAc-T3的抑制剂可用于治疗与低磷血症相关的疾病，如低磷血症性r r病。本发明还提供了用于诊断家族性肿瘤钙质沉积的方法和试剂盒，以及用于鉴定适合于治疗与磷酸代谢异常有关的疾病的药剂。

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