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1. 发明授权

US08309332B2 GRG23 and GRG51 polypeptides having herbicide resistance activity 有权
标题翻译：具有除草剂抗性活性的GRG23和GRG51多肽
公开(公告)号：US08309332B2
公开(公告)日：2012-11-13
申请号：US12468205
申请日：2009-05-19
申请人： Cheryl L. Peters , Jill Burdette Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
发明人： Cheryl L. Peters , Jill Burdette Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
IPC分类号： C12N9/00
CPC分类号： C12N15/8275 , C07K14/195 , C07K14/415 , C12N15/8274 , C12Q1/42
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO:1, 3, or 5. The present invention additionally provides a method to measure enzyme kinetic activity using fluorogenic substrates.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2,4或6所示的氨基酸序列的核苷酸序列或SEQ ID NO：1,3或5所示的核苷酸序列的分离的多核苷酸。本发明另外提供了使用荧光底物测量酶动力学活性的方法。

2. 发明授权

US07674958B2 GRG23 and GRG51 genes conferring herbicide resistance 有权
标题翻译：赋予除草剂抗性的GRG23和GRG51基因
公开(公告)号：US07674958B2
公开(公告)日：2010-03-09
申请号：US11605824
申请日：2006-11-29
申请人： Cheryl L. Peters , Jill Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
发明人： Cheryl L. Peters , Jill Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
IPC分类号： C12N15/82 , C12N15/63 , C12N15/52
CPC分类号： C12N15/8275 , C07K14/195 , C07K14/415 , C12N15/8274 , C12Q1/42
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO:1, 3, or 5. The present invention additionally provides a method to measure enzyme kinetic activity using fluorogenic substrates.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2,4或6所示的氨基酸序列的核苷酸序列或SEQ ID NO：1,3或5所示的核苷酸序列的分离的多核苷酸。本发明另外提供了使用荧光底物测量酶动力学活性的方法。

3. 发明申请

US20090227771A1 GRG23 AND GRG51 GENES CONFERRING HERBICIDE RESISTANCE 有权
标题翻译： GRG23和GRG51基因对抗药性的抵抗
公开(公告)号：US20090227771A1
公开(公告)日：2009-09-10
申请号：US12468205
申请日：2009-05-19
申请人： Cheryl L. Peters , Jill Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
发明人： Cheryl L. Peters , Jill Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
IPC分类号： C07K14/00
CPC分类号： C12N15/8275 , C07K14/195 , C07K14/415 , C12N15/8274 , C12Q1/42
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO:1, 3, or 5. The present invention additionally provides a method to measure enzyme kinetic activity using fluorogenic substrates.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2,4或6所示的氨基酸序列的核苷酸序列或SEQ ID NO：1,3或5所示的核苷酸序列的分离的多核苷酸。本发明另外提供了使用荧光底物测量酶动力学活性的方法。

4. 发明申请

US20100144529A1 IDENTIFICATION OF A NEW CLASS OF EPSP SYNTHASES 审中-公开
标题翻译：鉴定新类型的EPSP合成
公开(公告)号：US20100144529A1
公开(公告)日：2010-06-10
申请号：US12705874
申请日：2010-02-15
申请人： Nadine Carozzi , Brian Carr , Philip E. Hammer
发明人： Nadine Carozzi , Brian Carr , Philip E. Hammer
IPC分类号： A01N57/18 , C12N15/52 , C12N5/04 , A01H5/00 , A01H5/10 , A01H1/00 , A01P13/00
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring tolerance to glyphosate in bacteria, plants, plant cells, tissues and seeds are provided. Compositions include a novel class of EPSPS enzymes, designated Class III, and polynucleotides encoding such enzymes, vectors comprising those polynucleotides, and host cells comprising the vectors. The novel proteins comprise at least one sequence domain selected from the Class III domains provided herein. These sequence domains can be used to identify EPSP synthases with glyphosate resistance activity.
摘要翻译：提供了在细菌，植物，植物细胞，组织和种子中赋予草甘膦耐受性的组合物和方法。组合物包括命名为III类的新一类EPSPS酶和编码这些酶的多核苷酸，包含这些多核苷酸的载体和包含载体的宿主细胞。该新型蛋白质包含至少一个选自本文提供的III类结构域的序列结构域。这些序列结构域可用于鉴定具有草甘膦抗性活性的EPSP合成酶。

5. 发明授权

US07504561B2 GDC-1 genes conferring herbicide resistance 失效
标题翻译：赋予除草剂抗性的GDC-1基因
公开(公告)号：US07504561B2
公开(公告)日：2009-03-17
申请号：US11185342
申请日：2005-07-20
申请人： Philip E. Hammer , Todd K. Hinson , Brian Carr , Nicholas B. Duck
发明人： Philip E. Hammer , Todd K. Hinson , Brian Carr , Nicholas B. Duck
IPC分类号： C12N15/82 , C12N15/52
CPC分类号： C12N9/88 , C12N15/8275
摘要： Compositions and methods for conferring herbicide resistance to plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules corresponding to glyphosate resistant nucleic acid sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding an amino acid sequence shown in SEQ ID NO:3, 6, 8, 11, 19, or 21, or a nucleotide sequence set forth in SEQ ID NO:1, 2, 4, 5, 7, 9, 10, 18, or 20, as well as variants and fragments thereof.
摘要翻译：提供了赋予对植物，植物细胞，组织和种子的除草剂抗性的组合物和方法。提供了包含赋予对草甘膦除草剂的抗性或耐受性的多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。组合物还包括转化的植物，植物细胞，组织和种子。特别地，提供了对应于草甘膦抗性核酸序列的分离的核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3,6,8,11,19或21所示氨基酸序列的核苷酸序列或SEQ ID NO： 1，2，4，5，7，9，10，18或20，以及其变体和片段。

6. 发明申请

US20100144530A1 IDENTIFICATION OF A NEW CLASS OF EPSP SYNTHASES 审中-公开
公开(公告)号：US20100144530A1
公开(公告)日：2010-06-10
申请号：US12705891
申请日：2010-02-15
申请人： Nadine Carozzi , Brian Carr , Philip E. Hammer
发明人： Nadine Carozzi , Brian Carr , Philip E. Hammer
IPC分类号： A01N57/18 , C12N15/52 , A01H1/00 , C12N5/04 , A01H5/00 , A01H5/10 , A01P13/00
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring tolerance to glyphosate in bacteria, plants, plant cells, tissues and seeds are provided. Compositions include a novel class of EPSPS enzymes, designated Class III, and polynucleotides encoding such enzymes, vectors comprising those polynucleotides, and host cells comprising the vectors. The novel proteins comprise at least one sequence domain selected from the Class III domains provided herein. These sequence domains can be used to identify EPSP synthases with glyphosate resistance activity.

7. 发明申请

US20090151018A1 GDC-1 GENES CONFERRING HERBICIDE RESISTANCE 审中-公开
标题翻译： GDC-1基因抑制抗药性
公开(公告)号：US20090151018A1
公开(公告)日：2009-06-11
申请号：US12364724
申请日：2009-02-03
申请人： Philip E. Hammer , Todd K. Hinson , Brian Carr , Nicholas B. Duck
发明人： Philip E. Hammer , Todd K. Hinson , Brian Carr , Nicholas B. Duck
IPC分类号： C12N15/60 , A01H5/00 , C12N15/11 , C12N15/00 , C12N1/21 , C12N5/04 , C12N9/88 , C12P21/02
CPC分类号： C12N9/88 , C12N15/8275
摘要： Compositions and methods for conferring herbicide resistance to plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules corresponding to glyphosate resistant nucleic acid sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding an amino acid sequence shown in SEQ ID NO:3, 6, 8, 11, 19, or 21, or a nucleotide sequence set forth in SEQ ID NO:1, 2, 4, 5, 7, 9, 10, 18, or 20, as well as variants and fragments thereof.
摘要翻译：提供了赋予对植物，植物细胞，组织和种子的除草剂抗性的组合物和方法。提供了包含赋予对草甘膦除草剂的抗性或耐受性的多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。组合物还包括转化的植物，植物细胞，组织和种子。特别地，提供了对应于草甘膦抗性核酸序列的分离的核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3,6,8,11,19或21所示氨基酸序列的核苷酸序列或SEQ ID NO： 1，2，4，5，7，9，10，18或20，以及其变体和片段。

8. 发明授权

US08426204B2 Chlamydomonas EPSPS chloroplast transit peptide (CTP) and expression cassettes and transgenic plants utilizing the CTP 有权
标题翻译：衣原体EPSPS叶绿体转运肽（CTP）和表达盒和利用CTP的转基因植物
公开(公告)号：US08426204B2
公开(公告)日：2013-04-23
申请号：US12555175
申请日：2009-09-08
申请人： Philip E. Hammer , Vadim Beilinson , Todd K. Hinson
发明人： Philip E. Hammer , Vadim Beilinson , Todd K. Hinson
IPC分类号： A01H5/00 , C12N5/04 , C12N15/82
CPC分类号： C12N15/8279 , C12N15/8214 , C12N15/8274
摘要： The present invention provides compositions and methods for targeting polypeptides to the chloroplasts of higher plants. Compositions include expression cassettes having a nucleotide sequence encoding a chloroplast targeting peptide (CTP) operably linked to a nucleotide sequence of interest, wherein the CTP is derived from Chlamydomonas sp. Plant transformation vectors, plants and plant cells having the CTP sequences are also encompassed, as well as variants and fragments of the CTP sequences. Methods for expressing a heterologous nucleotide sequence in a plant using the CTP sequences disclosed herein are also provided.
摘要翻译：本发明提供了将多肽靶向高等植物的叶绿体的组合物和方法。组合物包括具有编码与感兴趣的核苷酸序列可操作地连接的叶绿体靶向肽（CTP）的核苷酸序列的表达盒，其中CTP衍生自衣藻属。还包括具有CTP序列的植物转化载体，植物和植物细胞，以及CTP序列的变体和片段。还提供了使用本文公开的CTP序列在植物中表达异源核苷酸序列的方法。

9. 发明授权

US07659376B2 Antibody that binds glyphosate resistance protein 有权
标题翻译：抗草甘膦抗性蛋白质的抗体
公开(公告)号：US07659376B2
公开(公告)日：2010-02-09
申请号：US12139408
申请日：2008-06-13
申请人： Philip E. Hammer , Nicholas B. Duck
发明人： Philip E. Hammer , Nicholas B. Duck
IPC分类号： C07K16/00
CPC分类号： C12N15/8275
摘要： Compositions and methods for conferring herbicide resistance to plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules encoding glyphosate resistance proteins are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or the nucleotide sequence set forth in SEQ ID NO:1.
摘要翻译：提供了赋予对植物，植物细胞，组织和种子的除草剂抗性的组合物和方法。提供了包含赋予对草甘膦除草剂的抗性或耐受性的多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。组合物还包括转化的植物，植物细胞，组织和种子。特别地，提供编码抗草甘膦蛋白质的分离的核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2所示的氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列的分离的核酸分子。

10. 发明申请

US20090241219A1 GDC-2 GENES CONFERRING HERBICIDE RESISTANCE 审中-公开
标题翻译： GDC-2基因对抗药性的抵抗
公开(公告)号：US20090241219A1
公开(公告)日：2009-09-24
申请号：US12423926
申请日：2009-04-15
申请人： Philip E. Hammer , Todd K. Hinson , Nicholas B. Duck
发明人： Philip E. Hammer , Todd K. Hinson , Nicholas B. Duck
IPC分类号： A01H5/00 , C12N15/82 , C07H21/04 , C12N15/63 , C12N1/21 , C12N5/10 , C12N9/14 , C12P21/02
CPC分类号： C12N9/88 , C12N15/8275 , C12N15/8278
摘要： Compositions and methods for conferring herbicide resistance to plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules corresponding to glyphosate resistant nucleic acid sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:3 or the nucleotide sequences set forth in SEQ ID NOS:1 and 2.
摘要翻译：提供了赋予对植物，植物细胞，组织和种子的除草剂抗性的组合物和方法。提供了包含赋予对草甘膦除草剂的抗性或耐受性的多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。组合物还包括转化的植物，植物细胞，组织和种子。特别地，提供了对应于草甘膦抗性核酸序列的分离的核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3所示的氨基酸序列的核苷酸序列或SEQ ID NO：1和2所示的核苷酸序列。

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