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1. 发明授权

US09255256B2 Expression of functional cytochorome P450 monooxygenase system in enterobacteria 有权
标题翻译：功能性细胞色素P450单加氧酶体系在肠细菌中的表达
公开(公告)号：US09255256B2
公开(公告)日：2016-02-09
申请号：US10369289
申请日：2003-02-18
申请人： Charles Roland Wolf , Thomas Herbert Friedberg , Michael Patrick Pritchard
发明人： Charles Roland Wolf , Thomas Herbert Friedberg , Michael Patrick Pritchard
IPC分类号： C12N15/53 , C12N9/02 , C12N15/52 , C12P1/00
CPC分类号： C12N9/0042 , C07K2319/02 , C12N9/0077 , C12N15/52 , C12P1/00
摘要： A bacterial cell containing a functional cytochrome P450 monooxygenase system, said cell comprising a genetic construct capable of expressing a cytochrome P450 and a genetic construct capable of expressing, separately from said cytochrome P450, a cytochrome P450 reductase wherein the N-terminus of the cytochrome P450 and the N-terminus of the cytochrome P450 reductase are each adapted to allow functional coupling of said cytochrome P450 and said cytochrome P450 reductase within said cell. A bacterial cell containing a cytochrome P450 comprising a genetic construct encoding, and capable of expressing, said cytochrome P450 wherein the cytochrome P450 comprises an N-terminal portion which directs the cytochrome P450 to a cellular compartment of membrane of the bacterial cell. The bacterial cells are useful as, for example, bioreactors, in drug testing and mutagenicity testing and as a source of cytochrome P450.
摘要翻译：一种含有功能性细胞色素P450单加氧酶系统的细菌细胞，所述细胞包含能够表达细胞色素P450的遗传构建体和能够与所述细胞色素P450分开表达细胞色素P450还原酶的遗传构建体，其中细胞色素P450的N末端并且细胞色素P450还原酶的N末端各自适于允许所述细胞内的所述细胞色素P450和所述细胞色素P450还原酶的功能偶联。含有细胞色素P450的细菌细胞，其包含编码并能够表达所述细胞色素P450的遗传构建体，其中细胞色素P450包含将细胞色素P450引导至细菌细胞膜的细胞区的N末端部分。细菌细胞可用作例如生物反应器，在药物测试和致突变性测试中以及作为细胞色素P450的来源。

2. 发明授权

US06566108B1 Expression of functional cytochrome P450 monooxygenase system in enterobacteria 有权
标题翻译：功能性细胞色素P450单加氧酶体系在肠细菌中的表达
公开(公告)号：US06566108B1
公开(公告)日：2003-05-20
申请号：US09230233
申请日：1999-05-17
申请人： Charles Roland Wolf , Thomas Herbert Friedberg , Michael Patrick Pritchard
发明人： Charles Roland Wolf , Thomas Herbert Friedberg , Michael Patrick Pritchard
IPC分类号： C12N902
CPC分类号： C12N9/0042 , A61K38/00 , C07K2319/02 , C12N9/0077
摘要： A bacterial cell containing a functional cytochrome P450 monooxygenase system, said cell comprising a genetic construct capable of expressing a cytochrome P450 and genetic construct capable of expressing, separately from said cytochrome P450, a cytochrome P450 reductase, wherein each of the cytochrome P450 and the cytochrome P450 reductase have at their N-terminus a bacterial signal peptide. The cytochrome P450 and the cytochrome P450 reductase may be encoded by different constructs or the same construct. A bacterial cell containing a cytochrome P450 comprising a genetic construct encoding, and capable of expressing, said cytochrome P450 wherein the cytochrome P450 has, at is N-terminal, a bacterial signal peptide. The bacterial cells are useful as, for example, bioreactors, in drug testing and mutagenicity testing and as a source of cytochrome P450.
摘要翻译：一种含有功能性细胞色素P450单加氧酶系统的细菌细胞，所述细胞包含能够表达细胞色素P450的遗传构建体和能够与所述细胞色素P450分开表达细胞色素P450还原酶的遗传构建体，其中细胞色素P450和细胞色素 P450还原酶在其N末端具有细菌信号肽。细胞色素P450和细胞色素P450还原酶可以由不同的构建体或相同的构建体编码。含有细胞色素P450的细菌细胞，其包含编码并能够表达所述细胞色素P450的遗传构建体，其中细胞色素P450具有细菌信号肽，为N末端。细菌细胞可用作例如生物反应器，在药物测试和致突变性测试中以及作为细胞色素P450的来源。

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