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1. 发明授权

US06287833B1 Method for detecting Candida infection 失效
标题翻译：检测念珠菌感染的方法
公开(公告)号：US06287833B1
公开(公告)日：2001-09-11
申请号：US08472599
申请日：1995-06-07
申请人： Charles Garrett Miyada , Arthur C. Switchenko , Melanie W. Quong , Man-Ying Laurie Wong
发明人： Charles Garrett Miyada , Arthur C. Switchenko , Melanie W. Quong , Man-Ying Laurie Wong
IPC分类号： C12N904
CPC分类号： C12N9/0006 , C12P19/02 , Y10S435/81 , Y10S435/921 , Y10S435/924
摘要： A D-arabinitol dehydrogenase enzyme is disclosed. The enzyme is capable of catalyzing the oxidation of D-arabinitol and substantially incapable of catalyzing the oxidation of D-mannitol and is substantially free of other enzymes capable of oxidizing D-mannitol. Also disclosed are methods for determining D-arabinitol. In one embodiment the method comprises the steps of providing in combination (1) a medium suspected of containing D-arabinitol and (2) a D-arabinitol dehydrogenase enzyme and examining the medium for the product of the oxidation of the D-arabinitol. The enzyme utilized is capable of catalyzing the oxidation of D-arabinitol and substantially incapable of catalyzing the oxidation of D-mannitol. Kits for conducting the present method are also disclosed.
摘要翻译：公开了D-阿拉伯糖醇脱氢酶。该酶能够催化D-阿拉伯糖醇的氧化，并且基本上不能催化D-甘露糖醇的氧化，并且基本上不含能够氧化D-甘露糖醇的其它酶。还公开了测定D-阿拉伯糖醇的方法。在一个实施方案中，该方法包括以下步骤：组合（1）怀疑含有D-阿拉伯糖醇的培养基和（2）D-阿拉伯糖醇脱氢酶，并检查培养基中D-阿拉伯糖醇的氧化产物。所使用的酶能够催化D-阿拉伯糖醇的氧化，并且基本上不能催化D-甘露糖醇的氧化。还公开了用于进行本方法的试剂盒。

2. 发明授权

US06280988B1 Method for detecting Candida infection 失效
公开(公告)号：US06280988B1
公开(公告)日：2001-08-28
申请号：US08487946
申请日：1995-06-07
申请人： Charles Garrett Miyada , Arthur C. Switchenko , Melanie W Quong , Man-Ying Laurie Wong
发明人： Charles Garrett Miyada , Arthur C. Switchenko , Melanie W Quong , Man-Ying Laurie Wong
IPC分类号： C12N904
CPC分类号： C12N9/0006 , C12P19/02 , Y10S435/81 , Y10S435/921 , Y10S435/924
摘要： A D-arabinitol dehydrogenase enzyme is disclosed. The enzyme is capable of catalyzing the oxidation of D-arabinitol and substantially incapable of catalyzing the oxidation of D-mannitol and is substantially free of other enzymes capable of oxidizing D-mannitol. Also disclosed are methods for determining D-arabinitol. In one embodiment the method comprises the steps of providing in combination (1) a medium suspected of containing D-arabinitol and (2) a D-arabinitol dehydrogenase enzyme and examining the medium for the product of the oxidation of the D-arabinitol. The enzyme utilized is capable of catalyzing the oxidation of D-arabinitol and substantially incapable of catalyzing the oxidation of D-mannitol. Kits for conducting the present method are also disclosed.

3. 发明授权

US5766874A Kit containing d-arabinitol dehydrogenase and NAD+ for determining d-arabinitol 失效
标题翻译：含有D-阿拉伯糖醇脱氢酶和NAD +的试剂盒用于测定D-阿拉伯糖醇
公开(公告)号：US5766874A
公开(公告)日：1998-06-16
申请号：US479069
申请日：1995-06-07
申请人： Charles Garrett Miyada , Arthur C. Switchenko , Melanie W. Quong , Man-Ying Laurie Wong
发明人： Charles Garrett Miyada , Arthur C. Switchenko , Melanie W. Quong , Man-Ying Laurie Wong
IPC分类号： C12N9/02 , C12N9/04 , C12N15/02 , C12P19/02 , C12P21/08 , C12Q1/04 , C12Q1/32 , C12R1/72 , C12R1/74 , C12R1/91 , G01N33/53 , G01N33/569 , G01N33/573 , G01N33/577 , C12N1/16
CPC分类号： C12N9/0006 , C12P19/02 , Y10S435/81 , Y10S435/921 , Y10S435/924
摘要： A D-arabinitol dehydrogenase enzyme is prepared that is capable of catalyzing the oxidation of D-arabinitol and substantially incapable of catalyzing the oxidation of D-mannitol and is substantially free of other enzymes capable of oxidizing D-mannitol. The enzyme is preferably obtained from Candida tropicalis ATCC 750 or Candida shehatae. The enzyme may also be obtained by recombinant DNA technology. Monoclonal antibodies are produced against the enzyme and can be used to identify the enzyme. The enzyme is used for detecting the presence of Candida in a host such as detecting Candida infection in a patient by determining the presence of D-arabinitol in a sample. The oxidation of D-arabinitol by nicotinamide adenine dinucleotide (NAD.sup.+) is catalyzed by the enzyme and the amount of NADH produced in a given time is determined. Also prepared is a composition containing the enzyme and NAD.sup.+ and a kit containing a packaged combination of the enzyme and NAD.sup.+.
摘要翻译：制备能够催化D-阿拉伯糖醇氧化并且基本上不能催化D-甘露糖醇的氧化并且基本上不含能够氧化D-甘露糖醇的其它酶的D-阿拉伯糖醇脱氢酶。该酶优选获自热带假丝酵母ATCC 750或念珠菌（念珠菌）。该酶也可以通过重组DNA技术获得。针对酶产生单克隆抗体，可用于鉴定酶。该酶用于通过测定样品中D-阿拉伯糖醇的存在来检测宿主中假丝酵母的存在，例如检测患者中的念珠菌感染。由烟酰胺腺嘌呤二核苷酸（NAD +）氧化D-阿拉伯糖醇由酶催化，并确定在给定时间内产生的NADH的量。还制备了含有酶和NAD +的组合物和含有酶和NAD +的包装组合的试剂盒。

4. 发明授权

US06426204B1 Method for detecting candida infection 失效
标题翻译：检测念珠菌感染的方法
公开(公告)号：US06426204B1
公开(公告)日：2002-07-30
申请号：US08476394
申请日：1995-06-07
申请人： Charles Garrett Miyada , Arthur C. Switchenko , Melanie W Quong , Man-Ying Laurie Wong
发明人： Charles Garrett Miyada , Arthur C. Switchenko , Melanie W Quong , Man-Ying Laurie Wong
IPC分类号： C12N904
CPC分类号： C12N9/0006 , C12P19/02 , Y10S435/81 , Y10S435/921 , Y10S435/924
摘要： A D-arabinitol dehydrogenase enzyme is disclosed. The enzyme is capable of catalyzing the oxidation of D-arabinitol and substantially incapable of catalyzing the oxidation of D-mannitol and is substantially free of other enzymes capable of oxidizing D-mannitol. Also disclosed are methods for determining D-arabinitol. In one embodiment the method comprises the steps of providing in combination (1) a medium suspected of containing D-arabinitol and (2) a D-arabinitol dehydrogenase enzyme and examining the medium for the product of the oxidation of the D-arabinitol. The enzyme utilized is capable of catalyzing the oxidation of D-arabinitol and substantially incapable of catalyzing the oxidation of D-mannitol. Kits for conducting the present method are also disclosed.
摘要翻译：公开了D-阿拉伯糖醇脱氢酶。该酶能够催化D-阿拉伯糖醇的氧化，并且基本上不能催化D-甘露糖醇的氧化，并且基本上不含能够氧化D-甘露糖醇的其它酶。还公开了测定D-阿拉伯糖醇的方法。在一个实施方案中，该方法包括以下步骤：组合（1）怀疑含有D-阿拉伯糖醇的培养基和（2）D-阿拉伯糖醇脱氢酶，并检查培养基中D-阿拉伯糖醇的氧化产物。所使用的酶能够催化D-阿拉伯糖醇的氧化，并且基本上不能催化D-甘露糖醇的氧化。还公开了用于进行本方法的试剂盒。

5. 发明授权

US6156501A Arrays of modified nucleic acid probes and methods of use 失效
标题翻译：修饰的核酸探针的阵列和使用方法
公开(公告)号：US6156501A
公开(公告)日：2000-12-05
申请号：US630427
申请日：1996-04-03
申请人： Glenn Hugh McGall , Charles Garrett Miyada , Maureen T. Cronin , Jennifer Dee Tan , Mark S. Chee
发明人： Glenn Hugh McGall , Charles Garrett Miyada , Maureen T. Cronin , Jennifer Dee Tan , Mark S. Chee
IPC分类号： C07H21/00 , C12Q1/68
CPC分类号： C07H21/00
摘要： Oligonucleotide analogue arrays attached to solid substrates and methods related to the use thereof are provided. The oligonucleotide analogues hybridize to nucleic acids with either higher or lower specificity than corresponding unmodified oligonucleotides. Target nucleic acids which comprise nucleotide analogues are bound to oligonucleotide and oligonucleotide analogue arrays.
摘要翻译：提供了连接到固体底物的寡核苷酸类似物阵列和与使用相关的方法。寡核苷酸类似物与具有比相应的未修饰寡核苷酸更高或更低特异性的核酸杂交。包含核苷酸类似物的靶核酸与寡核苷酸和寡核苷酸类似物阵列结合。

6. 发明授权

US6027880A Arrays of nucleic acid probes and methods of using the same for detecting cystic fibrosis 失效
标题翻译：核酸探针阵列及其用于检测囊性纤维化的方法
公开(公告)号：US6027880A
公开(公告)日：2000-02-22
申请号：US544381
申请日：1995-10-10
申请人： Maureen T. Cronin , Charles Garrett Miyada , Earl A. Hubbell , Mark Chee , Stephen P. A. Fodor , Xiaohua C. Huang , Robert J. Lipshutz , Peter E. Lobban , Macdonald S. Morris , Edward L. Sheldon
发明人： Maureen T. Cronin , Charles Garrett Miyada , Earl A. Hubbell , Mark Chee , Stephen P. A. Fodor , Xiaohua C. Huang , Robert J. Lipshutz , Peter E. Lobban , Macdonald S. Morris , Edward L. Sheldon
IPC分类号： B01J19/00 , C07B61/00 , C07H21/00 , C12Q1/68 , C40B40/06 , C40B60/14
CPC分类号： C07H21/00 , B01J19/0046 , B82Y30/00 , C12Q1/6827 , C12Q1/6837 , C12Q1/6869 , C12Q1/6874 , C12Q1/6883 , B01J2219/00432 , B01J2219/00529 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , C07B2200/11 , C12Q1/6886 , C12Q2600/106 , C12Q2600/156 , C40B40/06 , C40B60/14
摘要： The invention provides arrays of immobilized probes, and methods employing the arrays, for detecting mutations in the CFTR gene.
摘要翻译：本发明提供了固定化探针的阵列，以及采用阵列的方法，用于检测CFTR基因中的突变。

7. 发明授权

US07375198B2 Modified nucleic acid probes 失效
标题翻译：修饰的核酸探针
公开(公告)号：US07375198B2
公开(公告)日：2008-05-20
申请号：US10418414
申请日：2003-04-18
申请人： Glenn Hugh McGall , Charles Garrett Miyada , Maureen T. Cronin , Jennifer Dee Tan , Mark S. Chee
发明人： Glenn Hugh McGall , Charles Garrett Miyada , Maureen T. Cronin , Jennifer Dee Tan , Mark S. Chee
IPC分类号： C07H21/00 , C07H21/02 , C12Q1/68
CPC分类号： C40B80/00 , B01J19/0046 , B01J2219/00432 , B01J2219/00529 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C07B2200/11 , C07H21/00 , C12Q1/6832 , C40B40/06 , C40B60/14
摘要： Oligonucleotide analogue arrays attached to solid substrates and methods related to the use thereof are provided. The oligonucleotide analogues hybridize to nucleic acids with either higher or lower specificity than corresponding unmodified oligonucleotides. Target nucleic acids which comprise nucleotide analogues are bound to oligonucleotide and oligonucleotide analogue arrays.
摘要翻译：提供了连接到固体底物的寡核苷酸类似物阵列和与使用相关的方法。寡核苷酸类似物与具有比相应的未修饰寡核苷酸更高或更低特异性的核酸杂交。包含核苷酸类似物的靶核酸与寡核苷酸和寡核苷酸类似物阵列结合。

8. 发明授权

US6020461A Hexapeptides of Neisseria gonorrhoeae 失效
标题翻译：淋病奈瑟氏球菌的六肽
公开(公告)号：US6020461A
公开(公告)日：2000-02-01
申请号：US452915
申请日：1995-05-30
申请人： Charles Garrett Miyada , Teresa L. Born
发明人： Charles Garrett Miyada , Teresa L. Born
IPC分类号： C07K7/06 , C07K16/12 , C12N15/31 , C12Q1/68
CPC分类号： C12Q1/689 , Y10S435/822 , Y10S435/871
摘要： A nucleotide sequence characteristic of Neisseria gonorrhoeae is disclosed. The sequence can be the basis for hybridization type, nucleic acid-based, rapid, in vitro diagnostic assays. The unique nature of the sequence makes it possible to clearly discriminate N. gonorrhoeae from other Neisseria species thus eliminating or substantially reducing the number of false positive readings. A 350 base pair N. gonorrhoeae DNA restriction fragment was cloned after subtractive hybridization to Neisseria meningitidis DNA. In further cloning experiments the sequences adjacent to the original 350 base pair fragment were determined. A portion of this sequence was shown to detect 105 of 106 N. gonorrhoeae strains and no other Neisseria species. In addition to use as detection probes, all or portions of the nucleotide sequence can be used as a ligand for the sandwich capture of N. gonorrhoeae sequences and as primers for in vitro amplification of N. gonorrhoeae sequences. The polypeptides encoded by the presently disclosed sequence, including antibodies thereto, are also disclosed as are their uses.
摘要翻译：披露了淋病奈瑟氏球菌特征的核苷酸序列。该序列可以是杂交类型，基于核酸，快速，体外诊断测定的基础。序列的独特性质使得可以清楚地区分淋病奈瑟氏球菌与其他奈瑟氏菌属物种，从而消除或显着减少假阳性读数的数量。在与奈瑟氏球菌脑膜炎奈瑟菌DNA消减杂交后克隆了350碱基对淋病奈瑟氏球菌DNA限制性片段。在进一步克隆实验中，确定与原始350碱基对片段相邻的序列。该序列的一部分显示检测到106个淋病奈瑟氏球菌菌株中的105个，并且没有其他的奈瑟球菌属物种。除了用作检测探针之外，核苷酸序列的全部或部分可以用作奈氏淋病奈瑟氏球菌序列的夹心捕获的配体，并且用作用于体外扩增淋病奈瑟氏球菌序列的引物。本文公开的序列编码的多肽（包括其抗体）也被用于其用途。

9. 发明授权

US07115364B1 Arrays of nucleic acid probes on biological chips 失效
标题翻译：生物芯片上的核酸探针阵列
公开(公告)号：US07115364B1
公开(公告)日：2006-10-03
申请号：US08510521
申请日：1995-08-02
申请人： Mark Chee , Maureen T. Cronin , Stephen P. A. Fodor , Thomas R. Gingeras , Xiaohua C. Huang , Earl A. Hubbell , Robert J. Lipshutz , Peter E. Lobban , Charles Garrett Miyada , Macdonald S. Morris , Nila Shah , Edward L. Sheldon
发明人： Mark Chee , Maureen T. Cronin , Stephen P. A. Fodor , Thomas R. Gingeras , Xiaohua C. Huang , Earl A. Hubbell , Robert J. Lipshutz , Peter E. Lobban , Charles Garrett Miyada , Macdonald S. Morris , Nila Shah , Edward L. Sheldon
IPC分类号： C12Q1/68 , G01N33/50 , G01N33/53 , C07H21/04
CPC分类号： C12Q1/6869 , C12Q1/6886 , Y02A50/54
摘要： The invention provides chips of immobilized probes, and methods employing the chips, for comparing a reference polynucleotide sequence of known sequence with a target sequence showing substantial similarity with the reference sequence, but differing in the presence of e.g., mutations.
摘要翻译：本发明提供了固定化探针的芯片，以及使用该芯片的方法，用于将已知序列的参考多核苷酸序列与显示与参考序列基本相似的靶序列进行比较，但在例如突变的存在下不同。

10. 发明申请

US20120329677A9 ARRAYS OF NUCLEIC ACID PROBES FOR DETECTING CYSTIC FIBROSIS 审中-公开
标题翻译：用于检测CYSTIC FIBROSIS的核酸探针阵列
公开(公告)号：US20120329677A9
公开(公告)日：2012-12-27
申请号：US11506176
申请日：2006-08-18
申请人： Maureen T. Cronin , Charles Garrett Miyada , Earl A. Hubbell , Mark Chee , Stephen P.A. Fodor , Xiaohua C. Huang , Robert J. Lipshutz , Peter E. Lobban , MacDonald S. Morris , Edward L. Sheldon
发明人： Maureen T. Cronin , Charles Garrett Miyada , Earl A. Hubbell , Mark Chee , Stephen P.A. Fodor , Xiaohua C. Huang , Robert J. Lipshutz , Peter E. Lobban , MacDonald S. Morris , Edward L. Sheldon
IPC分类号： C40B40/06 , C40B50/14
CPC分类号： C12Q1/6874 , C12Q1/6886
摘要： The invention provides arrays of immobilized probes, and methods employing the arrays, for detecting mutations in the CFTR gene.
摘要翻译：本发明提供了固定化探针的阵列，以及采用阵列的方法，用于检测CFTR基因中的突变。

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