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1. 发明申请

US20080118972A1 METHOD AND APPARATUS FOR ISOLATING AND PURIFYING NUCLEIC ACID USING A SINGLE SURFACE 有权
标题翻译：使用单面表面分离和纯化核酸的方法和装置
公开(公告)号：US20080118972A1
公开(公告)日：2008-05-22
申请号：US11693259
申请日：2007-03-29
申请人： Chang eun YOO , Sung-young JEONG , Young-rok KIM
发明人： Chang eun YOO , Sung-young JEONG , Young-rok KIM
IPC分类号： C12N1/08 , C08F20/02 , C08F26/06 , C12M1/00
CPC分类号： B01J20/28009 , B01D15/1807 , B01J20/3204 , B01J20/321 , B01J20/3212 , B01J20/3219 , B01J20/327 , C08F20/02 , C08F26/06 , C12N1/066 , C12N13/00
摘要： Provided is a method of isolating nucleic acid from cells using a single surface, wherein a compound represented by Formula 1 is bound to the surface. Also provided are an apparatus for isolation of nucleic acids, and a bead for isolating nucleic acids.
摘要翻译：提供了使用单个表面从细胞中分离核酸的方法，其中由式1表示的化合物结合到表面。还提供了用于分离核酸的装置和用于分离核酸的珠粒。

2. 发明申请

US20100160585A1 METHOD AND APPARATUS FOR ISOLATING AND PURIFYING NUCLEIC ACID USING A SINGLE SURFACE 有权
标题翻译：使用单面表面分离和纯化核酸的方法和装置
公开(公告)号：US20100160585A1
公开(公告)日：2010-06-24
申请号：US12716695
申请日：2010-03-03
申请人： Chang eun YOO , Sung-young JEONG , Young-rok KIM
发明人： Chang eun YOO , Sung-young JEONG , Young-rok KIM
IPC分类号： C08F226/06
CPC分类号： B01J20/28009 , B01D15/1807 , B01J20/3204 , B01J20/321 , B01J20/3212 , B01J20/3219 , B01J20/327 , C08F20/02 , C08F26/06 , C12N1/066 , C12N13/00
摘要： Provided is a method of isolating nucleic acid from cells using a single surface, wherein a compound represented by Formula 1 is bound to the surface. Also provided are an apparatus for isolation of nucleic acids, and a bead for isolating nucleic acids.
摘要翻译：提供了使用单个表面从细胞中分离核酸的方法，其中由式1表示的化合物结合到表面。还提供了用于分离核酸的装置和用于分离核酸的珠粒。

3. 发明申请

US20090005265A1 METHOD OF ISOLATING NUCLEIC ACID USING MATERIAL POSITIVELY CHARGED AT FIRST PH AND CONTAINING AMINO GROUP AND CARBOXYL GROUP 有权
标题翻译：使用在第一个PH和含有氨基和羧基的正电荷充填材料分离核酸的方法
公开(公告)号：US20090005265A1
公开(公告)日：2009-01-01
申请号：US12141894
申请日：2008-06-18
申请人： Kyu-youn HWANG , Joon-ho KIM , Chang-eun YOO , Hun-joo LEE , Hee-kyun LIM , Sung-young JEONG , Jeo-young SHIM
发明人： Kyu-youn HWANG , Joon-ho KIM , Chang-eun YOO , Hun-joo LEE , Hee-kyun LIM , Sung-young JEONG , Jeo-young SHIM
IPC分类号： C40B40/04 , C07C63/26
CPC分类号： C12N15/1006 , B01D15/361 , B01D15/364 , B01J20/3285 , B01J43/00 , C12Q1/6806 , C12Q2527/119 , C12Q2523/308
摘要： A method of isolating nucleic acid from a sample containing nucleic acid is provided. The method includes contacting the sample with a bifunctional material that contains an amino group and a carboxyl group and is positively charged at a first pH to allow binding of the nucleic acid to the bifunctional material; and extracting the nucleic acid at a second pH higher than the first pH from the complex.
摘要翻译：提供了从含有核酸的样品中分离核酸的方法。该方法包括使样品与含有氨基和羧基的双官能材料接触并在第一pH下带正电荷以允许核酸与双功能材料的结合; 以及从所述络合物中提取比所述第一pH高的第二pH的所述核酸。

4. 发明申请

US20080009043A1 MICROFLUIDIC DEVICE INCLUDING A MICROCHANNEL WITH A HYDROPHOBIC POROUS POLYMER BONDED TO WALLS THEREOF AND TO A MAGNETIC BEAD, AND METHODS OF MAKING AND USING THE DEVICE 有权
标题翻译：微流控器件，其中包含与疏水性多孔聚合物粘合的微通道和磁性珠，以及制造和使用器件的方法
公开(公告)号：US20080009043A1
公开(公告)日：2008-01-10
申请号：US11675840
申请日：2007-02-16
申请人： Chang-eun YOO , Jong-myeon PARK , Sung-young JEONG
发明人： Chang-eun YOO , Jong-myeon PARK , Sung-young JEONG
IPC分类号： C12P19/34 , C12M1/34 , C12Q1/68
CPC分类号： C12M47/06 , B01L3/502761 , B01L7/52 , B01L2200/0631 , B01L2200/0668 , B01L2200/10 , B01L2300/069 , B01L2300/087 , B01L2300/165 , B01L2300/18 , B01L2400/043 , C12M23/16
摘要： This invention provides a microfluidic device comprising an inlet and an outlet which are connected with each other through a microchannel, wherein a polymerized hydrophobic porous polymer is bonded to magnetic beads and to the walls of the microchannel. The invention is further directed to methods of making and using the microfluidic device.
摘要翻译：本发明提供了一种微流体装置，其包括通过微通道彼此连接的入口和出口，其中聚合的疏水性多孔聚合物结合到磁珠和微通道的壁上。本发明进一步涉及制造和使用微流体装置的方法。

5. 发明申请

US20080070282A1 METHOD AND DEVICE FOR OBTAINING OR AMPLIFYING NUCLEIC ACID FROM A CELL USING A NONPLANAR SOLID SUBSTRATE 审中-公开
标题翻译：使用非固体基质从细胞中获得或放大核酸的方法和装置
公开(公告)号：US20080070282A1
公开(公告)日：2008-03-20
申请号：US11841069
申请日：2007-08-20
申请人： Kyu-youn HWANG , Sung-young JEONG , Joon-ho KIM , Jung-im HAN
发明人： Kyu-youn HWANG , Sung-young JEONG , Joon-ho KIM , Jung-im HAN
IPC分类号： C12P19/34 , C12M1/00
CPC分类号： C12N15/1006 , B01L7/52 , B01L2300/0636 , C12N2533/30 , C12Q1/6806
摘要： The present invention relates to a method of isolating a nucleic acid from a microorganism cell. The method comprises contacting a nonplanar solid substrate with a cell-containing sample in a liquid medium having a pH of 3.0 to 6.0 so that the microorganism cell is bound to the nonplanar solid substrate, and lysing the microorganism cell bound to the nonplanar solid substrate. The invention also relates a device including the nonplanar solid substrate for isolating and amplifying a nucleic acid.
摘要翻译：本发明涉及从微生物细胞分离核酸的方法。该方法包括使非平面固体基质与含有细胞的样品在pH为3.0至6.0的液体培养基中接触，使得微生物细胞与非平面固体底物结合，并溶解与非平面固体底物结合的微生物细胞。本发明还涉及一种包括用于分离和扩增核酸的非平面固体底物的装置。

6. 发明申请

US20120255871A1 APPARATUS AND METHOD FOR ADJUSTING PH OF SOLUTION 有权
标题翻译：用于调节溶液PH的装置和方法
公开(公告)号：US20120255871A1
公开(公告)日：2012-10-11
申请号：US13495342
申请日：2012-06-13
申请人： Hun-joo LEE , Jin-tae KIM , Sung-young JEONG , Joon-ho KIM , Hee-kyun LIM
发明人： Hun-joo LEE , Jin-tae KIM , Sung-young JEONG , Joon-ho KIM , Hee-kyun LIM
IPC分类号： C25B15/00
CPC分类号： G05D21/02
摘要： A pH adjusting apparatus includes an electrolytic chip receiving a solution, an electrolytic chip loading station receiving the electrolytic chip, an input unit inputting electrolysis conditions, a control unit receiving the electrolysis conditions and controlling electrolysis performed in the electrolytic chip, and a display unit displaying the electrolysis conditions and a progress of the electrolysis. Thus, the pH of a solution can be adjusted easily and accurately, by precisely controlling a constant current, a constant voltage, and current and voltage application times, thereby enabling useful application in various biological assays such as cell lysis. Furthermore, the pH adjusting apparatus has small size and weight and can be operated for a long time after charging once due to low power consumption.
摘要翻译： pH调节装置包括：接收溶液的电解芯片，接收电解芯片的电解芯片装载站，输入电解条件的输入单元，接收电解条件的控制单元以及控制电解芯片中进行的电解;以及显示单元，电解条件和电解进度。因此，通过精确地控制恒定电流，恒定电压，电流和电压施加时间，可以容易且精确地调节溶液的pH，从而可用于各种生物测定如细胞裂解中的有用应用。此外，pH调节装置的体积小，重量轻，能够在低功耗的情况下一次充电后长时间运转。

7. 发明申请

US20120088286A1 METHOD OF SEPARATING MICROORGANISM USING NONPLANAR SOLID SUBSTRATE AND DEVICE FOR SEPARATING MICROORGANISM USING THE SAME 有权
标题翻译：使用非固体基质分离微生物的方法和使用其分离微生物的装置
公开(公告)号：US20120088286A1
公开(公告)日：2012-04-12
申请号：US13325980
申请日：2011-12-14
申请人： Kyu-youn HWANG , Sung-young JEONG , Joon-ho KIM
发明人： Kyu-youn HWANG , Sung-young JEONG , Joon-ho KIM
IPC分类号： C12N11/08 , C12N11/02 , C12N11/00
CPC分类号： C12N1/02 , B01J39/02 , B01J39/14
摘要： Provided is a method of separating microorganisms from a sample including contacting the sample containing microorganisms with an inorganic on exchange material such that the sample reacts with the inorganic on exchange material, and contacting the reacted sample with a means for capturing microorganisms.
摘要翻译：提供了一种从样品中分离微生物的方法，包括使含有微生物的样品与交换材料上的无机物接触，使得样品与无机的交换材料反应，并使反应的样品与捕获微生物的装置接触。

8. 发明申请

US20070151864A1 APPARATUS AND METHOD FOR ADJUSTING PH OF SOLUTION 有权
标题翻译：用于调节溶液PH的装置和方法
公开(公告)号：US20070151864A1
公开(公告)日：2007-07-05
申请号：US11559924
申请日：2006-11-15
申请人： Hun-joo LEE , Jin-tae KIM , Sung-young JEONG , Joon-ho KIM , Hee-kyun LIM
发明人： Hun-joo LEE , Jin-tae KIM , Sung-young JEONG , Joon-ho KIM , Hee-kyun LIM
IPC分类号： C25B1/00
CPC分类号： G05D21/02
摘要： A pH adjusting apparatus includes an electrolytic chip receiving a solution, an electrolytic chip loading station receiving the electrolytic chip, an input unit inputting electrolysis conditions, a control unit receiving the electrolysis conditions and controlling electrolysis performed in the electrolytic chip, and a display unit displaying the electrolysis conditions and a progress of the electrolysis. Thus, the pH of a solution can be adjusted easily and accurately, by precisely controlling a constant current, a constant voltage, and current and voltage application times, thereby enabling useful application in various biological assays such as cell lysis. Furthermore, the pH adjusting apparatus has small size and weight and can be operated for a long time after charging once due to low power consumption.
摘要翻译： pH调节装置包括：接收溶液的电解芯片，接收电解芯片的电解芯片装载站，输入电解条件的输入单元，接收电解条件的控制单元以及控制电解芯片中进行的电解;以及显示单元，电解条件和电解进度。因此，通过精确地控制恒定电流，恒定电压，电流和电压施加时间，可以容易且精确地调节溶液的pH，从而可用于各种生物测定如细胞裂解中的有用应用。此外，pH调节装置的体积小，重量轻，能够在低功耗的情况下一次充电后长时间运转。

9. 发明申请

US20110081280A1 MICROFLUIDIC CHIP AND METHOD OF FABRICATING THE SAME 有权
标题翻译：微流控芯片及其制造方法
公开(公告)号：US20110081280A1
公开(公告)日：2011-04-07
申请号：US12949981
申请日：2010-11-19
申请人： Kyu-youn HWANG , Joon-ho KIM , Chin-sung PARK , Sung-young JEONG
发明人： Kyu-youn HWANG , Joon-ho KIM , Chin-sung PARK , Sung-young JEONG
IPC分类号： B01L3/00
CPC分类号： B01L3/502707 , B01L3/502753 , B01L2200/12 , B01L2300/16 , Y10T428/14
摘要： Provided are a microfluidic chip and a method of fabricating the same. The microfluidic chip includes: a lower substrate; an upper substrate formed of a silicone resin, wherein the lower substrate and the upper substrate, bonded together, provide a channel through which a fluid can flow and a chamber to receive the fluid; and an organic thin film formed on the upper surface of the lower substrate except for portions on which the lower substrate and the upper substrate are attached to each other.
摘要翻译：提供了一种微流控芯片及其制造方法。微流体芯片包括：下基板; 由硅树脂形成的上基板，其中所述下基板和所述上基板接合在一起，提供流体可以流过的通道和容纳所述流体的室; 以及形成在下基板的上表面上的有机薄膜，除了下基板和上基板彼此附接的部分之外。

10. 发明申请

US20070152206A1 DEVICE FOR MANIPULATING PARTICLES USING DIELECTROPHORESIS EMPLOYING METAL-POST ELECTRODE STRUCTURE AND METHOD OF MANIPULATING PARTICLES USING THE DEVICE AT HIGH FLOW RATE 审中-公开
标题翻译：使用采用金属电极后电极结构的电介质处理颗粒的装置以及使用高流速下的装置处理颗粒的方法
公开(公告)号：US20070152206A1
公开(公告)日：2007-07-05
申请号：US11617978
申请日：2006-12-29
申请人： Yoon-kyoung CHO , Sung-young JEONG , Kak NAMKOONG , Chin-sung PARK
发明人： Yoon-kyoung CHO , Sung-young JEONG , Kak NAMKOONG , Chin-sung PARK
IPC分类号： H01L29/06
CPC分类号： B03C5/005 , B03C5/026
摘要： A device and method for manipulating particles using dielectrophoresis are disclosed. The device comprises a chamber comprising an inlet port, an outlet port, and metal post electrodes, and a power supply, wherein the metal post electrodes are arranged in at least two rows in a vertical position with respect to the flow of fluids, each row comprises at least two metal post electrodes, each odd row is wired to a metal pad through a metal line, and each even row is wired to another metal pad through a metal line, and the power supply is connected to the metal pads.
摘要翻译：公开了一种使用介电电泳操作颗粒的装置和方法。该装置包括一个包括入口端口，出口端口和金属柱状电极以及电源的腔室，其中金属柱状电极相对于流体流沿垂直位置布置成至少两排，每排包括至少两个金属柱电极，每个奇数行通过金属线连接到金属焊盘，并且每个偶数行通过金属线连接到另一个金属焊盘，并且电源连接到金属焊盘。

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