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1. 发明申请

WO2004053137A3 METHOD FOR PROTEIN PRODUCTION 审中-公开
标题翻译：蛋白质生产方法
公开(公告)号：WO2004053137A3
公开(公告)日：2004-08-12
申请号：PCT/CH0300810
申请日：2003-12-10
申请人： CYTOS BIOTECHNOLOGY AG , SAUDAN PHILIPPE , HENNECKE FRANK , BACHMANN MARTIN F , STERN DOROTHEE M
发明人： SAUDAN PHILIPPE , HENNECKE FRANK , BACHMANN MARTIN F , STERN DOROTHEE M
IPC分类号： C07K14/505 , C12N15/869 , C12N5/10
CPC分类号： C12N15/86 , A01K2267/01 , C07K14/505 , C12N15/85 , C12N15/8509 , C12N2710/16643 , C12N2800/108 , C12N2840/20
摘要： The present invention relates to the field of gene expression technology and the production of recombinant polypeptides and/or untranslated RNA molecules in host cells. In particular, the invention provides a method for the production of recombinant polypeptides and/or untranslated RNA molecules in host cells allowing the production of high amounts of those molecules in a very short time and with a high degree of reproducibility, in particular with respect to a, preferably, pre-defined standard and, thus, making the present invention particularly attractive for the production of proteins for clinical use and the production under Good Manufacturing Practice (GMP).
摘要翻译：本发明涉及基因表达技术领域和在宿主细胞中生产重组多肽和/或非翻译RNA分子。特别地，本发明提供了在宿主细胞中生产重组多肽和/或非翻译RNA分子的方法，允许在非常短的时间内以高度的重现性产生大量的这些分子，特别是关于优选预定义的标准，并且因此使本发明对于生产用于临床应用的蛋白质和良好生产规范（GMP）下的生产特别具有吸引力。

2. 发明申请

WO0142442A3 ACTIVATION OF ENDOGENOUS GENES BY GENOMIC INTRODUCTION OF A REPLICON 审中-公开
标题翻译：通过基因引物回报激活内源基因
公开(公告)号：WO0142442A3
公开(公告)日：2001-11-22
申请号：PCT/IB0001841
申请日：2000-12-08
申请人： CYTOS BIOTECHNOLOGY AG , HENNECKE FRANK , RENNER WOLFGANG A
发明人： HENNECKE FRANK , RENNER WOLFGANG A
IPC分类号： A61K48/00 , C07K14/505 , C12N15/85 , C12N15/86 , C12N15/90 , C07K14/18 , C12N5/10 , C12N7/04 , C12N9/12
CPC分类号： C12N15/85 , A01K2217/05 , A61K48/00 , C07K14/505 , C12N15/86 , C12N15/907 , C12N2770/36143 , C12N2800/30 , C12N2830/008 , C12N2830/42 , C12N2830/85 , C12N2840/20 , C12N2840/203
摘要： The present invention relates to methods for the modification of genomes of eukaryotic cells to alter the expression of endogenous genes. The invention also relates to recombinant eukaryotic host cells and polypeptides produced by the practice of the disclosed methods. The invention further relates to vector systems useful for modifying the genomes of eukaryotic cells to alter the expression of endogenous genes.
摘要翻译：本发明涉及用于修饰真核细胞基因组以改变内源基因表达的方法。本发明还涉及通过实践所公开的方法产生的重组真核宿主细胞和多肽。本发明还涉及可用于修饰真核细胞基因组以改变内源基因表达的载体系统。

3. 发明申请

WO2006125821A2 SCALABLE FERMENTATION PROCESS 审中-公开
标题翻译：可扩展的发酵过程
公开(公告)号：WO2006125821A2
公开(公告)日：2006-11-30
申请号：PCT/EP2006062628
申请日：2006-05-24
申请人： CYTOS BIOTECHNOLOGY AG , EMMERLING MARCEL , HENNECKE FRANK , PFRUENDER HOLGER , RHIEL MARTIN , STEINER PHILIPP
发明人： EMMERLING MARCEL , HENNECKE FRANK , PFRUENDER HOLGER , RHIEL MARTIN , STEINER PHILIPP
CPC分类号： C07K14/005 , A61K2039/5256 , A61K2039/5258 , C12N7/00 , C12N2795/00023 , C12N2795/00051 , C12N2795/10051 , C12N2795/10061 , C12N2795/18022 , C12N2795/18052
摘要： This invention provides a robust fermentation process for the expression of a capsid protein of a bacteriophage which is forming a VLP by self-assembly, wherein the process is scalable to a commercial production scale and wherein the expression rate of the capsid protein is controlled to obtain improved yield of soluble capsid protein. This is achieved by combining the advantages of fed-batch culture and of lactose induced expression systems with specific process parameters providing improved repression of the promoter during the growth phase and high plasmid retention throughout the process.
摘要翻译：本发明提供了用于通过自组装形成VLP的噬菌体的衣壳蛋白的表达的鲁棒发酵方法，其中该方法可扩展到商业生产规模，并且其中控制衣壳蛋白的表达速率以获得提高可溶性衣壳蛋白的产量。这是通过将补料分批培养和乳糖诱导表达系统的优点与具体工艺参数相结合来实现的，其提供了在生长阶段改进的启动子抑制以及整个过程中的高质粒保留。

4. 发明申请

WO0032227A3 ORDERED MOLECULAR PRESENTATION OF ANTIGENS, METHOD OF PREPARATION AND USE 审中-公开
标题翻译：有序分子抗原的呈递，制备和使用方法
公开(公告)号：WO0032227A3
公开(公告)日：2000-10-12
申请号：PCT/IB9901925
申请日：1999-11-30
申请人： CYTOS BIOTECHNOLOGY AG
发明人： RENNER WOLFGANG A , HENNECKE FRANK , NIEBA LARS , BACHMANN MARTIN
IPC分类号： A61K47/00 , A61K39/00 , A61P31/00 , A61P35/00 , A61P37/02 , C12N7/01 , C12N5/12
CPC分类号： A61K39/00 , A61K2039/5256 , A61K2039/5258
摘要： The invention provides compositions and processes for the production of ordered and repetitive antigen or antigenic determinant arrays. The compositions of the invention are useful for the production of vaccines for the prevention of infectious diseases, the treatment of allergies and the treatment of cancers. Various embodiments of the invention provide for a virus, virus-like particle, viral capsid particle, phage or recombinant form thereof coated with any desired antigen in a highly ordered and repetitive fashion as the result of specific interactions. In one specific embodiment, a versatile new technology based on a cassette-type system (Alpha Vaccine Technology) allows production of antigen coated viral particles. Other specific embodiments allow the production of antigen coated hepatitis B virus-like particles or antigen coated Measles virus-like particles.
摘要翻译：本发明提供了用于生产有序和重复的抗原或抗原决定簇阵列的组合物和方法。本发明的组合物可用于生产用于预防感染性疾病，治疗变态反应和治疗癌症的疫苗。作为特定相互作用的结果，本发明的各种实施方案提供了以高度有序且重复的方式涂覆有任何期望的抗原的病毒，病毒样颗粒，病毒衣壳颗粒，噬菌体或其重组形式。在一个具体实施方式中，基于盒式系统（Alpha疫苗技术）的多功能新技术允许生产抗原包被的病毒颗粒。其他具体实施方案允许产生抗原包被的乙型肝炎病毒样颗粒或抗原包被的麻疹病毒样颗粒。

5. 发明专利

AT412434T 未知
公开(公告)号：AT412434T
公开(公告)日：2008-11-15
申请号：AT99972928
申请日：1999-11-30
申请人： CYTOS BIOTECHNOLOGY AG
发明人： RENNER WOLFGANG , HENNECKE FRANK , NIEBA LARS , BACHMANN MARTIN
IPC分类号： A61K47/00 , A61K39/00 , A61P31/00 , A61P35/00 , A61P37/02 , C12N5/12 , C12N7/01
摘要： The invention provides compositions and processes for the production of ordered and repetitive antigen or antigenic determinant arrays. The compositions of the invention are useful for the production of vaccines for the prevention of infectious diseases, the treatment of allergies and the treatment of cancers. Various embodiments of the invention provide for a virus, virus-like particle, viral capsid particle, phage or recombinant form thereof coated with any desired antigen in a highly ordered and repetitive fashion as the result of specific interactions. In one specific embodiment, a versatile new technology based on a cassette-type system (AlphaVaccine Technology) allows production of antigen coated viral particles. Other specific embodiments allow the production of antigen coated hepatitis B virus-like particles or antigen coated Measles virus-like particles.

6. 发明专利

ES2369405T3 PROCESO DE FERMANTACION ESCALABLE. 未知
公开(公告)号：ES2369405T3
公开(公告)日：2011-11-30
申请号：ES06777243
申请日：2006-05-24
申请人： CYTOS BIOTECHNOLOGY AG
发明人： EMMERLING MARCEL , HENNECKE FRANK , PFRUENDER HOLGER , RHIEL MARTIN , STEINER PHILIPP
IPC分类号： C12N7/02 , C12P21/02
摘要： Proceso para la expresión de una proteína de la cápside recombinante de un bacteriófago o un mutante o fragmento de la misma capaz de formar una VLP mediante auto-ensamblaje, en donde dicho bacteriófago es un bacteriófago de ARN, y en donde dicho proceso comprende los pasos de: a.) introducir un plásmido de expresión en un huésped bacteriano, en donde dicho plásmido de expresión comprende un constructo de expresión, en donde dicho constructo de expresión comprende (i) una primera secuencia de nucleótido que codifica dicha proteína de la cápside recombinante, o mutante o fragmento de la misma, y (ii) un promotor que es inducible por lactosa; b) cultivar dicho huésped bacteriano en un medio que comprende una fuente de carbono principal; en donde dicha fuente de carbono principal es glucosa o glicerol; y en donde dicho cultivo se realiza en cultivo discontinuo y en condiciones bajo las cuales dicho promotor se reprime por lacl, en donde dicho lacl es sobreexpresado por dicho huésped bacteriano; c) alimentar dicho cultivo discontinuo con dicha fuente de carbono principal; y d) inducir dicho promotor con un inductor, en donde se continúa dicha alimentación de dicho cultivo discontinuo con dicha fuente de carbono principal.

7. 发明专利

DK1135162T3 未知
公开(公告)号：DK1135162T3
公开(公告)日：2009-01-19
申请号：DK99972928
申请日：1999-11-30
申请人： CYTOS BIOTECHNOLOGY AG
发明人： RENNER WOLFGANG A , NIEBA LARS , HENNECKE FRANK , BACHMANN MARTIN
IPC分类号： A61K47/00 , A61K39/00 , A61P31/00 , A61P35/00 , A61P37/02 , C12N5/12 , C12N7/01
摘要： The invention provides compositions and processes for the production of ordered and repetitive antigen or antigenic determinant arrays. The compositions of the invention are useful for the production of vaccines for the prevention of infectious diseases, the treatment of allergies and the treatment of cancers. Various embodiments of the invention provide for a virus, virus-like particle, viral capsid particle, phage or recombinant form thereof coated with any desired antigen in a highly ordered and repetitive fashion as the result of specific interactions. In one specific embodiment, a versatile new technology based on a cassette-type system (AlphaVaccine Technology) allows production of antigen coated viral particles. Other specific embodiments allow the production of antigen coated hepatitis B virus-like particles or antigen coated Measles virus-like particles.

8. 发明专利

CA2608579A1 SCALABLE FERMENTATION PROCESS 未知
公开(公告)号：CA2608579A1
公开(公告)日：2006-11-30
申请号：CA2608579
申请日：2006-05-24
申请人： CYTOS BIOTECHNOLOGY AG
发明人： EMMERLING MARCEL , STEINER PHILIPP , RHIEL MARTIN , HENNECKE FRANK , PFRUENDER HOLGER
IPC分类号： C12N7/02 , C12P21/02
摘要： This invention provides a robust fermentation process for the expression of a capsid protein of a bacteriophage which is forming a VLP by self-assembly, wherein the process is scalable to a commercial production scale and wherein the expression rate of the capsid protein is controlled to obtain improved yield of soluble capsid protein. This is achieved by combining the advantage s of fed-batch culture and of lactose induced expression systems with specific process parameters providing improved repression of the promoter during the growth phase and high plasmid retention throughout the process.

9. 发明专利

ZA200105050B Ordered molecular presentation of antigens, method of preparation and use. 未知
公开(公告)号：ZA200105050B
公开(公告)日：2002-06-20
申请号：ZA200105050
申请日：2001-06-20
申请人： CYTOS BIOTECHNOLOGY AG
发明人： RENNER WOLFGANG A , HENNECKE FRANK , NIEBA LARS , BACHMANN MARTIN
IPC分类号： A61K20060101 , A61K

10. 发明专利

CA2608579C SCALABLE FERMENTATION PROCESS 未知
公开(公告)号：CA2608579C
公开(公告)日：2019-09-10
申请号：CA2608579
申请日：2006-05-24
申请人： CYTOS BIOTECHNOLOGY AG
发明人： EMMERLING MARCEL , HENNECKE FRANK , PFRUENDER HOLGER , RHIEL MARTIN , STEINER PHILIPP
IPC分类号： C12N7/02 , C12P21/02
摘要： This invention provides a robust fermentation process for the expression of a capsid protein of a bacteriophage which is forming a VLP by self-assembly, wherein the process is scalable to a commercial production scale and wherein the expression rate of the capsid protein is controlled to obtain improved yield of soluble capsid protein. This is achieved by combining the advantages of fed-batch culture and of lactose induced expression systems with specific process parameters providing improved repression of the promoter during the growth phase and high plasmid retention throughout the process.

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