专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2006125821A2 SCALABLE FERMENTATION PROCESS 审中-公开
标题翻译：可扩展的发酵过程
公开(公告)号：WO2006125821A2
公开(公告)日：2006-11-30
申请号：PCT/EP2006062628
申请日：2006-05-24
申请人： CYTOS BIOTECHNOLOGY AG , EMMERLING MARCEL , HENNECKE FRANK , PFRUENDER HOLGER , RHIEL MARTIN , STEINER PHILIPP
发明人： EMMERLING MARCEL , HENNECKE FRANK , PFRUENDER HOLGER , RHIEL MARTIN , STEINER PHILIPP
CPC分类号： C07K14/005 , A61K2039/5256 , A61K2039/5258 , C12N7/00 , C12N2795/00023 , C12N2795/00051 , C12N2795/10051 , C12N2795/10061 , C12N2795/18022 , C12N2795/18052
摘要： This invention provides a robust fermentation process for the expression of a capsid protein of a bacteriophage which is forming a VLP by self-assembly, wherein the process is scalable to a commercial production scale and wherein the expression rate of the capsid protein is controlled to obtain improved yield of soluble capsid protein. This is achieved by combining the advantages of fed-batch culture and of lactose induced expression systems with specific process parameters providing improved repression of the promoter during the growth phase and high plasmid retention throughout the process.
摘要翻译：本发明提供了用于通过自组装形成VLP的噬菌体的衣壳蛋白的表达的鲁棒发酵方法，其中该方法可扩展到商业生产规模，并且其中控制衣壳蛋白的表达速率以获得提高可溶性衣壳蛋白的产量。这是通过将补料分批培养和乳糖诱导表达系统的优点与具体工艺参数相结合来实现的，其提供了在生长阶段改进的启动子抑制以及整个过程中的高质粒保留。

2. 发明专利

CA2608579C SCALABLE FERMENTATION PROCESS 未知
公开(公告)号：CA2608579C
公开(公告)日：2019-09-10
申请号：CA2608579
申请日：2006-05-24
申请人： CYTOS BIOTECHNOLOGY AG
发明人： EMMERLING MARCEL , HENNECKE FRANK , PFRUENDER HOLGER , RHIEL MARTIN , STEINER PHILIPP
IPC分类号： C12N7/02 , C12P21/02
摘要： This invention provides a robust fermentation process for the expression of a capsid protein of a bacteriophage which is forming a VLP by self-assembly, wherein the process is scalable to a commercial production scale and wherein the expression rate of the capsid protein is controlled to obtain improved yield of soluble capsid protein. This is achieved by combining the advantages of fed-batch culture and of lactose induced expression systems with specific process parameters providing improved repression of the promoter during the growth phase and high plasmid retention throughout the process.

3. 发明专利

NZ563708A Scalable fermentation process 未知
公开(公告)号：NZ563708A
公开(公告)日：2010-08-27
申请号：NZ56370806
申请日：2006-05-24
申请人： CYTOS BIOTECHNOLOGY AG
发明人： EMMERLING MARCEL , HENNECKE FRANK , RHIEL MARTIN , STEINER PHILIPP , PFRUNDER HOLGER
IPC分类号： C12N7/02 , C12P21/02
摘要： A process for expression of a recombinant capsid protein of a bacteriophage or a mutant or fragment thereof being capable of forming a VLP by self-assembly, wherein said bacteriophage is a RNA bacteriophage, and wherein said process comprising the steps of: a.) introducing an expression plasmid into a bacterial host, wherein said expression plasmid comprises an expression construct, wherein said expression construct comprises (i) a first nucleotide sequence encoding said recombinant capsid protein, or mutant or fragment thereof, and (ii) a promoter being inducible by lactose; b.) cultivating said bacterial host in a medium comprising a major carbon source; wherein said major carbon source is glucose or glycerol; and wherein said cultivating is performed in batch culture and under conditions under which said promoter is repressed by lac!, wherein said lac! is overexpressed by said bacterial host; c.) feeding said batch culture with said major carbon source; and d.) inducing said promoter with an inducer, wherein said feeding of said batch culture with said major carbon source is continued.

4. 发明专利

AT515566T 未知
公开(公告)号：AT515566T
公开(公告)日：2011-07-15
申请号：AT06777243
申请日：2006-05-24
申请人： CYTOS BIOTECHNOLOGY AG
发明人： EMMERLING MARCEL , HENNECKE FRANK , PFRUENDER HOLGER , RHIEL MARTIN , STEINER PHILIPP
IPC分类号： C12N7/02 , C12P21/02
摘要： This invention provides a robust fermentation process for the expression of a capsid protein of a bacteriophage which is forming a VLP by self-assembly, wherein the process is scalable to a commercial production scale and wherein the expression rate of the capsid protein is controlled to obtain improved yield of soluble capsid protein. This is achieved by combining the advantages of fed-batch culture and of lactose induced expression systems with specific process parameters providing improved repression of the promoter during the growth phase and high plasmid retention throughout the process.

5. 发明专利

AU2006251098A1 Scalable fermentation process 未知
公开(公告)号：AU2006251098A1
公开(公告)日：2006-11-30
申请号：AU2006251098
申请日：2006-05-24
申请人： CYTOS BIOTECHNOLOGY AG
发明人： RHIEL MARTIN , EMMERLING MARCEL , STEINER PHILIPP , PFRUNDER HOLGER , HENNECKE FRANK
IPC分类号： C12N7/02 , C12P21/02
摘要： This invention provides a robust fermentation process for the expression of a capsid protein of a bacteriophage which is forming a VLP by self-assembly, wherein the process is scalable to a commercial production scale and wherein the expression rate of the capsid protein is controlled to obtain improved yield of soluble capsid protein. This is achieved by combining the advantages of fed-batch culture and of lactose induced expression systems with specific process parameters providing improved repression of the promoter during the growth phase and high plasmid retention throughout the process.

6. 发明专利

ES2369405T3 PROCESO DE FERMANTACION ESCALABLE. 未知
公开(公告)号：ES2369405T3
公开(公告)日：2011-11-30
申请号：ES06777243
申请日：2006-05-24
申请人： CYTOS BIOTECHNOLOGY AG
发明人： EMMERLING MARCEL , HENNECKE FRANK , PFRUENDER HOLGER , RHIEL MARTIN , STEINER PHILIPP
IPC分类号： C12N7/02 , C12P21/02
摘要： Proceso para la expresión de una proteína de la cápside recombinante de un bacteriófago o un mutante o fragmento de la misma capaz de formar una VLP mediante auto-ensamblaje, en donde dicho bacteriófago es un bacteriófago de ARN, y en donde dicho proceso comprende los pasos de: a.) introducir un plásmido de expresión en un huésped bacteriano, en donde dicho plásmido de expresión comprende un constructo de expresión, en donde dicho constructo de expresión comprende (i) una primera secuencia de nucleótido que codifica dicha proteína de la cápside recombinante, o mutante o fragmento de la misma, y (ii) un promotor que es inducible por lactosa; b) cultivar dicho huésped bacteriano en un medio que comprende una fuente de carbono principal; en donde dicha fuente de carbono principal es glucosa o glicerol; y en donde dicho cultivo se realiza en cultivo discontinuo y en condiciones bajo las cuales dicho promotor se reprime por lacl, en donde dicho lacl es sobreexpresado por dicho huésped bacteriano; c) alimentar dicho cultivo discontinuo con dicha fuente de carbono principal; y d) inducir dicho promotor con un inductor, en donde se continúa dicha alimentación de dicho cultivo discontinuo con dicha fuente de carbono principal.

7. 发明专利

CA2608579A1 SCALABLE FERMENTATION PROCESS 未知
公开(公告)号：CA2608579A1
公开(公告)日：2006-11-30
申请号：CA2608579
申请日：2006-05-24
申请人： CYTOS BIOTECHNOLOGY AG
发明人： EMMERLING MARCEL , STEINER PHILIPP , RHIEL MARTIN , HENNECKE FRANK , PFRUENDER HOLGER
IPC分类号： C12N7/02 , C12P21/02
摘要： This invention provides a robust fermentation process for the expression of a capsid protein of a bacteriophage which is forming a VLP by self-assembly, wherein the process is scalable to a commercial production scale and wherein the expression rate of the capsid protein is controlled to obtain improved yield of soluble capsid protein. This is achieved by combining the advantage s of fed-batch culture and of lactose induced expression systems with specific process parameters providing improved repression of the promoter during the growth phase and high plasmid retention throughout the process.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式