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1. 发明申请

WO2010088456A3 SWELLABLE (METH)ACRYLATE SURFACES FOR CULTURING CELLS IN CHEMICALLY DEFINED MEDIA 审中-公开
标题翻译：用于化学定义培养基培养细胞的可裂解（METH）丙烯酸酯表面
公开(公告)号：WO2010088456A3
公开(公告)日：2011-02-24
申请号：PCT/US2010022497
申请日：2010-01-29
申请人： CORNING INC , FADEEV ANDREI , GEHMAN JENNIFER , MARTIN ARTHUR W , MELKOUMIAN ZARA , SHOGBON CHRISTOPHER B , WEBER DAVID M , ZHOU YUE
发明人： FADEEV ANDREI , GEHMAN JENNIFER , MARTIN ARTHUR W , MELKOUMIAN ZARA , SHOGBON CHRISTOPHER B , WEBER DAVID M , ZHOU YUE
IPC分类号： C12N5/00
CPC分类号： C12N5/0606 , C12N5/0068 , C12N2533/30 , C12N2533/50
摘要： Synthetic surfaces capable of supporting culture of undifferentiated human embryonic stem cells in a chemically defined medium include a swellable (meth)acrylate layer and a peptide conjugated to the swellable (meth)acrylate layer. The swellable (meth)acrylate layer may be formed by polymerizing monomers in a composition that includes hydroxyethyl methacrylate, 2-carboxyehylacrylate, and tetra(ethylene glycol) dimethacrylate. The conjugated peptide may include an amino acid sequence of XaanProGlnValThrArgGlyAspValPheThrMetPro, where n is an integer from 0 to 3 and where Xaa is any amino acid. Further, disclosed herein is a swellable (meth)acrylate synthetic surface which can be sterilized by gamma irradiation.
摘要翻译：能够支持未分化的人胚胎干细胞在化学成分确定的培养基中培养的合成表面包括可溶胀的（甲基）丙烯酸酯层和与可溶胀的（甲基）丙烯酸酯层共轭的肽。可溶胀的（甲基）丙烯酸酯层可以通过使包含甲基丙烯酸羟乙酯，2-羧基丙烯酸烯丙酯和四（乙二醇）二甲基丙烯酸酯的组合物中的单体聚合而形成。缀合肽可以包括XananProGlnValThrArgGlyAspValPheThrMetPro的氨基酸序列，其中n是0-3的整数，并且Xaa是任何氨基酸。此外，本文公开了可通过γ辐射灭菌的可溶胀（甲基）丙烯酸酯合成表面。

2. 发明申请

WO2009099552A3 CELL CULTURE ARTICLE AND SCREENING 审中-公开
标题翻译：细胞培养文章和筛选
公开(公告)号：WO2009099552A3
公开(公告)日：2010-01-14
申请号：PCT/US2009000589
申请日：2009-01-30
申请人： CORNING INC , GEHMAN JENNIFER , MARTIN ARTHUR W , MELKOUMIAN ZARA , SHOGBON CHRISTOPHER B , WEBER DAVID M , ZHOU YUE
发明人： GEHMAN JENNIFER , MARTIN ARTHUR W , MELKOUMIAN ZARA , SHOGBON CHRISTOPHER B , WEBER DAVID M , ZHOU YUE
IPC分类号： C12N5/00
CPC分类号： C12N5/0068 , C12N5/0606 , C12N2533/30 , C12N2533/50 , C12N2537/10
摘要： A method for producing a cell culture article having a synthetic polymer layer for incubating with cells includes diluting one or more (meth)acrylate monomers in a solvent and dispersing the diluted monomers on a surface of the cell culture article. Some or substantially all of the solvent is removed and the monomers are then polymerized on the surface of the article to form the synthetic polymer layer attached to the surface of the article.
摘要翻译：制备具有用于与细胞一起孵育的合成聚合物层的细胞培养物的方法包括在溶剂中稀释一种或多种（甲基）丙烯酸酯单体，并将稀释的单体分散在细胞培养物品的表面上。去除一些或基本上所有的溶剂，然后在制品的表面上聚合单体以形成附着到制品表面的合成聚合物层。

3. 发明申请

WO2009099539A2 (METH)ACRYLATE SURFACES FOR CELL CULTURE, METHODS OF MAKING AND USING THE SURFACES 审中-公开
标题翻译：（METH）丙烯酸酯表面细胞培养，制备和使用表面的方法
公开(公告)号：WO2009099539A2
公开(公告)日：2009-08-13
申请号：PCT/US2009000545
申请日：2009-01-28
申请人： CORNING INC , MARTIN ARTHUR W , MELKOUMIAN ZARA , SHOGBON CHRISTOPHER B , ZHOU YUE
发明人： MARTIN ARTHUR W , MELKOUMIAN ZARA , SHOGBON CHRISTOPHER B , ZHOU YUE
IPC分类号： C08F126/02 , C08F20/10 , C08F26/06 , C08F120/56 , C08F220/26 , C08F224/00 , C08F226/10 , C08J7/18 , C12N5/00
CPC分类号： C12N5/0068 , C08J7/047 , C08J2433/00 , C12N2533/30
摘要： A synthetic cell culture surface, prepared from a polymerized blend of at least two (meth)acrylate monomers is provided, which supports the growth of undifferentiated human embryonic stem cells in defined media augmented with fetal bovine serum. The cell culture surface forms a uniform layer over the growth area of a typical cell culture vessel.
摘要翻译：提供了由至少两种（甲基）丙烯酸酯单体的聚合共混物制备的合成细胞培养物表面，其支持未分化的人胚胎干细胞在用胎牛血清增强的限定培养基中的生长。细胞培养表面在典型的细胞培养容器的生长区域上形成均匀的层。

4. 发明申请

WO2011106222A3 MODIFIED SUBSTRATES FOR PROTECTION OF PEPTIDE-IMMOBILIZED SURFACES FROM GAMMA RADIATION DEGRADATION 审中-公开
标题翻译：用于保护γ-辐射降解的肽 - 固定化表面的修饰的基质
公开(公告)号：WO2011106222A3
公开(公告)日：2011-11-24
申请号：PCT/US2011025138
申请日：2011-02-17
申请人： CORNING INC , BOOKBINDER DANA C , MARTIN ARTHUR W , PAL SANTONA , SHOGBON CHRISTOPHER B , WEBER JENNIFER , ZHOU YUE
发明人： BOOKBINDER DANA C , MARTIN ARTHUR W , PAL SANTONA , SHOGBON CHRISTOPHER B , WEBER JENNIFER , ZHOU YUE
IPC分类号： C12N5/00
CPC分类号： C12N5/0068 , C12N2533/20 , C12N2533/50
摘要： In the present specification and claims, three modified cell culture substrates are disclosed for protecting peptide mimetic surfaces used in cell culture technology. The methods are able to retain the functionality of the bioactive species conjugated to the surface. In particular, a vitronectin peptide fragment Ac-Lys-Gly-Gly-Pro-Gln-Val-Thr-Arg-Gly-Asp-Val-Phe-Thr-Met-Pro-NH2 was able to facilitate growth and proliferation of undifferentiated human embryonic stem cells after stabilization using 3 different modified cell culture substrates for of protection against gamma irradiation. The modified substrates disclosed in this invention are (i) covalent attachment of the antioxidant molecules (via blocking step); (ii) The use of coatings consisting of antioxidants and stabilizers comprising glycoprotiens, sugars, carbohydrates, poly(amino acids), peptides and hydrophilic polymers; and (iii) a third method wherein the same methionine contained in the bioactive sequence used to facilitate growth and proliferation of human embryonic stem cells was also used as a sacrificial coating to protect the same conjugated sequence against damage by gamma irradiation.
摘要翻译：在本说明书和权利要求书中，公开了三种修饰的细胞培养基质，用于保护细胞培养技术中使用的肽模拟物表面。该方法能够保持与表面结合的生物活性物质的功能。具体而言，玻连蛋白肽片段Ac-Lys-Gly-Gly-Pro-Gln-Val-Thr-Arg-Gly-Asp-Val-Phe-Thr-Met-Pro-NH 2能够促进未分化人的生长和增殖胚胎干细胞稳定后使用3种不同的修饰细胞培养基质来防止γ辐射。本发明公开的改性底物是（i）抗氧化剂分子的共价连接（通过封闭步骤）; （ii）使用由抗氧化剂和稳定剂组成的包衣，所述稳定剂包含糖蛋白，糖，碳水化合物，聚（氨基酸），肽和亲水性聚合物; 和（iii）第三种方法，其中用于促进人类胚胎干细胞生长和增殖的生物活性序列中所含的相同甲硫氨酸也用作牺牲性涂层以保护相同的缀合序列免受伽玛辐射的损害。

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