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    • 4. 发明申请
    • METHOD FOR STERILIZING MICROBIAL CELLS USING POLYETHYLENE GLYCOL-BASED NONIONIC SURFACTANT
    • 利用聚乙二醇基非离子表面活性剂灭菌微生物细胞的方法
    • WO2013095002A3
    • 2013-08-15
    • PCT/KR2012011161
    • 2012-12-20
    • CJ CHEILJEDANG CORP
    • KIM JIN HALEE YOUNG MIKIM SEONG BOKIM TAEK BEOMKIM YANG HEEPARK SEUNG WON
    • A23L29/00C12N1/36C12Q1/02C12Q1/22
    • A23L29/06A23L3/3508A23L29/065C12N1/20C12N9/90C12N11/16C12P19/02C12P19/24C12Y503/01003Y02P20/52
    • The present invention relates to a method for sterilizing microbial cells and to sterilized microbial cells, in which microbial cells or a culture containing microbial cells is treated with a polyethylene glycol-based nonionic surfactant so that almost all of the microbial cells are sterilized while the enzyme activity expressed in the microbial cells is maintained at a high level. In detail, the present invention relates to a method for sterilizing microbial cells and a material containing the sterilized microbial cells, in which the microbial cells are sterilized using a polyethylene glycol-based nonionic surfactant that can be used for foods so that the microbial cells are sterilized to be used in food production. Further, the present invention relates to a material containing sterilized microbial cells that can be used in processes for preparing tagatose, in which Corynebacterium genus microbial cells that produce Galactose and/or Arabinose isomerase are sterilized using a polyethylene glycol-based nonionic surfactant.
    • 本发明涉及杀菌微生物细胞和杀菌微生物细胞的方法,其中用聚乙二醇基非离子表面活性剂处理微生物细胞或含有微生物细胞的培养物,使得几乎所有的微生物细胞都被灭菌,而酶 在微生物细胞中表达的活性保持在高水平。 详细而言,本发明涉及杀菌微生物细胞的方法和含有杀菌微生物细胞的材料,其中使用可用于食品的基于聚乙二醇的非离子表面活性剂对微生物细胞进行灭菌,使得微生物细胞为 消毒用于食品生产。 此外,本发明涉及可用于制备塔格糖的方法中的灭菌微生物细胞的材料,其中使用基于聚乙二醇的非离子表面活性剂对产生半乳糖和/或阿拉伯糖异构酶的棒状杆菌属微生物细胞进行灭菌。