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1. 发明授权

US10900885B2 Flow cytometry using hydrodynamically planar flow 有权
公开(公告)号：US10900885B2
公开(公告)日：2021-01-26
申请号：US15537744
申请日：2015-12-19
申请人： CAPTL LLC , Hamamatsu Photonics K.K.
发明人： Masanobu Yamamoto , J. Paul Robinson , Yuji Masuda , Tsukasa Kayou
IPC分类号： G01N15/14 , B01L3/00
摘要： According to various aspects, a flow system for transporting microparticulate samples in a hydrodynamically planar flow in a selected flow direction includes a flow chamber extending in the flow direction, having first and second apertures on opposed surfaces of the flow chamber. A sheath-fluid channel has first and second branches to carry the sheath fluid into the flow chamber through the first aperture and having orientations separated by less than about 15° at the first aperture; and third and fourth branches to carry the sheath fluid through the second aperture and having orientations separated by less than about 15° at the second aperture. In some examples, guide channels extend from the apertures substantially perpendicular to the flow chamber at the apertures, and sheath-fluid channel supply sheath fluid to the guide channels. Flow systems can be used in image flow cytometers for observing microparticulate samples, e.g., using scanning irradiation.

2. 发明申请

US20190049356A1 Multiple-Spot Time-Sequential Cytometry 审中-公开
公开(公告)号：US20190049356A1
公开(公告)日：2019-02-14
申请号：US16045094
申请日：2018-07-25
申请人： CAPTL LLC
发明人： Masanobu Yamamoto , J. Paul Robinson
IPC分类号： G01N15/14 , G01N15/10
CPC分类号： G01N15/1427 , G01N15/1404 , G01N15/1434 , G01N15/147 , G01N15/1475 , G01N2015/1006 , G01N2015/1409 , G01N2015/1413 , G01N2015/144 , G01N2015/1452
摘要： An image flow cytometer for observing a microparticulate sample includes a flow chamber having a flow channel that permits the microparticulate sample to travel in a flow direction. An irradiation system scans an irradiation spot across a sensing area of the flow channel in a scan direction different from the flow direction. A detection system detects resultant light from the sensing area and provides a detection signal. An alignment system alters a location of the sensing area with respect to the flow chamber. A control unit causes the irradiation system to scan the irradiation spot during a first measurement interval and operates the alignment system to translate the location of the sensing area along the flow direction. The flow chamber can be mounted to a movable stage in some examples, and the alignment system can move the flow chamber substantially opposite the flow direction using the stage.

3. 发明申请

US20180038783A1 FLOW CYTOMETRY USING HYDRODYNAMICALLY PLANAR FLOW 审中-公开
公开(公告)号：US20180038783A1
公开(公告)日：2018-02-08
申请号：US15537744
申请日：2015-12-19
申请人： CAPTL LLC , Hamamatsu Photonics K.K.
发明人： Masanobu Yamamoto , J. Paul Robinson , Yuji Masuda , Tsukasa Kayou
IPC分类号： G01N15/14 , B01L3/00
CPC分类号： G01N15/1404 , B01L3/502761 , B01L3/502776 , B01L2300/0654 , B01L2300/0816 , B01L2300/0867 , B01L2300/0874 , B01L2300/0877 , B01L2300/0887 , B01L2400/0487 , G01N15/1434 , G01N15/1459 , G01N15/147 , G01N15/1484 , G01N2015/1409 , G01N2015/1413 , G01N2015/144
摘要： According to various aspects, a flow system for transporting microparticulate samples in a hydrodynamically planar flow in a selected flow direction includes a flow chamber extending in the flow direction, having first and second apertures on opposed surfaces of the flow chamber. A sheath-fluid channel has first and second branches to carry the sheath fluid into the flow chamber through the first aperture and having orientations separated by less than about 15° at the first aperture; and third and fourth branches to carry the sheath fluid through the second aperture and having orientations separated by less than about 15° at the second aperture. In some examples, guide channels extend from the apertures substantially perpendicular to the flow chamber at the apertures, and sheath-fluid channel supply sheath fluid to the guide channels. Flow systems can be used in image flow cytometers for observing microparticulate samples, e.g., using scanning irradiation.

4. 发明授权

US10613096B2 Multi-spectral microparticle-fluorescence photon cytometry 有权
公开(公告)号：US10613096B2
公开(公告)日：2020-04-07
申请号：US15250136
申请日：2016-08-29
申请人： CAPTL LLC
发明人： Masanobu Yamamoto , J. Paul Robinson
IPC分类号： G01N33/58 , B01L3/00 , G01N35/00 , G01N15/14 , G01N21/64
CPC分类号： G01N33/582 , B01L3/00 , G01N15/1434 , G01N35/00069 , G01N15/1456 , G01N15/1459 , G01N15/1484 , G01N21/645 , G01N2035/00881 , G01N2201/1045
摘要： A measurement system includes a system for causing relative motion between a sample and an irradiation spot. The sample includes fluorescent markers having respective wavelengths. A gating system provides a gating signal based at least in part on resultant light substantially at an irradiation wavelength. A detection system detects fluorescent light from the irradiated markers and provides detection signals representing the fluorescent light detected concurrently with a gate-open signal. In some examples, the detection system detects fluorescent light at multiple wavelengths and provides respective detection signals. A spectral discriminator arranged optically between the sample and the detection system receives the fluorescent light from the sample and provides respective fluorescent light at the wavelengths to the detection system. A flow cytometer can spectrally disperse resultant fluorescent light and measure the wavelengths separately. Light from a sample disposed over a reflective phase grating can be dispersed, measured, and gated.

5. 发明申请

US20160370280A1 TIME-SEQUENTIAL CYTOMETRY 审中-公开
标题翻译：时间序列测定
公开(公告)号：US20160370280A1
公开(公告)日：2016-12-22
申请号：US15187346
申请日：2016-06-20
申请人： CAPTL LLC
发明人： Masanobu Yamamoto , J. Paul Robinson
IPC分类号： G01N15/14
CPC分类号： G01N15/1427 , G01N15/1404 , G01N15/1434 , G01N15/147 , G01N15/1475 , G01N2015/1006 , G01N2015/1409 , G01N2015/1413 , G01N2015/144 , G01N2015/1452
摘要： An image flow cytometer for observing a microparticulate sample includes a flow chamber having a flow channel that permits the microparticulate sample to travel in a flow direction. An irradiation system scans an irradiation spot across a sensing area of the flow channel in a scan direction different from the flow direction. A detection system detects resultant light from the sensing area and provides a detection signal. An alignment system alters a location of the sensing area with respect to the flow chamber. A control unit causes the irradiation system to scan the irradiation spot during a first measurement interval and operates the alignment system to translate the location of the sensing area along the flow direction. The flow chamber can be mounted to a movable stage in some examples, and the alignment system can move the flow chamber substantially opposite the flow direction using the stage.
摘要翻译：用于观察微粒样品的图像流式细胞仪包括具有允许微粒样品沿流动方向行进的流动通道的流动室。照射系统沿着与流动方向不同的扫描方向扫描流路的感测区域上的照射点。检测系统检测来自感测区域的合成光并提供检测信号。对准系统相对于流动室改变感测区域的位置。控制单元使得照射系统在第一测量间隔期间扫描照射点，并且操作对准系统以沿着流动方向平移感测区域的位置。在一些示例中，流动室可以安装到可移动台，并且对准系统可以使用该台基本上与流动方向相反地移动流动室。

6. 发明授权

US09372143B2 Scanning image flow cytometer 有权
标题翻译：扫描图像流式细胞仪
公开(公告)号：US09372143B2
公开(公告)日：2016-06-21
申请号：US13894521
申请日：2013-05-15
申请人： CAPTL LLC
发明人： Masanobu Yamamoto , J. Paul Robinson
IPC分类号： G01N15/14
CPC分类号： G01N15/1434 , G01N15/1429 , G01N15/1459
摘要： An image flow cytometer has a flow chamber with a flow channel formed therein to permit a microparticulate sample to flow through the flow channel. An irradiation optical system irradiates the sample in the channel with incident light in an irradiation spot smaller than a selected representative size, e.g., smaller than the sample. The system scans an irradiation position perpendicular to the flow direction of the sample. A detection optical system is opposed to the irradiation optical system through the flow chamber, or is off the optical axis of the incident light. The detection system detects a light intensity of resultant light from the flow chamber. A control unit detects the microparticulate sample according to a change of the light intensity of the resultant light detected by the detection optical system.
摘要翻译：图像流式细胞仪具有流动室，其中形成有流动通道，以允许微粒样品流过流动通道。照射光学系统在入射光中照射具有小于所选代表尺寸的照射点的样品，例如小于样品。系统扫描垂直于样品流动方向的照射位置。检测光学系统与通过流动室的照射光学系统相对，或者离开入射光的光轴。检测系统检测来自流动室的合成光的光强度。控制单元根据由检测光学系统检测到的所得光的光强度的变化来检测微粒样品。

7. 发明授权

US11187584B2 Photon counting and spectroscopy 有权
公开(公告)号：US11187584B2
公开(公告)日：2021-11-30
申请号：US16604557
申请日：2018-04-12
申请人： CAPTL LLC
发明人： Masanobu Yamamoto , Keegan Hernandez , J. Paul Robinson
IPC分类号： G01J3/44 , G01J3/02
摘要： A measurement system includes an optical source (e.g., laser) to irradiate a sample (e.g., a cell); a solid-state photon detector (SSPD) to receive resultant light from the sample; and a photon counter to count photons received by the SSPD. The photon counter can include a differentiator to provide a differentiated photon signal and a crossing detector configured to count photons based on a number of times the differentiated photon signal crosses a predetermined threshold level. In some examples, a pulse detector can provide a pulse-width signal from the SSPD output photon signal, and a pulse counter can count based on both a number of pulses and widths of the pulses. The SSPD can include a silicon photomultiplier (SiPM) array or a solid-state photomultiplier. Some examples use the measurement system to measure samples in fluids, e.g., in flow cytometers or multi-well plates.

8. 发明申请

US20200340999A1 MULTI-SPECTRAL MICROPARTICLE-FLUORESCENCE PHOTON CYTOMETRY 审中-公开
公开(公告)号：US20200340999A1
公开(公告)日：2020-10-29
申请号：US16840220
申请日：2020-04-03
申请人： CAPTL LLC
发明人： Masanobu Yamamoto , J. Paul Robinson
IPC分类号： G01N33/58 , B01L3/00 , G01N35/00 , G01N15/14
摘要： A measurement system includes a system for causing relative motion between a sample and an irradiation spot. The sample includes fluorescent markers having respective wavelengths. A gating system provides a gating signal based at least in part on resultant light substantially at an irradiation wavelength. A detection system detects fluorescent light from the irradiated markers and provides detection signals representing the fluorescent light detected concurrently with a gate-open signal. In some examples, the detection system detects fluorescent light at multiple wavelengths and provides respective detection signals. A spectral discriminator arranged optically between the sample and the detection system receives the fluorescent light from the sample and provides respective fluorescent light at the wavelengths to the detection system. A flow cytometer can spectrally disperse resultant fluorescent light and measure the wavelengths separately. Light from a sample disposed over a reflective phase grating can be dispersed, measured, and gated.

9. 发明申请

US20180364159A1 Particle Detection Using Reflective Surface 审中-公开
公开(公告)号：US20180364159A1
公开(公告)日：2018-12-20
申请号：US16110285
申请日：2018-08-23
申请人： CAPTL LLC
发明人： Masanobu Yamamoto , J. Paul Robinson
IPC分类号： G01N21/47 , B01L3/00
摘要： An example assembly includes a target holder that retains a target in a detection region. Areflective surface reflects at least part of a focused spot of light to provide resultant light. An irradiation system irradiates at least part of the detection region with the focused spot of light. A motion system causes motion of the focused spot of light relative to the reflective surface. A detection system detects the resultant light. An example device, e.g., a lab-on-chip, includes a substrate, a sample inlet, and a reflective grating. The grating is retains a fluidic sample in a detection region fluidically connected to the sample inlet. The detection region is operatively arranged with respect to the reflective grating so that at least a portion of light passing through the detection region towards the reflective grating also passes through the detection region after reflecting off the reflective grating.

10. 发明申请

US20220057317A1 PHOTON COUNTING AND MULTI-SPOT SPECTROSCOPY 有权
公开(公告)号：US20220057317A1
公开(公告)日：2022-02-24
申请号：US17415311
申请日：2019-12-16
申请人： CAPTL LLC
发明人： Masanobu Yamamoto , J. Paul Robinson , Keegan Hernandez
IPC分类号： G01N15/14 , H01L31/02 , H01L31/107
摘要： An example system can include a support and two or more sensor elements mounted to the support. Each sensor element can be electrically connected to a common electrical node and may include: a respective quench resistor connected to a respective internal node; and a respective photodiode (PD) connected to the respective internal node; a differentiating element fed by at least one of the photodiodes; a first readout electrode fed by the common electrical node; and a second readout electrode fed by the differentiating element. The common electrical node may be connected to at least one of the quench resistors or at least one of the photodiodes.

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