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    • 7. 发明申请
    • BIOMEDICAL AND CHEMICAL SENSING WITH NANOBEAM PHOTONIC CRYSTAL CAVITIES USING OPTICAL BISTABILITY
    • 使用光学稳定性的纳米级光子晶体生物医学和化学传感
    • WO2011133670A2
    • 2011-10-27
    • PCT/US2011/033251
    • 2011-04-20
    • PRESIDENT AND FELLOWS OF HARVARD COLLEGEQUAN, QiminVOLLMER, FrankLONCAR, Marko
    • QUAN, QiminVOLLMER, FrankLONCAR, Marko
    • G01N33/483B82B3/00G01N21/27
    • G01N21/63G01N21/77G01N2021/7789
    • A miniature optical biosensor and biosensor array where high sensitivity for detection of biomolecular interaction does not require a fluorescent label. Non-linear frequency- shifts of optical resonators ('nanobeams') provide a digital all-or-nothing response to equilibrium binding of a biomarker to surface-immobilized bio-recognition elements, a signal suitable to identify active components in genetic and proteomic circuits, as well as toxic substances. The threshold level for the digital response is adjustable to accommodate for varying receptor affinities. A bistable cavity sensing (BCS) method can be used to track the shift of the resonance induced by the analyte more precisely than the conventional cavity sensing method, where the resolution is limited by the cavity linewidth. BCS method can be used to quantitate the concentration of the analyte, and their binding kinetics, affinities and etc.
    • 用于检测生物分子相互作用的高灵敏度的微型光学生物传感器和生物传感器阵列不需要荧光标记。 光学谐振器(“nanobeams”)的非线性频移为生物标志物与表面固定的生物识别元件的平衡结合提供了数字全无反应,适合于鉴定遗传和蛋白质组学电路中的有效成分的信号 ,以及有毒物质。 数字响应的阈值水平是可调节的,以适应不同的受体亲和力。 可以使用双稳态腔感测(BCS)方法跟传统腔感测方法更精确地跟踪由分析物引起的共振的偏移,其中分辨率受空腔线宽的限制。 BCS法可用于定量分析物的浓度及其结合动力学,亲和力等。