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1. 发明授权

US06986992B2 P450 single nucleotide polymorphism biochip analysis 失效
标题翻译： P450单核苷酸多态性生物芯片分析
公开(公告)号：US06986992B2
公开(公告)日：2006-01-17
申请号：US10114908
申请日：2002-04-01
申请人： Buena Chui , Robert Elghanian , Vineet Gupta , Krishnamurthy Jayaraman , Gretchen Kiser , Changming Li , Chang-Gong Liu , Kenneth R. Luehrsen , Abhijit Mazumder , Ramesh Ramakrishnan , Arkadiy Silbergleyt , Todd Tuggle , Carl Yamashiro , Handy Yowanto , Ekaterina Pestova , David R. Fermin , David G. Wang , Zhijie John Gu
发明人： Buena Chui , Robert Elghanian , Vineet Gupta , Krishnamurthy Jayaraman , Gretchen Kiser , Changming Li , Chang-Gong Liu , Kenneth R. Luehrsen , Abhijit Mazumder , Ramesh Ramakrishnan , Arkadiy Silbergleyt , Todd Tuggle , Carl Yamashiro , Handy Yowanto , Ekaterina Pestova , David R. Fermin , David G. Wang , Zhijie John Gu
IPC分类号： C12Q1/68
CPC分类号： B82Y30/00 , C12Q1/6837 , C12Q1/6881 , C12Q1/6883 , C12Q2600/156 , C12Q2600/172 , C12Q2535/125
摘要： This invention relates to methods and compositions for determining single nucleotide polymorphisms (SNPs) in P450 genes. In preferred embodiments, self extension of interrogation probes is prevented by using novel non self-extension probes and/or methods, thereby improving the specificity and efficiency of P450 SNP detection in target samples with minimal false positive results. The invention thus describes a variety of methods to decrease self-extension of interrogation probes. In addition, this invention provides a unique collection of P450 SNP probes on one assay, primer sequences for specific amplification of each of the seven P450 genes and amplicon control probes to evaluate whether the intended p450 gene targets were amplified successfully. The invention also describes a variety of array platforms for performing the assays of the invention; for example: CodeLink™, eSensor™, multiplex arrays with cartridges etc., all described herein.
摘要翻译：本发明涉及用于测定P450基因单核苷酸多态性（SNPs）的方法和组合物。在优选的实施方案中，通过使用新的非自扩增探针和/或方法来阻止询问探针的自身延伸，从而以最小的假阳性结果提高目标样品中P450 SNP检测的特异性和效率。因此，本发明描述了减少询问探针自身延伸的各种方法。此外，本发明在一个测定中提供P450 SNP探针的独特收集，用于七个P450基因和扩增子对照探针中的每一个的特异性扩增的引物序列，以评估是否成功扩增了预期的p450基因靶标。本发明还描述了用于进行本发明测定的各种阵列平台; 例如：CodeLink TM，eSensor TM，带有墨盒的多重阵列等。

2. 发明授权

US06642046B1 Method and apparatus for performing biological reactions on a substrate surface 有权
标题翻译：在基材表面进行生物反应的方法和装置
公开(公告)号：US06642046B1
公开(公告)日：2003-11-04
申请号：US09458534
申请日：1999-12-09
申请人： Mark W. McGarry , Peter Kahn , Todd Tuggle , George Hawkins
发明人： Mark W. McGarry , Peter Kahn , Todd Tuggle , George Hawkins
IPC分类号： C12M134
CPC分类号： B01L7/52 , B01J19/0093 , B01J2219/00317 , B01J2219/00351 , B01J2219/00495 , C40B60/14 , F28F2260/02 , G01N30/6069 , G01N30/6095 , G01N2030/025 , G01N2035/00158 , G01N2035/00237
摘要： The present invention provides methods and apparatus for performing biological reactions on a substrate surface that use a low volume of sample fluid, accommodate substrates as large as or larger than a conventional microscope slide, accommodate a plurality of independent reactions, and accommodate a substrate surface having one or more hydrogel-based microarrays attached thereto. The invention further provides an apparatus that allows introduction of fluids in addition to sample fluid into each reaction chamber via standard pipet tips and associated pipettor apparatus. The invention further an apparatus that increases reaction reproducibility, increases reaction efficiency, and reduces reaction duration. The preferred embodiment of the invention is configured to accommodate a standard microscope slide substrate having four hydrogel-based microarrays attached thereto and comprises a base plate having a well structure corresponding to each microarray and two fluid ports extending through the base plate into each well structure, a means for temporarily clamping the substrate against the base plate such that the microarrays face into the well structures, a means for sealing the perimeter around each microarray and well structure, and a means for sealing the fluid ports from the environment.
摘要翻译：本发明提供用于在使用少量样品流体的衬底表面上进行生物反应的方法和装置，适应与常规显微镜载片一样大或大于其的基底，适应多个独立的反应，并容纳具有一个或多个附着于其上的基于水凝胶的微阵列。本发明还提供了一种装置，其允许除了样品流体之外的流体通过标准吸头和相关联的移液器装置引入每个反应室。本发明还提供一种提高反应重现性，提高反应效率和降低反应持续时间的装置。本发明的优选实施方案被配置为容纳具有附着于其上的四个基于水凝胶的微阵列的标准显微镜载片基底，并且包括具有孔结构对应于每个微阵列和两个通过基板延伸到每个井结构中的流体端口，用于将基板临时夹持在基板上以使微阵列面向井结构的装置，用于密封围绕每个微阵列的周边的装置，井结构以及用于将流体端口与环境密封的装置。

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