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1. 发明授权

US09447371B2 Holder for culture plates with de-nesting feature 有权
标题翻译：用于具有去嵌套特征的培养皿的持有人
公开(公告)号：US09447371B2
公开(公告)日：2016-09-20
申请号：US12221755
申请日：2008-08-05
申请人： Bruno Colin , Yaneli Iakimova , Kevin G. Ketsenburg , David M. Robbins , Stéphane Bellet
发明人： Bruno Colin , Yaneli Iakimova , Kevin G. Ketsenburg , David M. Robbins , Stéphane Bellet
IPC分类号： C12M3/00 , C12M1/22 , G01N35/04
CPC分类号： C12M23/48 , C12M23/10 , G01N35/04 , G01N2035/042 , G01N2035/0425
摘要： A holding structure for holding a plurality of culture plates in a stacked arrangement includes a feature for de-nesting the bottom culture plate from the plate above it. The de-nesting feature is preferably a structure which urges a plate to move laterally relative to the plate above it. The de-nesting of the bottom culture plate from the adjacent culture plate facilitates withdrawal of the bottom culture plate from the base via a robotic plate transfer mechanism. The de-nesting feature can be entirely passive, such as ramps formed in the base of the holding structure.
摘要翻译：用于保持多个培养板的堆叠布置的保持结构包括用于将底部培养板从其上方的板上嵌套的特征。去嵌套特征优选地是一种促使板相对于其上方的板横向移动的结构。来自相邻培养板的底部培养板的解嵌有助于通过机器人板转移机构将底部培养板从基底中取出。去嵌套特征可以是完全无源的，例如形成在保持结构的基部中的斜面。

2. 发明申请

US20100035338A1 Holder for culture plates with de-nesting feature 有权
标题翻译：用于具有去嵌套特征的培养皿的持有人
公开(公告)号：US20100035338A1
公开(公告)日：2010-02-11
申请号：US12221755
申请日：2008-08-05
申请人： Bruno Colin , Yaneli Iakimova , Kevin G. Ketsenburg , David M. Robbins , Stephane Bellet
发明人： Bruno Colin , Yaneli Iakimova , Kevin G. Ketsenburg , David M. Robbins , Stephane Bellet
IPC分类号： C12M1/00 , B65G61/00
CPC分类号： C12M23/48 , C12M23/10 , G01N35/04 , G01N2035/042 , G01N2035/0425
摘要： A holding structure for holding a plurality of culture plates in a stacked arrangement includes a feature for de-nesting the bottom culture plate from the plate above it. The de-nesting feature is preferably a structure which urges a plate to move laterally relative to the plate above it. The de-nesting of the bottom culture plate from the adjacent culture plate facilitates withdrawal of the bottom culture plate from the base via a robotic plate transfer mechanism. The de-nesting feature can be entirely passive, such as ramps formed in the base of the holding structure.
摘要翻译：用于保持多个培养板的堆叠布置的保持结构包括用于将底部培养板从其上方的板上嵌套的特征。去嵌套特征优选地是一种促使板相对于其上方的板横向移动的结构。来自相邻培养板的底部培养板的解嵌有助于通过机器人板转移机构将底部培养板从基底中取出。去嵌套特征可以是完全无源的，例如形成在保持结构的基部中的斜面。

3. 发明申请

US20130004287A1 Holder for Culture Plates with De-Nesting Feature 审中-公开
标题翻译：具有脱巢功能的文化馆持有人
公开(公告)号：US20130004287A1
公开(公告)日：2013-01-03
申请号：US13609382
申请日：2012-09-11
申请人： Bruno Colin , Yaneli lakimova , Kevin G. Ketsenberg , David M. Robbins , Stéphane Bellet
发明人： Bruno Colin , Yaneli lakimova , Kevin G. Ketsenberg , David M. Robbins , Stéphane Bellet
IPC分类号： B65G59/06 , B65G61/00
CPC分类号： C12M23/48 , C12M23/10 , G01N35/04 , G01N2035/042 , G01N2035/0425
摘要： A holding structure for holding a plurality of culture plates in a stacked arrangement includes a feature for de-nesting the bottom culture plate from the plate above it. The de-nesting feature is preferably a structure which urges a plate to move laterally relative to the plate above it. The de-nesting of the bottom culture plate from the adjacent culture plate facilitates withdrawal of the bottom culture plate from the base via a robotic plate transfer mechanism. The de-nesting feature can be entirely passive, such as ramps formed in the base of the holding structure.
摘要翻译：用于保持多个培养板的堆叠布置的保持结构包括用于将底部培养板从其上方的板上嵌套的特征。去嵌套特征优选地是一种促使板相对于其上方的板横向移动的结构。来自相邻培养板的底部培养板的解嵌有助于通过机器人板转移机构将底部培养板从基底中取出。去嵌套特征可以是完全无源的，例如形成在保持结构的基部中的斜面。

4. 发明申请

US20130122606A1 METHOD AND DEVICE FOR CHEMICAL AND/OR BIOLOGICAL ANALYSIS 审中-公开
标题翻译：化学和/或生物分析的方法和装置
公开(公告)号：US20130122606A1
公开(公告)日：2013-05-16
申请号：US13810998
申请日：2011-07-28
申请人： Elisabeth Cerrato , Sylvie Cheucle , Bruno Colin , Virginie Helleux , Cecile Paris
发明人： Elisabeth Cerrato , Sylvie Cheucle , Bruno Colin , Virginie Helleux , Cecile Paris
IPC分类号： G01N33/53
CPC分类号： G01N33/5306 , B01F11/0074 , B01L3/0293 , B01L2400/0433 , B01L2400/0487 , G01N2035/1055 , G01N2035/106
摘要： A method of chemical and/or biological analysis including: providing a container delimited by a wall including an inner surface on which there is deposited an active component intended to make possible a chemical and/or biological reaction with a solution disposed in the container; filling the container with a liquid solution containing an analyte which is to be detected; and in applying to the liquid a succession of movements of aspirating/discharging a volume in the container at a suitable frequency to bring about resonance of the liquid so as to deform the free surface thereof and to result in a preferential distribution of the fluid along the inner surface of the wall of the container.
摘要翻译：一种化学和/或生物分析方法，包括：提供由壁限定的容器，所述壁包括内表面，在该内表面上沉积有效成分，以使得与设置在容器中的溶液形成化学和/或生物反应; 用含有待检测分析物的液体溶液填充容器; 并且在液体中以适当的频率向液体中施加吸入/排出容器的一系列运动，以使液体的共振发生变化，从而使流体沿其自由表面发生优先分配容器壁的内表面。

5. 发明申请

US20130109047A1 METHOD OF SAMPLING AND/OR DEPOSITING A SAMPLE OF BIOLOGICAL MATTER AND DEVICE IMPLEMENTING SUCH A METHOD 有权
标题翻译：采样和/或沉积生物学样本的方法和实现方法的装置
公开(公告)号：US20130109047A1
公开(公告)日：2013-05-02
申请号：US13808501
申请日：2011-07-08
申请人： Jean-Philippe Charrier , Bruno Colin , Laurent Drazek , Cécile Paris , Jean-Claude Raymond , Dominique Decaux
发明人： Jean-Philippe Charrier , Bruno Colin , Laurent Drazek , Cécile Paris , Jean-Claude Raymond , Dominique Decaux
IPC分类号： C12Q1/24
CPC分类号： C12Q1/24 , C12M33/04
摘要： The aim of the present invention is a method of sampling all or part of a sample (11) of biological matter (7), which is crude, enriched or cultured through contact with a culture medium (8), such as agar, for example in a Petri dish, using a probe (3) equipped with a terminal end (4), said sampling method comprising the steps of cooling the terminal end (4) of the probe (3), sticking all or part of the sample (11) of biological matter (7) to be sampled through contact, or by applying a pressure exerted by the terminal end (4) onto the sample (11) of biological matter (7), and sampling of all or part of the sample (11) of biological matter (7) so as to separate the sample (11) of biological matter (7) from the culture medium (8), a method of depositing into a container (9) or onto an analysis plate (14) all or part of a sample (11) of biological matter (7) stuck onto a frosted terminal end (4) of a probe, said depositing method including a step of separating the terminal end (4) of the probe (3) from all or part of the sample (11) of biological matter (7), as well as a device (2), a kit and an apparatus (1) implementing these methods.
摘要翻译：本发明的目的是对生物物质（7）的全部或部分样品（11）进行取样的方法，其通过与诸如琼脂的培养基（8）接触而粗制，富集或培养。在陪替氏培养皿中，使用配备有终端（4）的探针（3），所述取样方法包括冷却探针（3）的末端（4）的步骤，将全部或部分样品（11 ），或通过将终端（4）施加的压力施加到生物物质（7）的样品（11）上，并对样品（11）的全部或部分进行采样）将生物物质（7）的样品（11）与培养基（8）分离，将沉积到容器（9）或分析板（14）中的全部或者将生物物质（7）的样品（11）的一部分粘贴到探针的磨砂末端（4）上，所述沉积方法包括将所述末端（4）来自生物物质（7）的全部或部分样品（11）的探针（3）以及实施这些方法的装置（2），试剂盒和装置（1）。

6. 发明申请

US20080152541A1 Integrated Analysis Device Which Can Be Fitted To A Container Housing A Sample To Be Analyzed 有权
标题翻译：综合分析装置可以适用于集装箱住房要分析的样本
公开(公告)号：US20080152541A1
公开(公告)日：2008-06-26
申请号：US11597867
申请日：2005-06-24
申请人： David Mosticone , Pascal Montes , Jean-Claude Raymond , Bruno Colin , Cecile Paris
发明人： David Mosticone , Pascal Montes , Jean-Claude Raymond , Bruno Colin , Cecile Paris
IPC分类号： G01N30/00 , B01J19/00 , G01N35/10
CPC分类号： B01L3/505 , B01L2300/044 , B01L2300/0663 , B01L2300/0672 , B01L2300/0825 , B01L2400/0406 , Y10T436/110833 , Y10T436/112499
摘要： The present invention relates generally to the field of analysis, for example biological analysis. More specifically, the present invention relates to an analysis device (10, 40, 501, 60) designed to be associated with a container (22, 50, 70) containing a sample (28) to be analyzed, said device comprising essentially: a first impervious membrane (12, 42, 52, 62); a second impervious membrane (14, 44, 54, 64) superposed on the first membrane (12, 42, 52, 62); said first and second membranes being fixedly attached over at least a part of their surface, defining a peripheral zone, in order to define an interstitial space forming an internal reaction cavity (16, 46, 56, 66), with no means of fluid communication with the outside of said analysis device (10, 40, 501, 60); at least one analysis means (18, 47, 57, 67), placed inside said internal reaction cavity (16, 46, 56, 66), designed to be placed in contact with the sample (28); at least one means designed to create a way for the sample to enter said internal reaction cavity. The present invention also relates to a container (22, 50, 70) comprising such an analysis device.The present invention also relates to a method of analyzing a sample contained in a container, in which an analysis device according to the invention is used.
摘要翻译：本发明一般涉及分析领域，例如生物分析。更具体地说，本发明涉及一种设计成与容纳待分析样品（28）的容器（22,50,70）相关联的分析装置（10,40,501,60），所述装置基本上包括：第一不透水膜（12,42,52,62）; 叠置在第一膜（12,42,52,62）上的第二不透水膜（14,44,54,64）; 所述第一和第二膜固定地附接在其表面的至少一部分上，限定了周边区域，以便限定形成内部反应腔（16,46,56,66）的间隙空间，没有任何流体连通与所述分析装置的外部（10,40,501,60）; 放置在与所述样品（28）接触的所述内部反应腔（16,46,56,66）内的至少一个分析装置（18,47,57,67）; 至少一种设计用于创建样品进入所述内部反应腔的方式的装置。本发明还涉及包括这种分析装置的容器（22,50,70）。本发明还涉及一种分析包含在容器中的样品的方法，其中使用了根据本发明的分析装置。

7. 发明授权

US07259021B2 Method for using a test card 失效
标题翻译：使用测试卡的方法
公开(公告)号：US07259021B2
公开(公告)日：2007-08-21
申请号：US10220845
申请日：2001-03-06
申请人： Bruno Colin
发明人： Bruno Colin
IPC分类号： G01N1/10
CPC分类号： B01L3/502723 , B01L3/5025 , B01L3/502738 , B01L2200/0673 , B01L2300/0816 , B01L2400/0481 , B01L2400/0655 , G01N2035/00148 , Y10T436/2575
摘要： The present invention relates to a method for using a test card (1) comprising at least one fluid circuit, constituting a network of channels (3) and/or of compartments (17), for transferring at least one biological liquid from at least one inlet (2) to at least one outlet (6, 17), and a hydrophobic fluid is injected into the test card, into all or part of the network of said card, prior to the transfer of the biological liquid(s), said hydrophobic fluid filling the capillary spaces formed by the angles of the network of channels and/or of compartments of the card and avoiding the formation of bubbles and/or of dead volumes during the transfer of the biological liquid.The invention is of preferential use in the field of microfluidics applied to biology.
摘要翻译：本发明涉及一种使用测试卡（1）的方法，该测试卡（1）包括构成通道（3）和/或隔间（17）的网络的至少一个流体回路，用于从至少一个入口（2）到至少一个出口（6,17），并且疏水性流体在生物液体转移之前被注入到测试卡中，进入所述卡的全部或部分网络中，所述生物液体疏水性流体填充由通道网络和/或隔室的角度形成的毛细管空间，并避免在生物液体的转移期间形成气泡和/或死体积。本发明在应用于生物学的微流体领域中被优先使用。

8. 发明申请

US20070020720A1 Method for the detection and/or identification of bacteria present in a sample 有权
标题翻译：用于检测和/或鉴定样品中存在的细菌的方法
公开(公告)号：US20070020720A1
公开(公告)日：2007-01-25
申请号：US10551989
申请日：2004-04-02
申请人： Bruno Colin , Vincent Atrache , Josep Mercader Badia
发明人： Bruno Colin , Vincent Atrache , Josep Mercader Badia
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , B01L3/502 , B01L2300/0636 , B01L2300/0663 , B01L2300/0832 , B01L2300/18 , B01L2400/0442 , Y10S436/809
摘要： The present invention relates to a method for detecting and/or identifying bacteria present in a liquid or solid sample, characterized in that: a. the sample that may contain said bacteria is placed in a liquid culture medium, in a first container, b. a second container comprising at least one system for detecting said bacteria is provided, c. a means of transfer between the first container and the second container is provided, d. a temperature T1 is applied inside the second container, then e. a temperature T2 is applied inside the second container, f. the temperature T1 is higher than the temperature T2 such that a defined volume of culture medium is transferred from the first container to the second container, g. the presence or absence of bacteria is determined and/or the bacteria are identified within the detection system.
摘要翻译：本发明涉及一种用于检测和/或识别存在于液体或固体样品中的细菌的方法，其特征在于：a。将含有所述细菌的样品置于第一容器中的液体培养基中，b。提供了包含至少一个用于检测所述细菌的系统的第二容器，c。提供了在第一容器和第二容器之间的转移装置，d。在第二容器内施加温度T 1，在第二容器内施加温度T 2，f。温度T 1高于温度T 2，使得限定体积的培养基从第一容器转移到第二容器，g。确定细菌的存在或不存在和/或在检测系统内识别细菌。

9. 发明授权

US07097623B2 Biological sampling method 有权
公开(公告)号：US07097623B2
公开(公告)日：2006-08-29
申请号：US10640646
申请日：2003-08-14
申请人： Bruno Colin , Cécile Jaravel , Marie Privat
发明人： Bruno Colin , Cécile Jaravel , Marie Privat
IPC分类号： A61B5/00 , B65D81/00 , G01N1/10
CPC分类号： G01N35/1016 , G01N2035/1025 , Y10T436/2575
摘要： A method for drawing a biological sample using a suction-discharge device, such as a manual or automatic pipette, built into an automated device or not. A method for detecting the free surface of a biological sample and/or solid, a sample drawing device such as a pipette and a device for detecting the free surface of a biological sample or a solid are also disclosed. This method includes actuating a means causing a pressure variation or an air flow within the suction-discharge device, positioning the lower free end of the device in such a way so that it becomes flush with a free surface of the biological sample, measuring an overpressure within the suction-discharge device, and sampling a quantity of the sample by suction using the device. The invention finds a preferable application in the field of automatic analyzers.

10. 发明授权

US06660472B1 Lysis method for micro-organisms 有权
标题翻译：微生物裂解法
公开(公告)号：US06660472B1
公开(公告)日：2003-12-09
申请号：US09509006
申请日：2000-07-17
申请人： Lise Santoro , Sophie Lopez , Bruno Colin , Corinne Jay , Cécile Paris , Kathleen Ann Clark Dickey
发明人： Lise Santoro , Sophie Lopez , Bruno Colin , Corinne Jay , Cécile Paris , Kathleen Ann Clark Dickey
IPC分类号： C12Q168
CPC分类号： C12N1/066 , Y10T436/143333
摘要： The invention concerns a lysis method for a biological sample comprising at least one micro-organism of the bacteria type, for releasing a nucleic material of interest belonging to said micro-organism, which consists in: providing in a container a biological sample in liquid medium; providing in said container at least one particulate material, relatively hard, and substantially inert with respect to the nucleic material; submitting the biological sample and particulate material mixture to a movement. The invention is characterised in that in combination, the selected movement is of the vortex type, and satisfies the following conditions: the particulate material consists of beads with diameter between 90 and 150 &mgr;m; and the apparent volume of the beads (Vb) and the volume of the liquid sample (Ve) are linked by the relationship Ve=&agr;. Vb, with &agr; ranging between 1.4 and 10 when the container is tubular in shape, and &agr; is not more than 2.1 when the container is in the shape of a disk; under such conditions, without any addition of reagent and/or additional process, the method consists in releasing directly into the liquid medium the nucleic material in native state and accessible to any reagent in a subsequent process.
摘要翻译：本发明涉及包含至少一种细菌类微生物的生物样品的裂解方法，用于释放属于所述微生物的感兴趣的核酸，其包括：在容器中提供液体培养基中的生物样品 ; 在所述容器中提供相对于所述核材料相对较硬且基本上惰性的至少一种颗粒材料; 将生物样品和颗粒物质混合物提交到运动中。本发明的特征在于，所选择的运动是涡流型的，并且满足以下条件：颗粒材料由直径在90至150μm之间的珠粒组成; 并且珠子（Vb）的表观体积和液体样品（Ve）的体积通过关系Ve =α连接。 Vb，当容器为管状形状时，α范围在1.4和10之间，当容器为盘形时，α不大于2.1; 在这种条件下，没有任何添加试剂和/或另外的方法，该方法包括将天然状态的核酸材料直接释放到液体介质中，并在随后的方法中可用于任何试剂。

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