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1. 发明授权

US08871465B2 Method for identifying bacteria from the Bacillus cereus group 有权
标题翻译：从蜡样芽胞杆菌群鉴定细菌的方法
公开(公告)号：US08871465B2
公开(公告)日：2014-10-28
申请号：US13496422
申请日：2010-09-16
申请人： Marie Cellier , John Mills , David Mosticone , Sylvain Orenga , Antoine Vimont
发明人： Marie Cellier , John Mills , David Mosticone , Sylvain Orenga , Antoine Vimont
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , C12Q1/44
摘要： The present invention relates to a method for identifying bacteria of the Bacillus cereus group, comprising the following steps: (a) providing a sample that may contain bacteria of the Bacillus cereus group, a reaction medium comprising at least one fluorescent phosphatidylcholine phospholipase C (PC-PLC) substrate and an inhibitor of Gram-negative bacteria; (b) inoculating the reaction medium with the sample; (c) incubating the inoculated reaction medium; and (d) identifying the bacteria of the Bacillus cereus group by detecting the PC-PLC substrate hydrolysis reaction, in which the pH of the reaction medium and the time necessary for detecting the PC-PLC substrate hydrolysis reaction are adapted such that said hydrolysis reaction by bacteria of the Bacillus cereus group is detected before hydrolysis of the PC-PLC substrate by any Gram-positive bacteria other than those belonging to the Bacillus cereus group, that may be present in the sample.
摘要翻译：本发明涉及一种用于鉴定蜡状芽孢杆菌群体细菌的方法，包括以下步骤：（a）提供可含有蜡样芽孢杆菌群细菌的样品，包含至少一种荧光磷脂酰胆碱磷脂酶C（PC -PLC）底物和革兰氏阴性菌的抑制剂; （b）用样品接种反应介质; （c）孵育接种的反应介质; 和（d）通过检测PC-PLC底物水解反应来鉴定蜡状芽孢杆菌群的细菌，其中反应介质的pH和检测PC-PLC底物水解反应所需的时间适应于所述水解反应在可能存在于样品中的除了属于蜡状芽孢杆菌属的那些之外的任何革兰氏阳性细菌在PC-PLC底物水解之前检测到蜡状芽孢杆菌的细菌。

2. 发明申请

US20110129871A1 CULTURE MEDIUM ENABLING STAPHYLOCOCCUS AUREUS TO BE DIFFERENTIATED FROM COAGULASE-NEGATIVE STAPHYLOCOCCI 审中-公开
标题翻译：培养培养基使用来自糖蛋白阴性噬菌体蛋白的印迹石蜡
公开(公告)号：US20110129871A1
公开(公告)日：2011-06-02
申请号：US13056208
申请日：2009-08-13
申请人： David Mosticone , Sylvain Orenga , Antoine Vimont , Yuping Guo , Martine Dhedin
发明人： David Mosticone , Sylvain Orenga , Antoine Vimont , Yuping Guo , Martine Dhedin
IPC分类号： C12Q1/14 , C07F9/117 , C07F9/655 , C07F9/572 , C12N1/20 , C12Q1/02
CPC分类号： C12Q1/14 , C12N1/20
摘要： A specific culture medium for growth, detection, identification and/or counting of Staphylococcus aureus bacteria, said medium includes at least one fluorogenic, chromogenic or luminescent substrate of phospholipase C. Said medium permits differentiation between Staphylococcus aureus and coagulase-negative staphylococci.
摘要翻译：用于生长，检测，鉴定和/或计数金黄色葡萄球菌的特定培养基，所述培养基包括至少一种磷脂酶C的荧光，显色或发光底物。所述培养基允许在金黄色葡萄球菌和凝固酶阴性葡萄球菌之间进行分化。

3. 发明申请

US20080152541A1 Integrated Analysis Device Which Can Be Fitted To A Container Housing A Sample To Be Analyzed 有权
标题翻译：综合分析装置可以适用于集装箱住房要分析的样本
公开(公告)号：US20080152541A1
公开(公告)日：2008-06-26
申请号：US11597867
申请日：2005-06-24
申请人： David Mosticone , Pascal Montes , Jean-Claude Raymond , Bruno Colin , Cecile Paris
发明人： David Mosticone , Pascal Montes , Jean-Claude Raymond , Bruno Colin , Cecile Paris
IPC分类号： G01N30/00 , B01J19/00 , G01N35/10
CPC分类号： B01L3/505 , B01L2300/044 , B01L2300/0663 , B01L2300/0672 , B01L2300/0825 , B01L2400/0406 , Y10T436/110833 , Y10T436/112499
摘要： The present invention relates generally to the field of analysis, for example biological analysis. More specifically, the present invention relates to an analysis device (10, 40, 501, 60) designed to be associated with a container (22, 50, 70) containing a sample (28) to be analyzed, said device comprising essentially: a first impervious membrane (12, 42, 52, 62); a second impervious membrane (14, 44, 54, 64) superposed on the first membrane (12, 42, 52, 62); said first and second membranes being fixedly attached over at least a part of their surface, defining a peripheral zone, in order to define an interstitial space forming an internal reaction cavity (16, 46, 56, 66), with no means of fluid communication with the outside of said analysis device (10, 40, 501, 60); at least one analysis means (18, 47, 57, 67), placed inside said internal reaction cavity (16, 46, 56, 66), designed to be placed in contact with the sample (28); at least one means designed to create a way for the sample to enter said internal reaction cavity. The present invention also relates to a container (22, 50, 70) comprising such an analysis device.The present invention also relates to a method of analyzing a sample contained in a container, in which an analysis device according to the invention is used.
摘要翻译：本发明一般涉及分析领域，例如生物分析。更具体地说，本发明涉及一种设计成与容纳待分析样品（28）的容器（22,50,70）相关联的分析装置（10,40,501,60），所述装置基本上包括：第一不透水膜（12,42,52,62）; 叠置在第一膜（12,42,52,62）上的第二不透水膜（14,44,54,64）; 所述第一和第二膜固定地附接在其表面的至少一部分上，限定了周边区域，以便限定形成内部反应腔（16,46,56,66）的间隙空间，没有任何流体连通与所述分析装置的外部（10,40,501,60）; 放置在与所述样品（28）接触的所述内部反应腔（16,46,56,66）内的至少一个分析装置（18,47,57,67）; 至少一种设计用于创建样品进入所述内部反应腔的方式的装置。本发明还涉及包括这种分析装置的容器（22,50,70）。本发明还涉及一种分析包含在容器中的样品的方法，其中使用了根据本发明的分析装置。

4. 发明授权

US09322048B2 Kit for identifying bacteria from the Bacillus cereus group 有权
标题翻译：用于鉴定蜡状芽孢杆菌组细菌的试剂盒
公开(公告)号：US09322048B2
公开(公告)日：2016-04-26
申请号：US14494847
申请日：2014-09-24
申请人： Marie Cellier , John Mills , David Mosticone , Sylvain Orenga , Antoine Vimont
发明人： Marie Cellier , John Mills , David Mosticone , Sylvain Orenga , Antoine Vimont
IPC分类号： C12Q1/04 , C12Q1/44
CPC分类号： C12Q1/44 , C12Q1/04 , G01N2333/32
摘要： The present invention relates to the field of microbiological testing of food. It relates to a kit comprising a reaction medium containing at least one inhibitor of Gram-negative bacteria and a fluorescent substrate specific for PC-PLC. It further relates to a diagnostic kit for identifying bacteria of the Bacillus cereus group comprising a container of a selective or nonselective reaction medium with a pH between 6.8 and 8.0, the medium comprising at least one inhibitor of Gram-negative bacteria and a fluorescent phosphatidylcholine phospholipase C (PC-PLC) substrate.
摘要翻译：本发明涉及食品微生物检测领域。它涉及一种试剂盒，其包含含有至少一种革兰氏阴性细菌抑制剂和PC-PLC特异性荧光底物的反应介质。本发明进一步涉及用于鉴定蜡状芽孢杆菌群的细菌的诊断试剂盒，其包括pH在6.8和8.0之间的选择性或非选择性反应介质的容器，所述培养基包含至少一种革兰氏阴性细菌抑制剂和荧光磷脂酰胆碱磷脂酶 C（PC-PLC）底物。

5. 发明申请

US20150176052A1 Method for Identifying Bacteria from the Bacillus Cereus Group 有权
标题翻译：鉴定芽孢杆菌属细菌的方法
公开(公告)号：US20150176052A1
公开(公告)日：2015-06-25
申请号：US14494847
申请日：2014-09-24
申请人： Marie Cellier , John Mills , David Mosticone , Sylvain Orenga , Antoine Vimont
发明人： Marie Cellier , John Mills , David Mosticone , Sylvain Orenga , Antoine Vimont
IPC分类号： C12Q1/44
CPC分类号： C12Q1/44 , C12Q1/04 , G01N2333/32
摘要： The present invention relates to the field of microbiological testing of food. It relates to a method for identifying bacteria of the Bacillus cereus group, comprising the steps consisting in: bringing a sample that may contain bacteria of the Bacillus cereus group, a reaction medium comprising at least one fluorescent phosphatidylcholine phospholipase C substrate and an inhibitor of Gram-negative bacteria into contact, in a container; incubating all of the above together; identifying the bacteria of the Bacillus cereus group by detecting the PC-PLC substrate hydrolysis reaction, in which the pH of the reaction medium and the time necessary for detecting the PC-PLC substrate hydrolysis reaction carried out are adapted in such a way that said hydrolysis reaction by the bacteria of the Bacillus cereus group is detected before the hydrolysis of the PC-PLC substrate by the Gram-positive bacteria other than those belonging to the Bacillus cereus group, and potentially present in the sample, is detectable.
摘要翻译：本发明涉及食品微生物检测领域。本发明涉及一种用于鉴定蜡状芽孢杆菌群体的细菌的方法，其包括以下步骤：将可能含有蜡样芽孢杆菌群的细菌的样品，包含至少一种荧光磷脂酰胆碱磷脂酶C底物的反应介质和革兰氏抑制剂 - 阴性细菌接触，在容器中; 将所有上述一起孵化; 通过检测PC-PLC底物水解反应来鉴定蜡样芽孢杆菌群的细菌，其中反应介质的pH和进行PC-PLC底物水解反应检测所需的时间适应于所述水解在PC-PLC底物除了属于蜡状芽孢杆菌属的那些之外的革兰氏阳性细菌水解之前检测到可能存在于样品中的反应是可检测的。

6. 发明申请

US20110020861A1 METHOD FOR THE REAL-TIME DETECTION OF MICROORGANISMS IN A LIQUID CULTURE MEDIUM BY AGGLUTINATION 审中-公开
标题翻译：通过AGGLUTINATION实时检测液体培养基中的微生物的方法
公开(公告)号：US20110020861A1
公开(公告)日：2011-01-27
申请号：US12918159
申请日：2009-03-12
申请人： Bruno Colin , David Mosticone , Jean-Claude Raymond , Thierry Sofia , Antoine Vimont
发明人： Bruno Colin , David Mosticone , Jean-Claude Raymond , Thierry Sofia , Antoine Vimont
IPC分类号： C12Q1/06 , C12Q1/04 , C12M1/34
CPC分类号： G01N33/569 , C12Q1/04 , G01N33/5304
摘要： A method for detecting at least one microorganism that may be present in a sample, comprising the steps of:a) bringing into contact, in a container: a culture medium that enables the growth and/or detection of microorganisms, said sample and a sensitized solid support;b) subjecting the whole to a temperature that promotes the growth and/or detection of microorganisms; andc) observing, in real time, the appearance of an agglutination indicating the presence of the microorganism(s) or confirming said presence when said microorganisms are detected in said culture medium, when step b) has been completed, anda method for detecting and identifying at least one target microorganism that may be present in a sample.
摘要翻译：一种用于检测可能存在于样品中的至少一种微生物的方法，包括以下步骤：a）在容器中接触：使微生物生长和/或检测的培养基，所述样品和敏化的坚实的支持 b）使整体处于促进微生物生长和/或检测的温度; 以及c）当步骤b）已经完成时，实时地观察表明微生物存在的凝集物的出现或者在所述培养基中检测到所述微生物时确认存在的情况，以及检测方法并鉴定可能存在于样品中的至少一种靶微生物。

7. 发明授权

US11092600B2 Method for the real-time detection of microorganisms in a liquid culture medium by agglutination 有权
公开(公告)号：US11092600B2
公开(公告)日：2021-08-17
申请号：US12918159
申请日：2009-03-12
申请人： Bruno Colin , David Mosticone , Jean-Claude Raymond , Thierry Sofia , Antoine Vimont
发明人： Bruno Colin , David Mosticone , Jean-Claude Raymond , Thierry Sofia , Antoine Vimont
IPC分类号： G01N33/569 , C12Q1/04 , G01N33/53
摘要： A method for detecting at least one microorganism that may be present in a sample, comprising the steps of: a) bringing into contact, in a container: a culture medium that enables the growth and/or detection of microorganisms, said sample and a sensitized solid support; b) subjecting the whole to a temperature that promotes the growth and/or detection of microorganisms; and c) observing, in real time, the appearance of an agglutination indicating the presence of the microorganism(s) or confirming said presence when said microorganisms are detected in said culture medium, when step b) has been completed, and a method for detecting and identifying at least one target microorganism that may be present in a sample.

8. 发明申请

US20120171709A1 Method for Identifying Bacteria from the Bacillus Cereus Group 有权
标题翻译：鉴定芽孢杆菌属细菌的方法
公开(公告)号：US20120171709A1
公开(公告)日：2012-07-05
申请号：US13496422
申请日：2010-09-16
申请人： Marie Cellier , John Mills , David Mosticone , Sylvain Orenga , Antoine Vimont
发明人： Marie Cellier , John Mills , David Mosticone , Sylvain Orenga , Antoine Vimont
IPC分类号： G01N21/76 , C12M1/34
CPC分类号： C12Q1/04 , C12Q1/44
摘要： The present invention relates to the field of microbiological testing of food. It relates to a method for identifying bacteria of the Bacillus cereus group, comprising the steps consisting in:bringing a sample that may contain bacteria of the Bacillus cereus group, a reaction medium comprising at least one fluorescent phosphatidylcholine phospholipase C substrate and an inhibitor of Gram-negative bacteria into contact, in a container;incubating all of the above together;identifying the bacteria of the Bacillus cereus group by detecting the PC-PLC substrate hydrolysis reaction,in which the pH of the reaction medium and the time necessary for detecting the PC-PLC substrate hydrolysis reaction carried out are adapted in such a way that said hydrolysis reaction by the bacteria of the Bacillus cereus group is detected before the hydrolysis of the PC-PLC substrate by the Gram-positive bacteria other than those belonging to the Bacillus cereus group, and potentially present in the sample, is detectable.
摘要翻译：本发明涉及食品微生物检测领域。本发明涉及一种用于鉴定蜡状芽孢杆菌群体的细菌的方法，包括以下步骤：使含有蜡样芽孢杆菌群的细菌的样品，包含至少一种荧光磷脂酰胆碱磷脂酶C底物的反应介质和革兰氏抑制剂 - 阴性细菌接触，在容器中; 将所有上述一起孵化; 通过检测PC-PLC底物水解反应来鉴定蜡样芽孢杆菌群的细菌，其中反应介质的pH和进行PC-PLC底物水解反应检测所需的时间适应于所述水解在PC-PLC底物除了属于蜡状芽孢杆菌属的那些之外的革兰氏阳性细菌水解之前检测到可能存在于样品中的反应是可检测的。

9. 发明申请

US20100331211A1 METHOD FOR REAL-TIME DETECTION OF MICROORGANISMS IN A LIQUID CULTURE MEDIUM USING CELLULAR LYSIS 有权
标题翻译：利用细胞分析实时检测液体培养液中微生物的方法
公开(公告)号：US20100331211A1
公开(公告)日：2010-12-30
申请号：US12918195
申请日：2009-03-12
申请人： David Mosticone , Jean-Claude Raymond , Thierry Sofia , Antoine Vimont
发明人： David Mosticone , Jean-Claude Raymond , Thierry Sofia , Antoine Vimont
IPC分类号： C40B30/06 , C12Q1/04 , C12Q1/06
CPC分类号： C12Q1/04 , C12Q1/22 , C12Q2304/00
摘要： The present invention relates to a method for detecting at least one target microorganism that may be present in a sample, comprising the steps of: a) bringing into contact, in a container: said sample, a medium that enables the growth of the target microorganism(s), and a cell population capable of being lysed by the target microorganism(s); b) subjecting the whole to a temperature that promotes the growth of the target microorganism(s); and c) observing, in real time, lysis of the cell population indicating the presence of the target microorganism(s). The present invention also relates to a method for detecting and identifying at least one target microorganism that may be present in a sample.
摘要翻译：本发明涉及一种用于检测可能存在于样品中的至少一种靶微生物的方法，包括以下步骤：a）在容器中接触所述样品，使得能够生长目标微生物的培养基以及能够被靶微生物裂解的细胞群; b）使整体达到促进靶微生物生长的温度; 和c）实时观察表明存在目标微生物的细胞群体的裂解。本发明还涉及用于检测和鉴定可能存在于样品中的至少一种靶微生物的方法。

10. 发明授权

US10907190B2 Process of directly detecting and identifying a microorganism in a biological sample diluted in an enrichment broth 有权
公开(公告)号：US10907190B2
公开(公告)日：2021-02-02
申请号：US13808485
申请日：2011-07-07
申请人： Vincent Atrache , Bruno Colin , Aurelie Lafay , Bouchra Makrouf , Pascal Montes , David Mosticone , Jean-Claude Raymond , Thierry Sofia , Antoine Vimont
发明人： Vincent Atrache , Bruno Colin , Aurelie Lafay , Bouchra Makrouf , Pascal Montes , David Mosticone , Jean-Claude Raymond , Thierry Sofia , Antoine Vimont
IPC分类号： G01N33/569 , C12Q1/04 , G01N33/543 , G01N33/02
摘要： The present invention generally relates to the field of analysis for example biological analysis. More specifically, the present invention relates to a process of detecting at least one microorganism present in a sample placed in a closed container, said method comprising essentially the following steps: a) Place said sample in contact in the container with at least one culture medium and a support capable of capturing the microorganism(s) to be detected, b) Close the container, c) Place the container under conditions capable of allowing the growth of the microorganism(s), d) Detect, inside said closed container, using detection means, the presence of the microorganism(s) fixed onto the capture support.

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