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1. 发明授权

US07112413B2 Heteroaryl substituted benzothiazole dioxetanes 有权
公开(公告)号：US07112413B2
公开(公告)日：2006-09-26
申请号：US10679368
申请日：2003-10-07
申请人： Brooks Edwards , Irena Bronstein , Zhixian Wang
发明人： Brooks Edwards , Irena Bronstein , Zhixian Wang
IPC分类号： G01N33/533 , G01N33/532 , G01N21/76 , C07D277/62
CPC分类号： C07D417/04 , C07D277/62 , C07D493/04 , C07F9/65512 , C07F9/65586 , C09K9/02 , G01N33/582
摘要： Chemiluminescent heteroaryl substituted benzothiazole 1,2-dioxetane compounds capable of producing light energy when decomposed are provided. These chemiluminescent compounds are represented by the general formula: The heteroaryl substituent Y can be, for example, a pyridyl group or a benzothiazolyl group. The heteroaryl substituted benzothiazole compounds are substantially stable at room temperature. Kits including the heteroaryl substituted dioxetane compounds as well as methods for using these compounds for detecting the presence of one or more analytes in a sample are also provided.

2. 发明授权

US06660529B2 Heteroaryl substituted benzothiazole dioxetanes 有权
标题翻译：杂芳基取代的苯并噻唑二氧杂环丁烷
公开(公告)号：US06660529B2
公开(公告)日：2003-12-09
申请号：US09945652
申请日：2001-09-05
申请人： Brooks Edwards , Irena Bronstein , Zhixian Wang
发明人： Brooks Edwards , Irena Bronstein , Zhixian Wang
IPC分类号： G01N2176
CPC分类号： C07D417/04 , C07D277/62 , C07D493/04 , C07F9/65512 , C07F9/65586 , C09K9/02 , G01N33/582
摘要： Chemiluminescent heteroaryl substituted benzothiazole 1,2-dioxetane compounds capable of producing light energy when decomposed are provided. These chemiluminescent compounds are represented by the general formula: The heteroaryl substituent Y can be, for example, a pyridyl group or a benzothiazolyl group. The heteroaryl substituted benzothiazole compounds are substantially stable at room temperature. Kits including the heteroaryl substituted dioxetane compounds as well as methods for using these compounds for detecting the presence of one or more analytes in a sample are also provided.
摘要翻译：提供了能够在分解时产生光能的化学发光的杂芳基取代的苯并噻唑1,2-二氧杂环丁烷化合物。这些化学发光化合物由以下通式表示：杂芳基取代基Y可以是例如吡啶基或苯并噻唑基。杂芳基取代的苯并噻唑化合物在室温下基本稳定。还提供了包括杂芳基取代的二恶丁环化合物的试剂盒以及使用这些化合物检测样品中一种或多种分析物的存在的方法。

3. 发明申请

US20060024762A1 Heteroaryl substituted benzothiazole dioxetanes 审中-公开
公开(公告)号：US20060024762A1
公开(公告)日：2006-02-02
申请号：US11229576
申请日：2005-09-20
申请人： Brooks Edwards , Irena Bronstein , Zhixian Wang
发明人： Brooks Edwards , Irena Bronstein , Zhixian Wang
IPC分类号： G01N33/573
CPC分类号： G01N33/542 , C07D417/14 , C07F9/65583 , C07F9/65586 , G01N33/573
摘要： Chemiluminescent heteroaryl substituted benzothiazole 1,2-dioxetane compounds capable of producing light energy when decomposed are provided. These chemiluminescent compounds are represented by the general formula: The heteroaryl substituent Y can be, for example, a pyridyl group or a benzothiazolyl group. The heteroaryl substituted benzothiazole compounds are substantially stable at room temperature. Kits including the heteroaryl substituted dioxetane compounds as well as methods for using these compounds for detecting the presence of one or more analytes in a sample are also provided.

4. 发明授权

US06451531B1 Dioxetane labeled probes and detection assays employing the same 有权
标题翻译：二氧杂环丁烷标记的探针和使用其的检测测定
公开(公告)号：US06451531B1
公开(公告)日：2002-09-17
申请号：US09340726
申请日：1999-06-29
申请人： Irena Bronstein , Brooks Edwards , Christopher Martin , John Voyta
发明人： Irena Bronstein , Brooks Edwards , Christopher Martin , John Voyta
IPC分类号： C12Q168
CPC分类号： C07H21/00 , C07D321/00 , C12Q1/6816 , C12Q1/686 , Y02P20/55 , C12Q2561/101 , C12Q2565/113
摘要： Probes labeled with 1,2-dioxetane precursors can be employed in a variety of assays. The probes may be nucleic acid, peptide nucleic acid, proteins including enzyme, antibody or antigen, steroid, carbohydrate, drug or non-drug hapten. The probe is provided with a 1,2-dioxetane precursor bound thereto, generally either covalently, or a strong ligand bond. The dioxetane precursor moiety is converted to a bound 1,2-dioxetane by exposure to singlet oxygen. These dioxetane (labels) either spontaneously decompose, or are induced to decompose by an appropriate trigger to release light. The trigger may be a change in pH temperature, or an agent which removes a protective group. Assay formats in which these 1,2-dioxetane labeled probes and referents may be used to include hybridization assays, immuno assays, gel-based assays and Capillary Zone Electrophoresis.
摘要翻译：用1,2-二氧杂环丁烷前体标记的探针可用于多种测定。探针可以是核酸，肽核酸，蛋白质，包括酶，抗体或抗原，类固醇，碳水化合物，药物或非药物半抗原。探针具有与其结合的1,2-二氧杂环丁烷前体，通常共价或强配体键。通过暴露于单线态氧将二氧杂环丁烷前体部分转化为结合的1,2-二氧环乙烷。这些二氧杂环丁烷（标记）会自发分解，或被适当的触发剂诱导分解以释放光。触发可能是pH温度的变化，或者是除去保护基团的试剂。这些1,2-二氧杂环丁烷标记的探针和指示物可用于包括杂交测定，免疫测定，基于凝胶的测定和毛细管区域电泳的测定形式。

5. 发明授权

US06355441B1 Benzothiazole dioxetanes 有权
标题翻译：苯并噻唑二氧杂环丁烷
公开(公告)号：US06355441B1
公开(公告)日：2002-03-12
申请号：US09362047
申请日：1999-07-28
申请人： Brooks Edwards , Irena Bronstein
发明人： Brooks Edwards , Irena Bronstein
IPC分类号： G01N3353
CPC分类号： C07D277/62 , C07D417/04 , C07D493/04 , C07F9/65512 , C07F9/65586 , G01N33/582 , Y10S530/807
摘要： Chemiluminescent 1,2-dioxetane compounds capable of producing light energy when decomposed, substantially stable at room temperature, represented by the formulas I or II:
摘要翻译：化学发光的1,2-二氧杂环丁烷化合物，当分解时能够产生光能，在室温下基本稳定，由式I或II表示：

6. 发明授权

US5869699A 1,2 chemiluminescent dioxetanes of improved performance 失效
公开(公告)号：US5869699A
公开(公告)日：1999-02-09
申请号：US871963
申请日：1997-06-10
申请人： Irena Bronstein , Brooks Edwards , Alison Sparks
发明人： Irena Bronstein , Brooks Edwards , Alison Sparks
IPC分类号： C07D321/00 , C07F9/655 , C07G3/00 , C12Q1/37 , G01N33/58 , C07F9/28 , C07D305/14
CPC分类号： G01N33/582 , C07D321/00 , C07F9/65512 , C07G3/00 , C12Q1/37 , Y02P20/55
摘要： A new class of stable dioxetanes bears a polycyclic stabilizing group and aryloxy moiety, the oxygen atom of which is provided with a protective group which can be removed by an enzymatic or chemical trigger admixed with the dioxetane. The polycyclic stabilizing group is preferably spiroadamantane. The group further bears an alkoxy, aryloxy, aralkyloxy or cycloalkyloxy moiety which is partially or completely substituted with halogens, particularly fluorine and chlorine. Proper selection of electron active groups on the stabilizing moiety, the aryl group and the OR group yields enhanced enzyme kinetics, superior light intensity and excellent detection sensitivity in various assays.

7. 发明授权

US5851771A Chemiluminescent 1,2-dioxetanes 失效
公开(公告)号：US5851771A
公开(公告)日：1998-12-22
申请号：US588810
申请日：1996-01-19
申请人： Irena Bronstein , Brooks Edwards , Alison Sparks
发明人： Irena Bronstein , Brooks Edwards , Alison Sparks
IPC分类号： G01N33/532 , A43B3/00 , C07C43/178 , C07C43/196 , C07C43/215 , C07C43/225 , C07C43/23 , C07C43/315 , C07C45/67 , C07C47/565 , C07C47/575 , C07C65/03 , C07C65/21 , C07C69/00 , C07C69/017 , C07C69/24 , C07C69/78 , C07C69/84 , C07C69/92 , C07C205/06 , C07C205/37 , C07C205/44 , C07C217/84 , C07C233/18 , C07C233/75 , C07C271/28 , C07C271/58 , C07C309/75 , C07C311/04 , C07C311/08 , C07C311/16 , C07D321/00 , C07F7/08 , C07F7/18 , C07F9/12 , C07F9/40 , C07F9/655 , C07F9/6574 , C07G3/00 , C07H15/203 , C07H15/26 , C12Q1/34 , C12Q1/37 , C12Q1/42 , C12Q1/68 , G01N21/76 , G01N21/78 , G01N33/52 , G01N33/535 , G01N33/58
CPC分类号： G01N33/582 , A43B1/0036 , A43B1/0072 , A43B3/0031 , C07C205/37 , C07C205/44 , C07C217/84 , C07C233/18 , C07C271/28 , C07C271/58 , C07C43/1788 , C07C43/196 , C07C43/215 , C07C43/225 , C07C43/23 , C07C43/315 , C07C45/673 , C07C47/565 , C07C47/575 , C07C65/03 , C07C65/21 , C07D321/00 , C07F9/12 , C07F9/4056 , C07F9/65512 , C07F9/65742 , C07G3/00 , C07H15/203 , C07H15/26 , C12Q1/34 , C12Q1/37 , C12Q1/42 , G01N21/76 , G01N33/581 , C07C2103/74 , C12Q2334/00 , Y10S435/81 , Y10S435/975
摘要： Spiroadamantyl dioxetanes bearing an alkoxy substituent, and an aromatic substituent of phenyl or naphthyl on the dioxetane ring can be activated to chemiluminesce if the aromatic substituent bears a moiety designated OX, wherein the X is cleaved by an enzyme with which the dioxetane is permitted to come in contact with. The T.sub.1/2 kinetics of the chemiluminescent reaction, as well as the signal intensity, or quantum yield of the chemiluminescent reaction, can be altered by selection of an electron-withdrawing or an electron-donating group Z, at positions on the aromatic substituent other than those adjacent the point of attachment to the dioxetane. Signal strength can further be enhanced by recognized chemiluminescent enhancers.

8. 发明授权

US5679803A 1,2 chemiluminescent dioxetanes of improved performance 失效
标题翻译： 1,2化学发光二恶烷具有改进的性能
公开(公告)号：US5679803A
公开(公告)日：1997-10-21
申请号：US547372
申请日：1995-10-25
申请人： Irena Bronstein , Brooks Edwards
发明人： Irena Bronstein , Brooks Edwards
IPC分类号： C07D305/14 , C07D321/00 , C07F9/06 , C07F9/655 , C07G3/00 , C12Q1/37 , G01N33/58 , C07F7/02
CPC分类号： C07D321/00 , C07F9/65512 , C07G3/00 , C12Q1/37 , G01N33/582 , Y02P20/55 , Y10S435/81
摘要： A new class of stable dioxetanes bears a polycyclic stabilizing group and aryloxy moiety, the oxygen atom of which is provided with a protective group which can be removed by an enzymatic or chemical trigger admixed with the dioxetane. The polycyclic stabilizing group is preferably spiroadamantane. The group further bears an alkoxy, aryloxy, aralkyloxy or cycloalkyloxymoiety which is partially or completely substituted with halogens, particularly fluorine and chlorine. Proper selection of electron active groups on the stabilizing moiety, the aryl group and the OR group yields enhanced enzyme kinetics, superior light intensity and excellent detection sensitivity in various assays.
摘要翻译：一类稳定的二氧杂环丁烷具有多环稳定基团和芳氧基部分，其氧原子具有可以通过与二氧杂环丁烷混合的酶或化学触发剂除去的保护基团。多环稳定基团优选为四金刚烷。该基团还具有部分或完全被卤素，特别是氟和氯取代的烷氧基，芳氧基，芳烷氧基或环烷氧基。稳定部分，芳基和OR基上的电子活性基团的适当选择在各种测定中产生增强的酶动力学，优异的光强度和优异的检测灵敏度。

9. 发明授权

US5582980A Chemiluminescent 1,2-dioxetanes 失效
标题翻译：化学发光的1,2-二氧杂环丁烷
公开(公告)号：US5582980A
公开(公告)日：1996-12-10
申请号：US231673
申请日：1994-04-25
申请人： Irena Bronstein , Brooks Edwards , Alison Sparks
发明人： Irena Bronstein , Brooks Edwards , Alison Sparks
IPC分类号： G01N33/532 , A43B3/00 , C07C43/178 , C07C43/196 , C07C43/215 , C07C43/225 , C07C43/23 , C07C43/315 , C07C45/67 , C07C47/565 , C07C47/575 , C07C65/03 , C07C65/21 , C07C69/00 , C07C69/017 , C07C69/24 , C07C69/78 , C07C69/84 , C07C69/92 , C07C205/06 , C07C205/37 , C07C205/44 , C07C217/84 , C07C233/18 , C07C233/75 , C07C271/28 , C07C271/58 , C07C309/75 , C07C311/04 , C07C311/08 , C07C311/16 , C07D321/00 , C07F7/08 , C07F7/18 , C07F9/12 , C07F9/40 , C07F9/655 , C07F9/6574 , C07G3/00 , C07H15/203 , C07H15/26 , C12Q1/34 , C12Q1/37 , C12Q1/42 , C12Q1/68 , G01N21/76 , G01N21/78 , G01N33/52 , G01N33/535 , G01N33/58 , C12Q1/00 , C07H15/24 , G01N33/53
CPC分类号： G01N33/582 , A43B1/0036 , A43B1/0072 , A43B3/0031 , C07C205/37 , C07C205/44 , C07C217/84 , C07C233/18 , C07C271/28 , C07C271/58 , C07C43/1788 , C07C43/196 , C07C43/215 , C07C43/225 , C07C43/23 , C07C43/315 , C07C45/673 , C07C47/565 , C07C47/575 , C07C65/03 , C07C65/21 , C07D321/00 , C07F9/12 , C07F9/4056 , C07F9/65512 , C07F9/65742 , C07G3/00 , C07H15/203 , C07H15/26 , C12Q1/34 , C12Q1/37 , C12Q1/42 , G01N21/76 , G01N33/581 , C07C2103/74 , C12Q2334/00 , Y10S435/81 , Y10S435/975
摘要： Spiroadamantyl dioxetanes bearing an alkoxy substituent, and a phenyl substituted on the dioxetane ring can be activated to chemiluminesce if the aromatic substituent bears a meta-substituted moiety designated OX, wherein the X is cleaved by an enzyme with which the dioxetane is permitted to come in contact with. The T.sub.1/2 kinetics of the chemiluminescent reaction, as well as the signal intensity and/or quantum yield of the chemiluminescent reaction, can be altered by addition of a chlorine substituent at position 4 on the phenyl ring. Signal strength can further be enhanced by recognized chemiluminescent enhancers.
摘要翻译：如果芳香族取代基具有指定为OX的间位取代的部分，则可以将带有烷氧基取代基的双金刚烷基二氧杂环丁烷和在二氧杂环丁烷环上取代的苯基活化成化学发光，其中X被允许进行二氧杂环丁烷的酶切割接触。化学发光反应的T + E，fra 1/2 + EE动力学以及化学发光反应的信号强度和/或量子产率可以通过在苯环上的第4位加入氯取代基来改变。信号强度可以通过认可的化学发光增强剂进一步增强。

10. 发明授权

US5336596A Membrane for chemiluminescent blotting applications 失效
标题翻译：用于化学发光印迹应用的膜
公开(公告)号：US5336596A
公开(公告)日：1994-08-09
申请号：US811620
申请日：1991-12-23
申请人： Irena Bronstein , Brooks Edwards , John Voyta
发明人： Irena Bronstein , Brooks Edwards , John Voyta
IPC分类号： G01N21/78 , B32B27/30 , C09D125/00 , C09D125/18 , G01N33/545 , G01N33/58 , C12B1/68 , B32B27/00
CPC分类号： G01N33/582 , G01N33/545 , Y10T428/31536 , Y10T428/31743 , Y10T428/31884
摘要： A new and improved polymeric membrane for use in biological assays is provided. A blotting assay employing 1,2-dioxetanes as a source of chemiluminescence employs, as an improved membrane, a polymer comprised of at least one monomer of the formula: ##STR1## The membranes reduce background signal, improve sensitivity and reliability.
摘要翻译：提供了用于生物测定的新的和改进的聚合物膜。使用1,2-二氧杂环丁烷作为化学发光源的印迹测定使用由至少一种下式的单体组成的聚合物作为改进的膜：提高了灵敏度和可靠性。

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